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Offlinexsirxx
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Registered: 05/10/21
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I have a question about whether this is the start of contamination or normal?
    #27303189 - 05/10/21 03:49 PM (3 years, 8 months ago)

First time poster!  Yayyyy!  I've lurked here for a VERY long time, so happy to finally be apart of the community.  Thanks in advance everyone!

So my question is, is the area in the circle contamination?  It popped up over night in about 8 hours.  This is the 4th day into FAE.

The tech is broke boi tek using only brown rice and coco.

If any more info is needed, I can provide it.  I wanted to say thanks again to everyone here, I have learned SOOO MUCH.










Edited by xsirxx (05/10/21 03:53 PM)

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Offlinetiptrippy
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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx]
    #27303199 - 05/10/21 04:01 PM (3 years, 8 months ago)

Just looks like mycelium to me. What's with that giant divot on the right side of your circle?

If you are misting, stop for a while and let some of that moisture evaporate away.

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Offlinexsirxx
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Re: I have a question about whether this is the start of contamination or normal? [Re: tiptrippy]
    #27303218 - 05/10/21 04:20 PM (3 years, 8 months ago)

Ahh yea thanks for the response, I should have mentioned that.  So I saw a very small about maybe an 1/8th of an inch of coloration that was most likely bruising from water drips off the side when I flipped the lid.  I wanted to remove it just in case, I also put a small amount of 3% hydrogen peroxide on it when I removed it.  That was the day after flipping the lid.  The following two days it was fine up until today when I saw a different type of formation growing.

This is my first time using broke boi tek.  My previous batches used PF Tek which I then turned into a mono tub and cheese grater using coco after my fruits were not producing enough.  That worked well but then with this batch and future ones, I wanted to skip the whole PF Tek and go straight to mono.  I should also mention I pasteurize my coco using a large sous vide.  When I did the PF Tek to mono using the grater it worked really well and my fruits went nuts.  Never had contamination before using that so I started to get worried.

Ohh to add, I havent misted yet.  I think my coco was too water heavy because the moisture level that you see is still from the original content.

Edited by xsirxx (05/10/21 04:21 PM)

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Offlinetiptrippy
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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx]
    #27303221 - 05/10/21 04:23 PM (3 years, 8 months ago)

The more I look at that spot the more its starting to look like early trich. Keep an eye on it

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Offlineverum subsequentis
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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx] * 1
    #27303223 - 05/10/21 04:23 PM (3 years, 8 months ago)

I hate to disagree and be the bearer of bad news but, if that ain't green in a day or two I'm a Russian midget hooker with three legs and one eye.

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Offlineverum subsequentis
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Re: I have a question about whether this is the start of contamination or normal? [Re: verum subsequentis]
    #27303225 - 05/10/21 04:24 PM (3 years, 8 months ago)

Beat me to it. Yes, you are correct.

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Offlinetiptrippy
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Re: I have a question about whether this is the start of contamination or normal? [Re: verum subsequentis]
    #27303226 - 05/10/21 04:24 PM (3 years, 8 months ago)

Quote:

verum subsequentis said:
I hate to disagree and be the bearer of bad news but, if that ain't green in a day or two I'm a Russian midget hooker with three legs and one eye.



Yeah I'm officially changing my mind. I don't think I was looking closely enough earlier.

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Offlineverum subsequentis
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Re: I have a question about whether this is the start of contamination or normal? [Re: tiptrippy]
    #27303229 - 05/10/21 04:27 PM (3 years, 8 months ago)

Word.

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Offlinexsirxx
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Re: I have a question about whether this is the start of contamination or normal? [Re: tiptrippy]
    #27303231 - 05/10/21 04:28 PM (3 years, 8 months ago)

And Now that you say that, I probably should have said earlier what I saw and removed.  My fault.

So is there a way to save this?  Or is it pretty much gone at this point?  Would removing it or half the tub work?

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Offlinetiptrippy
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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx]
    #27303254 - 05/10/21 04:42 PM (3 years, 8 months ago)

With how wet that tub is and how big the patch is, trich is probably colonizing way more than you can see. Best bet is to toss it out and start over.

But if you REALLY want to experiment, you can take a large bowl or food container and press it down into the sub all the way to the bottom. There is very very little chance of this working but I know some people can get attached to their grows

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Offlinexsirxx
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Re: I have a question about whether this is the start of contamination or normal? [Re: tiptrippy]
    #27303262 - 05/10/21 04:50 PM (3 years, 8 months ago)

I love experimenting so I am up for anything :smile:.  So the idea there would be to contain it but not remove it?

My next question is, how do I prevent this from happening again in the same room?  Is there a way to clean the room out so my next batch has a better chance?  Or is it more about letting the mycelium over take more before opening to fae?  My next batches I am using wbs instead of rice and the jars all look great so far.  So I guess more questions pop up... Is rice more susceptible to this than wbs?  Or is it the room that's contaminated?  I'd like to prevent as much as I can on the next batch.  If changing rooms is the best option, I can do that as well and try to clean the next room better before hand.  I also have large commercial air cleaners at my disposal.

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Offlinetiptrippy
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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx] * 1
    #27303267 - 05/10/21 04:51 PM (3 years, 8 months ago)

99% of the time if you get trich this early it is due to unclean spawn. The way to avoid it is to make sure your spawn is clean and healthy

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Offlineverum subsequentis
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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx] * 1
    #27303269 - 05/10/21 04:52 PM (3 years, 8 months ago)

Clean inoculant, proper sterile tek, proper sterilization. That's it. It was almost certainly from your spawn.

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Offlinexsirxx
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Re: I have a question about whether this is the start of contamination or normal? [Re: verum subsequentis]
    #27303293 - 05/10/21 05:06 PM (3 years, 8 months ago)

OK, so when you all say spawn, does that mean most likely in the jars ahead of time or in the coco?  What is strange is I used the exact same method with the coco on the pf tek when I turned them into mono.  Of course with the PF Tek I used BRF instead of just brown rice.  But that was really the only difference.

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InvisibleFeasoghorm

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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx]
    #27303324 - 05/10/21 05:27 PM (3 years, 8 months ago)


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OfflineSockadin
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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx]
    #27303350 - 05/10/21 05:38 PM (3 years, 8 months ago)

Quote:

xsirxx said:
OK, so when you all say spawn, does that mean most likely in the jars ahead of time or in the coco?  What is strange is I used the exact same method with the coco on the pf tek when I turned them into mono.  Of course with the PF Tek I used BRF instead of just brown rice.  But that was really the only difference.





If you shredded PF jars to your mono with Coir to make your "Spawn", than it was probably already in the PF jars. If you inoculated them with MS spore syringe, the jars would have molded over also. Spore syringes are dirty there isn't a good way around that. But I can assure you that smart people are working on that!

Edited by Sockadin (05/10/21 05:39 PM)

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Offlinexsirxx
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Re: I have a question about whether this is the start of contamination or normal? [Re: Sockadin]
    #27303375 - 05/10/21 05:58 PM (3 years, 8 months ago)

Haha I like that.  So I want to clarify that this tub was broke boi Tek.  My previous successful attempts were from brf pf Tek cheese grated into coco because they just weren't producing enough fruits.  After grating into coco, I had really good success.

But yea this time, which is my first failed attempt was from broke boi just brown flour and coco as substrate.  I want to add that I used my own prints and syringes for this batch.  So would it be safer to assume I probably introduced it at the syringe/print phase?

Sorry for so many questions, I just want to prevent as much for next time as possible.

So my next jars are from syringes I did not make(reputable), using wbs, and will go direct to coco.  I also used a new massive pressure cooker instead of my older one.  So maybe those changes will up my odds?  I really don't want to go back to pf Tek, so I'd like to eliminate all possible avenues of contamination.

Edited by xsirxx (05/10/21 06:00 PM)

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Offlinexsirxx
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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx]
    #27303464 - 05/10/21 07:28 PM (3 years, 8 months ago)

I think I have a better understanding at this point.  I wanted to thank you all again!  Best community ever!

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Invisiblearpeggiator
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Re: I have a question about whether this is the start of contamination or normal? [Re: xsirxx]
    #27303768 - 05/11/21 03:05 AM (3 years, 8 months ago)

Don't put spore syringes to grain.

Read this tek:
https://www.shroomery.org/forums/showflat.php/Number/24692374#24692374

And put your spore syringe to agar and get a clean culture going. It's intimidating, I know, but it's rewarding once you get it right. Your success rate will go way up. And I recommend reading and using the teks on shroomery instead of wherever you got broke boi tek from.

This list of links should have all you need to be successful

https://www.shroomery.org/forums/showflat.php?Cat=0&Number=24297804&page=0&vc=1#24297804


--------------------

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Offlinexsirxx
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Re: I have a question about whether this is the start of contamination or normal? [Re: arpeggiator]
    #27304252 - 05/11/21 12:03 PM (3 years, 8 months ago)

Definitely will read!  Thanks much.  I've read a lot on here.  Maybe not enough.  The broke boi tek started popping up during my searches across the internet and not sure where I finally settled on deciding to try it, I think it was YouTube.  I wanted to find a simpler method that would produce alot of fruits.  I ended up with none hah.

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