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OfflinePurposefullyVague
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Registered: 03/10/21
Posts: 4
Loc: United States
Last seen: 3 years, 2 months
ID this contam AND inoc question
    #27291508 - 05/03/21 06:32 AM (3 years, 8 months ago)

Hello all and thanks in advance for the help. Quick background: I'm a learner/lurker for a year. The damn Facebook group suggested you to me actually is how all of this began. Im an Adamant tek-follower. I Assembled materials and began at the end of March. I harvested my First fruits this weekend!!!

I chose PF tek (inoculation/colonization method); shredded to bulk coir (fruiting method) in 6qt shoeboxes per BODS easy AF list. Have had tremendous success and only had trich one time on my beautiful myc in one jar that i tried to open-air inoculate and also suspect the dry verm layer failed (also i use SAB even with pf tek for sure. Now when i start brf tek and eventually agar im comfortable and familiar). Then i had penicillium in EVERY jar in one batch ONLY at EVERY inoculation point. Bad inoculant, right? Well I am no uneducated simpleton who is going to blame THE most reputable vendor BC It wasnt the vendor. And im not dirty enough to try to get a freebie (SW) over my mistake. it was because I uncapped, used on 4 jars, then recapped and reused far too casually on 7 more and it cost me 7cc of my fat ass freebie pf classic syringe. ANYWAYS..

Question 1:.the question i came to ask is "can you ID this contam?".

This jar was prepared at the same time as ALL of my other jars of a particular batch- however since it is the wrong size jar AND because  "i wanted to see what contamination caused by a lack of sterilization looks like so i didn't sterilize this jar at all. Just placed the mix in, added a dry verm layer like normal, then sat it in a closet

I assume this is the endospores having taken off? There are No holes in the lid but I loosened the ring slightly last week for Ge for it.

Anyways is there anything i can really learn from this? Ive had 97% success rate and No contams in my shoeboxes yet (which i understand comes from the spawn so i never tried spore to grain or any other frowned upon bad advice nonsense.)

Question 2: HOWEVER i did receive a beast of a melmak og spore swab that i won in a contest :smile: . I dont have a PC for agar or grain prep so my plan is to put it to BRF paste that i have managed to successfully create with simple steam sterilization due to the widely known low endospore and such permissible qualities

After ive cleaned up the melmak on the brf paste thru transfers just like agar (and even better at isolating bacteria on a plate than agar..check Pokex thread if u dont believe lil ol me) ..how do i go about inoculating a brf cake with mycelium prepared on a brf plate? I usually use MSS and inject into small holes past a dry verm layer. Do I prepare some "broke boi tek" brown rice (which i have shied away from as its not a well respected tek..but it may be the solution for me here as i can't very well prepare grains steam sterilizing them OR use a cake cuz of the dry verm layer, unless someone has a method?

Awaitint answer anxiously


--------------------
Soaking in all of the knowledge that i can from the legends who came before me.

Current status: *PF Tek* (inoculation/colonization method)
*Shred to bulk coir in 6qt shoeboxes* (fruiting method)
*BRF paste*: for cloning fruits, utilizing swabs and cleaning shit up.

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