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Invisible10kVisions
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can an entire room be contaminated?
    #27302822 - 05/10/21 10:43 AM (3 years, 8 months ago)

so if my agar looks good, jars look good, colonize well, to traces of anything green from what I can tell, my tubs are overly cleaned, and im following protocol inside sab etc.. BUT im getting green mold consistently at the FAE stage of monotubs.  Could my room ha something in the air?

I work in a SAB for all stages but of course when mixing my tubs they're in that room and exposed to teh air etc etc.

after having 5 years of success and now almost 4 years of 1 out of 20 tubs flushing im beyond frustrated.  BUT  this is science, there has to be an answer that solves my problems.

all suggestions welcome.
thanks


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Offlinestarbones
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27302881 - 05/10/21 11:16 AM (3 years, 8 months ago)

If the pinset hasn't even come in and you're getting green mold that's a sign that you have bad spawn. Everyone around here goes through a similar experience once in awhile.
Mold spores have a piss of a time germinating on coir, live mold mycelium does not so unless you've got a room full of living mold that you're actively putzing up into the air it's highly unlikely. If it's appearing once you introduce FAE that again tells me it's hitching a ride in your spawn.

Once you have a colonized jar throw a few grains of colonized grain onto a few plates of agar. I guarantee you that a few of those plates will have mold leap off the grain. Whenever I g2g from master jars to bags I like to randomly select a jar or two and put a few grains on agar now and some plates sure enough come back with green mold. Spawn looked fine, tubs fruited fine but contamination snuck in there on me.

Anyway just check your spawn on a few plates.


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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: starbones]
    #27302898 - 05/10/21 11:34 AM (3 years, 8 months ago)

Quote:

starbones said:
If the pinset hasn't even come in and you're getting green mold that's a sign that you have bad spawn. Everyone around here goes through a similar experience once in awhile.
Mold spores have a piss of a time germinating on coir, live mold mycelium does not so unless you've got a room full of living mold that you're actively putzing up into the air it's highly unlikely. If it's appearing once you introduce FAE that again tells me it's hitching a ride in your spawn.

Once you have a colonized jar throw a few grains of colonized grain onto a few plates of agar. I guarantee you that a few of those plates will have mold leap off the grain. Whenever I g2g from master jars to bags I like to randomly select a jar or two and put a few grains on agar now and some plates sure enough come back with green mold. Spawn looked fine, tubs fruited fine but contamination snuck in there on me.

Anyway just check your spawn on a few plates.




ya the interesting part is mold shows up right at the FAE stage... I 'll start to see baby pins sometimes and then it shows up.  but its constiant right when pins would start growing green mold grows to.  Now im dealign with 50 jars at a time so am I supposed to pop them ell and agar transfer them?  cause that would take weeks longer to figure out then have to mark what agar plate goes with what jar etc etc etc... I mean I could but that just seems like a TON of extra work before a bulk spawn grow.

also, why would my agar look as good as it does as well as my colonizing jars and never once in the last 4 years show me mold in a jar etc... but never fail to get mold at the same stage every time?  it jus baffles me.


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Offlinestarbones
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27302932 - 05/10/21 11:59 AM (3 years, 8 months ago)

Nope, don't got to check all your jars. Just pop some randoms to agar before you spawn them and see if you had hidden contamination. This sounds like it's hiding in your cube myc in the spawn. Especially if you're noticing it when introducing FAE.

I rarely see green in the jar anymore. I know it's there when I check the spawn on agar or have the rare tub go green before the first flush. Some green molds show and tell with the available air exchange in a jar, some green molds don't.


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OfflineEnDelfisE
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Re: can an entire room be contaminated? [Re: starbones]
    #27302941 - 05/10/21 12:05 PM (3 years, 8 months ago)

That's interesting, is there any way mycelium can somehow Fuze with mold?  Did you try starting a new calture or you are working with the same master?

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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: starbones]
    #27302947 - 05/10/21 12:08 PM (3 years, 8 months ago)

Quote:

starbones said:
Nope, don't got to check all your jars. Just pop some randoms to agar before you spawn them and see if you had hidden contamination. This sounds like it's hiding in your cube myc in the spawn. Especially if you're noticing it when introducing FAE.

I rarely see green in the jar anymore. I know it's there when I check the spawn on agar or have the rare tub go green before the first flush. Some green molds show and tell with the available air exchange in a jar, some green molds don't.




ok so the question would be what If I do find.. it.  I jsut tossed 9 tubs due to this issue. if I check say 5 random jars out of 50 and they come back with green mold should I start over again?  asking because if I put 9 tubs down again and they all go bad im gonna loose my mind haha


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OfflineEclipse3130
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27302948 - 05/10/21 12:09 PM (3 years, 8 months ago)

Quote:

10kVisions said:
BUT im getting green mold consistently at the FAE stage of monotubs.  Could my room ha something in the air?





99% of contamination comes from spawn, the 1% being if you let your coir sit for months and months then there might be a chance something grew on it.

If your agar looks good, it's your spawn, make sure moisture levels are good, and you're venting PC properly. You could of G2Gd a dirty jar and unknowingly continued the cycle of spawning contams, remember trich is also mycelium before it turns green.

The best solution is to restart with a clean agar plate


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In The Magical World One seeks Enlightenment and grows Wiser
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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: Eclipse3130]
    #27302958 - 05/10/21 12:17 PM (3 years, 8 months ago)

Quote:

Eclipse3130 said:
Quote:

10kVisions said:
BUT im getting green mold consistently at the FAE stage of monotubs.  Could my room ha something in the air?





99% of contamination comes from spawn, the 1% being if you let your coir sit for months and months then there might be a chance something grew on it.

If your agar looks good, it's your spawn, make sure moisture levels are good, and you're venting PC properly. You could of G2Gd a dirty jar and unknowingly continued the cycle of spawning contams, remember trich is also mycelium before it turns green.

The best solution is to restart with a clean agar plate




my last jar 1 out of 50 whats wrong here?


my colonization in jars 99% of the time looks like this, whats Wrong here?


my agar plates on 3rd transfer, I use top right and one below that. whats wrong here?


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InvisiblePsicomb
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27302959 - 05/10/21 12:18 PM (3 years, 8 months ago)

Quote:

10kVisions said:
so if my agar looks good, jars look good, colonize well, to traces of anything green from what I can tell, my tubs are overly cleaned, and im following protocol inside sab etc.. BUT im getting green mold consistently at the FAE stage of monotubs.  Could my room ha something in the air?

I work in a SAB for all stages but of course when mixing my tubs they're in that room and exposed to teh air etc etc.

after having 5 years of success and now almost 4 years of 1 out of 20 tubs flushing im beyond frustrated.  BUT  this is science, there has to be an answer that solves my problems.

all suggestions welcome.
thanks



Explain what you do for grain work please.  What kinda PC do you have? Are you venting it for 10 minutes before putting the weight on?  How long do you cook jars and bags?


--------------------

When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand

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InvisiblePsicomb
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Re: can an entire room be contaminated? [Re: Psicomb]
    #27302962 - 05/10/21 12:19 PM (3 years, 8 months ago)

Agar looks fine, first jar seems iffy but probably okay and second one seems alright.  Is this happening with different varieties or are you just working one?


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand

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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: Psicomb]
    #27302971 - 05/10/21 12:31 PM (3 years, 8 months ago)

Quote:

Psicomvb said:
Agar looks fine, first jar seems iffy but probably okay and second one seems alright.  Is this happening with different varieties or are you just working one?




happens no matter what variety I use, I've done cams, B+, EQ and a few more.. same thing same stage every time. 

what looks "iffy" about the first jar?  2nd one is actually the first one but 2 weeks after a shake.

if my agar looks good, and im working in a SAB, sterilizing 100% between every transfer being honestly MORE meticulous than most people's TEK's on here... what am I doing wrong?


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InvisiblePsicomb
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27302977 - 05/10/21 12:35 PM (3 years, 8 months ago)

Are you using bags? Could your pressure gauge be defective?  How long are your PC cycles?



--------------------

When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand

Edited by Psicomb (05/10/21 12:35 PM)

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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: Psicomb]
    #27302981 - 05/10/21 12:38 PM (3 years, 8 months ago)

Quote:

Psicomvb said:
Quote:

10kVisions said:
so if my agar looks good, jars look good, colonize well, to traces of anything green from what I can tell, my tubs are overly cleaned, and im following protocol inside sab etc.. BUT im getting green mold consistently at the FAE stage of monotubs.  Could my room ha something in the air?

I work in a SAB for all stages but of course when mixing my tubs they're in that room and exposed to teh air etc etc.

after having 5 years of success and now almost 4 years of 1 out of 20 tubs flushing im beyond frustrated.  BUT  this is science, there has to be an answer that solves my problems.

all suggestions welcome.
thanks



Explain what you do for grain work please.  What kinda PC do you have? Are you venting it for 10 minutes before putting the weight on?  How long do you cook jars and bags?





sorry I didn't see this. I have the classic presto pressure cooker, 3 of them. I run 10 jars at a time in each.
Seed is as follow.
WBS from Walmart, Penninton brand classic (least corn etc in it)
I put 1 40lb bag into 2 5 gal buckets, cleaning out all "floaters" and rinsing till "clean".  Then I fill up the bucket with the hottest tap I can put in it (my heater is set as high as It can go and will actually scold my hands)
let sit for 24 hours
after soaking, strain though strainers and cleaning as till water is clear.
put in a tub and lightly dried using a towel.  (due to my alt moisture Evaps fast so I need a more wet seed to start with). I don't have any pooling in my jars.

PC on and weight off, let sit till around 5psi then timed for 10min
add weight adn raise to 19/20 psi for 2 hours.
let cool and move jars into spawn room too cool down (spawn room is around 70º steady)
Quote:

Psicomvb said:
Are you using bags? Could your pressure gauge be defective?  How long are your PC cycles?






no bags, I've replaced my gauges due to someone saying that years ago, but nothing changed.


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Invisiblegive.more
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27302985 - 05/10/21 12:40 PM (3 years, 8 months ago)

Quote:

10kVisions said:


my last jar 1 out of 50 whats wrong here?







That jar looks like it might have yeast growing in it as well. I know my yeast from doing a lot of brewing and I've only had one jar grow it, so I'm not sure how correct I am on what I'm seeing.

It really might be ok, but that off color where the grains touch the glass makes me think yeast.


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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: give.more]
    #27302989 - 05/10/21 12:43 PM (3 years, 8 months ago)

Quote:

give.more said:
Quote:

10kVisions said:


my last jar 1 out of 50 whats wrong here?







That jar looks like it might have yeast growing in it as well. I know my yeast from doing a lot of brewing and I've only had one jar grow it, so I'm not sure how correct I am on what I'm seeing.

It really might be ok, but that off color where the grains touch the glass makes me think yeast.




someone else brought that up due to doing seed and substrate in teh same buckets to I bought 3 igloo coolers (also to keep my temps up longer for substrate) and still got the same result.  im not ruling it out of course... I just need to understand what im looking for and then of course why its happening.


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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27302990 - 05/10/21 12:45 PM (3 years, 8 months ago)

I took a photo of each side of that jar.  here you go.



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InvisiblePsicomb
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27302998 - 05/10/21 12:51 PM (3 years, 8 months ago)

Yeah I mean your jars look pretty good.  Hmm. I'm gonna sit on this issue and think for a little.


--------------------

When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand

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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: Psicomb]
    #27303001 - 05/10/21 12:53 PM (3 years, 8 months ago)

Quote:

Psicomvb said:
Yeah I mean your jars look pretty good.  Hmm. I'm gonna sit on this issue and think for a little.




thanks, I went though some of the TEK's in your signature and there 100% the way I do things... down to the letter basically. this is what is baffling me ahah.  I appreciate it.  Feel free to PM me, im better one on one with stuff honestly.

going to make agar now.


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InvisiblePsicomb
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Re: can an entire room be contaminated? [Re: 10kVisions]
    #27303006 - 05/10/21 12:56 PM (3 years, 8 months ago)

For sure dude, I'll message you.  Issues like this suck man, I feel your pain, a I montha after i switched to bags I tossed over 50 tubs before even getting a first flush cuz of PC issues, totally my fault, and I was focused on agar and grain prep and sterile work when really my shit just wasnt being PCd long enough.  I would recommend maybe adding an extra hour to your cycle and seeing if it changes anything.  2 hrs with jars is plenty from my experience but still, never know I guess


--------------------

When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand

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Invisible10kVisions
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Re: can an entire room be contaminated? [Re: Psicomb]
    #27303039 - 05/10/21 01:18 PM (3 years, 8 months ago)

"exposed grain is a potential contam vector"

I don't use casing layers.. ever. never did back when I was having super successful grows so don't think to do it now.  is there any truth to that statement?  could my whole probably be based around exposed grains getting contains without that layer?


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