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Offlinegreggabott2
Registered: 10/26/20
Posts: 40
Loc: Canada
Last seen: 2 years, 8 months
Am I doing this LC thing right? * 1
    #27284549 - 04/28/21 01:24 AM (3 years, 8 months ago)

First attempt at LC. Put 2 grams of LME Into 500ML of water in a quart jar. Pressure cooked 15PSI for 30 minutes. Few hours later dropped a clean agar wedge into it. Does that sound right? How long should it take to fully colonize and how will I tell it’s ready to go into grains? Thanks


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InvisibleMilk-away
'Mmkay fortyseven
I'm a teapot User Gallery


Registered: 02/04/21
Posts: 249
Re: Am I doing this LC thing right? [Re: greggabott2]
    #27284624 - 04/28/21 04:03 AM (3 years, 8 months ago)

Yeah, looks good.
Make sure the polyfill is tight though and it doesn't get wet when you mix your liquid culture, bacteria can ruin a LC pretty quickly.

I saw mycelium strands forming in the liquid in 2-3 days, having usable liquid culture after a week

Good luck!


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...Let go...

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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Am I doing this LC thing right? [Re: Milk-away]
    #27284654 - 04/28/21 04:44 AM (3 years, 8 months ago)

I was under the impression that the standard mix for a liquid culture was identical to that of mea, being 20g lme to 1L water (10g lme to 500ml water). Seems like your mix might be a "tad" on the lean side.

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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 2 years, 7 months
Trusted Cultivator
Re: Am I doing this LC thing right? [Re: Feasoghorm] * 1
    #27284727 - 04/28/21 06:22 AM (3 years, 8 months ago)

Some like lean LC. I make them a tad stronger.

OP: Everything sounds correct. If you did it all right. I'd hate to look at an LC and see poly above it though. But that's just me. Don't trust the stuff. As mentioned above, you'll see growth soon and it will be usable soon. Let it go a little longer and you'll have more myc to work with.

I highly recommend testing LC on agar before committing it to a shitload of grain. It's as easy as flaming a loop, dipping it in the LC and streaking it across the agar. A few days later you'll know what your LC is made of.



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