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Offlinetimothywyllie
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Contaminated syringe
    #27301965 - 05/09/21 06:35 PM (3 years, 8 months ago)

I have a syringe with spores that seem to be covered in a grey or black mold. I've tried multiple plates and when I think the mycelium is outrunning the mold it seems to always be connected to it. I've had good success with pf jars and bacterial syringes but I don't think that will work in this case. I need your help with any ideas you may have.

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Offlineverum subsequentis
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Re: Contaminated syringe [Re: timothywyllie]
    #27301971 - 05/09/21 06:40 PM (3 years, 8 months ago)

Got any pics of the plates? That'd help. If you want to try and up your chances use a loop and properly streak a few plates. It may very difficult to save though, if not impossible.

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Offlinetimothywyllie
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Re: Contaminated syringe [Re: verum subsequentis]
    #27301990 - 05/09/21 06:55 PM (3 years, 8 months ago)

Thanks, even when I take the outermost white it still turns black.

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OfflineJosborne
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Re: Contaminated syringe [Re: timothywyllie]
    #27302005 - 05/09/21 07:08 PM (3 years, 8 months ago)

when you say take outermost do you mean you've done agar transfers?

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Offlinetimothywyllie
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Re: Contaminated syringe [Re: Josborne]
    #27302016 - 05/09/21 07:15 PM (3 years, 8 months ago)

Quote:

Josborne said:
when you say take outermost do you mean you've done agar transfers?




Exactly. I've done about 10 transfers. Haha. Streaking a plate is a good idea.

Edited by timothywyllie (05/09/21 07:16 PM)

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InvisibleLadysKnight
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Re: Contaminated syringe [Re: timothywyllie]
    #27302026 - 05/09/21 07:25 PM (3 years, 8 months ago)

Yes, mold. I'd try a print


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Offlinetimothywyllie
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Re: Contaminated syringe [Re: LadysKnight]
    #27302035 - 05/09/21 07:38 PM (3 years, 8 months ago)

I don't have any prints. That's why I'm trying so hard to save the syringe.

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InvisibleLadysKnight
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Re: Contaminated syringe [Re: timothywyllie]
    #27302041 - 05/09/21 07:46 PM (3 years, 8 months ago)

Then maybe brf cakes/plates/pucks?


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Offlineverum subsequentis
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Re: Contaminated syringe [Re: LadysKnight]
    #27302085 - 05/09/21 08:26 PM (3 years, 8 months ago)

Mold does well on brf. I wouldn't try that. Timothy, Do you have any pics of the plates grown out a good bit?

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Offlinetimothywyllie
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Re: Contaminated syringe [Re: verum subsequentis]
    #27302851 - 05/10/21 10:56 AM (3 years, 8 months ago)

Yea it's a monster. Like Venom.
Basically does anything kill mold but not fungus?

Edited by timothywyllie (05/10/21 10:59 AM)

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Offlinemonkey_accident
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Re: Contaminated syringe [Re: timothywyllie]
    #27302899 - 05/10/21 11:35 AM (3 years, 8 months ago)

that white circle 5 oclock u have transferred?
looks like myc to me

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Offlineverum subsequentis
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Re: Contaminated syringe [Re: monkey_accident]
    #27302903 - 05/10/21 11:38 AM (3 years, 8 months ago)

Molds are fungi. They just have don't have to have sex to procreate. I'm not aware of anything that will kill "mold" but not "fungi". I think your plate is trash and possibly the syringe it came from is a complete loss.

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Offlinetimothywyllie
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Re: Contaminated syringe [Re: monkey_accident]
    #27303393 - 05/10/21 06:23 PM (3 years, 8 months ago)

Quote:

monkey_accident said:
that white circle 5 oclock u have transferred?
looks like myc to me



I'll keep an eye on it but to me it does not look like mycelium.  It's very similar to penicillium I've had before.  Likely to turn color in a minute.

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Invisiblesandman420
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Re: Contaminated syringe [Re: timothywyllie]
    #27303402 - 05/10/21 06:30 PM (3 years, 8 months ago)

this syringe is toast man. There isnt any good germination. You could just try streaking hundreds of plates with the full syringe, if there is anything good you should be able to find it lol.

or try brf jars they are contam resistant a little  bit but i mean yours looks fucked


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Edited by sandman420 (05/10/21 06:30 PM)

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Offlinetimothywyllie
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Re: Contaminated syringe [Re: sandman420]
    #27303437 - 05/10/21 06:58 PM (3 years, 8 months ago)

Si, thanks everyone for the ideas.

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