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InvisibleGuerrilla
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Registered: 01/30/21
Posts: 3,179
Loc: United Kingdom Flag
Questions about LC vacuum ?
    #27295211 - 05/05/21 08:19 AM (3 years, 8 months ago)

Hi,

Making LCs tomorrow and just wondering if I need to do anything like partially loosen the lid in PC and wrap with foil to avoid a vacuum when I open the lid up to drop an agar wedge in. I have a syringe filter on it.

And should I be injecting flame sterilised air equal to the amount of liquid being drawn each time through a SHIP?


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,266
Last seen: 1 month, 22 days
Trusted Cultivator
Re: Questions about LC vacuum ? [Re: Guerrilla]
    #27295220 - 05/05/21 08:24 AM (3 years, 8 months ago)

No your Syringe filter will allow it to breath.

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InvisibleGuerrilla
Original Asshole


Registered: 01/30/21
Posts: 3,179
Loc: United Kingdom Flag
Re: Questions about LC vacuum ? [Re: Sockadin]
    #27295282 - 05/05/21 09:04 AM (3 years, 8 months ago)

Yeah I figured that’d be the case, cheers.

What about injecting air before drawing out liquid?


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Being pissed on does not make you a real man.

...OR DOES IT?

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Invisiblesandman420
Saint PP
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Registered: 06/17/04
Posts: 5,387
Trusted Cultivator
Re: Questions about LC vacuum ? [Re: Guerrilla]
    #27295287 - 05/05/21 09:12 AM (3 years, 8 months ago)

no  need with a filter on lid


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