|
10kVisions
High, Real High.



Registered: 04/08/18
Posts: 188
|
can an entire room be contaminated?
#27302822 - 05/10/21 10:43 AM (3 years, 8 months ago) |
|
|
so if my agar looks good, jars look good, colonize well, to traces of anything green from what I can tell, my tubs are overly cleaned, and im following protocol inside sab etc.. BUT im getting green mold consistently at the FAE stage of monotubs. Could my room ha something in the air?
I work in a SAB for all stages but of course when mixing my tubs they're in that room and exposed to teh air etc etc.
after having 5 years of success and now almost 4 years of 1 out of 20 tubs flushing im beyond frustrated. BUT this is science, there has to be an answer that solves my problems.
all suggestions welcome. thanks
|
starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 1 year, 2 months
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27302881 - 05/10/21 11:16 AM (3 years, 8 months ago) |
|
|
If the pinset hasn't even come in and you're getting green mold that's a sign that you have bad spawn. Everyone around here goes through a similar experience once in awhile. Mold spores have a piss of a time germinating on coir, live mold mycelium does not so unless you've got a room full of living mold that you're actively putzing up into the air it's highly unlikely. If it's appearing once you introduce FAE that again tells me it's hitching a ride in your spawn.
Once you have a colonized jar throw a few grains of colonized grain onto a few plates of agar. I guarantee you that a few of those plates will have mold leap off the grain. Whenever I g2g from master jars to bags I like to randomly select a jar or two and put a few grains on agar now and some plates sure enough come back with green mold. Spawn looked fine, tubs fruited fine but contamination snuck in there on me.
Anyway just check your spawn on a few plates.
-------------------- Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.
 
|
10kVisions
High, Real High.



Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: starbones]
#27302898 - 05/10/21 11:34 AM (3 years, 8 months ago) |
|
|
Quote:
starbones said: If the pinset hasn't even come in and you're getting green mold that's a sign that you have bad spawn. Everyone around here goes through a similar experience once in awhile. Mold spores have a piss of a time germinating on coir, live mold mycelium does not so unless you've got a room full of living mold that you're actively putzing up into the air it's highly unlikely. If it's appearing once you introduce FAE that again tells me it's hitching a ride in your spawn.
Once you have a colonized jar throw a few grains of colonized grain onto a few plates of agar. I guarantee you that a few of those plates will have mold leap off the grain. Whenever I g2g from master jars to bags I like to randomly select a jar or two and put a few grains on agar now and some plates sure enough come back with green mold. Spawn looked fine, tubs fruited fine but contamination snuck in there on me.
Anyway just check your spawn on a few plates.
ya the interesting part is mold shows up right at the FAE stage... I 'll start to see baby pins sometimes and then it shows up. but its constiant right when pins would start growing green mold grows to. Now im dealign with 50 jars at a time so am I supposed to pop them ell and agar transfer them? cause that would take weeks longer to figure out then have to mark what agar plate goes with what jar etc etc etc... I mean I could but that just seems like a TON of extra work before a bulk spawn grow.
also, why would my agar look as good as it does as well as my colonizing jars and never once in the last 4 years show me mold in a jar etc... but never fail to get mold at the same stage every time? it jus baffles me.
|
starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 1 year, 2 months
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27302932 - 05/10/21 11:59 AM (3 years, 8 months ago) |
|
|
Nope, don't got to check all your jars. Just pop some randoms to agar before you spawn them and see if you had hidden contamination. This sounds like it's hiding in your cube myc in the spawn. Especially if you're noticing it when introducing FAE.
I rarely see green in the jar anymore. I know it's there when I check the spawn on agar or have the rare tub go green before the first flush. Some green molds show and tell with the available air exchange in a jar, some green molds don't.
-------------------- Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.
 
|
EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 6 hours, 6 minutes
|
Re: can an entire room be contaminated? [Re: starbones]
#27302941 - 05/10/21 12:05 PM (3 years, 8 months ago) |
|
|
That's interesting, is there any way mycelium can somehow Fuze with mold? Did you try starting a new calture or you are working with the same master?
|
10kVisions
High, Real High.



Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: starbones]
#27302947 - 05/10/21 12:08 PM (3 years, 8 months ago) |
|
|
Quote:
starbones said: Nope, don't got to check all your jars. Just pop some randoms to agar before you spawn them and see if you had hidden contamination. This sounds like it's hiding in your cube myc in the spawn. Especially if you're noticing it when introducing FAE.
I rarely see green in the jar anymore. I know it's there when I check the spawn on agar or have the rare tub go green before the first flush. Some green molds show and tell with the available air exchange in a jar, some green molds don't.
ok so the question would be what If I do find.. it. I jsut tossed 9 tubs due to this issue. if I check say 5 random jars out of 50 and they come back with green mold should I start over again? asking because if I put 9 tubs down again and they all go bad im gonna loose my mind haha
|
Eclipse3130
Servant of the Fungi



Registered: 10/06/13
Posts: 6,339
Loc: PNW
Last seen: 21 hours, 28 minutes
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27302948 - 05/10/21 12:09 PM (3 years, 8 months ago) |
|
|
Quote:
10kVisions said: BUT im getting green mold consistently at the FAE stage of monotubs. Could my room ha something in the air?
99% of contamination comes from spawn, the 1% being if you let your coir sit for months and months then there might be a chance something grew on it.
If your agar looks good, it's your spawn, make sure moisture levels are good, and you're venting PC properly. You could of G2Gd a dirty jar and unknowingly continued the cycle of spawning contams, remember trich is also mycelium before it turns green.
The best solution is to restart with a clean agar plate
-------------------- "In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"
|
10kVisions
High, Real High.



Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: Eclipse3130]
#27302958 - 05/10/21 12:17 PM (3 years, 8 months ago) |
|
|
Quote:
Eclipse3130 said:
Quote:
10kVisions said: BUT im getting green mold consistently at the FAE stage of monotubs. Could my room ha something in the air?
99% of contamination comes from spawn, the 1% being if you let your coir sit for months and months then there might be a chance something grew on it.
If your agar looks good, it's your spawn, make sure moisture levels are good, and you're venting PC properly. You could of G2Gd a dirty jar and unknowingly continued the cycle of spawning contams, remember trich is also mycelium before it turns green.
The best solution is to restart with a clean agar plate
my last jar 1 out of 50 whats wrong here?

my colonization in jars 99% of the time looks like this, whats Wrong here?

my agar plates on 3rd transfer, I use top right and one below that. whats wrong here?
|
Psicomb
monotub with eyes



Registered: 01/13/18
Posts: 4,917
Loc: WA
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27302959 - 05/10/21 12:18 PM (3 years, 8 months ago) |
|
|
Quote:
10kVisions said: so if my agar looks good, jars look good, colonize well, to traces of anything green from what I can tell, my tubs are overly cleaned, and im following protocol inside sab etc.. BUT im getting green mold consistently at the FAE stage of monotubs. Could my room ha something in the air?
I work in a SAB for all stages but of course when mixing my tubs they're in that room and exposed to teh air etc etc.
after having 5 years of success and now almost 4 years of 1 out of 20 tubs flushing im beyond frustrated. BUT this is science, there has to be an answer that solves my problems.
all suggestions welcome. thanks
Explain what you do for grain work please. What kinda PC do you have? Are you venting it for 10 minutes before putting the weight on? How long do you cook jars and bags?
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand
|
Psicomb
monotub with eyes



Registered: 01/13/18
Posts: 4,917
Loc: WA
|
Re: can an entire room be contaminated? [Re: Psicomb]
#27302962 - 05/10/21 12:19 PM (3 years, 8 months ago) |
|
|
Agar looks fine, first jar seems iffy but probably okay and second one seems alright. Is this happening with different varieties or are you just working one?
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand
|
10kVisions
High, Real High.


Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: Psicomb]
#27302971 - 05/10/21 12:31 PM (3 years, 8 months ago) |
|
|
Quote:
Psicomvb said: Agar looks fine, first jar seems iffy but probably okay and second one seems alright. Is this happening with different varieties or are you just working one?
happens no matter what variety I use, I've done cams, B+, EQ and a few more.. same thing same stage every time.
what looks "iffy" about the first jar? 2nd one is actually the first one but 2 weeks after a shake.
if my agar looks good, and im working in a SAB, sterilizing 100% between every transfer being honestly MORE meticulous than most people's TEK's on here... what am I doing wrong?
|
Psicomb
monotub with eyes



Registered: 01/13/18
Posts: 4,917
Loc: WA
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27302977 - 05/10/21 12:35 PM (3 years, 8 months ago) |
|
|
Are you using bags? Could your pressure gauge be defective? How long are your PC cycles?
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand
Edited by Psicomb (05/10/21 12:35 PM)
|
10kVisions
High, Real High.


Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: Psicomb]
#27302981 - 05/10/21 12:38 PM (3 years, 8 months ago) |
|
|
Quote:
Psicomvb said:
Quote:
10kVisions said: so if my agar looks good, jars look good, colonize well, to traces of anything green from what I can tell, my tubs are overly cleaned, and im following protocol inside sab etc.. BUT im getting green mold consistently at the FAE stage of monotubs. Could my room ha something in the air?
I work in a SAB for all stages but of course when mixing my tubs they're in that room and exposed to teh air etc etc.
after having 5 years of success and now almost 4 years of 1 out of 20 tubs flushing im beyond frustrated. BUT this is science, there has to be an answer that solves my problems.
all suggestions welcome. thanks
Explain what you do for grain work please. What kinda PC do you have? Are you venting it for 10 minutes before putting the weight on? How long do you cook jars and bags?
sorry I didn't see this. I have the classic presto pressure cooker, 3 of them. I run 10 jars at a time in each. Seed is as follow. WBS from Walmart, Penninton brand classic (least corn etc in it) I put 1 40lb bag into 2 5 gal buckets, cleaning out all "floaters" and rinsing till "clean". Then I fill up the bucket with the hottest tap I can put in it (my heater is set as high as It can go and will actually scold my hands) let sit for 24 hours after soaking, strain though strainers and cleaning as till water is clear. put in a tub and lightly dried using a towel. (due to my alt moisture Evaps fast so I need a more wet seed to start with). I don't have any pooling in my jars.
PC on and weight off, let sit till around 5psi then timed for 10min add weight adn raise to 19/20 psi for 2 hours. let cool and move jars into spawn room too cool down (spawn room is around 70º steady)Quote:
Psicomvb said: Are you using bags? Could your pressure gauge be defective? How long are your PC cycles?
no bags, I've replaced my gauges due to someone saying that years ago, but nothing changed.
|
give.more
Lurker


Registered: 04/30/16
Posts: 222
Loc: Copperhead Road
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27302985 - 05/10/21 12:40 PM (3 years, 8 months ago) |
|
|
Quote:
10kVisions said:
my last jar 1 out of 50 whats wrong here?

That jar looks like it might have yeast growing in it as well. I know my yeast from doing a lot of brewing and I've only had one jar grow it, so I'm not sure how correct I am on what I'm seeing.
It really might be ok, but that off color where the grains touch the glass makes me think yeast.
-------------------- Hi, I'm Dav. It was David but I lost my ID.
|
10kVisions
High, Real High.


Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: give.more]
#27302989 - 05/10/21 12:43 PM (3 years, 8 months ago) |
|
|
Quote:
give.more said:
Quote:
10kVisions said:
my last jar 1 out of 50 whats wrong here?

That jar looks like it might have yeast growing in it as well. I know my yeast from doing a lot of brewing and I've only had one jar grow it, so I'm not sure how correct I am on what I'm seeing.
It really might be ok, but that off color where the grains touch the glass makes me think yeast.
someone else brought that up due to doing seed and substrate in teh same buckets to I bought 3 igloo coolers (also to keep my temps up longer for substrate) and still got the same result. im not ruling it out of course... I just need to understand what im looking for and then of course why its happening.
|
10kVisions
High, Real High.


Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27302990 - 05/10/21 12:45 PM (3 years, 8 months ago) |
|
|
I took a photo of each side of that jar. here you go.
|
Psicomb
monotub with eyes



Registered: 01/13/18
Posts: 4,917
Loc: WA
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27302998 - 05/10/21 12:51 PM (3 years, 8 months ago) |
|
|
Yeah I mean your jars look pretty good. Hmm. I'm gonna sit on this issue and think for a little.
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand
|
10kVisions
High, Real High.


Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: Psicomb]
#27303001 - 05/10/21 12:53 PM (3 years, 8 months ago) |
|
|
Quote:
Psicomvb said: Yeah I mean your jars look pretty good. Hmm. I'm gonna sit on this issue and think for a little.
thanks, I went though some of the TEK's in your signature and there 100% the way I do things... down to the letter basically. this is what is baffling me ahah. I appreciate it. Feel free to PM me, im better one on one with stuff honestly.
going to make agar now.
|
Psicomb
monotub with eyes



Registered: 01/13/18
Posts: 4,917
Loc: WA
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27303006 - 05/10/21 12:56 PM (3 years, 8 months ago) |
|
|
For sure dude, I'll message you. Issues like this suck man, I feel your pain, a I montha after i switched to bags I tossed over 50 tubs before even getting a first flush cuz of PC issues, totally my fault, and I was focused on agar and grain prep and sterile work when really my shit just wasnt being PCd long enough. I would recommend maybe adding an extra hour to your cycle and seeing if it changes anything. 2 hrs with jars is plenty from my experience but still, never know I guess
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand
|
10kVisions
High, Real High.


Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: Psicomb]
#27303039 - 05/10/21 01:18 PM (3 years, 8 months ago) |
|
|
"exposed grain is a potential contam vector"
I don't use casing layers.. ever. never did back when I was having super successful grows so don't think to do it now. is there any truth to that statement? could my whole probably be based around exposed grains getting contains without that layer?
|
EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 6 hours, 6 minutes
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27303051 - 05/10/21 01:26 PM (3 years, 8 months ago) |
|
|
Bro maybe try a shoebox and see what's happening, one period for some reason my big monnos got contames af but shoeboxes worked fine lol :p I hope you'll find a solution soon update us, please!
Edited by EnDelfisE (05/10/21 01:26 PM)
|
Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
|
Re: can an entire room be contaminated? [Re: Psicomb]
#27303065 - 05/10/21 01:37 PM (3 years, 8 months ago) |
|
|
I know you've said you haven't seen green in your jars, have you tried leaving out uninoculated PC'd jars for a while to see whether anything is introduced during inoculation or if it's solely arising from your PC/lid set up?
What I've dealt with personally is green molds that have gotten into my jars presumably because of shitty filters/lids and have taken sometimes over 3 weeks to show themselves in uninoculated jars OR be overtaken by mycelium during colonization. What I had on my hands then was jars where I wasn't even sure if there was mold in it or not, because it often was such a small speck that the mycelium was able to completely overtake it and the only way I knew because the myc looked very stressed, or I had seen the spot of mold before it had been overtaken. I also had a lot of jars that looked okay, slightly bacterial, but upon opening it up there would be a few tiny spots of green mold on the top few grains. I probably threw out over 100 jars like that over a few months.
This is a rather different situation from yours as I was still mostly able to see it in the jars and your jars look better than mine did. For me, in the jars where the mycelium had overtaken the mold, when I spawned it to a shoebox just to test it out I was still able to get one flush before it shit the bed.
All this I guess is to say that there some molds can be sneaky as hell and that I'd try and rule out every possible vector for your spawn from your PC/lid set up/technique during inoculation before pursuing more uncommon explanations. I think you should definitely put some of your grain to agar and see what comes of that, you don't have to test every single jar you want to spawn but it will likely give you a better idea of where the issue might be coming from. If after all that you're confident your spawn is clean then yeah I'd maybe think about switching up random things, I know people have had issues mixing up their coir buckets with grain soak buckets or really rare cases like the water from their well being terrible or shit like that. That being said, if your spawn is indeed clean I really think it'd be able to get at least one flush out regardless of how dirty the room is. There's an old post by Mad Season IIRC where he spawned and fruited tubs right next to tubs basically full of trich and was still able to get a flush.
Hope you figure it out, throwing out tubs fucking sucks, it's a lot of work to get to the end and have it die like that, best of luck.
|
Psicomb
monotub with eyes



Registered: 01/13/18
Posts: 4,917
Loc: WA
|
Re: can an entire room be contaminated? [Re: Lenz]
#27303076 - 05/10/21 01:47 PM (3 years, 8 months ago) |
|
|
No that's not the issue, if anything not using a top layer just makes the surface conditions crummier than if you did but doesn't make anything more prone to contamination
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand
|
starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 1 year, 2 months
|
Re: can an entire room be contaminated? [Re: Psicomb] 1
#27303098 - 05/10/21 02:13 PM (3 years, 8 months ago) |
|
|
Jars can look perfectly fine and still have green mold riding on your mycelium. It doesn't take much. If you test your spawn like I told you, and I mean actually test multiple jars across multiple plates then you'll most likely find it in there. I said on the first page not all molds are readily apparent and not all will sporulate in the conditions a jar provides.
If you have 50 jars then randomly select 5, don't even look at them to see if you can tell which are fucked. Tap out of a few grains from each jar, do 5 plates per jar. It's in there, it's just not readily apparent.
Nobody on the internet can tell you if you have clean spawn by looking at a picture of a jar. They can only discern if you have obvious contamination. I wouldn't worry so much about the PCing aspect as the anal retentive part about that is more to do with bacteria than molds as we're trying to nuke endospore. Something is hitchhiking on your spawn it's nearly a certainty. Do you have any pictures of the contaminated tubs?
-------------------- Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.
 
|
Sockadin



Registered: 01/03/10
Posts: 7,266
Last seen: 1 month, 22 days
|
Re: can an entire room be contaminated? [Re: starbones]
#27303287 - 05/10/21 05:03 PM (3 years, 8 months ago) |
|
|
It is the coir. FUCK IT im saying it is in the coir. Eco Earth Sucks. I just bought a 3 pack and I fully plan on Pressure Cooking it before using. I have had way to much crap show up in the compressed bricks.
I know alot of people are going to blame the grain prep. Or rely on the old "it's your spawn" but sometimes it is the coir. I might get shit for that.
Experiment and spawn 1 quart of your spawn to straight Vermiculite in a little container and see if it turns green.
|
sandman420
Saint PP



Registered: 06/17/04
Posts: 5,387
|
Re: can an entire room be contaminated? [Re: Sockadin]
#27303377 - 05/10/21 06:01 PM (3 years, 8 months ago) |
|
|
What is your air conditioner situation
|
Benson
The Kidd ⭐


Registered: 09/29/20
Posts: 898
|
Re: can an entire room be contaminated? [Re: EnDelfisE]
#27303440 - 05/10/21 06:59 PM (3 years, 8 months ago) |
|
|
Quote:
EnDelfisE said: is there any way mycelium can somehow Fuze with mold?
I've been looking for the answer to this for so long, I swear there is something riding along on my GT myc
-------------------- [||||||||||||||||||||||||||||||||||||]
[||||||||||||||||||||||||||||||||||||]
[||||||||||||||||||||||||||||||||||||]
|
10kVisions
High, Real High.



Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: Benson]
#27303524 - 05/10/21 08:44 PM (3 years, 8 months ago) |
|
|
Quote:
Benson said:
Quote:
EnDelfisE said: is there any way mycelium can somehow Fuze with mold?
I've been looking for the answer to this for so long, I swear there is something riding along on my GT myc 
im about to do some pretty extensive testing using shoe box method grows to try and eliminate and pinpoint my issues. I'll be keeping a journal about it.
|
trubblesome
Stranger



Registered: 11/09/19
Posts: 406
Last seen: 1 year, 10 months
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27303589 - 05/10/21 09:48 PM (3 years, 8 months ago) |
|
|
whats your coir prep like?
I switched to growing in myco bags mostly and went to 99% success rate (had one bag green, it was sketch spawn) despite nothing else changing except coir prep - tea kettle, pour in bag vs boil a fuckload of water, pour in bucket.
then I realized i'd been prepping coir for tubs in the same bucket I used to drain oats, haul random supplies in, etc, and used the same big pot I prepped oats in to boil water. pretty much no matter how hard I scrubbed or bleached or whatever, residue from the oats and whatever else shit would make it on to my coir - perfect nutrition source for mold. now i scour the fuck out of my big stock pot before boiling water for coir, and use a separate, coir only bucket for coir prep. no problems since.
--------------------
|
10kVisions
High, Real High.



Registered: 04/08/18
Posts: 188
|
Re: can an entire room be contaminated? [Re: trubblesome]
#27303640 - 05/10/21 11:11 PM (3 years, 8 months ago) |
|
|
Quote:
trubblesome said: whats your coir prep like?
I switched to growing in myco bags mostly and went to 99% success rate (had one bag green, it was sketch spawn) despite nothing else changing except coir prep - tea kettle, pour in bag vs boil a fuckload of water, pour in bucket.
then I realized i'd been prepping coir for tubs in the same bucket I used to drain oats, haul random supplies in, etc, and used the same big pot I prepped oats in to boil water. pretty much no matter how hard I scrubbed or bleached or whatever, residue from the oats and whatever else shit would make it on to my coir - perfect nutrition source for mold. now i scour the fuck out of my big stock pot before boiling water for coir, and use a separate, coir only bucket for coir prep. no problems since.
thanks for the reply, I've tested 5 difffent types of bricks and used both my WBS buckets to make coir and brand new untouched cleaned with alcohol buckets and igloo water coolers all with the same result. I've worked out an idea with some of the trusted cultivators on here to narrow down whats going on. we'll figure it out for sure.
|
PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 2 years, 4 months
|
Re: can an entire room be contaminated? [Re: Benson]
#27304544 - 05/11/21 04:14 PM (3 years, 8 months ago) |
|
|
Quote:
Benson said:
Quote:
EnDelfisE said: is there any way mycelium can somehow Fuze with mold?
I've been looking for the answer to this for so long, I swear there is something riding along on my GT myc 
It can do this for sure, trich certainly does this and waits for the right conditions while it nestles along the mycelium. Just note that even if you go to clean the myc up it's not always 100%. i.e. you can transfer away from mold multiple times and the same stuff will reappear, so, better to start over from spores or clean clone.
Also check this out - I have no particular proof other than logic - if I take a clone from a commercial mushroom strain (maitake, lions mane) and the transfer is done cleanly, I get no contams on the clone plate. If I take a clone from a possibly infected cube fruit (with same conditions) often contams show up as well. To me this indicates the contams ride along on the myc, and they ride along on the myc INTO the fruits, where they can still be propagated from.
My best results have always come from the best and cleanest myc, absolutely no doubt. You know it when you see it. 
Quote:
10kVisions said: can an entire room be contaminated?
OK, OP, in answer to that original question, yes, your fruiting environment can become contaminated. This kind of thing has driven some people almost around the bend. In one case they had to move away before their grows worked again. If trich gets a foothold in the environment of the room you're fruiting in, it never wants to give that up again. It's like the trich's wet dream come true.
But the spores have a limited life, and if you stop growing in an area for a few (maybe 5?) years and you go back at it again the trich problems will have ceased. There are ways to disinfect a room, of varying and dubious efficacy, as some people can describe much better.
So one simple method to test would be to make a little grow-kit thing and give it to a trusted friend to fruit in someplace-that's-not-your-house. Or spread the fruiting area out into new areas of an existing house. Which may not work for everybody.
--------------------
if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
|
sandman420
Saint PP



Registered: 06/17/04
Posts: 5,387
|
Re: can an entire room be contaminated? [Re: PrimalSoup]
#27304561 - 05/11/21 04:27 PM (3 years, 8 months ago) |
|
|
You can disinfect a room with a hardcore lengthy exposure of ozone from an ozone generator.
Here is a scientific article on trich going mycoparasite. https://scialert.net/fulltext/?doi=ajppaj.2016.21.28
|
monkey_accident
seraphim


Registered: 05/10/19
Posts: 710
Loc: im here, bro
Last seen: 4 months, 9 days
|
Re: can an entire room be contaminated? [Re: Sockadin]
#27304647 - 05/11/21 05:27 PM (3 years, 8 months ago) |
|
|
Quote:
Sockadin said: It is the coir. FUCK IT im saying it is in the coir.
I had a long string of contamination - tossing 10+ monos dead to trich BEFORE they started pinning after trying everything i thought it could be the coir and bought a new pack of bricks and no more tam after that  mix clean spawn with dirty coir and id say youve got a less than favorable chance of a first flush this goes without saying that there are SO many vectors for contamination so try to limit them as much as possible test your grain on agar (clean spawn check), pc your coir / buy new bricks / test your coir - and dont waste more resources than necessary until you figure it out and get a clean grow
|
NaMeLeSs_FcUk89
Stranger

Registered: 10/06/19
Posts: 12
Last seen: 3 years, 2 months
|
|
Lol... I’m literally having the same issue bro!! I’ve been doing this for 8 years now and for some reason I’m only able to get 1 solid flush out of my tubs. I’ve changed everything more than once and was right where your at thinking I had contaminated my whole grow space. Then after fogging whole room with bleach water several times and starting over I kept getting the green shit. Then I have been reading that it’s because I’m only using coco and verm and for more flushes requires some kind of poo and the substrate to be buffered. Idk but I know for a fact that I was getting 4 monster flushes using coco/verm and a little gypsum as a buffer, I’m not having it as bad as you man but it sucks doing it on the level we’re doing it at and only being able to get 1 fucking flush!! I do 15 tubs a month to get the product that I need but if I could get more than 1 flush I would only have to do 5. I will say this though I bought some brand new tubs and I’m feeling pretty good about them man, I bought 5 new ones and they’re perfectly colonizied and cased about to go into fruiting. I’ve been trying to figure out this issue for over a year now. I get first flushes like this every time but I can’t seem to figure out why I can’t get a 2nd flush befor me contaminating.
|
NaMeLeSs_FcUk89
Stranger

Registered: 10/06/19
Posts: 12
Last seen: 3 years, 2 months
|
|
I was also wondering about the coir thing cause it does seem that ever since I started buying the big compressed blocks from the hydro store this issue has arrised. I have changed blocks but not back to eco earth cause it’s so expensive when your buying 20 packs of them. But wtf is pasteurization for then?? I’ve read several times that some hydro store coco that’s for growing cannabis is treated with trich but why would it matter if we’re pasterizing or sterilizing?
|
TheOffice
Stranger



Registered: 05/13/21
Posts: 93
Loc: The Netherlands
Last seen: 4 months, 16 days
|
Re: can an entire room be contaminated? [Re: 10kVisions]
#27312670 - 05/17/21 02:49 PM (3 years, 7 months ago) |
|
|
Not claiming anything, but just trying to help out.
I had the same problem, ( only difference is that i use grains instead of WBS, which maybe changes everything)
But for me things went way better when i stopped soaking my grains on advice of BOD.
https://www.shroomery.org/forums/showflat.php/Number/21604725
You could at least give it a try.
Good luck brother
-------------------- Cum grege non gradior
|
|