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Offlinebrikogen
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The Erythritol experiment. * 1
    #27293298 - 05/04/21 02:58 AM (3 years, 15 days ago)

I have come across a research paper that mentioned how erythritol affected fruiting body size and germination rate in plants, see:

Growth acceleration of plants and mushroom by erythritol

After doing a search I was surprised that from the vast knowledge on here no one gave this a go before.

Since I have 5kg of the stuff at home I thought I would give it a try on a cube run.

First of all the method is not scientific in any way as the sample size is 1 for each variation but here is what I have used:

  • Inoculated 4 bags of grain at around 1.3kg/bag wet grain from the same Golden Teacher liquid inoculate.
  • Prepared 3 tubs total
  • Tub #1 (Control): 1.5 grain bag, 250gr dry coco coir + 1000ml water
  • Tub #2 (0.15% Erythritol): 1.5 grain bag, 250gr dry coco coir + 1000ml water + 1.5gr Erythritol
  • Tub #3 (1% Erythritol):1 grain bag, 500gr dry coco coir + 2000ml water + 10gr Erythritol


You will note that Tub #3 has a higher coir to grain ratio (more than double) than the others which will also affect the result.

3 Pics below show the current state after 3 days.

https://imgur.com/a/xRZ4zE4

Edit: Pic 3 should say "Tub #3" not 2

Edited by brikogen (05/04/21 03:16 AM)

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Offlineaberne
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Re: The Erythritol experiment. [Re: brikogen]
    #27293324 - 05/04/21 04:00 AM (3 years, 15 days ago)

Interesting, thanks OP. Do report back when they fruit, perhaps the real differences will come then. Eagerly awaiting.
I also wonder how important it is to properly mix in the erythritol into the substrate or what the proper method is, as theres so little of it compared to everything else

Edited by aberne (05/04/21 04:08 AM)

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Offlinebrikogen
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Re: The Erythritol experiment. [Re: aberne]
    #27293332 - 05/04/21 04:31 AM (3 years, 15 days ago)

Quote:

aberne said:
Interesting, thanks OP. Do report back when they fruit, perhaps the real differences will come then. Eagerly awaiting.
I also wonder how important it is to properly mix in the erythritol into the substrate or what the proper method is, as theres so little of it compared to everything else




Will do, if anything happens at all. Also it's not mixed in dry, it's first dissolved in the water and then poored over the dry coir. From a mineral supplement point of view that can be quite high if expressed in PPM, ie 1500ppm (0.15%) and 10000ppm (1%).

Edited by brikogen (05/04/21 04:33 AM)

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Invisibleninja cat 09
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Re: The Erythritol experiment. [Re: brikogen]
    #27293906 - 05/04/21 12:07 PM (3 years, 15 days ago)

:popcorn:

Did you use an isolated strain? Or just MS -> LC?


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Offlinebrikogen
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Re: The Erythritol experiment. [Re: ninja cat 09] * 1
    #27293944 - 05/04/21 12:43 PM (3 years, 15 days ago)

Quote:

ninja cat 09 said:
:popcorn:

Did you use an isolated strain? Or just MS -> LC?




MS --> Agar --> Liquid Inoculate

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OfflineDERRAYLD
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Re: The Erythritol experiment. [Re: brikogen]
    #27296624 - 05/06/21 07:02 AM (3 years, 13 days ago)

Watching this closely,  considering giving it a try as well.

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InvisibleSmartattack
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Re: The Erythritol experiment. [Re: DERRAYLD]
    #27302149 - 05/09/21 09:19 PM (3 years, 10 days ago)

If myc loves it and flourishes, what will contams think of it?


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Re: The Erythritol experiment. [Re: Smartattack]
    #27303019 - 05/10/21 01:04 PM (3 years, 9 days ago)

Erythritol is supposed to have bacterial inhibiting effects, but it's only been observed at super high concentrations (I think something like 70g/lt). It'll also be interesting to see that!


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Offlinebrikogen
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Re: The Erythritol experiment. [Re: ninja cat 09] * 1
    #27303105 - 05/10/21 02:19 PM (3 years, 9 days ago)

Day 10.

Just put the tubs into fruiting. No contamination in any tub. Tub #3 does look slightly different than the other two, the mycelium looks more spiky (but this might also be due to the higher coir ratio) and it has especially good surface condition.

https://imgur.com/a/fvkbJCD

Edited by brikogen (05/10/21 02:25 PM)

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Offlinebrikogen
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Re: The Erythritol experiment. [Re: ninja cat 09]
    #27303150 - 05/10/21 03:10 PM (3 years, 9 days ago)

Quote:

ninja cat 09 said:
Erythritol is supposed to have bacterial inhibiting effects, but it's only been observed at super high concentrations (I think something like 70g/lt). It'll also be interesting to see that!




According to the paper it may be a double edged sword, too much might inhibit myc growth. They propose that the Erythritol is not used as a nutrient but somehow makes the myc more aggressive by using all the existing nutrients more efficiently?

Edited by brikogen (05/10/21 03:20 PM)

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OfflineDERRAYLD
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Re: The Erythritol experiment. [Re: brikogen]
    #27303781 - 05/11/21 03:34 AM (3 years, 8 days ago)

Interesting,  I was debating the way the mushroom uses the erythritol and assumed it was a nutrient base.

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Offlinebrikogen
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Re: The Erythritol experiment. [Re: DERRAYLD] * 1
    #27319651 - 05/23/21 07:14 AM (2 years, 11 months ago)

Currently on day 23 and the first flush is coming along well.

I was surprised by the fact that these were PE's and not GT's as the MS vial said. It's actually a happy little surprise as PE's are very rare around here.

https://imgur.com/a/YpTJDON

The "control" tub definitely has a less dense pinset than the other two. Tub #3 is currently winning on the most dense pinset.

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Offlinezeratul821
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Re: The Erythritol experiment. [Re: brikogen] * 1
    #27320932 - 05/24/21 08:13 AM (2 years, 11 months ago)

This pic from the paper is very encouraging


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OfflineDERRAYLD
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Re: The Erythritol experiment. [Re: zeratul821]
    #27321062 - 05/24/21 10:33 AM (2 years, 11 months ago)

Some promising results, can't ignore the multitude of variables involved but still promising.

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Offlinebrikogen
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Re: The Erythritol experiment. [Re: DERRAYLD]
    #27322020 - 05/25/21 02:09 AM (2 years, 11 months ago)

This will be the last progress pics of this flush before picking:

https://imgur.com/a/eVZIlwb

I however partially harvested Tub #2 as some started dropping spores.

Quote:

DERRAYLD said:
Some promising results, can't ignore the multitude of variables involved but still promising.




I agree the sample size needs to be higher, need to use a single clone, must use the same spawn to coir ratio... but this was merely a test, to mainly see whether erythritol didn't outright kill the mycelium at that concentration. Tub #3 with the highest erythritol does "seem" to have a denser initial pinset so that's promising. But again this might be due to multiple reasons, genetics, the spawn to coir ratio, water content, surface conditions...

In a future V2 experiment I would like to control these variables better and also so a dry weight comparison.

Quote:

zeratul821 said:
This pic from the paper is very encouraging






Indeed, and what's even more interesting is that their fruits were larger with erythritol but the tissue density did not decrease. So they weren't just holding more water weight.

Quote:

The fruiting bodies were larger in the
presence of erythritol, but the tissue density did not
decrease. These observations suggest that the supply of
erythritol leads to not an enlarged body, but growth
enhancement.

Although the
inner structure of fruiting bodies was observed under a
stereomicroscope, there was no decrease in the tissue
density of the fruiting body of the mushroom grown with
erythritol supply (data not shown)



Edited by brikogen (05/25/21 02:17 AM)

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OfflineDERRAYLD
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Re: The Erythritol experiment. [Re: brikogen]
    #27322053 - 05/25/21 03:41 AM (2 years, 11 months ago)

Interesting to note the color change across the 3 tubs as well, I wonder much the Erythritol played a part there.

Please do post some final weight figures for reference,  thanks for the experiment and sharing it.

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Offlinebrikogen
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Re: The Erythritol experiment. [Re: DERRAYLD]
    #27334578 - 06/04/21 12:18 AM (2 years, 11 months ago)

I'm picking as I go with the 2nd flush now but there is a noticeable fruiting size difference when comparing the control vs 0.15% erythritol


Edited by brikogen (06/04/21 12:30 AM)

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OfflineDERRAYLD
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Re: The Erythritol experiment. [Re: brikogen]
    #27334651 - 06/04/21 01:39 AM (2 years, 11 months ago)

Nice, any noticeable difference in stem thickness?
I assume that's not just water weight and they'll dry to a higher yield.

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Offlinebrikogen
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Re: The Erythritol experiment. [Re: DERRAYLD]
    #27334699 - 06/04/21 03:04 AM (2 years, 11 months ago)

Here's a cross section of one of the big one of the 0.15% run:

https://imgur.com/a/RbY0w0C

I know it doesn't say much but that looks like a healthy dense mushroom to me.

The heavy bruising is due to tissue sample cloning.

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Re: The Erythritol experiment. [Re: brikogen]
    #27341280 - 06/09/21 08:34 AM (2 years, 11 months ago)

Wait, you said you'd isolated the culture, so why are you cloning the stem?


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