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Invisiblesandman420
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Re: Scalpel not turning red [Re: ninja cat 09] * 1
    #27309799 - 05/15/21 12:19 PM (3 years, 7 months ago)

Very cool ninja cat! If the blade is getting hot enough to sizzle that is plenty. No need for the red commie blade. :thumbup:

More people need to do dry practice runs with their gear before they get a beloved culture that they botch hehe.

Try some more and bring that transfer size down to 1/4 that size IMO.





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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

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Edited by sandman420 (05/15/21 12:20 PM)

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Invisibleninja cat 09
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Registered: 10/11/09
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Re: Scalpel not turning red [Re: sandman420]
    #27309962 - 05/15/21 03:19 PM (3 years, 7 months ago)

Thanks for the tips sandman!

I read I'm not sure if here  or in Growing Gourmet and Medicinal Mushrooms that one should. I'm feeling impatient to get my hands dirty so to say, but I've already figured out a couple issues with my sterile technique by doing that which would have botched the 11 year old cultures from slants I'm trying to preserve!


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Invisibleninja cat 09
A paranoid android
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Registered: 10/11/09
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Loc: Mexico Flag
Re: Scalpel not turning red [Re: ninja cat 09]
    #27312801 - 05/17/21 05:01 PM (3 years, 7 months ago)

So, another update because I got more contams but none from the cuts:



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Invisiblesandman420
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Registered: 06/17/04
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Re: Scalpel not turning red [Re: ninja cat 09]
    #27312831 - 05/17/21 05:37 PM (3 years, 7 months ago)

Hopefully you can identify where the contams are coming from? Looks like they are growing inside the agar?


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (05/17/21 05:38 PM)

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Invisibleninja cat 09
A paranoid android
Male User Gallery


Registered: 10/11/09
Posts: 4,192
Loc: Mexico Flag
Re: Scalpel not turning red [Re: sandman420]
    #27313540 - 05/18/21 09:31 AM (3 years, 7 months ago)

Most of them are from that splashing issue I mentioned in the last post, another seems to be coming from the edge of the petri somehow, but so far nothing from the blade. Thankfully the receiving transfer plate, one of the red hot and one of the 10 second ones have survived.


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