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ninja cat 09
A paranoid android



Registered: 10/11/09
Posts: 4,192
Loc: Mexico
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Re: Scalpel not turning red [Re: sandman420]
#27295465 - 05/05/21 12:21 PM (3 years, 8 months ago) |
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My previous mention was for plastic petris, some guy on mycotek has been apparently reusing his for over a year. Another poster suggested using a towel they put in the PC to dry them off instead of using hot water to clean off the bleach. I'm a cheap bastard, so I'll be giving that a try Another user on that same thread apparently oven sterilizes them (2 hours, 150 C)! 
EDIT: Just put a few in the oven now.
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Edited by ninja cat 09 (05/05/21 12:27 PM)
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Inthepit
Aum Mani Padme Hum


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 1 day
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Wow, I can't see any of that would work. I can't wait to see the responses to that!
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ninja cat 09
A paranoid android



Registered: 10/11/09
Posts: 4,192
Loc: Mexico
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Re: Scalpel not turning red [Re: Inthepit]
#27295477 - 05/05/21 12:35 PM (3 years, 8 months ago) |
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It's not the first time I've seen it mentioned online, and here's a paper on using bleach to sterilize plastic petri dishes with good results and no inhibition of fungi growth by traces of NaOCl.
The oven thing I'm more convinced was to convince some idiot like me to put plastic in the oven, but I'll report back in a few hours to tell you if my house burnt down
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,387
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yea I dont know about that haha...you are wild
But I just looked it up and polystyrene has a glass transition temp of 212f./100c..
edit I put wrong number lol
Edited by sandman420 (05/05/21 12:43 PM)
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Shroombie2
Stranger
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Posts: 190
Last seen: 4 months, 4 days
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Re: Scalpel not turning red [Re: sandman420]
#27295531 - 05/05/21 01:31 PM (3 years, 8 months ago) |
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I use these small sized filter boxes for agar. I don't need to pour my agar into something after sterilization but I can't fit more than 4 of these in my PC at a time.
My vendor used to have even smaller size which was only shorter but had the same base, it's a shame they don't have them anymore, they were even better than these.
Edited by Shroombie2 (05/06/21 03:12 AM)
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woofwoof
such mushrooms!



Registered: 01/04/19
Posts: 1,127
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Re: Scalpel not turning red [Re: Shroombie2]
#27295551 - 05/05/21 01:50 PM (3 years, 8 months ago) |
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I just use my kitchen stove (gas). I turn it on high then start my sab setup and put my agar and stuff in
Then I stand up and walk over to my stove and flame my scalpel between plates and never had an issue. I just leave it over the flame for about 25 to 30 seconds till its red hot, then walk over to my table, sit down, cool blade in receiving dish and do my xfers
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the_chosen_one
So Called Sage


Registered: 09/11/06
Posts: 3,950
Loc: 1984
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Re: Scalpel not turning red [Re: sandman420]
#27295576 - 05/05/21 02:05 PM (3 years, 8 months ago) |
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Quote:
sandman420 said: whatup Taco!
Hey Sandy! Good to see you. Just came out of the lurk to yell at some kids on the lawn. Next week I think I'll step it up a bit and record speeders going past my house on my cell phone.
-------------------- "Luck favors the observant." - Workman
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ninja cat 09
A paranoid android



Registered: 10/11/09
Posts: 4,192
Loc: Mexico
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Well, it worked, sorta. I've gotten myself a very sterile paper weight:

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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,387
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Ah yes, reedy to pour
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Shroombie2
Stranger
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Re: Scalpel not turning red [Re: sandman420]
#27296508 - 05/06/21 03:11 AM (3 years, 8 months ago) |
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It's really weird that there are still no petri dishes made of heatable plastic. Like the ones I use, but they are made for BRF cakes and not agar. I wish they started to make them shorter again. It's also weird that these stuff are not available to you in the US (I'm assuming?) since they are very light to transport (I understand Presto PC is not available in EU) and really makes this hobby so much easier.
Edited by Shroombie2 (05/06/21 03:12 AM)
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myc_ousin_vinny
Keeping_It_Real



Registered: 04/29/20
Posts: 1,417
Loc: United States
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Re: Scalpel not turning red [Re: Shroombie2] 1
#27296543 - 05/06/21 04:57 AM (3 years, 8 months ago) |
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Quote:
Shroombie2 said: It's really weird that there are still no petri dishes made of heatable plastic. Like the ones I use, but they are made for BRF cakes and not agar. I wish they started to make them shorter again. It's also weird that these stuff are not available to you in the US (I'm assuming?) since they are very light to transport (I understand Presto PC is not available in EU) and really makes this hobby so much easier.
Not petri dishes per se but these containers rock... no mods. Alien's Holy Grail No Pour Agar Unomodified Containers
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Shroombie2
Stranger
Registered: 12/28/18
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Those seem great too!
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 12,109
Loc: South Africa
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Re: Scalpel not turning red [Re: Shroombie2]
#27296663 - 05/06/21 07:43 AM (3 years, 8 months ago) |
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I love these, would prefer them clearer but they are so cheap and work like a charm. Pp 5
Edited by DERRAYLD (05/06/21 07:43 AM)
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,387
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Re: Scalpel not turning red [Re: DERRAYLD]
#27296666 - 05/06/21 07:45 AM (3 years, 8 months ago) |
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PP whats that now?
Oh yea just PP.
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 12,109
Loc: South Africa
Last seen: 47 minutes, 11 seconds
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Re: Scalpel not turning red [Re: sandman420]
#27296670 - 05/06/21 07:47 AM (3 years, 8 months ago) |
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Quote:
sandman420 said: PP whats that now?
I made sure to keep the space between the 5 and the pp to not upset you, I realize now it's just pp but then how would I differentiate between s and 5?
Edited by DERRAYLD (05/06/21 07:48 AM)
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,387
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Re: Scalpel not turning red [Re: DERRAYLD]
#27296677 - 05/06/21 07:49 AM (3 years, 8 months ago) |
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I was called by god to change the ways of PP...what a lame calling.
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 12,109
Loc: South Africa
Last seen: 47 minutes, 11 seconds
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Re: Scalpel not turning red [Re: sandman420]
#27296683 - 05/06/21 07:51 AM (3 years, 8 months ago) |
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Quote:
sandman420 said: I was called by god to change the ways of PP...what a lame calling.
As long as you put the pp first.... always put the pp first
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ninja cat 09
A paranoid android



Registered: 10/11/09
Posts: 4,192
Loc: Mexico
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Quote:
myc_ousin_vinny said: Not petri dishes per se but these containers rock... no mods. Alien's Holy Grail No Pour Agar Unomodified Containers
I completely forgot about those! If I can't get the bleach to sterilize the petris enough, I'll look into getting some in Mexico!
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Shroombie2
Stranger
Registered: 12/28/18
Posts: 190
Last seen: 4 months, 4 days
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Found a bigger torch for $30, although still mostly butane (some perscentage propane) it has much larger flame and the same scalpel turn all red within 3 seconds. And it has a continuous mode Now this part of mycology is going to become a joy
Edited by Shroombie2 (05/09/21 10:36 AM)
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ninja cat 09
A paranoid android



Registered: 10/11/09
Posts: 4,192
Loc: Mexico
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Re: Scalpel not turning red [Re: Shroombie2]
#27309744 - 05/15/21 11:33 AM (3 years, 7 months ago) |
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So, I put my money where my mouth is and did some tests on the 13th of May, I had a pair of control plates where I was able to get (a thinner) scalpel red hot and did a few cuts on two different dishes, flaming between dishes:
 
Then, using a thicker scalpel which doesn't get red as easily, I flamed it for 10 seconds and did the same, it also sizzled when touching the agar the first time:
 
Then, I did some transfer practice, using the blade from the control plates, but only flaming it for 10 seconds each transfer, you can see some contams, but it's because I sprayed too much soap water on the top of my SAB and a drop fell and splashed. No contams so far on any of the cuts or transfers (the spots on the receiving plate are sawdust)
  
I wanted to wait longer, but since I saw contams which didn't originate from the blade, so I posted now before it became more difficult to differentiate. I'll update in a few days. Finally, the blue plates (except for the plate which received the transfer) are PSYA (Potato, Sugar, Yeast, Agar) plates without the potato because I'm a doofus and I forgot, the green ones are a premix of PDA which contams grow fine on and the dish that received the transfers is PSYA + sawdust.
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