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fossilshark
DouchebagDonny


Registered: 08/05/20
Posts: 132
Last seen: 3 months, 3 days
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stasis storage
#27301204 - 05/09/21 07:21 AM (3 years, 10 days ago) |
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Hi, been lurking for a while, this is my first post.
I was wondering if anyone had any information on storing live cultures in stasis? from what i've read mycelium has been pulled out of stasis up to 2 decades after initial storage.
I want to preserve this culture as it is the second generation of an Enigma x Rusty Whyte cross that only a few of us are playing around with afaik.
What I did was boiled and pressure cooked some paper strips, then put them on the agar plates to colonize for a few weeks, then pressure cooked some distilled water and put them in pyrex petris.
anyone have any experience with stasis storage? things to be aware of/tips?
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trippleblack
Stranger

Registered: 12/01/19
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i'm all ears for good info too...
i do know that this method is unlikely to last comfortably anywhere close to a decade. i make new every slants every 6-9 months; only after loosing cultures which couldn't be recovered at the year mark. i believe most of us boil/hydrate wood sticks and put them in the culture tubes; not sure what paper will do in comparison.
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KristianF
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Registered: 04/16/17
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Last seen: 1 year, 7 months
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I put away some shiitake on a small wedge in distilled water in april -17 for a test. Stores it in a test tube, lid open slightly wraped with 3 layers of parafilm. Roomtemp all the time. Forgot about it...Put it on agar april -21 and it just tok of as if nothing happened...
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fossilshark
DouchebagDonny


Registered: 08/05/20
Posts: 132
Last seen: 3 months, 3 days
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this is very different from slants
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Quote:
fossilshark said: this is very different from slants
In what way? Slants are what you want for 2+ year storage anyway.
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fossilshark
DouchebagDonny


Registered: 08/05/20
Posts: 132
Last seen: 3 months, 3 days
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Storing the mycelium under stasis prevents the culture from changing, on slants the culture is still growing and changing albeit slowly.This can also be stored at room temperature. I chose this way of storing this culture because I want to preserve this strain for a very long time because of what it is.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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How are you going to put the mycelium in stasis without a deep freezer.
Cultures can be stored indefinitely on slants with glycerol at -80°c
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fossilshark
DouchebagDonny


Registered: 08/05/20
Posts: 132
Last seen: 3 months, 3 days
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cryostasis is something else lol. I believe this method is putting the myc in suspension. No oxygen and No nutrients makes the mycelium go into stasis.
I do not know if putting it in the fridge would be of any use, thats usually done with slants to slow down the metabolism but in this case there isn't really a metabolism to slow down.
I am just wondering if anyone who has any experience with this type of thing had any tips.
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trippleblack
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room temperature without nutes or air would be amazing, that means no senescence. would you need to suck all the water out if your not freezing.. freeze drying? since water has that dna degrading element in it of deuterium.
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MellowAmberGuitae
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Registered: 07/14/21
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You might be interested in this: https://www.shroomery.org/11586/Revival-of-saprotrophic-and-mycorrhizal-basidiomycete-cultures-after-20-years-in-cold-storage-in-sterile-water
You can store cultures in distilled water at room temperature (away from light). Lots of people have had success with it, you can search the forums.
Depriving mycelium of all nutes won't kill it but put it into hibernation(i know not the correct term), revival is easy, just put it back on agar.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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i really dont see the advantage over slants
ive used 7+ yr old slants with no issues at all using just a fridge. the myc isnt gonna be growing much in those temps, especially after colonizing all the surface, it doesnt really dig/. some species do store better than others though, all my pans die after a couple of years so gotta update them more regularly
i mean if you really want a liquid with no nutes, i dont see any advantage to what you are describing vs just using a liquid inoculant (or even a fully colonized LC)
also, i wouldnt assume that the culture changes more over a few years on a slant in the fridge (fully colonizing the half inch square of available surface) than it does over colonizing several square inches of agar in a week. maybe it does, but i definitely wouldnt assume so
the glycerol bod mentions sounds really cool, ive never looked into that or played with it but i would love to learn more about it. i cant imagine a fully colonized medium doing much genetic changing in -80c
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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