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Offlineshakezilla
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Need help with BRF jars pinning before colonized
    #27299462 - 05/07/21 11:10 PM (3 years, 8 months ago)

I have several BRF widemouth 1 pint jars that I prepared a little over a month ago now. I followed a few teks on this website. I PC'd them for 90 minutes at 15psi and inoculated them on April 4th with a B+ MSS. They appeared to begin to stall about a week and a half ago with not very much growth on them.

I tried to loosen the lids on two of the jars a few days ago and they have both grown significantly since then. The only problem is that they both have pins appearing on the sides of the jar now:


I'm not exactly sure what to do about these. Should I just let them keep going? Part of me thinks I should tighten the lid back up and the other part of me says to loosen it and see what happens. The other 10 jars are all still sitting around with the lids fully tight and they all still appear stalled out. I'm tempted to loosen all of them and see what happens.

I'm also considering making some more BRF jars and then tossing them into a ziplock bag with these partially colonized jars following another tek I saw on here. I was thinking that might give the FAE needed to colonize them all. Thoughts?

I have some other, smaller half-pint jars going with different MSS that are at various stages of growth, none of which appear to have stalled out luckily. But I also don't want to lose these larger pint jars just because they are larger.

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InvisibleAshtray161
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Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
Re: Need help with BRF jars pinning before colonized [Re: shakezilla]
    #27299463 - 05/07/21 11:14 PM (3 years, 8 months ago)

Stalling and early pinning are indictive of bacteria however those tall jars are also known for pinning early. Ill let someone with a bit more experience give u a suggestion on what to do with them cuz I dont wanna steer u wrong.


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OfflineFungi Rogers
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Registered: 12/31/20
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Re: Need help with BRF jars pinning before colonized [Re: Ashtray161]
    #27299471 - 05/07/21 11:25 PM (3 years, 8 months ago)

Do your jars have any means for GE? Holes in top? If not then having the lid completely tight is what’s stalling them. Your jars shouldn’t have taken a month to not colonize all the way.

There’s a couple things you could do.

Top fruit the jars.
Birth them and remove pins/not colonized spots.
Give them more GE and wait for them to finish.
Birth them remove uncolonized spots and mixing into a shoebox.

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OfflineHikeadellic
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Re: Need help with BRF jars pinning before colonized [Re: Ashtray161]
    #27299475 - 05/07/21 11:28 PM (3 years, 8 months ago)

Did you use a dry verm layer on top?

I would stop loosening the lids in the future

Honestly, it should be fine, let it do its thing for another few weeks, let it pin in the jar

Once the jar is fully colonized and ready to go to bulk, you will have a small early harvest to enjoy before the bulk puts out a nice harvest

That jar does look pretty big. It its 100% worth it to get a bunch of smaller jars. The OG pf tek calls for 1/4 pint (I think). This will have relatively quick and easy results. Half pint is really good too but it takes slightly longer so that means it has a slightly higher fail chance but it is still a very reliable method.

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OfflineHikeadellic
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Re: Need help with BRF jars pinning before colonized [Re: Fungi Rogers]
    #27299477 - 05/07/21 11:30 PM (3 years, 8 months ago)

Guy above me is right ^^

GE is very important

All the more reason to get some mason jars

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Offlineshakezilla
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Re: Need help with BRF jars pinning before colonized [Re: Fungi Rogers]
    #27299513 - 05/08/21 12:28 AM (3 years, 8 months ago)

Yeah I have a 1/4" hole on top with a filter over it. I also have a dry verm layer. And they are mason jars. So they are getting some GE even when the lids are fully tight. Just not as much as when I loosened them.

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Offlineshakezilla
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Registered: 08/19/12
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Re: Need help with BRF jars pinning before colonized [Re: shakezilla]
    #27301812 - 05/09/21 04:05 PM (3 years, 8 months ago)

These pins are getting big so quickly!


Can I spawn this in a SAB and clone the pins? That way I can at least get something going on agar. I'm thinking of cloning the pins and then throwing the rest of the cake into a ziplock bag to see if it does anything. But it's not colonized enough to stand on it's own.

Also here's a picture of the lids so you can see that they are getting GE without loosening the lids

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InvisibleGoatrider
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Re: Need help with BRF jars pinning before colonized [Re: shakezilla]
    #27302276 - 05/09/21 11:52 PM (3 years, 8 months ago)

Is just the bottom uncolonized?
If so i'd get rid of the uncolonized parts,
crumble the cake up and spawn to a shoebox with some coir.
No need to do this in a sab.
Always a good idea to clone, here in a sab of course :wink:

              :cookiemonster:


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