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OfflineEnDelfisE
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27303051 - 05/10/21 01:26 PM (3 years, 8 months ago)

Bro maybe try a shoebox and see what's happening, one period for some reason my big monnos got contames af but shoeboxes worked fine lol :p
I hope you'll find a solution soon update us, please!

Edited by EnDelfisE (05/10/21 01:26 PM)

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InvisibleLenz
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Registered: 08/17/20
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Re: can an entire room be contaminated? [Re: Psicomb]
    #27303065 - 05/10/21 01:37 PM (3 years, 8 months ago)

I know you've said you haven't seen green in your jars, have you tried leaving out uninoculated PC'd jars for a while to see whether anything is introduced during inoculation or if it's solely arising from your PC/lid set up?

What I've dealt with personally is green molds that have gotten into my jars presumably because of shitty filters/lids and have taken sometimes over 3 weeks to show themselves in uninoculated jars OR be overtaken by mycelium during colonization. What I had on my hands then was jars where I wasn't even sure if there was mold in it or not, because it often was such a small speck that the mycelium was able to completely overtake it and the only way I knew because the myc looked very stressed, or I had seen the spot of mold before it had been overtaken. I also had a lot of jars that looked okay, slightly bacterial, but upon opening it up there would be a few tiny spots of green mold on the top few grains. I probably threw out over 100 jars like that over a few months.

This is a rather different situation from yours as I was still mostly able to see it in the jars and your jars look better than mine did. For me, in the jars where the mycelium had overtaken the mold, when I spawned it to a shoebox just to test it out I was still able to get one flush before it shit the bed.

All this I guess is to say that there some molds can be sneaky as hell and that I'd try and rule out every possible vector for your spawn from your PC/lid set up/technique during inoculation before pursuing more uncommon explanations. I think you should definitely put some of your grain to agar and see what comes of that, you don't have to test every single jar you want to spawn but it will likely give you a better idea of where the issue might be coming from. If after all that you're confident your spawn is clean then yeah I'd maybe think about switching up random things, I know people have had issues mixing up their coir buckets with grain soak buckets or really rare cases like the water from their well being terrible or shit like that. That being said, if your spawn is indeed clean I really think it'd be able to get at least one flush out regardless of how dirty the room is. There's an old post by Mad Season IIRC where he spawned and fruited tubs right next to tubs basically full of trich and was still able to get a flush.

Hope you figure it out, throwing out tubs fucking sucks, it's a lot of work to get to the end and have it die like that, best of luck. :thumbup:


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:chems:

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InvisiblePsicomb
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Re: can an entire room be contaminated? [Re: Lenz]
    #27303076 - 05/10/21 01:47 PM (3 years, 8 months ago)

No that's not the issue, if anything not using a top layer just makes the surface conditions crummier than if you did but doesn't make anything more prone to contamination


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Offlinestarbones
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Re: can an entire room be contaminated? [Re: Psicomb] * 1
    #27303098 - 05/10/21 02:13 PM (3 years, 8 months ago)

Jars can look perfectly fine and still have green mold riding on your mycelium. It doesn't take much. If you test your spawn like I told you, and I mean actually test multiple jars across multiple plates then you'll most likely find it in there. I said on the first page not all molds are readily apparent and not all will sporulate in the conditions a jar provides.

If you have 50 jars then randomly select 5, don't even look at them to see if you can tell which are fucked. Tap out of a few grains from each jar, do 5 plates per jar. It's in there, it's just not readily apparent.

Nobody on the internet can tell you if you have clean spawn by looking at a picture of a jar. They can only discern if you have obvious contamination. I wouldn't worry so much about the PCing aspect as the anal retentive part about that is more to do with bacteria than molds as we're trying to nuke endospore. Something is hitchhiking on your spawn it's nearly a certainty.
Do you have any pictures of the contaminated tubs?


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OfflineSockadin
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Re: can an entire room be contaminated? [Re: starbones]
    #27303287 - 05/10/21 05:03 PM (3 years, 8 months ago)

It is the coir. FUCK IT im saying it is in the coir. Eco Earth Sucks. I just bought a 3 pack and I fully plan on Pressure Cooking it before using. I have had way to much crap show up in the compressed bricks.

I know alot of people are going to blame the grain prep. Or rely on the old "it's your spawn" but sometimes it is the coir. I might get shit for that.

  Experiment and spawn 1 quart of your spawn to straight Vermiculite in a little container and see if it turns green.

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Invisiblesandman420
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Re: can an entire room be contaminated? [Re: Sockadin]
    #27303377 - 05/10/21 06:01 PM (3 years, 8 months ago)

What is your air conditioner situation


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InvisibleBenson
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Re: can an entire room be contaminated? [Re: EnDelfisE]
    #27303440 - 05/10/21 06:59 PM (3 years, 8 months ago)

Quote:

EnDelfisE said:
is there any way mycelium can somehow Fuze with mold?




I've been looking for the answer to this for so long, I swear there is something riding along on my GT myc :dead:


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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: Benson]
    #27303524 - 05/10/21 08:44 PM (3 years, 8 months ago)

Quote:

Benson said:
Quote:

EnDelfisE said:
is there any way mycelium can somehow Fuze with mold?




I've been looking for the answer to this for so long, I swear there is something riding along on my GT myc :dead:




im about to do some pretty extensive testing using shoe box method grows to try and eliminate and pinpoint my issues.  I'll be keeping a journal about it.


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Offlinetrubblesome
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27303589 - 05/10/21 09:48 PM (3 years, 8 months ago)

whats your coir prep like?

I switched to growing in myco bags mostly and went to 99% success rate (had one bag green, it was sketch spawn) despite nothing else changing except coir prep - tea kettle, pour in bag vs boil a fuckload of water, pour in bucket.

then I realized i'd been prepping coir for tubs in the same bucket I used to drain oats, haul random supplies in, etc, and used the same big pot I prepped oats in to boil water. pretty much no matter how hard I scrubbed or bleached or whatever, residue from the oats and whatever else shit would make it on to my coir - perfect nutrition source for mold. now i scour the fuck out of my big stock pot before boiling water for coir, and use a separate, coir only bucket for coir prep. no problems since.


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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: trubblesome]
    #27303640 - 05/10/21 11:11 PM (3 years, 8 months ago)

Quote:

trubblesome said:
whats your coir prep like?

I switched to growing in myco bags mostly and went to 99% success rate (had one bag green, it was sketch spawn) despite nothing else changing except coir prep - tea kettle, pour in bag vs boil a fuckload of water, pour in bucket.

then I realized i'd been prepping coir for tubs in the same bucket I used to drain oats, haul random supplies in, etc, and used the same big pot I prepped oats in to boil water. pretty much no matter how hard I scrubbed or bleached or whatever, residue from the oats and whatever else shit would make it on to my coir - perfect nutrition source for mold. now i scour the fuck out of my big stock pot before boiling water for coir, and use a separate, coir only bucket for coir prep. no problems since.




thanks for the reply, I've tested 5 difffent types of bricks and used both my WBS buckets to make coir and brand new untouched cleaned with alcohol buckets and igloo water coolers all with the same result.  I've worked out an idea with some of the trusted cultivators on here to narrow down whats going on. we'll figure it out for sure.


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OfflinePrimalSoup
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Re: can an entire room be contaminated? [Re: Benson]
    #27304544 - 05/11/21 04:14 PM (3 years, 8 months ago)

Quote:

Benson said:
Quote:

EnDelfisE said:
is there any way mycelium can somehow Fuze with mold?




I've been looking for the answer to this for so long, I swear there is something riding along on my GT myc :dead:




It can do this for sure, trich certainly does this and waits for the right conditions while it nestles along the mycelium.  Just note that even if you go to clean the myc up it's not always 100%.  i.e. you can transfer away from mold multiple times and the same stuff will reappear, so, better to start over from spores or clean clone.

Also check this out - I have no particular proof other than logic - if I take a clone from a commercial mushroom strain (maitake, lions mane) and the transfer is done cleanly, I get no contams on the clone plate.  If I take a clone from a possibly infected cube fruit (with same conditions) often contams show up as well.  To me this indicates the contams ride along on the myc, and they ride along on the myc INTO the fruits, where they can still be propagated from.

My best results have always come from the best and cleanest myc, absolutely no doubt. You know it when you see it. :havesomescience:

Quote:

10kVisions said:
can an entire room be contaminated?




OK, OP, in answer to that original question, yes, your fruiting environment can become contaminated.  This kind of thing has driven some people almost around the bend.  In one case they had to move away before their grows worked again.  If trich gets a foothold in the environment of the room you're fruiting in, it never wants to give that up again. It's like the trich's wet dream come true.

But the spores have a limited life, and if you stop growing in an area for a few (maybe 5?) years and you go back at it again the trich problems will have ceased.  There are ways to disinfect a room, of varying and dubious efficacy, as some people can describe much better.

So one simple method to test would be to make a little grow-kit thing and give it to a trusted friend to fruit in someplace-that's-not-your-house.  Or spread the fruiting area out into new areas of an existing house.  Which may not work for everybody. :laugh2:


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Invisiblesandman420
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Re: can an entire room be contaminated? [Re: PrimalSoup]
    #27304561 - 05/11/21 04:27 PM (3 years, 8 months ago)

You can disinfect a room with a hardcore lengthy exposure of ozone from an ozone generator.

Here is a scientific article on trich going mycoparasite. https://scialert.net/fulltext/?doi=ajppaj.2016.21.28


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Offlinemonkey_accident
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Re: can an entire room be contaminated? [Re: Sockadin]
    #27304647 - 05/11/21 05:27 PM (3 years, 8 months ago)

Quote:

Sockadin said:
It is the coir. FUCK IT im saying it is in the coir.




I had a long string of contamination - tossing 10+ monos dead to trich BEFORE they started pinning
after trying everything i thought it could be the coir and bought a new pack of bricks and no more tam after that :shrug:
mix clean spawn with dirty coir and id say youve got a less than favorable chance of a first flush
this goes without saying that there are SO many vectors for contamination so try to limit them as much as possible
test your grain on agar (clean spawn check), pc your coir / buy new bricks / test your coir -
and dont waste more resources than necessary until you figure it out and get a clean grow


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OfflineNaMeLeSs_FcUk89
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Re: can an entire room be contaminated? [Re: monkey_accident]
    #27308169 - 05/14/21 10:27 AM (3 years, 8 months ago)

Lol... I’m literally having the same issue bro!! I’ve been doing this for 8 years now and for some reason I’m only able to get 1 solid flush out of my tubs. I’ve changed everything more than once and was right where your at thinking I had contaminated my whole grow space. Then after fogging whole room with bleach water several times and starting over I kept getting the green shit. Then I have been reading that it’s because I’m only using coco and verm and for more flushes requires some kind of poo and the substrate to be buffered. Idk but I know for a fact that I was getting 4 monster flushes using coco/verm and a little gypsum as a buffer, I’m not having it as bad as you man but it sucks doing it on the level we’re doing it at and only being able to get 1 fucking flush!! I do 15 tubs a month to get the product that I need but if I could get more than 1 flush I would only have to do 5. I will say this though I bought some brand new tubs and I’m feeling pretty good about them man, I bought 5 new ones and they’re perfectly colonizied and cased about to go into fruiting. I’ve been trying to figure out this issue for over a year now.
I get first flushes like this every time but I can’t seem to figure out why I can’t get a 2nd flush befor me contaminating.

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OfflineNaMeLeSs_FcUk89
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Re: can an entire room be contaminated? [Re: NaMeLeSs_FcUk89]
    #27308189 - 05/14/21 10:40 AM (3 years, 8 months ago)

I was also wondering about the coir thing cause it does seem that ever since I started buying the big compressed blocks from the hydro store this issue has arrised. I have changed blocks but not back to eco earth cause it’s so expensive when your buying 20 packs of them. But wtf is pasteurization for then?? I’ve read several times that some hydro store coco that’s for growing cannabis is treated with trich but why would it matter if we’re pasterizing or sterilizing?

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OfflineTheOffice
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27312670 - 05/17/21 02:49 PM (3 years, 7 months ago)

Not claiming anything, but just trying to help out.

I had the same problem, ( only difference is that i use grains instead of WBS, which maybe changes everything)

But for me things went way better when i stopped soaking my grains on advice of BOD.

https://www.shroomery.org/forums/showflat.php/Number/21604725

You could at least give it a try.

Good luck brother


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Cum grege non gradior






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