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OfflineNajxwiinymuuxy
Teonanacani

Registered: 03/30/21
Posts: 41
Last seen: 2 years, 2 months
Are my spawn/jars of Panaeolus Goliath contaminated??? Plz, I'm in desperate need of a bit of your kind knowledge and wisdom
    #27291228 - 05/02/21 11:05 PM (3 years, 8 months ago)

Hello my friends. As you can see I'm kinda new in this wonderful forum, but have been reading and learning from people in shroomery for quite some time.

I know this may seem too noob or dumb, but I'm starting with this wonderful hobby/job, please could you enlighten me with some of yer knowledge and wisdom? πŸ™πŸ½πŸ™πŸ½πŸ™πŸ½ I'll be really grateful.

I will show you the photos, some of what I think is normal cottony-fluffy panaeolus mycelium and the others that look really weird, like shapeless flat mycelium stuck to the glass or grains, other that looks kinda grayish and I'm afraid might be cobweb, and there are jars that have little droplets of water in the glass, which I'm afraid might be wetspot, but I'm not sure.

Take a look please:

All of these next, I have the serious doubts if they're not healthy panaeolus mycelium and are contaminated or a sort of contamination:

















Is this cobweb?:
















Of this jar I have my doubts:
















And these jars I think are healthy:















I might add that the both the ones that I believe might be contaminated, and the cottony ones I believe are healthy, are from a mycelium slurry I made of a single healthy rhizomorphic tending mycelium growing on agar in Petri dish.
What do you guys think?


I will be very thankful forever if you can enlighten me a little or tell me your point of view, its a critical part of the process, as I need to mix soon these with bulk and don't want to be growing and caring for what would be an awful contaminated and contaminating batch, so it is crytical that I can identify properly if there is contaminants, and to distinguish good mycelium from cobweb or such sort of invaders.

Thanks and many thanks to you all πŸ™πŸ½πŸ™πŸ½πŸ™πŸ½

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OfflineRegis
Haben wir noch pepps?

Registered: 06/07/20
Posts: 274
Loc: Germany
Last seen: 22 days, 11 hours
Re: Are my spawn/jars of Panaeolus Goliath contaminated??? Plz, I'm in desperate need of a bit of your kind knowledge and wisdom [Re: Najxwiinymuuxy]
    #27291246 - 05/02/21 11:38 PM (3 years, 8 months ago)

I'm sorry mate, but you need to make photos with better quality.

The first jar looks sketchy. Uneven growth and some parts are creamy looking

Second and fourth jar I won't say anything, can't see shit.

Third jar looks sketchy, too. Wouldn't spawn it.

You could shake the fourth jar and see if it starts to recover in 24 hours

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Offlinetiptrippy
The Mechanic
Male


Registered: 09/09/20
Posts: 1,131
Loc: United States Flag
Last seen: 2 years, 5 months
Re: Are my spawn/jars of Panaeolus Goliath contaminated??? Plz, I'm in desperate need of a bit of your kind knowledge and wisdom [Re: Regis]
    #27291561 - 05/03/21 07:42 AM (3 years, 8 months ago)

I agree with what was said above. The pictures are terrible quality and very hard to see what's going on. That being said:

The ONLY jar that looks even close to healthy Pan myc is the one you suspect has cobweb. I would toss out the rest of them.

Panaeolus mycelium is never rhizomorphic on agar so if it was truly rhizo then you probably have Cubensis.

Here are some examples you should be looking for

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OfflineNajxwiinymuuxy
Teonanacani

Registered: 03/30/21
Posts: 41
Last seen: 2 years, 2 months
UPDATED BETTER QUALITY PICS [Re: Najxwiinymuuxy]
    #27292819 - 05/03/21 07:47 PM (3 years, 8 months ago)

UPDATED BETTER QUALITY PICS

Dear friends, sorry for the shitty pics I post earlier, I didn't have another camera for the  moment. I took some new pics with a better quality camera (still might be shitty, but is all I have for now), hope now they can be better appreciated. I took the at max resolution and posted them as huge thumbnails so details can be appreciated.

It's really confusing, as some parts of the colonized grain seems really fluffy-cottony, as a panaeolus would be, but other areas look flat or kinda rhizo, or just.. different... I may be confused, pardon me if I am, I've been  studying a lot and so, but the real complete knowledge comes with practice and learning through colleagues-teachers.

Someone said tha the one looking Cobwebby to me would be the healthy one.. I don't know it just looks so different to the others, but I think the textute it show may be because that was inoculated in a jar with a lot of horse poo in the substrate, as I was running out of millet and rye mix.

I hope you can shed some light. I'll be forever grateful.

Blessings.





























































And sorry if they're so many images, I saw no other way of showing you the different jars and their different angles and edges.

Edited by Najxwiinymuuxy (05/03/21 08:00 PM)

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OfflineNajxwiinymuuxy
Teonanacani

Registered: 03/30/21
Posts: 41
Last seen: 2 years, 2 months
Re: UPDATED BETTER QUALITY PICS [Re: Najxwiinymuuxy]
    #27292826 - 05/03/21 07:48 PM (3 years, 8 months ago)

Thank you very much my friend. I am sorry for that shitty quality, I took new pictures with a better camera, I hope you can have a better perspective now.

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OfflineNajxwiinymuuxy
Teonanacani

Registered: 03/30/21
Posts: 41
Last seen: 2 years, 2 months
Re: Are my spawn/jars of Panaeolus Goliath contaminated??? Plz, I'm in desperate need of a bit of your kind knowledge and wisdom [Re: Regis]
    #27292840 - 05/03/21 07:52 PM (3 years, 8 months ago)

Thanks a lot for taking the time, and your advice.
Indeed, the photos were very bad, I just took new ones, which I know are still not great, but one can appreciate better. What do you think?

I see this creamy growing in some parts, sometimes with the fluffy cottony mycelium, and I'm terribly confused now. Hope you can see something more clear now.

Many thanks, and blessings.

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Offlinetiptrippy
The Mechanic
Male


Registered: 09/09/20
Posts: 1,131
Loc: United States Flag
Last seen: 2 years, 5 months
Re: Are my spawn/jars of Panaeolus Goliath contaminated??? Plz, I'm in desperate need of a bit of your kind knowledge and wisdom [Re: Najxwiinymuuxy]
    #27292891 - 05/03/21 08:16 PM (3 years, 8 months ago)

Thank you for the better pictures. The only jars I would consider using are N,O,P,Q. I would toss the other jars into the compost. Stay away from anything thick and creamy as it is likely bacterial contamination. Cottony can be ok if there is no other weird looking mycelium in the jar with it. Usually it depends on what type of grain you are using. I'm not quite sure what you have in those jars but I'm sure it can make the mycelium look differently.

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OfflineNajxwiinymuuxy
Teonanacani

Registered: 03/30/21
Posts: 41
Last seen: 2 years, 2 months
Re: Are my spawn/jars of Panaeolus Goliath contaminated??? Plz, I'm in desperate need of a bit of your kind knowledge and wisdom [Re: tiptrippy]
    #27292955 - 05/03/21 08:43 PM (3 years, 8 months ago)

Thank you very much!! So the Z's,(z1 to 4), for example have some good looking fluffy mycelium, but also have that weird looking flat and creamy stuf, and some spot that even look rhyzomorphic grow... But the weird looking parts, according to what you've seen, may seem bacterial contaminatin, so no point in trying to use that one, right?

God, I took al the hygiene and sterile measure but still they got contaminated... may that be due that I made the agar myc slurry (even in a still air box), s maybe in that process contams entered? I also tied to disinfect the plastic petri dishes before pouring the agar, and also with a cotton with peroxide in the agar itself before injecting the liquid culture... I'm very inexpert in this and may be doing too much wrong things...
Anyway, I will toss all of my jars, apart from the one you said,that lookedlike comweb to me, but is is just healthy pan mycelium...
I have other set of jars I inoculated before with LC, and all look allright, with those fluffy cottony dense mycelium that even seem to form little balls, of those most look like that, but not any of those have that flat creamy patches, so they must be ok.

Any adice upon agar work and making agar slurry with the needle itself? and I mean also all the delicate work that  I do in the glovebox... I dunno, even I use that chamber I've got contams, so what may be wrong... As fot my jars they have dense polifyll filter at the center and two injection ports at the sides, with self healing high temp sylicone, so I thought they were really strong against contaminations entering, but I see nothing is guaranteed.

Thanks a lot my friend, your advice is unvaluable.

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OfflineNajxwiinymuuxy
Teonanacani

Registered: 03/30/21
Posts: 41
Last seen: 2 years, 2 months
Re: Are my spawn/jars of Panaeolus Goliath contaminated??? Plz, I'm in desperate need of a bit of your kind knowledge and wisdom [Re: tiptrippy]
    #27292989 - 05/03/21 08:52 PM (3 years, 8 months ago)

By the way, I used millet-rye, 75-25 % ratio as the substrate. And some well prepared manure in some of themas I was running out of grain.

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Offlinetiptrippy
The Mechanic
Male


Registered: 09/09/20
Posts: 1,131
Loc: United States Flag
Last seen: 2 years, 5 months
Re: Are my spawn/jars of Panaeolus Goliath contaminated??? Plz, I'm in desperate need of a bit of your kind knowledge and wisdom [Re: Najxwiinymuuxy]
    #27293025 - 05/03/21 09:10 PM (3 years, 8 months ago)

I wish I could be more help to you but unfortunately the language barrier is just too great. What is your native language? Maybe someone here can speak to you easier.

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OfflineNajxwiinymuuxy
Teonanacani

Registered: 03/30/21
Posts: 41
Last seen: 2 years, 2 months
Re: Are my spawn/jars of Panaeolus Goliath contaminated??? Plz, I'm in desperate need of a bit of your kind knowledge and wisdom [Re: tiptrippy]
    #27293110 - 05/03/21 09:43 PM (3 years, 8 months ago)

Thank you bro.
My native language is spanish, I'm from Mexico, and even if I can have errors in writing or speaking I understand 90% or more, I read specialized academic texts, so you can try to explain me what you want or need, I'll understand :smile:

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