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Skizor1337
Trip King



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How are these agar plates doing?
#27301568 - 05/09/21 12:12 PM (3 years, 8 months ago) |
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Been a long time since I made any plates
Just curious how they look - Took the best pics I could and will update in a few days
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tiptrippy
The Mechanic



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Re: How are these agar plates doing? [Re: Skizor1337]
#27301590 - 05/09/21 12:37 PM (3 years, 8 months ago) |
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Hard to see still. What did you used to inoculate? Spore syringe or spore print?
Try taking a picture of it held up to a light shining through the back
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Skizor1337
Trip King



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Re: How are these agar plates doing? [Re: tiptrippy]
#27301710 - 05/09/21 02:44 PM (3 years, 8 months ago) |
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Quote:
tiptrippy said: Hard to see still. What did you used to inoculate? Spore syringe or spore print?
Try taking a picture of it held up to a light shining through the back
Spore syringe made from a print
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Skizor1337
Trip King



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Re: How are these agar plates doing? [Re: tiptrippy]
#27301766 - 05/09/21 03:30 PM (3 years, 8 months ago) |
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Quote:
tiptrippy said: Hard to see still. What did you used to inoculate? Spore syringe or spore print?
Try taking a picture of it held up to a light shining through the back
Here is a new pic
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QM33
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Re: How are these agar plates doing? [Re: Skizor1337]
#27301887 - 05/09/21 05:24 PM (3 years, 8 months ago) |
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For me,it lookscontaminated. I'm not familiar with all the names of the molds, but I've noticed one that almost looks like milk. I need to start getting those down. I could be myc but it looks too clear in the center,and looks kind of "puddle" like.
How old is it? And did you make the syringe? If so, how?
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monkey_accident
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Re: How are these agar plates doing? [Re: QM33]
#27301889 - 05/09/21 05:30 PM (3 years, 8 months ago) |
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I don't really see any myc on there, I'd try noccing some more plates in the meantime while these grow out some more. what nutes are in your agar?
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Hindsight
Mad Scientist


Registered: 01/24/21
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Your first pics were too hard for me to see, but the "new" pic is definitely bacterial. I don't see any mycelium there. I've never had luck with syringes onto agar. Ever. Not even once. If it's a syringe, it goes to a BRF cake that I then clone from. For spores, I always just drag my loop on a dry spore print and then drag it across the agar. It works very well.
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QM33
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Registered: 04/09/20
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Re: How are these agar plates doing? [Re: Hindsight]
#27301911 - 05/09/21 05:53 PM (3 years, 8 months ago) |
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I've had great luck with syringes I get from sporeworks, some grow out clean, apperantly, first run.
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Ashtray161
SettledNomad



Registered: 03/21/21
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Re: How are these agar plates doing? [Re: QM33]
#27301938 - 05/09/21 06:13 PM (3 years, 8 months ago) |
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Quote:
QM33 said: I've had great luck with syringes I get from s*****rks, some grow out clean, apperantly, first run.
same Almost<--- all clean on first go. Not always the strongest til the first transfer but ive mayb had 5% of the plates go bacterial or moldy from that vendors syringes.
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Edited by Ashtray161 (05/09/21 06:14 PM)
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Skizor1337
Trip King



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Re: How are these agar plates doing? [Re: QM33]
#27301946 - 05/09/21 06:19 PM (3 years, 8 months ago) |
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Quote:
QM33 said: For me,it lookscontaminated. I'm not familiar with all the names of the molds, but I've noticed one that almost looks like milk. I need to start getting those down. I could be myc but it looks too clear in the center,and looks kind of "puddle" like.
How old is it? And did you make the syringe? If so, how?
It's about a week old at this point
I made the syringe from a print I just made not that long ago.
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QM33
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Registered: 04/09/20
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Re: How are these agar plates doing? [Re: Skizor1337]
#27301956 - 05/09/21 06:29 PM (3 years, 8 months ago) |
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Ya probably bad if its that old. Unless it ape ha. That can grow super slow and in dense pockets, but that's about all I've seen that could even be a maybe.
I've never made a syringe, but check your sterile tek maybe. Make sure your water is sterilized.
-------------------- OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
there are also unknown unknowns.
With great privilege comes great responsibility.
 
Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
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monkey_accident
seraphim


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Re: How are these agar plates doing? [Re: QM33]
#27301985 - 05/09/21 06:53 PM (3 years, 8 months ago) |
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test the spores straight from the print?
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tiptrippy
The Mechanic



Registered: 09/09/20
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It's definitely bacterial. But if the spores end up germinating and you wait long enough they can outgrow the bacteria and you will eventually have some mycelium to transfer
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verum subsequentis
seeker of truth



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Re: How are these agar plates doing? [Re: tiptrippy]
#27302099 - 05/09/21 08:42 PM (3 years, 8 months ago) |
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Inthepit
Aum Mani Padme Hum


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Re: How are these agar plates doing? [Re: Skizor1337]
#27302578 - 05/10/21 07:34 AM (3 years, 8 months ago) |
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Did you take a clone from the shroom you printed?
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bigbrownbeaver
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Re: How are these agar plates doing? [Re: tiptrippy]
#27302588 - 05/10/21 07:47 AM (3 years, 8 months ago) |
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I completely agree with others this looks extremely bacterial and is probably just gonna be a loss.
I've never had luck with trying to let the myc from spores out grow bacteria. It always ends up hitching a ride with the myc when its contaminated from the get go for me. Even if I take the tiniest rice sized transfer from the very tip of the leading edge it doesnt work for me. At least with a regular 10glme 10g agar recipe that is.
I'm seeing some success by germinating questionable spores on a 4g lme 10g agar recipe. Seeems like the bacteria doesnt have enough nutrition to spread out quickly along with the mycelium when using lower nutes in plates. At least thats my observation so far. I'm no pro tho so take it with a grain of salt plz.
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Skizor1337
Trip King



Registered: 06/19/12
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Loc: USA
Last seen: 2 months, 6 days
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No worries I have to start over
Thanks everyone!
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