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Invisiblenix21
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Registered: 05/29/18
Posts: 623
Swab broke
    #27301612 - 05/09/21 12:53 PM (3 years, 8 months ago)

I got wooden swab from a trade and its cracked just above tip, so i cant do normal swabbing as usual.. should i drop whole tip in agar plate or maybe use scalpel to cut some of cotton and bury it into dish?


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Offlinetiptrippy
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Registered: 09/09/20
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Re: Swab broke [Re: nix21]
    #27301616 - 05/09/21 01:00 PM (3 years, 8 months ago)

If you trust your sterile technique you can hold the swab in one hand using a pair of sterilized tweezers then with your other hand cut small pieces off and dunk them each into their own agar dish.

The simplest thing to do with the least contam vectors would be to just dunk the whole swab into a dish.

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Invisiblesandman420
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Registered: 06/17/04
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Re: Swab broke [Re: nix21]
    #27301617 - 05/09/21 01:01 PM (3 years, 8 months ago)

Tearing off is my preferred method. I see a lot of people are dipping it in sterile water first to prewash it and make it easier to tear.

I use a pair of needle holders to grasp the swab by the base when tearing of bits with tweezers.


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Invisiblesandman420
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Re: Swab broke [Re: sandman420]
    #27301621 - 05/09/21 01:04 PM (3 years, 8 months ago)

Personally I see a lot of bacteria when just dropping the whole swab in the dish. A lot of times the swab puffs up with mycelium but where the swab lays bacteria grows and prevents it from jumping out.

Antibacterial agar is a plus.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Invisiblenix21
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Registered: 05/29/18
Posts: 623
Re: Swab broke [Re: tiptrippy]
    #27301682 - 05/09/21 02:14 PM (3 years, 8 months ago)

I m starting again after year of pause, i think ill drop it and see how it goes... i have bunch of others prints so something will work out for sure :smile:
Thank you for advices


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