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greggabott2
Registered: 10/26/20
Posts: 40
Loc: Canada
Last seen: 1 year, 4 months
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How’s my first LC looking?
#27297818 - 05/06/21 10:29 PM (2 years, 4 months ago) |
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Howdy everyone. Recently tried my hand at doing some LIquid culture. I used polyfill for the lid and tried my best not to let the water touch it much when shaking (more like twisting it). How’s this looking? I know it’s difficult to tell contamination but would you say it’s ready to start testing? Thanks.
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: How’s my first LC looking? [Re: greggabott2]
#27297843 - 05/06/21 10:45 PM (2 years, 4 months ago) |
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from what i understand you cannot tell good LC by looking at it, like ever.
Test it on agar
My first LC I was about to toss cuz it looked crazy, tested it on agar and its not only clean apparently but has awesomely aggressive quick growing myc so u rly dont knw. My 2nd I thought was fine and was nothing BUT bacteria and mold spores
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
Edited by Ashtray161 (05/06/21 10:46 PM)
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kanemush
Grumpy

Registered: 12/07/17
Posts: 1,502
Last seen: 5 months, 3 days
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Re: How’s my first LC looking? [Re: Ashtray161]
#27297926 - 05/07/21 12:06 AM (2 years, 4 months ago) |
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PitcherCrab
Crescent Fresh



Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 9 months, 20 days
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Re: How’s my first LC looking? [Re: kanemush]
#27297934 - 05/07/21 12:14 AM (2 years, 4 months ago) |
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Yeah man, exactly what these two said. Looks good as in its not cloudy, but make sure to test it on agar before doing anything with it. I recently tried LCs again and ended up making one that was pure cobweb mold and another that I thought was RW ended up being contamed with yellow oysters. Kinda funny actually, but a bummer when they started to fruit. I may try them again, but for now I’m sticking with agar plates, just too many ways to mess up LC IME.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
Edited by PitcherCrab (05/07/21 12:18 AM)
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: How’s my first LC looking? [Re: PitcherCrab]
#27297977 - 05/07/21 01:26 AM (2 years, 4 months ago) |
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Quote:
PitcherCrab said: Yeah man, exactly what these two said. Looks good as in its not cloudy, but make sure to test it on agar before doing anything with it. I recently tried LCs again and ended up making one that was pure cobweb mold and another that I thought was RW ended up being contamed with yellow oysters. Kinda funny actually, but a bummer when they started to fruit. I may try them again, but for now I’m sticking with agar plates, just too many ways to mess up LC IME.
How tf did that happen? xD You must be growing yellow oysters too that spored and contamed that way right? Like yellow oyster spores arent just chillin like trich are they? xD Hearing a TC also struggles w LC makes my newb ass not feel as bad and kinda proud that ive had a 50% success rate so far xD The craziest thing is it was from a MSS too. Total beginners luck
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
Edited by Ashtray161 (05/07/21 01:26 AM)
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PitcherCrab
Crescent Fresh



Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 9 months, 20 days
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Re: How’s my first LC looking? [Re: Ashtray161]
#27298374 - 05/07/21 09:09 AM (2 years, 4 months ago) |
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The yellow oyster contamination was fully my fault. I was growing a ton last Summer so my grow space was full of their spore dumps – I was pulling 20+ 5-6lb clusters per week. I started noticing a lot of rogue spore germinations on my plates and when I went to make LC some must have gotten in. For the cobweb mold, I think that was the one I tried making from MS, so that makes more sense. LCs are just hard, you have to be extra careful and even then you can mess it up.
That's awesome you got a clean one from MMS! It's a crap shoot, but the fact it's difficult to see what's going on in the LC makes me very wary.
I also need a better lab space lol, my flow hood is just in my garage so I'm not surprised I get some issues from time to time.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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