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Inthepit
Aum Mani Padme Hum


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 1 day
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PESA from last year's Clone, success! 2
#27290017 - 05/02/21 07:18 AM (3 years, 8 months ago) |
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Hoorah! PESA in 2 months! Three shoeboxes! Happy camper!
     
Clone from LAGM20 a year ago, was kept in the fridge in petri and baggy.
So...took the clone to T5 made 5 LI, liquid inoculate, 10mm sterilized water, wedges, shook like crazy poured 5 quarts of Oats from 5 LIs 4 Qrts to 4 Shoeboxes, one quart never went off 3 Shoeboxes and one SB contamed 3 SBs fit in a tote for fruiting after colonization in the SBs, I had to duct tape the holes to get condensation on the walls yes we twisted and pulled them out, it left a very clean surface cut the bottoms off and into the dehydrator for 24 hrs! then most go into "0" size capsules and the awesome ALL-IN capsule loader ============================================================================
 I side watered and let sit 24hrs, then one needed pouring excess off. 5/2
So...what are my options with these tiny second flush PESA pins in the red outline? Three shoeboxes, all tiny pins, with just this one decent cluster. 7 days later.
Are they aborts, just pick'em out and side water? Let'em go, they're just on their own schedule? Their cute widdle heads are not discolored... 
============================================================================= Mateja said: "I'd definitely dunk for more than 12h, here's an experiment I did long time ago regarding water absorption in BRF cakes..." https://www.shroomery.org/forums/showflat.php/Number/24739690 So what about the comment in that thread that less hydration led to faster colonization"\. When you looked into his experiments, were you not convinced.
I see a lot of variation in dunking, I started with the shoebox tek where she stacked shoeboxes to make sure the substrate was under water for, um, 3 hours I think...
Now I'm in the camp off "side water" for a couple hours and pour off excess.
I'm just thinking MORE is not necessarily better...
Edit: Oh shoot, old thread, sorry, was lost in researching pins! Maybe I should have PM'd him.
Edited by Inthepit (05/14/21 05:40 AM)
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Sockadin



Registered: 01/03/10
Posts: 7,266
Last seen: 1 month, 22 days
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Re: PESA from last year's Clone, success! [Re: Inthepit]
#27290093 - 05/02/21 08:19 AM (3 years, 8 months ago) |
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Those are really healthy looking fruits. Never ran PESA before but I like the long stems.
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Inthepit
Aum Mani Padme Hum


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 1 day
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Re: PESA from last year's Clone, success! [Re: Sockadin]
#27301145 - 05/09/21 05:47 AM (3 years, 8 months ago) |
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Hello Fellow Shroomites!
Thank you Mushboy, keepin it safe!
 I side watered and let sit 24hrs, then one needed pouring excess off. 5/2
So...what are my options with these tiny second flush PESA pins in the red outline? Three shoeboxes, all tiny pins, with just this one decent cluster. 7 days later.
Are they aborts, just pick'em out and side water? Let'em go, they're just on their own schedule? Their cute widdle heads are not discolored...
Edited by Inthepit (05/09/21 06:24 AM)
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