Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
OfflineCantStandYa
Stranger

Registered: 04/30/21
Posts: 38
Last seen: 3 years, 8 months
Really Strange Info on Agar
    #27287565 - 04/30/21 07:35 AM (3 years, 8 months ago)

Im not going to name where I got this from but its a popular spore vendor. This information sounds like totally opposite of so much ive read on here. Anyone want to weigh in or where this comes from?

"Spore syringes really aren’t good for using agar. Some times they will show bacteria on agar, yet when you put them directly to grain there is no signs of bacteria. On agar, the mushroom mycelium can not compete against the trace amounts of bacteria. But once put directly to grain, the mycelium easily outcompetes the trace amounts of bacteria and grows on the healthy mycelium. We learned this from Paul Stamets, one of the greatest mycologist of our generation. Because of this we don’t recommend using spore syringes on agar, unless you happen to be using gentamicin sulfate antibiotic powder. We use gentamicin in our agar for testing spore syringes. So our test batches will not show bacteria. Yours might show bacteria on agar if you’re not using gentamicin. Gentamicin is hard to find but you can find it at here at *********.com. If you want to use agar for strain isolation etc.. or making liquid cultures, then we recommend using spore prints. You should also be aware that using too much spore solution on agar will cause bacteria. Only a few drops is needed. If you still want to use spore syringes on agar, then use gentamicin for best results.

About 1 in 100 spore prints will naturally have trace amounts of bacteria. Once we combine many spore prints in our batch spore solution, this could cause trace amounts of bacteria in some batches of spore solution."

Edited by CantStandYa (04/30/21 08:03 AM)

Extras: Filter Print Post Top
OfflineSingularFusion
 User Gallery

Registered: 10/31/18
Posts: 1,303
Last seen: 1 month, 2 days
Re: Really Strange Info on Agar [Re: CantStandYa]
    #27287580 - 04/30/21 07:53 AM (3 years, 8 months ago)

No such thing as 100% clean syringes in my understanding, unless perhaps the fruit that was printed to make the resulting syringes had been culted in sterile conditions...

But that's not how cult is done in the actual real world

That's why you put syringes to agar to clean up with transfers

Or alternatively do the PF tek with syringes and clone a fruit to agar

Syringes straight to grain is pure gambling, sometimes you win, sometimes you lose

All the best with it

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* using agar? downforpot 2,318 6 12/02/01 07:00 PM
by Anno
* test tubes agar fungophiliac 3,280 8 06/19/20 09:41 PM
by gorilliam
* Miscellaneous info on Azures and Cyans 003 5,409 7 06/07/05 07:59 AM
by Anno
* Anyone use Gentamicin Eye Drops? District 1,997 17 03/14/16 04:54 PM
by eatyualive
* Contam Sandwich Agar Technique... ATWAR 6,085 8 06/29/04 07:52 PM
by ATWAR
* Experiment/Info: BRF vs. Whole Wheat vs. Soy CapACreep 1,073 1 11/13/02 05:18 PM
by Shrooms4e
* Black "smoke traces" Junk 793 2 02/04/05 10:20 AM
by SunshinePounder
* Gentamicin Sulfate Solution in Liquid Culture Dr.Mykull 2,015 2 09/18/08 12:02 PM
by Dr.Mykull

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
193 topic views. 32 members, 88 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.019 seconds spending 0.008 seconds on 12 queries.