Home | Community | Message Board


Reliable Spores
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Buy Bali Kratom Powder, Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Amazon Agar

Jump to first unread post. Pages: 1
Offlineaberne
Stranger
 User Gallery
Registered: 04/25/21
Posts: 5
Last seen: 21 hours, 37 minutes
Interesting research paper I found - Cleaning up bacteria from agar plates
    #27281545 - 04/25/21 03:15 PM (10 days, 17 hours ago)

Apologies if this has already been posted on the forum.

After being frustrated by continuous bacterial contamination on my agar plates, I had a look at ways of cleaning this up, it always seemed to be bacteria and never mould in my case.

It seems some people opt for the antibiotic agar route but this isn't really the most sensible option. I came across this interesting paper in my search: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224635

It seems to be a Chinese research paper(?) that is peer-reviewed and open-access, so make of that what you will :grin:

I didn't fully understand the paper but what I gathered is you place contaminated spores/hyphae on a new agar plate. You place a sterile coverslip on top of it, and after a while, clean uncontaminated hyphae will emerge from the edges of the coverslip and continue growing. Please correct me if I have this horribly wrong. There seem to be a lot of interesting diagrams and results and such. If this is legit then this could be a fantastic addition to the community and make agar work way simpler :sun:

A coverslip is essentially a tiny piece of glass used in microscopy if you are unfamiliar with them

edit: To clarify, I properly understand it now. The point is that you cut a little ditch/hole in the agar and put the contaminated shit in there, the coverslip lies on top of the hole. The myc can apparently grow out of the hole but the nasty shit stays trapped in the hole.



Edited by aberne (05/04/21 05:54 AM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,082
Last seen: 6 hours, 44 minutes
Trusted Cultivator
Re: Interesting research paper I found - Cleaning up bacteria from agar plates [Re: aberne]
    #27282615 - 04/26/21 01:10 PM (9 days, 19 hours ago)

Nice. It's basically the same as our sammich tek. Kinda like the hot pour as well. I haven't really tried either much but thanks for linking the paper.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineaberne
Stranger
 User Gallery
Registered: 04/25/21
Posts: 5
Last seen: 21 hours, 37 minutes
Re: Interesting research paper I found - Cleaning up bacteria from agar plates *DELETED* [Re: verum subsequentis]
    #27283583 - 04/27/21 10:06 AM (8 days, 22 hours ago)

Post deleted by aberne

Reason for deletion: Placed this in my first post and marked it as an edit instead, cleans up the thread


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,082
Last seen: 6 hours, 44 minutes
Trusted Cultivator
Re: Interesting research paper I found - Cleaning up bacteria from agar plates [Re: aberne]
    #27283729 - 04/27/21 12:29 PM (8 days, 19 hours ago)

Yes.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinemistermycelium
Stranger
Registered: 08/19/20
Posts: 32
Last seen: 7 days, 13 hours
Re: Interesting research paper I found - Cleaning up bacteria from agar plates [Re: verum subsequentis]
    #27285422 - 04/28/21 07:03 PM (7 days, 13 hours ago)

Does look pretty similar. I still enjoy reading stuff like this so thanks!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinepinehurst
Stranger
 User Gallery
Registered: 08/09/19
Posts: 23
Last seen: 22 hours, 13 minutes
Re: Interesting research paper I found - Cleaning up bacteria from agar plates [Re: aberne]
    #27286503 - 04/29/21 01:40 PM (6 days, 18 hours ago)

Thanks OP!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineCyonic
Stranger
 User Gallery
Registered: 12/27/20
Posts: 86
Last seen: 1 day, 21 hours
Re: Interesting research paper I found - Cleaning up bacteria from agar plates [Re: pinehurst]
    #27290799 - 05/02/21 07:52 PM (3 days, 12 hours ago)

Cool!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineLovefields
Stranger
Registered: 03/06/21
Posts: 1
Last seen: 1 day, 7 hours
Re: Interesting research paper I found - Cleaning up bacteria from agar plates [Re: Cyonic]
    #27293157 - 05/04/21 12:17 AM (2 days, 8 hours ago)

Nice share. I've just ordered some coverslips to attempt cleaning up some contaminated MSS with this method.  Bought both glass and plastic versions.  Might find it tricky handling the glass coverslips without contaminating them or breaking them.

I didn't have much luck with my gentamicin agar plates, I might've messed up the ratio or I bought the wrong shit. I bought some gentamicin pills aimed for aquarium treatment.  I didn't try the hot pour method, I just transferred wedges. Still get green mold and some slime bacterial stuff growing. But I finally got some clean growth transferring to plates with H2O2 added.

Anyway, I'm excited to try this new technique.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineaberne
Stranger
 User Gallery
Registered: 04/25/21
Posts: 5
Last seen: 21 hours, 37 minutes
Re: Interesting research paper I found - Cleaning up bacteria from agar plates [Re: Lovefields]
    #27293321 - 05/04/21 05:52 AM (2 days, 2 hours ago)

Good luck. A lot of lab equipment from lab/educational suppliers comes pre-sterilised but if not, I'd just smother it in 70% iso to be honest.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Buy Bali Kratom Powder, Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Amazon Agar

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Best pre-made agar plates for Cultivation no-tone 3,184 9 01/30/03 12:31 PM
by no-tone
* Agar plate info? Joshua 2,859 6 11/02/01 06:36 PM
by dimitri211
* Effect of gravity on agar plate? Mycolomaniac 1,063 2 03/30/04 03:27 AM
by Mycolomaniac
* Alternatives to Agar Plates?
( 1 2 all )
GGreatOne234 4,392 20 09/11/02 09:14 AM
by GGreatOne234
* agar plate to liquid culture, how do u do it?? Visigoth 5,112 12 07/31/02 05:33 AM
by Suntzu
* Culture storage and agar plate alternative. SalviaEngland 1,544 2 01/18/03 07:48 PM
by ISH
* 1st and 2nd Flush from the Agar Plate! jelsert 750 1 12/07/02 03:28 PM
by Skikid16
* Re: Need Advice, storage of agar plates TriGuy 2,693 6 12/20/99 01:58 AM
by Ryche Hawk

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte, bodhisatta
322 topic views. 0 members, 3 guests and 5 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Mycotrop.com
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.019 seconds spending 0.005 seconds on 13 queries.