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Offlinesharkjazz
Stranger
Registered: 12/16/20
Posts: 263
Last seen: 3 years, 8 months
trich (green growth) after first flush of last 5 shoeboxes
    #27286760 - 04/29/21 03:31 PM (3 years, 8 months ago)

Am still quite new to this, but think I've progressed enough away from getting started forum to come play with the big boys in mush. cult.

My first shoebox (12L) went for 5 flushes (admittedly very weak by the end but was still fruiting so I let it do it's thing). Now however, I get tric (Well a green growth) after the first flush every time.

Sometimes it's a day after picking the flush. I've noticed a small patch whilst picking the flush or it's just as a mighty pinset is starting.


So from my reading, it says "it's the spawn". Ok, how can I prove this? Fix this?

I've never had tric in a jar, although people have said my jars look bacterial (some have been worse than others, but after some agar experiments (grain2agar then clean agar to jars) *I* don't see the tell tale signs of bacterial infections in the jars. But the tric kept coming so I went grain to agar to BRF (good for bacteria prevention I read) to agar to grain, yet still after the first flush the tric is there.

I've currently got 9 small jars (200ml) 100% colonised from an agar slice (attempting to isolate but first time so ...) jars look white to me, I was going to go per (isolate) jar g2g to 2x500ml jar then use those in a shoebox. But perhaps I should do some more agar work to clean up.

Is the issue definitely the spawn ? Or could it be the mold in the air?

Bit of background. Am in the tropics so is hot and humid. I have the basic gear, SAB, PC, agar, plates etc.


Any ideas? Advice?

Cheers

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OfflineFuzzFungi
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Registered: 03/19/21
Posts: 20
Last seen: 3 years, 8 months
Re: trich (green growth) after first flush of last 5 shoeboxes [Re: sharkjazz]
    #27286880 - 04/29/21 05:08 PM (3 years, 8 months ago)

I work with agar and in front of a flow-hood so I am 99% confident that my grain is clean.

In my case, the times I got Tric was from improper pasteurization using the bucket Tek for my Mono Tubs. I know let my bulk substrate pasteurize and cool over night.

If you are sure you are doing the basics properly, my only advice is:

1. Shake test.
-Do your jars stall after you shake or does the mycelium quickly recover?
-If they stall, toss them and review your SAB and PC technique.
2. Try invitro fruiting to rule out your casing layer or bulk substrate
-If it is still getting mold then your grain or the environment is the issue.

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Offlinetiptrippy
The Mechanic
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Registered: 09/09/20
Posts: 1,131
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Last seen: 2 years, 5 months
Re: trich (green growth) after first flush of last 5 shoeboxes [Re: FuzzFungi]
    #27286964 - 04/29/21 06:12 PM (3 years, 8 months ago)

If you went through all that rigorous testing of your spawn to make sure it was clean, and you successfully get a first flush then I'm leaning towards your substrate being the issue.

What are you using and how do you prepare it?

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Offlinesharkjazz
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Registered: 12/16/20
Posts: 263
Last seen: 3 years, 8 months
Re: trich (green growth) after first flush of last 5 shoeboxes [Re: tiptrippy]
    #27287821 - 04/30/21 12:29 PM (3 years, 8 months ago)

Quote:

FuzzFungi said:

If you are sure you are doing the basics properly, my only advice is:

1. Shake test.
-Do your jars stall after you shake or does the mycelium quickly recover?
-If they stall, toss them and review your SAB and PC technique.




After a shake, the myc seems gone for 24 hours, 48hours you can see it coming back 72 hours is back to how it was or better (depends when shake 20% colonised vs 80% colonised). Not sure if this is good or not


Quote:


2. Try invitro fruiting to rule out your casing layer or bulk substrate
-If it is still getting mold then your grain or the environment is the issue.







You mean just let it pin in the jar? I did this previously (was busy, not intentionally) but had mushrooms growing in the jar with no contams. I then used the jar, cut the mushrooms off in a casing. Contammed after first flush.




Quote:

tiptrippy said:
If you went through all that rigorous testing of your spawn to make sure it was clean, and you successfully get a first flush then I'm leaning towards your substrate being the issue.

What are you using and how do you prepare it?




Quote:

FuzzFungi said:

In my case, the times I got Tric was from improper pasteurization using the bucket Tek for my Mono Tubs. I know let my bulk substrate pasteurize and cool over night.





Ok, I think you two might be on to something. The first shoebox I did, I *attempted* to pasteruised (substrate in oven bags in a pot on the cooker. But my thermoneter broke, the oven bags leaked and I thought was a disaster. I had squeezed the substrate and it went on to 5 flushes.

Since then I've used bucket tek. I have loose coir, but usually go for
1.5L coir
1.5L verm

then add 1L boiling water to bucket, leave over night.The boiling water does NOT cover the substrate, so is steamed through most of it.


All things considered, this is most likelly the issue.

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