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InvisibleWrightii
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Registered: 05/15/20
Posts: 216
Re: The Hericium Thread [Re: Wrightii]
    #27304311 - 05/11/21 01:12 PM (2 years, 8 months ago)

Started a new experiment with lionsmane in jars.
I've had succes with jar fruiting before but never tried this substrate.
Filled 3 jars to the brim with 14:3:1 beech sawdust: wheatbran : gypsum that was brought to field capacity.

Sterilized for 90 mins and when cool all of them were injected with 10 ml LC each.
I think I'm going to incubate the jars on their sides, rolling them every now and then to prevent pooling. Wonder how this is going to work out, as shaking is not at all possible.
I'll update here!


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The Official Hericium Thread


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Offlinetaku
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Re: The Hericium Thread [Re: Wrightii]
    #27304692 - 05/11/21 06:01 PM (2 years, 8 months ago)

How come you went with 10ml? I'm assuming those are qt jars. I do 1-2ml/qt jar of grain spawn, so am just curious.


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InvisibleSamushlove
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Re: The Hericium Thread [Re: taku]
    #27304702 - 05/11/21 06:10 PM (2 years, 8 months ago)

I just inoculated a few jars it will be my first lions mane . glad to see this thread


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Invisiblejunior c
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Re: The Hericium Thread [Re: Wrightii]
    #27305693 - 05/12/21 12:05 PM (2 years, 8 months ago)

These cocktail drinking straws made out of wheat straw (https://www.staples.ca/products/2979952-en-greenlid-wheat-drinking-straws-55-500-pack) are great for getting LC innoc.points deeper in the sub. One straw down the middle. Colonization does take longer on bulk sub (vs. grain). More LC/container might speed things up a bit. I've been using about 50ml per 32 oz. container on sub that's slightly drier than field capacity. Seems to work so far for oyster, shiitake, chestnut. Suspect it would work well for a top fruiting hericium.


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"Existence has overpowered books. Today, I slew a Mushroom" - Emily D


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InvisibleWrightii
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Re: The Hericium Thread [Re: taku]
    #27307915 - 05/14/21 06:41 AM (2 years, 8 months ago)

Quote:

taku said:
How come you went with 10ml? I'm assuming those are qt jars. I do 1-2ml/qt jar of grain spawn, so am just curious.




Simply because I had this 30ml sterile syringe laying around :tongue:.
And since I had 3 jars thats 10 ml each. Less would probably have been fine too, but since I'm not particularly short on LC I just do whatever most of the time, as long as stuff doesn't get water logged.
Already showing some growth on day 2: :smile:


What is your reasoning behind using 1-2ml? Also just curious:grin:


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The Official Hericium Thread


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Offlinedrbobgoatse
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Re: The Hericium Thread [Re: Wrightii]
    #27327054 - 05/28/21 06:27 PM (2 years, 7 months ago)

Thanks for the great write up Wrightii


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Offlinetaku
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Re: The Hericium Thread [Re: Wrightii]
    #27327220 - 05/28/21 08:42 PM (2 years, 7 months ago)

Quote:

Wrightii said:
What is your reasoning behind using 1-2ml? Also just curious:grin:




Then I get 5-10 jars per syringe and from that 5-10 blocks. I don't have a laminar flow hood so I buy my LC. Just trying to make it affordable.


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InvisibleWrightii
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Re: The Hericium Thread [Re: taku]
    #27327469 - 05/29/21 02:56 AM (2 years, 7 months ago)

As for the jars, this doesn't seem like my preferred method so far :grin:

The jars are colonizing, but slowely. The substrate is way to dense to shake so I'll just have to wait it out


Also put a bit of substrate I had left over in a small plastic cup at the same date.
Opening it up shows this is also going slowely, and the fungus is already making small fruiting bodies.


As this substrate is so dense and energy-rich I wonder what fruiting is going to be like.
More updates later!


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Offlinetaku
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Re: The Hericium Thread [Re: Wrightii]
    #27327701 - 05/29/21 08:58 AM (2 years, 7 months ago)

Oh I just realized you out substrate in those jars and that they are not grain spawn. Do you just have a filter on the lid?

In the future you could make a hole in the center of the sub, carefully PC so it doesn't collapse, then drop spawn down the hole to inoculate so growth is quick and goes outward. This how commercial bottle growing does it.


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Invisibleninja cat 09
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Re: The Hericium Thread [Re: taku]
    #27330308 - 05/31/21 12:08 PM (2 years, 7 months ago)

Why not use a SAB taku? I bought my syringes, but I've been working in one for a bit and getting better with my technique.

As far as lion's mane goes, I've got this fully grown out plate, gonna take transfers from it and store it in the fridge as a "culture slant" of sorts, make actual culture slants from the resulting plates (never make slants from fully colonized plates, they may harbour contams), and then start some grain.



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Offlinetaku
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Re: The Hericium Thread [Re: ninja cat 09]
    #27330508 - 05/31/21 03:41 PM (2 years, 7 months ago)

I suppose I could ninja cat. I'm interested in learning agar technique and LC work, but am still working on honing my fruiting conditions for nice looking mushrooms at the moment. That's the next step though  :sporedrop:  :sporedrop:


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Invisibleninja cat 09
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Re: The Hericium Thread [Re: taku]
    #27330524 - 05/31/21 03:59 PM (2 years, 7 months ago)

I don't even have an FC yet :lol:

I've got most of what I need for a greenhourse (I think) but still haven't put it together. It's not my first go, but it's my first time growing something other than oysters and cubes, so we'll see how it goes on that end when we get there.


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Offlinetaku
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Re: The Hericium Thread [Re: ninja cat 09]
    #27330651 - 05/31/21 06:32 PM (2 years, 7 months ago)

Best of luck man! Looking forward to some pics :sporedrop:


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OfflineShiro
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Re: The Hericium Thread [Re: taku]
    #27464112 - 09/11/21 11:54 AM (2 years, 4 months ago)

Super fruit man! Any tips and or tricks you’ve picked up on how to persuade the block to yield such massive fruits? Timing? Temps? Nutrients? Etc? Do you let the block colonize with huge white spots before sending to fruit? Or do you send immediately after full colonization before primordial take hold? And what if I may ask is your sub ratio/ ingredients?


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OfflineSHROOMSISAY01M
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Re: The Hericium Thread [Re: Shiro] * 1
    #27464336 - 09/11/21 03:05 PM (2 years, 4 months ago)

Soy hulls and hardwood will be the best sub for lions main.


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Offlinedeadmandave
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Re: The Hericium Thread [Re: Shiro]
    #27464838 - 09/11/21 11:01 PM (2 years, 4 months ago)

I like to send my lions mane directly to the fc after full colonization. If you let em sit they'll start fruiting on top in the air bubble. It's easier to harvest if you can get them to fruit out a slit in the side of the bag.


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Invisibleninja cat 09
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Re: The Hericium Thread [Re: deadmandave]
    #27466756 - 09/13/21 12:17 PM (2 years, 4 months ago)

You have any pics of full colonization? I started another jar and I'm not sure if it's colonized or not :frown:

EDIT: Nevermind, just saw the OP :facepalm: :lol:


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OfflineShiro
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Re: The Hericium Thread [Re: taku]
    #27487717 - 09/30/21 04:38 PM (2 years, 3 months ago)

You can make liquid culture in a SAB. Order 1 syringe, get some pp #5 lids for quart jars, put a SHIP and filtration port on them with RTV silicon, mix up your preferred liquid culture, I got best results with Karo and a pinch of LME, just a pinch, literally. Use distilled water, now you have 1 quart ready to pressure cook. Tin foil over the top. Pressure cook. Pull out your pressure cooker and immediately move to sterilized SAB. I use generous amounts of iso. And than real quick use your sterile syringe of LC you ordered and squirt 1 CC in the quart through the SHIP (self healing injection port). Put it on a stir plate on the lowest setting and let run for a few days. I got best growth removing from the stir plate and letting sit still for a few days before returning to stir plate for a few more days. Oh yea I forgot you’ll need to put a magnetic stir bar in your quart jar before pressure cooking, now you have a full quart of liquid culture. That you can use to continue inoculating more quart jars with. Also you still have 9 cc left of the original syringe you bought. Save money with more time and energy. But You’ll only need to order 1 syringe a year if you don’t run into contaminates. You could prolly even get away with 1/4 cc but the more the quicker the colonization.

You can also run culture plates in a SAB no problem. Just get a tall tub so you can pour and maneuver inside it. I’m seeing how far I can stretch my sterile technique in a SAB before building a flow hood. I’d say the only thing a flow hood is really necessary for is using grain spawn to colonize 5 pound bagged bulk substrate. And that’s not even necessary. Just a little hard to not touch bag with jar and also you can only really do 2-4 bags at a time in a large tote. But it’s worked for me without any contamination. I’m about to run 30 blocks at a time in my atmospheric steam sterilizer and I’ll have to keep loading and unloading my SAB with 4 blocks at a time. So kinda a pain in the arse but totally possible. You can skip the bag sealer so that you don’t have to remove open bags from sterilize SAB, just use zip ties to close bags up inside the SAB.

Balling on a budget.


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InvisibleWrightii
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Re: The Official Hericium Thread [Re: Wrightii]
    #27555902 - 11/24/21 04:50 AM (2 years, 2 months ago)

Been absent on the webs (or this web) for a while, but some fun things happened in the meanwhile.
As I stated in the original post I made dowels from a Hericium Corraloides that I cloned in autumn last year. These dowels were plugged in to a fresh cut log of beech.
Although it was kinda to be expected to happen, I was still delighted to see fruits. Already after only one summer even. Really cool! Turns out this method works just fine.



Seeing these beautiful fruits sparked my enthausiasm to start a new grow again.
Upon checking my culture slants that I also made a year ago, I found they started fruiting in the test tube, while in the fridge. Quite funny and impressive.


Hope others are still growing these, or looking for them to clone even (its the season where I live).

Cheers!


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OfflineFrostyStipe
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Re: The Official Hericium Thread [Re: Wrightii]
    #27556476 - 11/24/21 03:18 PM (2 years, 2 months ago)

Very cool! I just got a coral tooth culture, I'd love to get something like that going.


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