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OfflinePBJ710
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Re: Scalpel not turning red [Re: ninja cat 09]
    #27294162 - 05/04/21 02:58 PM (3 years, 8 months ago)

+1 for real torch.  This is one of my favorites - takes 3-4 seconds for a #11 blade in a scalpel handle.


Edited by PBJ710 (05/04/21 05:30 PM)

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OfflineShroombie2
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Re: Scalpel not turning red [Re: PBJ710]
    #27294196 - 05/04/21 03:34 PM (3 years, 8 months ago)

I'm getting one of those torches with replacable bottles. I see some bottle has more propan than others. It should be better than the torch I have now and also I don't need to refill it so maybe it lasts longer. Also getting denatured alcohol for my lamp. The torch is probably still hotter but need to see if denatured alcohol is better than the liquid I have now.

I can't find a propane torch for under $100 without shipping costs. Also, the stores I've been to all have butane and these replacable bottles but no propane.

Edited by Shroombie2 (05/04/21 03:35 PM)

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OfflinePBJ710
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Re: Scalpel not turning red [Re: Shroombie2]
    #27294403 - 05/04/21 05:38 PM (3 years, 8 months ago)

Those are $50 from the manufacturer - sorry I said only propane earlier as either propane or butane will work fine.  The more focused the flame, the easier it will be to keep your handle cool.

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OfflineInthepit
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Re: Scalpel not turning red [Re: PBJ710]
    #27294491 - 05/04/21 06:59 PM (3 years, 8 months ago)

   
Pics of my torch


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

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InvisibleAshtray161
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Re: Scalpel not turning red [Re: Inthepit]
    #27294528 - 05/04/21 07:19 PM (3 years, 8 months ago)

Quote:

Inthepit said:
   
Pics of my torch




ooo thats a good idea, having the little hole for ur flame sterilization. imma do that


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Invisiblethe_chosen_one
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Re: Scalpel not turning red [Re: PBJ710] * 2
    #27294538 - 05/04/21 07:24 PM (3 years, 8 months ago)

:oldman: I've been doing this for over thirty years and never once.. ever, have I made a scalpel or needle glow red. I'm a mycologist not a blacksmith! No need for the Acme Anvil and billows with agar. A pressure cooker / autoclave go better with
lab coats anyways.
Seriously though. If you sterilize all of your tools in a PC and get them quickly and cleanly to the work area they only require minimal flame to eliminate static klingons.


--------------------
"Luck favors the observant." - Workman


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OfflineShroombie2
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Re: Scalpel not turning red [Re: the_chosen_one]
    #27294952 - 05/05/21 02:45 AM (3 years, 8 months ago)

I acutally don't think the reason for my recent failures are because of the scalpel not being sterile. In fact, I believe you can get away with desinfecting the scalpel, you can desinfect petri dishes after use (bleach works, ISO doesn't) and get great agar cultures, why can't the scalpel also just be desinfected?

It's just I waste so much time with the torch that it kills all the fun. I just want to focus on technique instead I have to battle my torch most of the time everytime. Also, torching should be fun when it works easily, it's fire!

I'm getting a bigger torch with replacable containers. I think the containers have a standard valve and I can get containers with 25% porpane for $5. There is no 100% propane as I understand it and I can't find a propane refiller like the butane refiller I have.

Edited by Shroombie2 (05/05/21 02:51 AM)

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OfflineInthepit
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Re: Scalpel not turning red [Re: Ashtray161]
    #27295045 - 05/05/21 05:11 AM (3 years, 8 months ago)

Quote:

Ashtray161 said:
Quote:

Inthepit said:

Pics of my torch




ooo thats a good idea, having the little hole for ur flame sterilization. imma do that




Ok, I had second thoughts about posting those pics, but it's all I had of this great torch.

                               

The thing is I haven't finished testing this idea.
I had to get my petri contams down to <1%. So here's a link to the discussion about it.

Side hole in SAB for a torch - danger!  Wow, 6 months ago...
Let the flamer beware!  :rofl:

Edited by Inthepit (05/05/21 05:41 AM)

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OfflineShroombie2
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Re: Scalpel not turning red [Re: Inthepit]
    #27295070 - 05/05/21 05:39 AM (3 years, 8 months ago)

Cool. Is that 100% propane och a mixture of butane and propane?

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OfflineInthepit
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Re: Scalpel not turning red [Re: Shroombie2]
    #27295082 - 05/05/21 05:51 AM (3 years, 8 months ago)

Well the tank is labeled P PROPANE and it reads,
"Use only with torches designed for propane gas."

Quote:

they only require minimal flame to eliminate static klingons.
thanks chosen_one!



Edited by Inthepit (05/05/21 05:57 AM)

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Invisiblesandman420
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Re: Scalpel not turning red [Re: Inthepit]
    #27295085 - 05/05/21 05:59 AM (3 years, 8 months ago)

I prefer the green camping canisters for my propane torch because they are shorter and wider = more stable on the table.

whatup Taco!


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

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OfflineShroombie2
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Re: Scalpel not turning red [Re: Inthepit]
    #27295092 - 05/05/21 06:05 AM (3 years, 8 months ago)

I see. I think it's different where I live. I can't find anything with 100% propan, only mixes. The highest percentage of propane I've seen is 30. Otherwise they have american valves.

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OfflineInthepit
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Re: Scalpel not turning red [Re: sandman420]
    #27295095 - 05/05/21 06:09 AM (3 years, 8 months ago)

Quote:

sandman420 said:
I prefer the green camping canisters for my propane torch because they are shorter and wider = more stable on the table.
whatup Taco!



Ya, I think was yours I saw, very stable with a small footprint!

whatup Taco!

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OfflineTheBoJim
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Re: Scalpel not turning red [Re: Inthepit]
    #27295169 - 05/05/21 07:31 AM (3 years, 8 months ago)

I have seen videos of mycologists working in real labs passing the blade over a flame for just a few seconds without it becoming red. Maybe they know something.


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Invisibleninja cat 09
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Re: Scalpel not turning red [Re: the_chosen_one]
    #27295225 - 05/05/21 08:28 AM (3 years, 8 months ago)

Quote:

Shroombie2 said:
, you can desinfect petri dishes after use (bleach works, ISO doesn't) and get great agar cultures, why can't the scalpel also just be desinfected?



You probably could, but you'd spend a lot more time waiting for it to dry than you would just flaming it. Chlorine drops on petri dishes probably aren't the best. Many people here have tried just using ISO and it doesn't work.

As far as petris go, I read on some random website that you should use chlorine, then alcohol, I assume because they'll dry quicker like that. Maybe you could try that and see your levels of success, but I think it's a lot more hassle than it's worth.

Quote:

TheBoJim said:
I have seen videos of mycologists working in real labs passing the blade over a flame for just a few seconds without it becoming red. Maybe they know something.



Quote:

the_chosen_one said:
Seriously though. If you sterilize all of your tools in a PC and get them quickly and cleanly to the work area they only require minimal flame to eliminate static klingons.




That's my train of thought as well, I think it's important for needles to get red hot because you need the inside to get sterile too, plus it's extremely thin material, but in the case of scalpels, an ethanol flame is 1920 degrees Celsius, your average pressure cooker at 15 PSI gets up to around 120 degrees C, so getting it red hot seems like overkill to me. Especially since you only need to sterilize the exterior of the blade.


--------------------
             :crazykitty:

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Invisiblesandman420
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Re: Scalpel not turning red [Re: ninja cat 09]
    #27295234 - 05/05/21 08:33 AM (3 years, 8 months ago)

Microbes die at a much lower temp than 1400 degrees



--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (05/05/21 08:34 AM)

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Invisibleninja cat 09
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Re: Scalpel not turning red [Re: sandman420]
    #27295279 - 05/05/21 09:00 AM (3 years, 8 months ago)

EDIT: :lol: I read higher, not lower.

Well, the CDC seems to disagree with that (see gravity displacement, I.E. pressure cookers). I understand it's a time-heat relationship, but even for dry heat the temperatures are pretty low compared to a direct flame. It doesn't seem unreasonable to me that a few seconds at 1920 C would effectively sterilize everything on the surface, especially if we've put our tools/blades in the PC first.

If you have a source which states otherwise I'd love to see it :smile:

Plus, we're not going for full sterilization, otherwise SABs would not be effective. Either way, I'll follow through and test my hypothesis once my recently poured petris have a chance to show any possible contaminants and post a thread with the results.


--------------------
             :crazykitty:

Edited by ninja cat 09 (05/05/21 09:00 AM)

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Invisiblesandman420
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Re: Scalpel not turning red [Re: ninja cat 09] * 1
    #27295285 - 05/05/21 09:09 AM (3 years, 8 months ago)

I sterilize glass petris in a oven at 350F lol works fine.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineInthepit
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Re: Scalpel not turning red [Re: sandman420]
    #27295362 - 05/05/21 10:36 AM (3 years, 8 months ago)

:whathesaid:  me too!  Corning Soda Lime Silica 100x15mm


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

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Invisiblesandman420
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Re: Scalpel not turning red [Re: Inthepit]
    #27295377 - 05/05/21 10:50 AM (3 years, 8 months ago)

I've only got 4 of my good pyrex brand left, the rest of mine are cheapos from amazon but they do the job. The expensive ones break easier, fuck!


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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