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OfflineInthepit
Aum Mani Padme Hum
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Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 1 day
PESA from last year's Clone, success! * 2
    #27290017 - 05/02/21 07:18 AM (3 years, 8 months ago)

Hoorah!    PESA in 2 months!    Three shoeboxes!    Happy camper!

            :cubie::cubie:
              :tongue:     

Clone from LAGM20 a year ago, was kept in the fridge in petri and baggy.

So...
    took the clone to T5
    made 5 LI, liquid inoculate, 10mm sterilized water, wedges, shook like crazy
    poured 5 quarts of Oats from 5 LIs
    4 Qrts to 4 Shoeboxes, one quart never went off
    3 Shoeboxes and one SB contamed
    3 SBs fit in a tote for fruiting after colonization in the SBs, I had to duct tape the holes to get condensation on the walls
    yes we twisted and pulled them out, it left a very clean surface
    cut the bottoms off and into the dehydrator for 24 hrs!
    then most go into "0" size capsules and the awesome ALL-IN capsule loader

============================================================================


                     
I side watered and let sit 24hrs, then one needed pouring excess off. 5/2

So...what are my options with these tiny second flush PESA pins in the red outline?
Three shoeboxes, all tiny pins, with just this one decent cluster. 7 days later.
    Are they aborts, just pick'em out and side water?
    Let'em go, they're just on their own schedule?

Their cute widdle heads are not discolored...  :rofl:

=============================================================================
Mateja said:
"I'd definitely dunk for more than 12h, here's an experiment I did long time ago regarding water absorption in BRF cakes..."
https://www.shroomery.org/forums/showflat.php/Number/24739690

So what about the comment in that thread that less hydration led to faster colonization"\.
When you looked into his experiments, were you not convinced.

I see a lot of variation in dunking, I started with the shoebox tek where
she stacked shoeboxes to make sure the substrate was under water for, um, 3 hours I think...

Now I'm in the camp off "side water" for a couple hours and pour off excess.

I'm just thinking MORE is not necessarily better...

Edit: Oh shoot, old thread, sorry, was lost in researching pins! Maybe I should have PM'd him.

Edited by Inthepit (05/14/21 05:40 AM)

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OfflineSockadin
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Registered: 01/03/10
Posts: 7,266
Last seen: 1 month, 22 days
Trusted Cultivator
Re: PESA from last year's Clone, success! [Re: Inthepit]
    #27290093 - 05/02/21 08:19 AM (3 years, 8 months ago)

:howyoudoing:

Those are really healthy looking fruits. Never ran PESA before but I like the long stems.

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OfflineInthepit
Aum Mani Padme Hum
Male User Gallery

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 1 day
Re: PESA from last year's Clone, success! [Re: Sockadin]
    #27301145 - 05/09/21 05:47 AM (3 years, 8 months ago)

Hello Fellow Shroomites!

Thank you Mushboy, keepin it safe!

                     
I side watered and let sit 24hrs, then one needed pouring excess off. 5/2

So...what are my options with these tiny second flush PESA pins in the red outline?
Three shoeboxes, all tiny pins, with just this one decent cluster. 7 days later.
    Are they aborts, just pick'em out and side water?
    Let'em go, they're just on their own schedule?

Their cute widdle heads are not discolored...  :rofl:


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

Edited by Inthepit (05/09/21 06:24 AM)

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