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Atomsplit



Registered: 01/16/21
Posts: 1,505
Loc: SAB
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27309221 - 05/15/21 01:32 AM (2 years, 8 months ago) |
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Randomly found this portable ac on amzn for under $50
Nuaer Portable Air Conditioner, Air Cooler with 600ML Water Tank, Desktop Air Conditioning Fan with 7 Colors Lights & LED Display, 2/4Timer, 3 Speeds, Suitable for Bedroom, Office and Home.
Don't know if that works for you. I really wanna see you grow some canopies.
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Ashtray's Questions and Answer Thread [Re: Atomsplit] 1
#27309223 - 05/15/21 01:34 AM (2 years, 8 months ago) |
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Quote:
Atomsplit said: Randomly found this portable ac on amzn for under $50
Nuaer Portable Air Conditioner, Air Cooler with 600ML Water Tank, Desktop Air Conditioning Fan with 7 Colors Lights & LED Display, 2/4Timer, 3 Speeds, Suitable for Bedroom, Office and Home.
Don't know if that works for you. I really wanna see you grow some canopies.

Thanks mate. Im getting close, I know I am. I have a lot of hope for this next round im about to knock up with agar.
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310017 - 05/15/21 04:05 PM (2 years, 8 months ago) |
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Quick question: How long from MSS do agar plates tend to take to pin? Im trying to confirm that what im looking at is healthy mush myc and my oldest plates are from 4/29. None have pinned yet but I think thats prolly normal? How long should I expect them to take to fully colonize the plates and pin if theyre healthy?
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310143 - 05/15/21 05:39 PM (2 years, 8 months ago) |
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Another quick (but kind of time sensitive) question. Im PCing some agar mix rn to pour into petris but I was wondering do I have to do anything to sanatize the petris? I havent taken them out of their sleeve and I figure that I should do so in my SAB when Im almost ready to pour but wont they be likely to have contams or does the hot agar kill most of that? or just the fact of pouring in the SAB? Do I need to spray the outside or inside of the plates w iso and let it evap first?
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Atomsplit



Registered: 01/16/21
Posts: 1,505
Loc: SAB
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310165 - 05/15/21 05:58 PM (2 years, 8 months ago) |
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Quote:
Ashtray161 said: Another quick (but kind of time sensitive) question. Im PCing some agar mix rn to pour into petris but I was wondering do I have to do anything to sanatize the petris?
No. just unsleeve them in your sab. They are already sanitized sterilized
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Edited by Atomsplit (05/15/21 10:46 PM)
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
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Re: Ashtray's Questions and Answer Thread [Re: Atomsplit] 1
#27310170 - 05/15/21 06:04 PM (2 years, 8 months ago) |
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Quote:
Atomsplit said:
Quote:
Ashtray161 said: Another quick (but kind of time sensitive) question. Im PCing some agar mix rn to pour into petris but I was wondering do I have to do anything to sanatize the petris?
No unsleeve in sab. They are already sanitized
awesome thank u. This is my first time ever using a PC. Its a big ol pot lol
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310181 - 05/15/21 06:11 PM (2 years, 8 months ago) |
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Im feeling like a proper armature mycologist today lol. Making some plates right now so I can do some 4th transfers from the prebought dishes and then making some grain jars to toss that shit in 
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Atomsplit



Registered: 01/16/21
Posts: 1,505
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310208 - 05/15/21 06:30 PM (2 years, 8 months ago) |
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Have fun! Looking forward to seeing you canopies my friend!
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Ashtray's Questions and Answer Thread [Re: Atomsplit] 1
#27310211 - 05/15/21 06:30 PM (2 years, 8 months ago) |
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is it okay that my PC is kind of shaking? o.0
I put a good decent amount of water in it, up to the neck of where the agar was in the bottle cuz thats what BODs tek said to do lol
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
Edited by Ashtray161 (05/15/21 06:32 PM)
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psh
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Registered: 05/06/21
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310230 - 05/15/21 06:51 PM (2 years, 8 months ago) |
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Do you have a 23 quart presto? You're only supposed to use 12 cups of water I believe.
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Ashtray161
SettledNomad



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Re: Ashtray's Questions and Answer Thread [Re: psh] 1
#27310377 - 05/15/21 08:44 PM (2 years, 8 months ago) |
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alright folks, ive done it. (i hope) Ive made 500ml of sterilized agar solution. I just pulled it from the PC about 15 mins ago and its still pretty hot to touch. I just got to a step I didnt think fully through thou and I dont have any way to measure the temp. How long roughly should I wait or how hot should it be to touch when I start pouring?
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Ashtray161
SettledNomad



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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310403 - 05/15/21 09:06 PM (2 years, 8 months ago) |
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Sorry i dont mean to bump my own thread but I dont want it to get too cold... Its still *just* too hot to handle comfortably. Im assuming that means its around 135-150? At 120 or so it should be easy to hold without it burning?
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310406 - 05/15/21 09:07 PM (2 years, 8 months ago) |
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You don't need to measure the temp with anything other than your hands. If you can pour it without melting your fingers it's gtg.
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Ashtray161
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Re: Ashtray's Questions and Answer Thread [Re: Stipe-n Cap] 1
#27310410 - 05/15/21 09:09 PM (2 years, 8 months ago) |
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Quote:
p9hu7 said: You don't need to measure the temp with anything other than your hands. If you can pour it without melting your fingers it's gtg.
Right on, BODs tek talks about condensation being a problem if u pour too hot so I just wanted to be sure i wasnt gonna ruin em or if theres a rule of thumb (which u just shared lmao) to know the sweet spot
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Stipe-n Cap


Registered: 08/04/12
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310446 - 05/15/21 09:45 PM (2 years, 8 months ago) |
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Keep in mind that if you get lots of condensation you can just leave your plates unwrapped and stacked in the SAB overnight. This should help with condensation if you get too much.
The thermal mass of the stack will help evaporate the condensation, except for maybe the top plate of the stack.
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Ashtray161
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Re: Ashtray's Questions and Answer Thread [Re: Stipe-n Cap] 1
#27310479 - 05/15/21 10:09 PM (2 years, 8 months ago) |
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Quote:
p9hu7 said: Keep in mind that if you get lots of condensation you can just leave your plates unwrapped and stacked in the SAB overnight. This should help with condensation if you get too much.
The thermal mass of the stack will help evaporate the condensation, except for maybe the top plate of the stack.
Yeah i learned 2 important lessons. Dont overfill, and be patient. They all fogged up xD I have em stacked and unwrapped in the SAB hopefully theyll be ok tomorrow lol
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Atomsplit



Registered: 01/16/21
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310499 - 05/15/21 10:25 PM (2 years, 8 months ago) |
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I've poured petri where there was quite a bit of condensation. It was enough to roll down from the lid onto the surface of the agar.
About a month later & even wrapped- condensation and water on agar are both gone
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Ashtray161
SettledNomad



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Re: Ashtray's Questions and Answer Thread [Re: Atomsplit] 1
#27310504 - 05/15/21 10:28 PM (2 years, 8 months ago) |
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Quote:
Atomsplit said: I've poured petri where there was quite a bit of condensation. It was enough to roll down from the lid onto the surface of the agar.
About a month later & even wrapped- condensation and water on agar are both gone
Is the condensation a possible contam vector? Or just a pain for being able to see the culture?
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(You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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Atomsplit



Registered: 01/16/21
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310508 - 05/15/21 10:39 PM (2 years, 8 months ago) |
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Its your first pour in a sab. They most likely wont come out as perfect as you would hope.
I think you will be ok. Just remember to wrap the plates carefully INSIDE your sab when and if you decide to wrap them.
Im a newb. I hope my contamination rates will get lower with the more practice and time I put in.
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Edited by Atomsplit (05/15/21 10:50 PM)
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NickShade
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Re: Ashtray's Questions and Answer Thread [Re: Ashtray161] 1
#27310533 - 05/15/21 11:09 PM (2 years, 8 months ago) |
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Quote:
Ashtray161 said: is it okay that my PC is kind of shaking? o.0
I put a good decent amount of water in it, up to the neck of where the agar was in the bottle cuz thats what BODs tek said to do lol
If youre using 500ml media bottles and filling the cooker up to the neck, that sounds like a lot of water mate. 3L will suffice, which is both the manufacturer and Bod's recommendation
-------------------- bodhisatta said: figure it out listen to the cake
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