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OfflineNightfox32197
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Still air box question
    #27291820 - 05/03/21 10:31 AM (3 years, 8 months ago)

Hey guys and girl. Im getting confused reading more about SABs. I keep seeing “no non sterile objects above sterile “ can someone explain this in better detail for me? If possible I really appreciate it

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OfflineLand TroutM
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Re: Still air box question [Re: Nightfox32197]
    #27291826 - 05/03/21 10:36 AM (3 years, 8 months ago)

As in don’t swing your hand over an open Petri dish, even a gloved hand wiped with bleach or iso could drop something and gravity could have it land on your dish.

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InvisibleSmartattack
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Re: Still air box question [Re: Land Trout]
    #27292039 - 05/03/21 01:21 PM (3 years, 8 months ago)

Yeah exactly. Like if you were to make a stack of items in the SAB, the top item had better be the known cleanest item and then the second most etc. That's not what you do in a SAB but for example. In other words gravity is the toilet paper and your asshole is the floor. Same concept in a flowhood, cleanest stuff goes closest to the airflow source.


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OfflineLand TroutM
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Re: Still air box question [Re: Smartattack]
    #27292091 - 05/03/21 01:44 PM (3 years, 8 months ago)

Was literally thinking of whipping ass away from vagina as an analogy.

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OfflineHindsight
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Re: Still air box question [Re: Land Trout]
    #27292791 - 05/03/21 07:38 PM (3 years, 8 months ago)

Just visualize every object raining bacteria and other contaminates from it, like dripping water, in a downward direction. So imagine your hand is soaking wet; everywhere you move it, it will be dripping water randomly. It applies to everything you put in there - your knife, your plates, your jars... everything. Wiping with ISO helps but doesn't eliminate bacteria and other contams.

In a SAB, especially since there are no air currents, things will just fall downward. So just don't move your hand, arm, tools, or any other objects over things that are supposed to be sterile and are opened up, like an agar plate or jar of grains. Instead, prop up the lid at an angle so it provides some cover, and insert your sterilized tool into the plate or jar, unload the transfer, remove the tool, then quickly close the lid. The only thing that was positioned directly over the sterile medium was the sterile tool and your sample.

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