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InvisibleWrightii
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The Official Hericium Thread * 6
    #27286241 - 04/29/21 07:47 AM (2 years, 8 months ago)


I am in no way an expert/authority on this topic (so feel free to correct or add) but I’ve experienced some grows and will try to convey what I found/experienced 😊. I'm sure there's a 100 other ways, this is just what works for me
.

This thread tries to introduce you to growing Hericium. Known for its delicate taste and possible nootropic effects it's a mushroom worth growing!
It is most useful to those new to growing Hericium, but with some background on mushroom cultivation in general. I split it up in roughly 3 parts.
The first part is about agar and cultures, the following about substrates, the last talks about grow methods and fruiting. If you feel something is missing let me know.


Content

1  - Agar
2  - LC
3  - Cloning fruits
4  - Storing cultures

5  - Grain spawn
6  - Bulk substrates

7  - Growing in bags
8  - Growing in jars
9  - Dowels and growing on logs
10 - Growing other ways
11 - Fruiting conditions


My grows are with Hericium Corraloides btw, a close relative of lions mane.
Quite sure the techniques and subs and whatever work for both mushrooms though!


1. Agar
  • Keep it simple: LME/PDA
  • Mycelium not consistent in appearance

Get ¨perfect results with simple LME agar (10 gr LME, 10 gr agar, 500 ml water). Additives are not really needed in my experience, they just increase contam risks. PDA also functioned just fine. The mycelium is not that picky, compared to most shrooms. Colonization with temps on the lower side (18 Celsius) went just fine. I even prefer a bit lower temps, as contams are less likely to pop up, The mycelium can take on quite some different appearances in my experience. Some parts can look whispy, some rhizomorphic, while other parts maybe even start to fruit. This can all happen on the same plate (PDA):

When using agar for transfers always try to take a piece of the side of the culture, that is still actively growing.

2. LC
  • 2% LME
  • Keep it moving

I’ve had good results with a simple 2% LME in water. Growth is quick and the mycelium very sturdy. A magnet stirrer is a huge plus, as breaking the mycelium is not that easy. Older cultures eventually form almost solid dots of mycelium. I inoculate all my LC’s by simply dropping in a small agar wedge.
I’ve used the LC’s to successfully inoculate grains, jars and bags with woodchips and dowels.

LC after 8 days with daily stirring:


3. Cloning fruits
  • Contamination prone due to fruit morphology
  • Use rapid transfers to obtain a clean culture

I obtained my running culture by cloning a wild Hericium. Cloning this mushroom can be somewhat challenging as the fruit has this weird shape that can house all the contams. Especially when cloning wild mushrooms. I found with cloning that the more the better. Try 20 small fruit pieces for cloning, and start looking for mycelium. Work as scheduled as possible during this stage. Older plates will have higher chances of (sporulating) contams, so start transferring as soon as a tiny bit of growth is visible. Again larger numbers increase the rate of success. 2 or 3 transfers later your often running a clean culture.
If you do not manage this way, using antibiotic enriched plates can make everything much and much easier. These however are not available everywhere, and using antibiotics IMO is a bit of a no go for hobby purposes.
Another technique I’ve heard of but never tried, is making an agar sandwich. Pour some plates with agar like usual, but with about half of the volume you would normally do. Transfer your fruit on to this plate, and grow it for a day or 2. Now comes the sandwich part; brew up a new batch of agar and let it cool down as much as possible while still being liquid. This agar is then poured over the first layer of agar with fruit on it in the original dish. This would supposedly trap most contamination while the fungus grows through it to the top and spreads. Can’t vouch for its effectiveness, but sounds pretty smart.

4. Storage of cultures
This species tolerates cold well and is therefore quite storage friendly.
Making slants with a normal agar LME recipe with an added piece of wood for longevity is no issue. So far, slants remain viable after storage at 4 Celsius for at least a year. It might very well be longer, but have not made it that far yet.
Slant of Hericium:

Slant of Hericium that started fruiting in the fridge:

Jars with inoculated grains or wood can easily be stored in the fridge and remain good and viable for months on end. This fungus really seems to be doing well with (recovering from) cold dormancy. To make a spore print an adult specimen sporulating (you will notice a whiteish hue on things the spores land on) is placed on some tin foil, covered and left for a couple of hours. Remove shroom, fold tinfoil and voila.


5. Grain spawn
  • Not picky and strong
  • WBS or rye are success

I’ve had success with both rye and WBS. Both were prepared like standard grainspawn. Inoculation is possible with agar wedges, G2G and LC’s. Colonization is quite quick and a bit colder temps (18 Celsius) seem no problem. This might however differ for other non Corraloides Hericium. Mycelium can at first barely be visible while already having grown through the complete substrate. With maturation the mycelium thickens and becomes much more visible. Small fruits can form in the jars, even when they don’t seem fully colonized. Start using the jars when all the spawn is visibly colonized. When small fruits are present I just break those up together with the mycelium. They should be sterile and form no issues. Fishing them out might increase the chances of contams.

2 fully consolidated jars of rye (ignore LC):

Got this jar out of the fridge today. Some yellow metabolite spots, but 100% sure that if I’d bulk it it would do just fine 😊 :


6. Bulk substrates
  • Woodchips all the way
  • Mixed particle sizes found to work well

Hericium is a definite wood lover. I’ve had success with chips of both Pseudo acacia/robinia and beech. When preparing the wood I always try to make a substrate that has different particle sizes. Beech chips of different sizes (petshop) are simmered for a couple for hours to achieve full hydration (check by breaking chips) and strained to field capacity after. This mix of chips is then supplemented with beech sawdust, in a way that the vermiculite is covered with BRF in PF-tek. Keep adding water to maintain field capacity.  An example recipe would be:
2 cups large chips –
2 cups medium chips –
2 cups small chips –
2 cups sawdust.
½ cup of gypsum could also be added.

This mixture contains only wood as substrate, and is therefore not very sensitive to contams. It can be pasteurized instead of sterilized if one prefers so.
A more nutritious substrate that might result in somewhat bigger yields would be achieved by adding wheat bran or soy husks.The latter I’ve never tried. A recipe I found to work fine:
3 cups large chips –
3 cups medium chips –
3 cups fine chips –
1 cup sawdust –
2 cups wheat bran or soy husks.

Again gypsum is optional. I always aim for 20% of wheat bran. Add the wheat bran together with the sawdust while again maintaining field capacity. Since this sub is more nutritious and prone to contams it does need to be sterilized for 90 min at 15 psi.
Bulk substrates for hericium are best kept/made with a pH ranging from 5.0 to 6.5
Hydrated chips and sawdust+bran before mixing:

After mixing and spraying with water to get field capacity:


Although it should really be avoided, I found this mushroom is still able to fruit while the substrate is contaminated. However: this is only the case when the contam settles after full colonization of the substrate (i.e. 2nd flush)


7. Growing in bags
  • Very easy, clean and simple
  • Use above mentioned substrate
  • Lot of plastic waste :frown:


Growing Hericium in bags works well. The bags should be filled with before mentioned bulk subs, and can be inoculated with grain spawn or straight with LC. I found bags with the wheat bran recipe that were sterilized to perform best overall.  Colonization of bags can (dependent on the amount of spawn used) be extremely quick.
Whenever is see some solid spots of mycelium I thoroughly mix it within the bag. I found that doing this results in an more even colonization. Not doing this can often results in fruiting within the bag although the mycelium is far from consolidation throughout the whole bag. This mixing is normally 4 or 5 days after inoculation. I found that fully consolidated bags result in the nicest flushes with dense fruits. Fruiting while the mycelium is still colonizing is definitely possible though. It will just keep on growing during the following flushes. 

Right after inoculation with grainspawn:


Fruiting it self can be instigated in different ways. My personal favorite is to get a much consolidation as possible, and as soon that you see small fruits forming on top to fold the bag over tight, and make some incisions (2 or 3) on the sides. Folding the bag over tight will stop the fruiting at the top of the sub, and the incisions form the new sites of fruiting. This method results in the densest fruits, almost as if they are being pushed out under great pressure.  Example:


Another way of fruiting is just to cut small holes at the sites the fruiting is already starting to happen in the bag. This ensures successful pinning, but the fruits are often less dense and packed than when using the first method. Example:


Lastly one can also decide to just completely cut off the top of the bag and use the whole upper part of the sub for pinning. Also works, but again, looser and lighter fruits. Example:


8. Growing in jars
  • Easy, cheap and green
  • Kind of a bitch to clean
  • Use the above mentioned substrate

Although bags do fine, I kinda hate all the wasted plastic, and they are not for free. I found however that if one stuffs a normal 500 ml jar with filterlid full with wood bulk substrate (fill it up completely, we’re not going to shake and leaving empty space will reduce success), sterilizes it and inoculates it with LC the mushroom can be fruited straight from the jar. Just wait till full colonization, open the jars, and place them in your fruiting area, simple as that. Easy and highly effective IMO. These jars can after colonization also easily be stored in the fridge to fruit at a later point. I’ve had them doing fine after more than 2 months of dormancy.



9. Dowels and growing on logs
  • Grow Hericium on logs outdoors
  • Maple, beech, oak, elm and other hardwoods

I've managed to make dowels of Hericium. Simple wooden dowels from the hardware store where soaked for 24 hours, and strained. These where then sterilized in jars. I've tried both adding grain spawn and LC to the dowels to inoculate, and found that both work fine. inoculation took about 2 weeks, and the dowels where kept in the fridge until I inoculated this fresh beech log/branch and fresh birch log earlier this spring.
Picture of some dowels, that started fruiting in the jar already:

These dowels were still used even after the fruiting. Seems to be no issue.
Both logs where integrated in my new wine cap outdoor bed. Beech on the left and birch at the top:

I honestly have no clue about the successfulness of Hericium on logs outdoors, but was up for a small experiment.
Found later: Hericium does fine in outdoor logs and treestumps inoculated with dowels. Good wood types are maple, beech, oak, elm and other hardwoods. Performs less on “paperbark” trees such a birch.
Result this fall, after one summer:





10. Growing other ways

More of a “left-over” experiment, it might be of use for some ppl. Used a monotub outside of normal fruiting chamber. I did not care for it much. Fruits are a bit brownish and small though. I suspect the humidity is not high enough. Tuning such a set up might harvest better results.


I'm sure those buckets used for oysters will do just fine btw.


11. Fruiting conditions
  • Maintain high FAE and low CO2
  • >80% humidity

This mushroom likes high humidity and FAE. I use a greenhouse for fruiting where I keep the RH at 80% to 90% while constantly having a fan on for FAE. Small holes and openings in the greenhouse provide enough fresh air to be exchanged.

Paul Stamets gives the following parameters in his book Growing gourmet and medicinal mushrooms:

Spawn run:
Temperature: 21-24 celcius
Relative humidity: 90-100%
Primordia formation:
Temp: 18 – 24 celcius
Rel. Hum: 95 – 100%
FAE: 5 – 8 x/h
CO2: 500 – 700 ppm
Light: 500 – 1000 lux
Fruitbody development:
Temp: 18 – 24 celcius
Rel. Hum: 85 – 95%
FAE: 5 – 8 x /h
CO2: 500 – 1000 ppm
Light: 500 – 1000 lux
Duration: 4-5 days

I found that temperature is no big issue. As long as it is above 15 celcius fruiting will happen without any issue. Heating is in my opinion not necessary what so ever.
I use 6500K bulb in my greenhouse for lighting. Not sure how essential, but I’ve used this from the start and never thought of trying something else.
As long as there is enough FAE to keep CO2 levels low while maintaining high humidity you should be fine.The fruit can handle a drop in humidity for a short while, before turning pinkish to yellow-brown.
Too little fresh air/too much CO2 will result in small lumpy fruits.
I’ve heard it is recommended to lower the humidity (to ~80%) for about 12 hours before fruiting, to increase the shelf life of the fresh shrooms.


Sorry for the long read, hope it was somewhat useful!
I would love to hear about more experiences or see your nice grow pics!

Cheers!
-Wii


Edited by Wrightii (08/09/23 06:58 AM)


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InvisibleQM33
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Re: The Hericium Thread [Re: Wrightii]
    #27286258 - 04/29/21 08:04 AM (2 years, 8 months ago)

Cheers on your grows and thanks for the shout out!.

I really wish you had a co2 meter. I've never grown comb tooth.
And did you mean lower humidity to 60 before harvest?

So your growing in a tent? How big? His much fresh air are you giving it?
Monos and sgfc would work but need alot of compensation for FAE and Humiditu, misting  and fanning. Ideally you would have your tent set up to flux appropriately.  But with that said you could put a grocery bag over your block and treat it like a tub. All ot is is a microclimate.

Also,I've heard that all the medicinal studies are being done with herocium erinacius, so not to say that the relatives done contain similar medicinal properties, they probably do, but they are far much less researched.


Love the write up tho, even tho some of the teks aren't going to perform ideally, it shows there's more than one way to skin a cat.
And I like the full process tutorial aspect of it.
Might as well throw a recipe in there haha!
Cheers brotha!


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Invisibleseldom seen
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Re: The Hericium Thread [Re: Wrightii]
    #27286265 - 04/29/21 08:08 AM (2 years, 8 months ago)

This is awesome, thank you.


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InvisibleWrightii
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Re: The Hericium Thread [Re: QM33]
    #27286498 - 04/29/21 11:36 AM (2 years, 8 months ago)

This is my current set up, which I build kinda recently with growing panaeolus in mind:

Humidifier on the fridge blows mist in at the top of the tent. 2 fans blow the air sideways and downward. At the top of the tent at the left panel I've punctured 20 or so small holes. Same for the down left pannel. With CO2 being heavy and the fans blowing down thus creating positive pressure at the bottom and negative pressure at the top this should lead to enough fresh air intake and no stagnent air while maintaining high humidity. Thats my plan/hope at least.

For oysters and hericium this is quite overkill though. I always used to work with a similair tent but 3 times as wide. In the middle of the tent was the ultrasonic humidifier blowing constantly at low capacity and a bathroom fan blowing horizontally at on/off intervalls of 15 mins. The tarp of this tent left about a cm of "open" at the bottom, giving the heavy CO2 an easy way to escape. This setup kept the humidity at around 80-85% while facilitating a lot of (fresh) air movement.

As long as you manage to keep CO2 low, fresh air high and humidity high any set up should do fine.
What do you use to fruit them?


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The Official Hericium Thread


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OfflineTheMagicConch
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Re: The Hericium Thread [Re: seldom seen]
    #27286561 - 04/29/21 12:32 PM (2 years, 8 months ago)

This is great to see. I'm about to start giving more gourmet mushrooms a shot at cultivation so I'll definitely contribute to this when the time comes


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OfflineTheMagicConch
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Re: The Hericium Thread [Re: TheMagicConch]
    #27286573 - 04/29/21 12:44 PM (2 years, 8 months ago)

So is your idea for this thread to be kind of like the active woodlovers thread? Just sort of a place for people to document what they've done and show their progress etc for others to take from?


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InvisibleWrightii
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Re: The Hericium Thread [Re: TheMagicConch]
    #27286582 - 04/29/21 12:52 PM (2 years, 8 months ago)

Yes that exactly what I have in mind! :smile:
The gourmet part of shroomery could be much more active if you'd ask me, maybe threads like this can contribute. I could also imagine threads happen for other species of edibles:thumbup:
Maybe a cultivation picture of the day thread would also be nice! So many beautifull gourmets out there


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The Official Hericium Thread


Edited by Wrightii (04/29/21 01:06 PM)


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OfflineTheMagicConch
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Re: The Hericium Thread [Re: Wrightii]
    #27286658 - 04/29/21 02:09 PM (2 years, 8 months ago)

Yeah for sure. Well I'm hella excited to get some lions mane going, now just to find a culture around here...


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InvisibleQM33
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Re: The Hericium Thread [Re: TheMagicConch]
    #27286681 - 04/29/21 02:24 PM (2 years, 8 months ago)

Wait so are your fans actually pushing air in? Or pushing air that's already in it?
Those holes won't be enough intake. You either in a legit intake or exaust.

And the c02 will only settle if completely stagnant I'm pretty sure, otherwiseits a gas and will mix readily with what's around it, given you have no pockets that aren't aloud to circulate.
Are you only growing those? Oysters can give a good indication of c02 levels if you dont have a gauge, they get fuzzy feet. Chestnuts and pippins also give signs that are pretty easy to distinguish


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


Edited by QM33 (04/29/21 02:25 PM)


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Offlinetrubblesome
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Re: The Hericium Thread [Re: TheMagicConch] * 1
    #27286685 - 04/29/21 02:30 PM (2 years, 8 months ago)

howdy!

I just woke up my lion's mane culture again.



cloned from a commercial fruit, printed the clone, grew out the spores, selected my favorite/best fruiting fruit from my setup. now this is my culture. I fruit it in my outdoor tent when the forecast looks right.

I had to go through a bit of an ordeal to get mycelium to clone from, which included generating spawn on cardboard in a shoebox, and eventually forcing fruiting in there to produce a fruit to pull clean tissue from.

this is what happens to erinaceus in a tub/high CO2 environment. I wonder what corralloides would end up looking like!!



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InvisibleWrightii
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Re: The Hericium Thread [Re: QM33]
    #27286704 - 04/29/21 02:42 PM (2 years, 8 months ago)

ah alright. Yeah i have not done any grows in the new set up so still dialling it all in.
Maybe I'll go for an exhaust fan at the bottom then. And more holes at the top. Thanks for the tips!


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Re: The Hericium Thread [Re: Wrightii]
    #27287281 - 04/29/21 10:57 PM (2 years, 8 months ago)

Here's my current block growing out.  I love watching lion's mane grow.  It's a really beautiful mushroom - really calm in appearance.

Anyway, this is from a strain procured from a local seller, but who may have grown out a strain from Mossy Creek, I can't remember atm.



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Re: The Hericium Thread [Re: taku] * 2
    #27288361 - 04/30/21 09:10 PM (2 years, 8 months ago)

Love this one for how much character this particular mushroom had.



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Re: The Hericium Thread [Re: taku] * 1
    #27289047 - 05/01/21 11:08 AM (2 years, 8 months ago)

one of my faves.. cooked with vegan butter and garlic, salt pepper, man this taste like lobster.  one interesting thing i noticed, now I use a high nutrient substrate; that cured issues of pinning early in bags before fruiting conditions -for me.



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Re: The Hericium Thread [Re: trippleblack]
    #27289298 - 05/01/21 02:40 PM (2 years, 8 months ago)

hi guys - here's the scoop. I got this kit on etsy, i put it into fruiting conditions (shoe box for the first 2 weeks then switched to( 1 plastic bag(clear) 1 zip lock) both with holes. The process was started April 6. I'm being super patient since its my first with this, but I wonder, based on what you see here, how long till harvest ? It has been growing at its on pace, even over the past week+ when I switched to the 2 plastic hole filled bags. Pic was taken today. Soon as I can harvest I plan on a biopsy into liquid culture, then making my own. I have all materials locked and loaded, waiting for harvest day



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Re: The Hericium Thread [Re: DezGWest7]
    #27290124 - 05/02/21 08:39 AM (2 years, 8 months ago)

hard to say how long without really knowing the conditions.  from pinning, it would take about 1week to be ready to harvest in my RH controlled FC.


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Re: The Hericium Thread [Re: taku]
    #27292103 - 05/03/21 01:51 PM (2 years, 8 months ago)

I put some lions mane spores I was sent 11 years ago on agar yesterday :lol: hopefully something useful grows from them, it's my first time inoculating agar with anything, so it might just be a total flop! Either way I'm having fun.

Any recommended teks for a GH? I don't have one set up and I didn't see one in the stickies (Other than the automated one by Gr0wer, which is a little too complex for my liking).


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Re: The Hericium Thread [Re: ninja cat 09]
    #27292279 - 05/03/21 03:24 PM (2 years, 8 months ago)

If you want something basic you could do a shotgun fruiting chamber (sgfc). Search it up on the forums.

I'm not completely familiar with gr0wers, but I do a basic automated grow tent from vivosun (32x32x62). Could check that setup out here


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Re: The Hericium Thread [Re: taku]
    #27292318 - 05/03/21 03:44 PM (2 years, 8 months ago)

Thanks! Yeah, I've made SGFC before, and I understand the basic ideas around a martha/greenhouse, I'm just somewhat unclear on how I should handle the humidifier and fans so that humidity is distributed evenly, I'll probably look at a few teks before heading over to home depot and seeing what I can put together.


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InvisibleWrightii
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Re: The Hericium Thread [Re: ninja cat 09]
    #27293654 - 05/04/21 09:57 AM (2 years, 8 months ago)

Great to see people are enthausiastic about getting a Hericium thread going!
Ill try to update the post with new information regurarly and will keep on the search for nice links to other threads to add.

If you happen to know any nice links to threads that would help making the source of info more complete (this can also be hunting, cooking or whatever related as long as its Hericium I'd say), please feel absolutely free to post them here and I'll add them to the OP. 

Hopefully we can make the original post helpfull and info dense by linking to and or discribing all that is possible and has been tried :smile:


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InvisibleWrightii
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Re: The Hericium Thread [Re: Wrightii]
    #27304311 - 05/11/21 01:12 PM (2 years, 8 months ago)

Started a new experiment with lionsmane in jars.
I've had succes with jar fruiting before but never tried this substrate.
Filled 3 jars to the brim with 14:3:1 beech sawdust: wheatbran : gypsum that was brought to field capacity.

Sterilized for 90 mins and when cool all of them were injected with 10 ml LC each.
I think I'm going to incubate the jars on their sides, rolling them every now and then to prevent pooling. Wonder how this is going to work out, as shaking is not at all possible.
I'll update here!


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Re: The Hericium Thread [Re: Wrightii]
    #27304692 - 05/11/21 06:01 PM (2 years, 8 months ago)

How come you went with 10ml? I'm assuming those are qt jars. I do 1-2ml/qt jar of grain spawn, so am just curious.


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Re: The Hericium Thread [Re: taku]
    #27304702 - 05/11/21 06:10 PM (2 years, 8 months ago)

I just inoculated a few jars it will be my first lions mane . glad to see this thread


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Re: The Hericium Thread [Re: Wrightii]
    #27305693 - 05/12/21 12:05 PM (2 years, 8 months ago)

These cocktail drinking straws made out of wheat straw (https://www.staples.ca/products/2979952-en-greenlid-wheat-drinking-straws-55-500-pack) are great for getting LC innoc.points deeper in the sub. One straw down the middle. Colonization does take longer on bulk sub (vs. grain). More LC/container might speed things up a bit. I've been using about 50ml per 32 oz. container on sub that's slightly drier than field capacity. Seems to work so far for oyster, shiitake, chestnut. Suspect it would work well for a top fruiting hericium.


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Re: The Hericium Thread [Re: taku]
    #27307915 - 05/14/21 06:41 AM (2 years, 8 months ago)

Quote:

taku said:
How come you went with 10ml? I'm assuming those are qt jars. I do 1-2ml/qt jar of grain spawn, so am just curious.




Simply because I had this 30ml sterile syringe laying around :tongue:.
And since I had 3 jars thats 10 ml each. Less would probably have been fine too, but since I'm not particularly short on LC I just do whatever most of the time, as long as stuff doesn't get water logged.
Already showing some growth on day 2: :smile:


What is your reasoning behind using 1-2ml? Also just curious:grin:


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Re: The Hericium Thread [Re: Wrightii]
    #27327054 - 05/28/21 06:27 PM (2 years, 7 months ago)

Thanks for the great write up Wrightii


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Re: The Hericium Thread [Re: Wrightii]
    #27327220 - 05/28/21 08:42 PM (2 years, 7 months ago)

Quote:

Wrightii said:
What is your reasoning behind using 1-2ml? Also just curious:grin:




Then I get 5-10 jars per syringe and from that 5-10 blocks. I don't have a laminar flow hood so I buy my LC. Just trying to make it affordable.


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Re: The Hericium Thread [Re: taku]
    #27327469 - 05/29/21 02:56 AM (2 years, 7 months ago)

As for the jars, this doesn't seem like my preferred method so far :grin:

The jars are colonizing, but slowely. The substrate is way to dense to shake so I'll just have to wait it out


Also put a bit of substrate I had left over in a small plastic cup at the same date.
Opening it up shows this is also going slowely, and the fungus is already making small fruiting bodies.


As this substrate is so dense and energy-rich I wonder what fruiting is going to be like.
More updates later!


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Re: The Hericium Thread [Re: Wrightii]
    #27327701 - 05/29/21 08:58 AM (2 years, 7 months ago)

Oh I just realized you out substrate in those jars and that they are not grain spawn. Do you just have a filter on the lid?

In the future you could make a hole in the center of the sub, carefully PC so it doesn't collapse, then drop spawn down the hole to inoculate so growth is quick and goes outward. This how commercial bottle growing does it.


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Re: The Hericium Thread [Re: taku]
    #27330308 - 05/31/21 12:08 PM (2 years, 7 months ago)

Why not use a SAB taku? I bought my syringes, but I've been working in one for a bit and getting better with my technique.

As far as lion's mane goes, I've got this fully grown out plate, gonna take transfers from it and store it in the fridge as a "culture slant" of sorts, make actual culture slants from the resulting plates (never make slants from fully colonized plates, they may harbour contams), and then start some grain.



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Re: The Hericium Thread [Re: ninja cat 09]
    #27330508 - 05/31/21 03:41 PM (2 years, 7 months ago)

I suppose I could ninja cat. I'm interested in learning agar technique and LC work, but am still working on honing my fruiting conditions for nice looking mushrooms at the moment. That's the next step though  :sporedrop:  :sporedrop:


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Re: The Hericium Thread [Re: taku]
    #27330524 - 05/31/21 03:59 PM (2 years, 7 months ago)

I don't even have an FC yet :lol:

I've got most of what I need for a greenhourse (I think) but still haven't put it together. It's not my first go, but it's my first time growing something other than oysters and cubes, so we'll see how it goes on that end when we get there.


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Re: The Hericium Thread [Re: ninja cat 09]
    #27330651 - 05/31/21 06:32 PM (2 years, 7 months ago)

Best of luck man! Looking forward to some pics :sporedrop:


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Re: The Hericium Thread [Re: taku]
    #27464112 - 09/11/21 11:54 AM (2 years, 4 months ago)

Super fruit man! Any tips and or tricks you’ve picked up on how to persuade the block to yield such massive fruits? Timing? Temps? Nutrients? Etc? Do you let the block colonize with huge white spots before sending to fruit? Or do you send immediately after full colonization before primordial take hold? And what if I may ask is your sub ratio/ ingredients?


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Re: The Hericium Thread [Re: Shiro] * 1
    #27464336 - 09/11/21 03:05 PM (2 years, 4 months ago)

Soy hulls and hardwood will be the best sub for lions main.


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Re: The Hericium Thread [Re: Shiro]
    #27464838 - 09/11/21 11:01 PM (2 years, 4 months ago)

I like to send my lions mane directly to the fc after full colonization. If you let em sit they'll start fruiting on top in the air bubble. It's easier to harvest if you can get them to fruit out a slit in the side of the bag.


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Re: The Hericium Thread [Re: deadmandave]
    #27466756 - 09/13/21 12:17 PM (2 years, 4 months ago)

You have any pics of full colonization? I started another jar and I'm not sure if it's colonized or not :frown:

EDIT: Nevermind, just saw the OP :facepalm: :lol:


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Re: The Hericium Thread [Re: taku]
    #27487717 - 09/30/21 04:38 PM (2 years, 3 months ago)

You can make liquid culture in a SAB. Order 1 syringe, get some pp #5 lids for quart jars, put a SHIP and filtration port on them with RTV silicon, mix up your preferred liquid culture, I got best results with Karo and a pinch of LME, just a pinch, literally. Use distilled water, now you have 1 quart ready to pressure cook. Tin foil over the top. Pressure cook. Pull out your pressure cooker and immediately move to sterilized SAB. I use generous amounts of iso. And than real quick use your sterile syringe of LC you ordered and squirt 1 CC in the quart through the SHIP (self healing injection port). Put it on a stir plate on the lowest setting and let run for a few days. I got best growth removing from the stir plate and letting sit still for a few days before returning to stir plate for a few more days. Oh yea I forgot you’ll need to put a magnetic stir bar in your quart jar before pressure cooking, now you have a full quart of liquid culture. That you can use to continue inoculating more quart jars with. Also you still have 9 cc left of the original syringe you bought. Save money with more time and energy. But You’ll only need to order 1 syringe a year if you don’t run into contaminates. You could prolly even get away with 1/4 cc but the more the quicker the colonization.

You can also run culture plates in a SAB no problem. Just get a tall tub so you can pour and maneuver inside it. I’m seeing how far I can stretch my sterile technique in a SAB before building a flow hood. I’d say the only thing a flow hood is really necessary for is using grain spawn to colonize 5 pound bagged bulk substrate. And that’s not even necessary. Just a little hard to not touch bag with jar and also you can only really do 2-4 bags at a time in a large tote. But it’s worked for me without any contamination. I’m about to run 30 blocks at a time in my atmospheric steam sterilizer and I’ll have to keep loading and unloading my SAB with 4 blocks at a time. So kinda a pain in the arse but totally possible. You can skip the bag sealer so that you don’t have to remove open bags from sterilize SAB, just use zip ties to close bags up inside the SAB.

Balling on a budget.


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Re: The Official Hericium Thread [Re: Wrightii]
    #27555902 - 11/24/21 04:50 AM (2 years, 2 months ago)

Been absent on the webs (or this web) for a while, but some fun things happened in the meanwhile.
As I stated in the original post I made dowels from a Hericium Corraloides that I cloned in autumn last year. These dowels were plugged in to a fresh cut log of beech.
Although it was kinda to be expected to happen, I was still delighted to see fruits. Already after only one summer even. Really cool! Turns out this method works just fine.



Seeing these beautiful fruits sparked my enthausiasm to start a new grow again.
Upon checking my culture slants that I also made a year ago, I found they started fruiting in the test tube, while in the fridge. Quite funny and impressive.


Hope others are still growing these, or looking for them to clone even (its the season where I live).

Cheers!


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Re: The Official Hericium Thread [Re: Wrightii]
    #27556476 - 11/24/21 03:18 PM (2 years, 2 months ago)

Very cool! I just got a coral tooth culture, I'd love to get something like that going.


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Re: The Official Hericium Thread [Re: FrostyStipe]
    #27556634 - 11/24/21 05:58 PM (2 years, 2 months ago)

Hell yeah to the hericium thread! I'm about to start growing eranaceus in jars and had a question for everybody. Has anybody experimented with whether gravity affects the aesthetic development of the fruitbody? I was considering laying the jars on their side sticking out a little over the shelf so they would droop down, but Im doubtful it will actually matter too much vs. Topfruitung. I wouldn't expect that it would affect yields, just curious if anyone has experimented with whether it matters for the look of the fruit/direction of the teeth maybe.


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Re: The Official Hericium Thread [Re: Soviet Data]
    #27556861 - 11/24/21 09:33 PM (2 years, 2 months ago)

In my experience it doesn't matter a whole lot. These 2 jars of Corraloides where both fruited while laying on their sides:

Not a whole lot difference in the "normal" morphology. Although come to think of it almost all my fruits hang sideways already anyway:


One thing doesn't change, no matter how you fruit; when it matures those "hairs" that start forming always adhere to gravity


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Re: The Official Hericium Thread [Re: Wrightii]
    #27557041 - 11/25/21 01:58 AM (2 years, 2 months ago)

I just made my first Lions Mane fruiting blocks! I started with quart jars of millet(no prep, 1cup millet, 1/2cup water 1/8tsp gypsum) inoculated with LC from Amazon. Once colonized I added half a jar each to 5lb bags of supplemented hardwood(5cups hardwood fuel pellets, 1 1/4cup wheat bran, 1.4 liters water). Once these blocks are colonized, I plan on starting a new one every few days to get a staggered harvest. How many flushes can I expect from each block?


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Re: The Official Hericium Thread [Re: JXAllen]
    #27557062 - 11/25/21 02:26 AM (2 years, 2 months ago)

I always found it difficult to get lion's mane to flush again after the first, unless I thew it outside.  Then, usually WEEKS later some random fruits would pop up, but that's about it.  Maybe others have better luck.


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Re: The Official Hericium Thread [Re: Forrester]
    #27557064 - 11/25/21 02:30 AM (2 years, 2 months ago)

I never grew lions mane, but with Corraloides i usually got 2 or 3 flushes. I would just put them back in the fruiting chamber and give them some good mistings at the slits in the bag. Did take quite a while tho sometimes, and the first flush is often the most pronounced anyway.

Then again, I only know it from corraloides. Your milage may very well vary.


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Re: The Official Hericium Thread [Re: Wrightii]
    #27557067 - 11/25/21 02:35 AM (2 years, 2 months ago)

Hmmm... this seems like a lot of "stuff" for just one flush.... would it be better for me to make smaller blocks? Or would that just result in smaller mushrooms?


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Re: The Official Hericium Thread [Re: JXAllen]
    #27557072 - 11/25/21 02:48 AM (2 years, 2 months ago)

I myself would at this point not change the bags/blocks anymore. That's probably going to be a huge party for contams. Maybe (if the second flush doesnt come) you can try dunking them to hydrate and cold shock. No idea if that would help here, but it does for many other mushrooms


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Re: The Official Hericium Thread [Re: Wrightii]
    #27557082 - 11/25/21 03:11 AM (2 years, 2 months ago)

Oh no! Lol,  I didn't mean making these bags smaller. They are what they are now. What I get is what I get :smile:  I meant, going forward, if I'm only expecting one flush per block,  should I just make smaller blocks? What size blocks and what ratio of grain spawn to supplemented sawdust would you expect to yield around a pound of fresh mushroom per block? In guessing at that number too... is a pound per harvest a reasonable expectation?


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Re: The Official Hericium Thread [Re: JXAllen]
    #27557084 - 11/25/21 03:19 AM (2 years, 2 months ago)

Pfoe haha. I think Stamets said something like 500 gr / 5 pound of supplemented substrate (so about 5% efficient). If you'll make your next blocks smaller the fruits will consequently also be smaller. Large blocks with a single or two slits for fruiting can produce quite impressive fruits.


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The Official Hericium Thread


Edited by Wrightii (11/25/21 04:23 AM)


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Re: The Official Hericium Thread [Re: Wrightii]
    #27557499 - 11/25/21 10:58 AM (2 years, 2 months ago)

Quote:

Wrightii said:
Pfoe haha. I think Stamets said something like 500 gr / 5 pound of supplemented substrate (so about 5% efficient). If you'll make your next blocks smaller the fruits will consequently also be smaller. Large blocks with a single or two slits for fruiting can produce quite impressive fruits.




Your fruits will not be smaller but your flush will be.


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Re: The Official Hericium Thread [Re: Forrester]
    #27557744 - 11/25/21 03:33 PM (2 years, 2 months ago)

Quote:

Forrester said:
I always found it difficult to get lion's mane to flush again after the first, unless I thew it outside.  Then, usually WEEKS later some random fruits would pop up, but that's about it.  Maybe others have better luck.




I'm in the midst of my first time growing from spawn vs. a kit, but when I've done kits before (pre-colonized sawdust block in a bag) they've always done one or two more flushes on their own. I would just spritz a plastic bag draped over the block for humidity and the fruits didn't grow spines properly, which I've read is a symptom of low humidity. So maybe dropping humidity for a bit then raising it again after a flush could trigger it?

The southwest mushrooms guy on YouTube top fruits from his bags then cuts holes in other parts of the bag for additional fruiting and seems to get good results. Not sure about multiple flushes from jars though. Do jars lose more moisture than bags? I've heard of people adding water to jars to initiate second flushes.


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Re: The Official Hericium Thread [Re: Soviet Data]
    #27557748 - 11/25/21 03:37 PM (2 years, 2 months ago)

Yeah a dry out then rehydrate period works for most stuff, it's just with LM I think there's a longer wait between, and isn't usually worth taking up space in the FC for that long for me.


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Re: The Hericium Thread [Re: Forrester]
    #27587373 - 12/19/21 02:39 PM (2 years, 1 month ago)

My first time inoculating with grain spawn I was worried about contamination (working with a SAB) so I did 2 jars supplemented and 2 pure oak pellets. Seems like none are contaminated!

I wanted to share this cool disparity between the growth of the jars with wheat bran vs. those without. Supplemented jar is on the left. It is growing faster and more uniformly. Neat stuff! Especially interesting to me because of how inexact my measurements were. Didn't have a scale at the time and most recipes go with weight not volume, so I did 2 cups fuel pellets and a quarter cup bran per jar, which didn't seem like no 20%, but I was erring on the side of caution.



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Re: The Hericium Thread [Re: Soviet Data]
    #27587490 - 12/19/21 04:18 PM (2 years, 1 month ago)

nice. did you go with a center hole and fill with grain or lc?


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Re: The Hericium Thread [Re: taku]
    #27587496 - 12/19/21 04:22 PM (2 years, 1 month ago)

Those big jars were grain spawn into a center hole. I had a bad view of what I was doing in the SAB since the jars are so huge haha. It seemed like the grain clumped and piled at the top so I'm not sure if much if any went into the hole. Time will tell I guess. Could be that the hole just filled up with less grain than I thought it would take.


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Re: The Hericium Thread [Re: Soviet Data]
    #27587561 - 12/19/21 05:20 PM (2 years, 1 month ago)

How big of a gas exchange hole you got in those lids?


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Re: The Hericium Thread [Re: Forrester]
    #27587579 - 12/19/21 05:35 PM (2 years, 1 month ago)

Mayb an quarter inch or so. My thumbs are pretty skeletal. You can kinda see the outline of the hole here.


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Re: The Hericium Thread [Re: Soviet Data]
    #27587616 - 12/19/21 06:13 PM (2 years, 1 month ago)

In my experience these type of jars packed with a dense substrate like sawdust and wheatbran don't do all that well. Colonization took much much longer for me compared to jars with a courser sub like woodchips (not weeks but months, which I never saw before). The clumpy sub became impossible to shake loose to speed it all up a bit. Compared to jars with (small) woodchips with grainspawn it took longer, and the yield as far as my sampling goes did not differ a whole lot. One even stalled and contamed, which is something uncommon for me doing Hericium.
Also; cleaning these after is hell compared to a loser sub.
I don't want to discourage you tho! Just my personal experience. 
Maybe my inoculation method wasn't perfect, as I just injected LC without any straw in the middle. Let me know if your experience differs! Maybe my (just-try-something) TEK is just lacking.

Good luck!


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Re: The Hericium Thread [Re: Wrightii]
    #27587638 - 12/19/21 06:34 PM (2 years, 1 month ago)

Thanks, Wrightii. Don't worry, I'm not discouraged! I'll take any advice I can get! :smile: Currently I'm throwing as much shit at the wall with what equipment I have and seeing what sticks. I did LC into sawdust with rye berries a few weeks ago in quart jars, with a little pile of extra grain on top. Those are both about half colonized since Nov. 30th and had no wheat bran. I'm guessing if they keep going at the rate they are full colonization time will be 5-6 weeks. I might try that again but with the wheat bran, to see if I can decrease the colonization time.

I'm curious to see how these bigass jars play out. After I bought them I read about how fresh air exchange can be a problem with bigger jars, but all my quart jars were in use and I got more substrate than I can use in a timely manner. They're colonizing nicely for the time being at least.


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Re: The Hericium Thread [Re: Soviet Data]
    #27587701 - 12/19/21 07:46 PM (2 years, 1 month ago)

I did qt. Jars with a 1/4" hole and poly stuffing as a filter. Worked great and grew decent enough. Didn't have any issues cleaning them, just used a stick with a scrubber end, the type you use for a glass or a water bottle. Anyway, only did one batch to gain some experience with bottle method. Want to try again in the future though, liked it.


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Re: The Hericium Thread [Re: taku]
    #27587879 - 12/20/21 01:08 AM (2 years, 1 month ago)

Quote:

taku said:
I did qt. Jars with a 1/4" hole and poly stuffing as a filter. Worked great and grew decent enough. Didn't have any issues cleaning them, just used a stick with a scrubber end, the type you use for a glass or a water bottle. Anyway, only did one batch to gain some experience with bottle method. Want to try again in the future though, liked it.




Cool. Hopefully that size hole is big enough for the 2 qt jars I have growing. How'd you go about inoculating yours?


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Re: The Hericium Thread [Re: Soviet Data]
    #27587890 - 12/20/21 02:31 AM (2 years, 1 month ago)

That's why I asked cause the main thing I'd be worried about is gas exchange. 1/4" hole isn't much for that size of jar especially the closer the mycelium gets to the bottom. Hopefully you don't have that issue though.


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Re: The Hericium Thread [Re: Forrester]
    #27587893 - 12/20/21 02:48 AM (2 years, 1 month ago)

You think it'd be worth sterilizing a tool and stabbing a quick hole in the top and throwing on another filter, since the top is already colonized with a nice mat of mycelium? Or would the risk of contamination outweigh the benefits of better gas exchange?

Also for future reference, how many/what size holes would you say is enough for a 2 qt jar? I've seen people use tyvek where the whole top can breathe, but it doesn't seem worth how much it would dry out the substrate, especially in the winter.


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Offlinetaku
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Re: The Hericium Thread [Re: Soviet Data]
    #27587998 - 12/20/21 07:09 AM (2 years, 1 month ago)

I inoculated in an SAB. Just opened the jars, dumped in my spawn, closed. No contam, all went well. 

I have a flow hood now so would make that process much easier if I replicate my test.

If 1/4" worked for 1 qt., Maybe 1/2" for 2qt?

For now, just see how the 1/4" plays out. Better to know definitive things that may have gone wrong than have a wild variable - like popping the lid mid colonization - throw everything.


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Re: The Hericium Thread [Re: taku]
    #27588177 - 12/20/21 11:07 AM (2 years, 1 month ago)

That's what I figured. Well I'll post updates as it progresses. guess I'll know soon enough if it stalls out. :ooo:


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Re: The Hericium Thread [Re: taku]
    #27588470 - 12/20/21 03:34 PM (2 years, 1 month ago)

Quote:

taku said:
For now, just see how the 1/4" plays out. Better to know definitive things that may have gone wrong than have a wild variable - like popping the lid mid colonization - throw everything.




^^^that


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Re: The Hericium Thread [Re: Forrester]
    #27589594 - 12/21/21 03:04 PM (2 years, 1 month ago)

Just tossing in a picture from an older grow so I can find this thread again. I am on a second flush after letting a jar sit in the fridge for several months over the summer and have some more on agar now.



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Re: The Hericium Thread [Re: ReverendMyc]
    #27602540 - 01/01/22 02:40 PM (2 years, 27 days ago)

Ok the mycelium in this jar is going apeshit but I have a concern. Is this sort of moisture condensation looking stuff bacteria or is it normal? It doesn't look like bacteria to me, especially in the context it appeared. Every jar I have has started to have little spacklings of moisture when the mycelium started it get thick and bright white looking. Is this normal? Clear metabolites? Hard to believe it's bacteria up against such healthy mycelial growth. Thanks, and happy new year :smile:


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Re: The Hericium Thread [Re: Soviet Data]
    #27602921 - 01/01/22 08:24 PM (2 years, 26 days ago)

Looks normal to me.


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Re: The Hericium Thread [Re: SHROOMSISAY01]
    #27603062 - 01/01/22 10:42 PM (2 years, 26 days ago)

Thanks! Getting close to fruiting time. :cool:


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Re: The Hericium Thread [Re: Soviet Data]
    #27616025 - 01/12/22 10:28 AM (2 years, 16 days ago)

I wanted to say hi to everyone here. I've been reading and learning a lot. I started kind of backwards. I got a lions mane block in November 2021. First flush I was trying to go cheap and lazy so it was too dry. I added a fogger and the second flush was much prettier and tastier and now I've been working on the clones I took to agar.





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Re: The Hericium Thread [Re: Forrester]
    #27616135 - 01/12/22 12:08 PM (2 years, 16 days ago)

Quote:

Forrester said:
That's why I asked cause the main thing I'd be worried about is gas exchange. 1/4" hole isn't much for that size of jar especially the closer the mycelium gets to the bottom. Hopefully you don't have that issue though.




Well luckily they ended up colonizing just fine! They're in the FC now I'll post pics of the final product.

Hi, DMTFire! Those fruits looks great. I've grown fucked up warty lions mane from kits without enough humidity before too haha. At least they look dope :cool:


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Re: The Hericium Thread [Re: Soviet Data]
    #27616630 - 01/12/22 07:57 PM (2 years, 15 days ago)

Don't mist lion's mane that will cause it to turn brown.


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Re: The Hericium Thread [Re: SHROOMSISAY01]
    #27616661 - 01/12/22 08:36 PM (2 years, 15 days ago)

You're talking about spraying mist directly on it, right? Not ambient mist falling from a humidifier?

I have one primordia in my FC turning a little brown and I moved it away from the flap in the tent that I open (it's very not air tight so I was worried its drying out by the opening). They are growing out of jars laying on their sides.


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How many flushes from a 6lb block [Re: SHROOMSISAY01]
    #27617790 - 01/13/22 07:24 PM (2 years, 14 days ago)

How many does everyone usually get? I've just started having success with lions mane and have gotten 2-3 from the few blocks I have.  I was getting ready to toss a depleted one and thought I'd re-hydrate by soaking and then toss it back in the fruiting chamber.  Anyone have any experience with this?  Heard about doing it with oysters, but the time I tried I didn't had any success getting the substrate to absorb any water.  This time the block seemed to soak up plenty and regain most of the original weight.  I'm hoping for good things :grin:




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Re: How many flushes from a 6lb block [Re: father_fungi]
    #27617832 - 01/13/22 08:01 PM (2 years, 14 days ago)

I'm on my 3rd flush on these blocks. They're all at various stages of recovery/growth. I've been harvesting around 1/2lb per flush.


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Re: How many flushes from a 6lb block [Re: JXAllen]
    #27617929 - 01/13/22 10:08 PM (2 years, 14 days ago)

I do 2 rounds. After 2 the yield is too small to make it worth the space or potential for contam, at least for me.


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Re: How many flushes from a 6lb block [Re: taku]
    #27618209 - 01/14/22 08:03 AM (2 years, 14 days ago)

I'm getting very nice dense clusters now that I've increased my FAE. However, the tops of my mushrooms start to turn yellow before the teeth begin to elongate. I'm not sure if this is from it being too humid, too dry or something else. I've got them in a martha tent with a humidifier on a controller, currently set to 88rh. Temp is 65F.



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Re: How many flushes from a 6lb block [Re: JXAllen]
    #27619765 - 01/15/22 12:08 PM (2 years, 13 days ago)

So I got some jars stalling out. After initial primordia formation I dropped the RH from 95 to 89 (kicks on at 79). What usually causes stalling? There's no browning happening or anything. I cranked the humidifier back up since they were growing fine before. I have one tiny little primordia spining out similar to a coralloides, which I think is just due to a CO2 pocket in the jar mouth. I'm guessing it's a humidity issue rather than light, since primordia formed.


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Re: How many flushes from a 6lb block [Re: Soviet Data]
    #27630878 - 01/24/22 11:26 AM (2 years, 4 days ago)

Quote:

Southerner said:
Would y’all mind helping me figure out what’s going on with lions mane on agar? I’ve read a couple other threads where they experienced the same thing but I’m still having trouble getting it to grow on agar.

I’ve grown a few bags out great but when I take a piece off of one plate to another it is hit or miss. Some plates have been sitting for weeks with nothing visible. I’ve seen it take off on plates before and move it to the same agar recipe and nothing. It’s confusing the hell out of me. Even took a small piece to wheat which took off but as soon as I took a grain back to agar it stalled. Fuckin stumped.

Grain water at 2.4% agar. Indoor temps between 60-70 most of the time.




I accidentally posted this in another thread a little while ago and hijacked it — again, sorry about that. Wanted to post an update in case anyone else experiences this.

I switched my agar from hot soaked grain water using tap water to LME agar using distilled water and the lions mane is growing nicely again on agar. Still not as fast as oyster but now I’m able to use it routinely with the others. 1.5% LME and 2.5% agar in distilled water. Also sped up agar growth for Gymnopilus and Mexicana.

Guessing something in my well water was having an effect as I’ve used grain water from both oats and wheat with the same thing happening. May try hot soaking my grains in distilled water in the future to test it.


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Re: How many flushes from a 6lb block [Re: Southerner]
    #27635574 - 01/27/22 07:15 PM (2 years, 21 hours ago)

Just wanted to say that good fruits can be grown with just a plastic bag and cardboard on a dinner plate.



Here's a fruit coming through the filter patch.  It's on it's 2nd flush, I'm using for tinctures and to put into gel caps so I may take some off the top, probably should have top fruited first. :shrug:

I had one block fruit over 3 months. :shocked:



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Re: How many flushes from a 6lb block [Re: schmutzen] * 1
    #27635673 - 01/27/22 09:13 PM (2 years, 19 hours ago)

Nice! I recently had a filter patch mushroom as well :smile: I'd moved all my LM to one shelf and apparently folded one of the filter patches on the outside of the fold.



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Re: How many flushes from a 6lb block [Re: JXAllen] * 1
    #27635678 - 01/27/22 09:22 PM (2 years, 19 hours ago)

When i finally get back to gourmet's im thinking im going to try lm first. Just a cool looking mushroom.


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Re: The Official Hericium Thread [Re: Wrightii]
    #27635680 - 01/27/22 09:26 PM (2 years, 19 hours ago)



This jar got kinda dried out. I was attempting to side fruit it and I guess the ambient humidity wasn't good enough. RH said 85-95% but it started to dry out regardless.

I decided to attempt a rehydration with this and a couple others what suffered a similar fate (this time standing upright lol). Other ones look good and just went back in the FC, but this guy has this orange blotch.

Bacteria? Should I chuck it in the garden? It doesn't appear to have any of this crap on the top where the fruit would actually grow from, only down the side in the picture there.


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Re: How many flushes from a 6lb block [Re: AlvWoodz]
    #27635683 - 01/27/22 09:30 PM (2 years, 19 hours ago)

It's great, started from a clone.  Mine has been very fast, 6 weeks from inoculation of grain to harvest.  Forgiving as well, been doing open air agar and agar2grain.


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Re: How many flushes from a 6lb block [Re: schmutzen]
    #27635686 - 01/27/22 09:33 PM (2 years, 19 hours ago)

SD
Looks sus but don't take my werd, been at this less than one year.


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Re: How many flushes from a 6lb block [Re: schmutzen] * 1
    #27635691 - 01/27/22 09:39 PM (2 years, 19 hours ago)

Quote:

namaste said:
It's great, started from a clone.  Mine has been very fast, 6 weeks from inoculation of grain to harvest.  Forgiving as well, been doing open air agar and agar2grain.




Open air agar?! You're bold :lol:. Your fruits look great btw.

Quote:

namaste said:

SD
Looks sus but don't take my werd, been at this less than one year.




I think so too lol. Just not sure how much of a problem is in that location in the jar.


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Re: The Official Hericium Thread [Re: Soviet Data]
    #27635780 - 01/27/22 11:54 PM (2 years, 17 hours ago)

Quote:

Soviet Data said:


This jar got kinda dried out. I was attempting to side fruit it and I guess the ambient humidity wasn't good enough. RH said 85-95% but it started to dry out regardless.

I decided to attempt a rehydration with this and a couple others what suffered a similar fate (this time standing upright lol). Other ones look good and just went back in the FC, but this guy has this orange blotch.

Bacteria? Should I chuck it in the garden? It doesn't appear to have any of this crap on the top where the fruit would actually grow from, only down the side in the picture there.




I don't think that's LM mycelium. Way too dense. All of my jars start pinning when they are fully colonized and you can clearly see the difference.


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Re: The Official Hericium Thread [Re: JXAllen]
    #27635800 - 01/28/22 12:23 AM (2 years, 16 hours ago)

I can see why you'd say that but I think it's just a quirk of the culture I'm using. I have a jar in the FC right now that started pinning while the lid was still on that looks almost as dense as this one. After incubating slightly too long, I had a failed fruiting attempt as described above from laying on the side, and it's only gotten more dense and white since. It's also of note that the jar was kind of let to go a little too long before the first attempt. The mycelium was not that dense at first.

Edit: also maybe relevant, my jar in the pic is bran supplemented oak pellets inoculated with grain spawn.


Edited by Soviet Data (01/28/22 12:31 AM)


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Re: The Official Hericium Thread [Re: Soviet Data]
    #27636015 - 01/28/22 07:58 AM (2 years, 9 hours ago)

I agree. They do start off wispy on grain, but they can get nice and dense as they mature. This is the best image of a dense myc jar that I have from my first grow of LM.


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Re: The Official Hericium Thread [Re: ReverendMyc]
    #27636034 - 01/28/22 08:32 AM (2 years, 8 hours ago)

:threadmonitor:


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Re: The Official Hericium Thread [Re: ReverendMyc]
    #27636585 - 01/28/22 05:33 PM (1 year, 11 months ago)

Quote:

ReverendMyc said:
I agree. They do start off wispy on grain, but they can get nice and dense as they mature.




:whathesaid:


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Re: The Official Hericium Thread [Re: Forrester] * 1
    #27636598 - 01/28/22 05:57 PM (1 year, 11 months ago)



not the best culture but is what i have to work.


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Re: The Official Hericium Thread [Re: Soviet Data]
    #27636683 - 01/28/22 07:58 PM (1 year, 11 months ago)

Quote:

Soviet Data said:
I can see why you'd say that but I think it's just a quirk of the culture I'm using. I have a jar in the FC right now that started pinning while the lid was still on that looks almost as dense as this one. After incubating slightly too long, I had a failed fruiting attempt as described above from laying on the side, and it's only gotten more dense and white since. It's also of note that the jar was kind of let to go a little too long before the first attempt. The mycelium was not that dense at first.

Edit: also maybe relevant, my jar in the pic is bran supplemented oak pellets inoculated with grain spawn.




Ah, I wasn't aware these were fruiting blocks in jars. I thought that was grain spawn. I've never seen LM fruited that way. Do you have any pictures of one growing a mushroom? Still...none of my actively fruiting blocks are even close to that dense.. But, I'll defer to the more experienced growers in the tread.


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Re: The Official Hericium Thread [Re: JXAllen] * 1
    #27636777 - 01/28/22 09:24 PM (1 year, 11 months ago)



This guy was a little CO2 choked but it tasted great!


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Re: The Official Hericium Thread [Re: Soviet Data]
    #27636808 - 01/28/22 09:50 PM (1 year, 11 months ago)

Very cool! :smile: And no wasted plastic! How long have you been growing them this way? How many flushes do you get or how much weight do you expect to harvest from each jar? How much work is it to empty the jars after they're spent?


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Edited by JXAllen (01/28/22 09:51 PM)


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Re: The Official Hericium Thread [Re: JXAllen]
    #27636826 - 01/28/22 10:23 PM (1 year, 11 months ago)

Quote:

JXAllen said:
Very cool! :smile: And no wasted plastic! How long have you been growing them this way? How many flushes do you get or how much weight do you expect to harvest from each jar? How much work is it to empty the jars after they're spent?




Thanks! Yeah not using disposable plastic was what made me wanna try this route. I cast no judgement on using bags, but I'm trying to stay away as long as possible.

I don't have much experience doing this, I've grown mushrooms a lot over the years but only from kits until now. I just rehydrated a few jars and let them sit to recover for a few days. Went back in the FC yesterday. They all smell and look good though so I'm encouraged.

My yield from the first flush of my first jar (unsupplemented) was a BE of about 35% at 51 grams. Hoping to get that number higher with a second flush, but it's also notable that I'm a novice, it's not supplemented, and I'm using a cheap culture lol.

Emptying the jars is no problem (for now). I just stab at it with a butter knife over my compost and they're easy to wash out. Meyers mushrooms on YouTube experimented with jars and he had a good idea for emptying them rapidly: a power drill with a large kitchen spoon attached. My drill is an old turd though so I haven't bothered.

I have a strong feeling I'll burn out and switch to bags eventually, but my goal is to gradually size up what I'm doing with the jars and see how far I can push it.


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Re: The Official Hericium Thread [Re: Soviet Data]
    #27636981 - 01/29/22 02:31 AM (1 year, 11 months ago)

If you let that substrate fruit all that it will and then dry out enough, it might just be small enough to pop right out the opening of the jar, or close.


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Re: The Official Hericium Thread [Re: Forrester]
    #27653529 - 02/10/22 09:58 AM (1 year, 11 months ago)

Trying these in muda style pint jars. 9 days from inoculation of masters mix with colonized oats.


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Re: The Official Hericium Thread [Re: ReverendMyc]
    #27684368 - 03/05/22 09:22 PM (1 year, 10 months ago)

Does this lion's mane LC look OK? or is it gone? I can't tell if the blobs are mold or fruiting bodies. They appear white, I don't see any green. I could test it on agar, of course, but I just pulled it from the fridge to inoculate a couple jars I sterilized last night.



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Re: The Official Hericium Thread [Re: JXAllen]
    #27684528 - 03/06/22 02:34 AM (1 year, 10 months ago)

Find out on agar or find out on grain, we can't tell ya by looking :shrug:

Looks like I wouldn't wanna use it but it could be great, I can't remember what old LM LC does exactly


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Re: The Official Hericium Thread [Re: Forrester]
    #27684539 - 03/06/22 02:51 AM (1 year, 10 months ago)

That's what I figured. Better to use a petri dish or two than waste 4 jars of grain... I'll drop some on agar.


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Re: The Official Hericium Thread [Re: JXAllen]
    #27686893 - 03/08/22 12:31 AM (1 year, 10 months ago)

never grown LM
i got lc from friend and he told me LM can inject straight to substrate
hard wood pallets and bran 70/30
anyone done it with success?


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Re: The Official Hericium Thread [Re: AbuSharka]
    #27686930 - 03/08/22 01:53 AM (1 year, 10 months ago)

I assume some have had success doing it that way, but I've seen a lot on here asking why it failed.  LC really doesn't take well straight to sawdust without going on grain first, but some people get it to work sometimes :shrug:


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InvisibleSoviet Data
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Re: The Official Hericium Thread [Re: Forrester]
    #27686942 - 03/08/22 02:16 AM (1 year, 10 months ago)

I did 10 ml of LC into sawdust my first time starting from culture. Despite not contaminating and actually eating a mushroom from one of them today, the yield was shit and it took MONTHS to colonize a quart jar lol - I wanna say I noc'ed it in early november. I probably coulda fruited it a little earlier... But ultimately don't think that woulda mattered too much.

I was running straight sawdust though. Also I was growing in a jar and definitely packed it a little too tight on that run. It might jump off faster with the bran, who knows. :confused:


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Re: The Official Hericium Thread [Re: Soviet Data]
    #27686990 - 03/08/22 04:56 AM (1 year, 10 months ago)

Quote:

Forrester said:
I assume some have had success doing it that way, but I've seen a lot on here asking why it failed.  LC really doesn't take well straight to sawdust without going on grain first, but some people get it to work sometimes :shrug:



might be the bran make going straight substrate easier and faster (then just sawdust)
I'll try it with different sawdust:bran ratio


Quote:


Soviet Data said:


I did 10 ml of LC into sawdust my first time starting from culture. Despite not contaminating and actually eating a mushroom from one of them today, the yield was shit and it took MONTHS to colonize a quart jar lol - I wanna say I noc'ed it in early november. I probably coulda fruited it a little earlier... But ultimately don't think that woulda mattered too much.

I was running straight sawdust though. Also I was growing in a jar and definitely packed it a little too tight on that run. It might jump off faster with the bran, who knows. :confused:



yeah my thought was bran make it easier go straight sub
it also means more nutrients so need to PC The substrate instead of pasteurized

I'll update in few months:awelol:


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Re: The Official Hericium Thread [Re: AbuSharka] * 1
    #27687765 - 03/08/22 04:13 PM (1 year, 10 months ago)

LM is very aggressive and fast on grain.  It can fully colonize a quart jar in 2 weeks.  Spawn to 5lb block of sawdust, no bran ~1 month.  So from dropping agar to wheat, I'm harvesting consistently within 6 weeks.


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Re: The Official Hericium Thread [Re: Wrightii]
    #28426746 - 08/09/23 07:10 AM (5 months, 17 days ago)

Browsing through the edible forum part this still seems relevant.
I restructured it a bit, hope I can help someone out.


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The Official Hericium Thread


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InvisibleFloret
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Re: The Official Hericium Thread [Re: ReverendMyc]
    #28496078 - 10/08/23 01:43 AM (3 months, 19 days ago)

Quote:

ReverendMyc said:
Trying these in muda style pint jars. 9 days from inoculation of masters mix with colonized oats.





Rev, how did this go?


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InvisibleRobert Bobsky
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Re: The Official Hericium Thread [Re: Floret]
    #28510246 - 10/19/23 05:58 AM (3 months, 8 days ago)

Hi all,

I see that Master's Mix is made with 50/50 mix of soy hulls and HW pellets.
My local tractor store doesnt have soy hulls but has soy flour as animal feed.
Do you think it could work as a supplement? Maybe not in the 50/50 ratio..

Thanks!


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Re: The Official Hericium Thread [Re: Robert Bobsky]
    #28510321 - 10/19/23 08:48 AM (3 months, 8 days ago)

Use any kind of bran, beet pulp, alfalfa pellets etc. Soy flour would probably work but you'll have to find the sweet spot. 20% is usually about right with most supplements.


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Re: The Official Hericium Thread [Re: deadmandave]
    #28522311 - 10/29/23 12:42 PM (2 months, 29 days ago)

This is the third flush of this block, and I've left it for longer than I usually do in an attempt to collect spores. It's growing teeth:


I plan to swab and streak on agar. Is that a good idea? What should I look for when spores are ready?


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Re: The Official Hericium Thread [Re: Bismillah]
    #28525297 - 11/01/23 09:21 AM (2 months, 26 days ago)

I have just cloned a wild Hericium abietis from southern oregon.

The culture is looking clean, but has not yet been tested in cultivation.

It appears that there are not many strains of Hericium abietis in circulation, and some of them are just mislabeled cultures of Hericium erinaceus.

I made a post in the marketplace about this culture:
https://www.shroomery.org/forums/showflat.php/Number/28525293


Is anyone interested in this culture?


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Re: The Official Hericium Thread [Re: Generic]
    #28526655 - 11/02/23 02:04 PM (2 months, 25 days ago)

Hey,

I'm starting to grow Lions Mane in my second FC and so far I've been growing only sporeless oysters so I haven't had to think about the spores at all.

If I'm growing 25lb of LM weekly for a long time, do I need a mask when working inside the FC? Of course I'll try to pick them in the prime time before they'll drop them all, but I know this will not be the case every time. At what stage they drop the spores? I suppose later than the prime picking time which should be when the teeth are just starting to form being just a few millimeters long?

I guess more cleaning will be involved too. So far I've haven't really had to clean that much, it's been almost too easy with the cleaning.


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Re: The Official Hericium Thread [Re: spasm666]
    #28527348 - 11/03/23 01:05 AM (2 months, 24 days ago)

I'd say you have ample time if your variety is similar to mine. It's been going 2 weeks past harvest time and it started growing balls on it's teeth. No spores as far as I can tell:



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Re: The Official Hericium Thread [Re: Bismillah]
    #28529236 - 11/04/23 03:32 PM (2 months, 23 days ago)

Put a piece of black paper under the fruiting body. I bet you will see spores within 24 hrs.


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Re: The Official Hericium Thread [Re: deadmandave]
    #28529879 - 11/05/23 05:06 AM (2 months, 22 days ago)

Oh yeah? So those balls drop spores you think? I've been swabbing a few times and got some nice bacterial growth. Also stuck a ball in a closed box hanging over foil. Swabbed the foil and grew some more bacteria..


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Re: The Official Hericium Thread [Re: Bismillah]
    #28531111 - 11/05/23 07:56 PM (2 months, 21 days ago)

Quick question for you long-time Hericium growers... This is my first time growing H. americanum, and my first time doing anything from spore I've collected. 

Was able to get one really good clean plate after a couple tries - swabbed with spores on 10/18, visible growth after 8-10 days, here it is today:




Since I've never done anything from spore before I'm wondering what is the thought process for selecting out samples to isolate for growing out/scaling up?



To my untrained eye, the mycelium circled in red looks weak and disorganized/fragmented while the 6 areas in blue look the strongest and most coherent and would be the ones I'd pick first for samples.  Does this make sense or would you sample a different area of the plate?


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Deep roots are not reached by the frost.

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Re: The Official Hericium Thread [Re: Bismillah]
    #28535507 - 11/09/23 11:13 AM (2 months, 18 days ago)

Quote:

Bismillah said:
I'd say you have ample time if your variety is similar to mine. It's been going 2 weeks past harvest time and it started growing balls on it's teeth. No spores as far as I can tell:







Wow I must say it's a bit hard to belive that no spores would have dropped with teeth so long. Maybe air currents took them away from you to see? Shouldn't they release the spores much earlier in general? Whether in this case spores dropped or not, I'd be happy to hear some other opinions. Thanks.


Edited by spasm666 (11/09/23 11:14 AM)


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Re: The Official Hericium Thread [Re: Bismillah] * 1
    #28536084 - 11/09/23 06:06 PM (2 months, 17 days ago)

Quote:

Bismillah said:
Oh yeah? So those balls drop spores you think? I've been swabbing a few times and got some nice bacterial growth. Also stuck a ball in a closed box hanging over foil. Swabbed the foil and grew some more bacteria..





The spores drop from the teeth like spasm said. Probably getting bacterial as those fruits are old and starting to decay.


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Re: The Official Hericium Thread [Re: deadmandave]
    #28541395 - 11/13/23 11:48 AM (2 months, 14 days ago)

Ok, I got a couple of probably a bit stupid questions about lions mane.

1. If I'm going to make my own LM tincture, I should make a double extraction to get all the good stuff, right? So I'll boil some dried fruit and make an alcohol extraction from that, if I've read it right.

2. I should be using fruitbodies AND mycelium to get all the good stuff, right? Fruitbodies I can do by how the hell I'm going to get mycelium? I mean just mycelium. If it's spawn I got grains there. If it's on agar, I got agar there. If it's a substrate I got the sub there. How I'm going to do this actually?

3. I fruited a bunch of LM with too low FAE and I got a big amount of these sponge-like marshmellowy balls. Is this the mycelium that I should do extract with? This the only way I can think of getting just mycelium without anything else along it. Or are these considered fruitbodies? They smell different than the actual nice fruits with teeth, the texture is different and they don't develop teeth so I think maybe it's mycelium.

Dunno if any of this makes sense.


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Re: The Official Hericium Thread [Re: spasm666]
    #28541494 - 11/13/23 01:25 PM (2 months, 14 days ago)

Probably would have to do microscopy or chemical analysis to determine if they're fruits or mycelium but most likely fruiting bodies.

What the big wigs report to do is colonize millet for so long that there's no millet left, only mycelium.

Double extract is going to gather more things than just boiling but will also act as a preservative so the concoction is shelf stable.


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Re: The Official Hericium Thread [Re: deadmandave]
    #28542539 - 11/14/23 10:58 AM (2 months, 13 days ago)

Right. Interesting, I wonder what the colonization time for that would be. Wasn't it the mycelium and not the fruitbodies that contains the best stuff? The stuff that helps with memory and other cognitive functions, can't remember what is was lol


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Re: The Official Hericium Thread [Re: spasm666]
    #28556735 - 11/26/23 05:45 AM (2 months, 1 day ago)

Sorry if this is a dumb question but do my substrate bags need to have fae during colonisation? Having a go at lionsmane but can't seem to find the answer anywhere.

Edit: that was a dumb question. Should've read the thread better and looked at the pictures.


Edited by HappinessStan (11/26/23 05:48 AM)


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Re: The Official Hericium Thread [Re: HappinessStan]
    #28571422 - 12/06/23 11:23 AM (1 month, 22 days ago)

Love this thread, will keep reading until I get my setup running then ill start contributing.

Keep asking questions and sharing everyone. Its how we all learn!


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Re: The Official Hericium Thread [Re: spasm666]
    #28571575 - 12/06/23 01:38 PM (1 month, 22 days ago)

Just thinking out loud without knowing the answer, but I feel like it would make sense to liquid culture if you just wanted to extract myc. Let it get extra chunky, filter out the solids, then either blend it up and start extracting or dry it up and grind it.

No idea about how the difference in compounds is between fruiting body and myc. I have noticeable improvements in concentration and brain fogginess using 3  mL/day of my fruiting body double extract. 60g bone dry fruit blended fine, 200 mL high proof grain alcohol, 2 weeks with daily shaking, filter, simmer solids for 4 hours with enough water for the final extract to be 30% alc.


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