|
Bcuzz
Stranger
Registered: 05/07/21
Posts: 97
Last seen: 2 years, 4 months
|
Is it contamination or can I sleep peacefully?
#27298976 - 05/07/21 05:01 PM (3 years, 8 months ago) |
|
|
Good day, I have been a silent reader of shroomery for about 1 year now and have found pretty much everything before posting anything because logically it was just not necessary.
Well, in the end I still have a question and it's about contamination. I have no idea what it is exactly, which is why I would like to ask the experts for advice.In the end, no matter how much you read, when it happens to you, you don't really know anymore.
    
  I know some of these Pictures are crap but i couldnt do it better. I assume it is contamination, my question is where does it come from? I use a laminar flow hood and still have contamination in the glass. You can see from the agar that the plate is clean.
Edited by Bcuzz (05/07/21 05:04 PM)
|
Rot
Worlds best trichoderma farmer


Registered: 11/09/18
Posts: 908
Last seen: 3 years, 8 months
|
Re: Is it contamination or can I sleep peacefully? [Re: Bcuzz]
#27298985 - 05/07/21 05:07 PM (3 years, 8 months ago) |
|
|
If it's not on the agar then it probably happened when you dropped your agar to your grains. When doing agar to grain in my experience that is the time most things are exposed and can go wrong. At least for me. I have a hard time being super sterile when trying to hold everything and be quick. Flow hoods aren't 100% safe but they are really good. Even with a flow hood you did wipe everything down with iso right?
Edit: Did you do more than one jar? If so are they all like this? Do you have agar saved to redo them?
Edited by Rot (05/07/21 05:08 PM)
|
Bcuzz
Stranger
Registered: 05/07/21
Posts: 97
Last seen: 2 years, 4 months
|
Re: Is it contamination or can I sleep peacefully? [Re: Rot]
#27298993 - 05/07/21 05:12 PM (3 years, 8 months ago) |
|
|
I thoroughly disinfect the work surface beforehand, yes, just like my tools. To be honest, I leave the lids and Petri dishes almost untouched.
I also strongly believe that it was caused in the transfer, but I am also convinced that I used the pressure cooker incorrectly. I did not let it cool down, but let the air escape immediately.
|
Rot
Worlds best trichoderma farmer


Registered: 11/09/18
Posts: 908
Last seen: 3 years, 8 months
|
Re: Is it contamination or can I sleep peacefully? [Re: Bcuzz]
#27299008 - 05/07/21 05:21 PM (3 years, 8 months ago) |
|
|
Quote:
Bcuzz said: I thoroughly disinfect the work surface beforehand, yes, just like my tools. To be honest, I leave the lids and Petri dishes almost untouched.
I also strongly believe that it was caused in the transfer, but I am also convinced that I used the pressure cooker incorrectly. I did not let it cool down, but let the air escape immediately.
Oh that's also very possible if the grains weren't pc'd right. Not letting it cool down long enough isn't an issue. As long as you don't drop agar on lava hot grains. Did you PC for 90 minutes at 15 psi?
edit: also, I recently made 18 jars and one of them looked exactly like that one. I left it alone for a few more days and it turned jet black. So hold onto it for a few more days.
Edited by Rot (05/07/21 05:22 PM)
|
Bcuzz
Stranger
Registered: 05/07/21
Posts: 97
Last seen: 2 years, 4 months
|
Re: Is it contamination or can I sleep peacefully? [Re: Rot]
#27299025 - 05/07/21 05:30 PM (3 years, 8 months ago) |
|
|
I assume that I have sterilised it correctly. At 90 minutes with 15 psi and before that let the air escape for 10 minutes. Each picture is of a different glass. I agree with you that I also assumed with 1-2 glasses that I could throw them away.
|
LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 2,329
|
Re: Is it contamination or can I sleep peacefully? [Re: Bcuzz]
#27299075 - 05/07/21 05:59 PM (3 years, 8 months ago) |
|
|

Is that not trich on the left corner of the transfer wedge?
-------------------- Don't follow leaders
|
Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,788
Loc: A thought
|
Re: Is it contamination or can I sleep peacefully? [Re: LadysKnight]
#27299079 - 05/07/21 06:02 PM (3 years, 8 months ago) |
|
|
Yeah man,what is that?
-------------------- * Smarts videos
* Planet of the APES
 
I'm a fungal white supremacist.
|
LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 2,329
|
Re: Is it contamination or can I sleep peacefully? [Re: Smartattack]
#27299082 - 05/07/21 06:05 PM (3 years, 8 months ago) |
|
|
-------------------- Don't follow leaders
|
Bcuzz
Stranger
Registered: 05/07/21
Posts: 97
Last seen: 2 years, 4 months
|
Re: Is it contamination or can I sleep peacefully? [Re: LadysKnight]
#27299111 - 05/07/21 06:25 PM (3 years, 8 months ago) |
|
|
My bad, its just ash from mycelium. I had some mycelium leftovers on my blade and it burned everything after i sterilized the blade
|
LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 2,329
|
Re: Is it contamination or can I sleep peacefully? [Re: Bcuzz]
#27299128 - 05/07/21 06:31 PM (3 years, 8 months ago) |
|
|
Ok then. Otherwise,the plates are too overgrown to see much. But that second plate appears to have mold in the bottom-right.
-------------------- Don't follow leaders
|
Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
|
Re: Is it contamination or can I sleep peacefully? [Re: Bcuzz]
#27299269 - 05/07/21 08:05 PM (3 years, 8 months ago) |
|
|
Quote:
Bcuzz said: I did not let it cool down, but let the air escape immediately.
cant that make it or the jars explode? lol
--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312
Edited by Ashtray161 (05/07/21 08:06 PM)
|
Bcuzz
Stranger
Registered: 05/07/21
Posts: 97
Last seen: 2 years, 4 months
|
Re: Is it contamination or can I sleep peacefully? [Re: Ashtray161]
#27301742 - 05/09/21 03:17 PM (3 years, 8 months ago) |
|
|
Well im glad they didnt explode, so i throw them away and used just the half of my jars. i hope it will turn out fine
|
Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
|
Re: Is it contamination or can I sleep peacefully? [Re: Bcuzz]
#27301744 - 05/09/21 03:18 PM (3 years, 8 months ago) |
|
|
idk that for sure i just remember reading something about not removing the air early
--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312
|
Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,801
Loc: Germany
|
Re: Is it contamination or can I sleep peacefully? [Re: Ashtray161]
#27302258 - 05/09/21 11:28 PM (3 years, 8 months ago) |
|
|
I advise to always let the pc release pressure on it's own. Not only a contam vector, also messing up your moisture content. I always test with my wrist carefully touching the pc outside, by the time i won't get burned, i take the jars out, screw lids on tight and shake to redistribute moisture. Wait a few hours to cool completely down, then ready to go.
--------------------
|
In Lakech
Ala Kin


Registered: 10/09/18
Posts: 131
Loc: this
|
Re: Is it contamination or can I sleep peacefully? [Re: Goatrider]
#27302320 - 05/10/21 01:45 AM (3 years, 8 months ago) |
|
|
All I see is some shading in the jars. Is there a color you are seeing? Give it a couple of days and see if something more appears. Could be nothing.
Upon closer inspection it looks like something darker is in there. If you see blue growth it could be Penicillium. If that's the case toss the jars and start over. It's a common problem. The air is full of shit. No avoiding it. Sanitize all surfaces in your work room, including the floor and run your filter for a half hour before doing your transfers.
-------------------- I will forever miss spirit surfing the multicolored waves of Jerry's guitar riffs!
Edited by In Lakech (05/10/21 01:53 AM)
|
Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,801
Loc: Germany
|
Re: Is it contamination or can I sleep peacefully? [Re: In Lakech]
#27302327 - 05/10/21 01:53 AM (3 years, 8 months ago) |
|
|
For sure there`s something going on, as you can see spikey mycelium. Not that this would be the end of the road, but i really don`t like the colours in there. Quite sure that will mold away.
--------------------
|
Bcuzz
Stranger
Registered: 05/07/21
Posts: 97
Last seen: 2 years, 4 months
|
Re: Is it contamination or can I sleep peacefully? [Re: Goatrider]
#27302549 - 05/10/21 07:05 AM (3 years, 8 months ago) |
|
|
i threw them away already, its kind of sad to fight with problems like this even with a laminar flow hood, i thought i gained already enough experience but i have still the same problems
|
In Lakech
Ala Kin


Registered: 10/09/18
Posts: 131
Loc: this
|
Re: Is it contamination or can I sleep peacefully? [Re: Bcuzz]
#27302911 - 05/10/21 11:44 AM (3 years, 8 months ago) |
|
|
Sometimes it's just what's in the air where you live. For a while I lived across from a micro brewery and the air was so full of yeast my grain jars all smelled fermented. Hang in there.
-------------------- I will forever miss spirit surfing the multicolored waves of Jerry's guitar riffs!
|
Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,801
Loc: Germany
|
Re: Is it contamination or can I sleep peacefully? [Re: In Lakech]
#27303131 - 05/10/21 02:49 PM (3 years, 8 months ago) |
|
|
Maybe try a little project in a sab instead of using the flowhood. Possibly there`s something off. Just try again, and keep it as simple as possible to narrow contam vectors. Good luck mate 
--------------------
|
Bcuzz
Stranger
Registered: 05/07/21
Posts: 97
Last seen: 2 years, 4 months
|
Re: Is it contamination or can I sleep peacefully? [Re: Goatrider]
#27303195 - 05/10/21 03:58 PM (3 years, 8 months ago) |
|
|
i used a sab and it didnt work out thats why i switched to a flowhood. but well maybe its the location, im living in an old house and the windows are not 100% sealed anymore
|
|