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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Daddy4487]
    #27292754 - 05/03/21 07:25 PM (2 years, 8 months ago)

i cannot see any growth in the APE LC bag yet and its been 5 days. im wondering since i did not shake the syringe should i shake the syringe and re dose the bag with LC?


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InvisibleMilk-away
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Registered: 02/04/21
Posts: 249
Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27293251 - 05/04/21 01:13 AM (2 years, 8 months ago)

It can take some time, usually not this much time, but it depends on the conditions you have set up for growth.

Your temperature will affect colonization speed, I believe the optimal temp is between 21-26 degCelsius.
Citing from Radical Mycology, the book, mycelium might take a while to colonised when introduced to a new nutrient source, as it is selecting and fabricating the genes that will be best for consuming this type of nutrient. (so changing from honey to grains, it might put a little stress on the mycelium, but it should recover, unless you have many other stress vectors (contaminants, temperatures that are too hot/too cold etc).

You can always inject more Liquid Culture, since it is just live mycelium, but what do your grains look like? Have you inoculated a bag? Did you shake and roll the grains after inoculation to spread the LC all over the grains? If not, you can try to do that next time you inoculate.


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Milk-away]
    #27293255 - 05/04/21 01:26 AM (2 years, 8 months ago)

ok, my temps are at 22 degree`s atm, however early morning this would get down to 19 degree`s and slowly go up during the day to about 22 degree`s at night. What’s everyone think about my temps ? Do I need to up them a little ?

no i didnt shake and roll the bag ill do that next time, yes i did inoculate the bag. i think i may be able to see a little growth not sure ill confirm later tonight.

any more tips?  cheers


Edited by bubblegum (05/04/21 07:57 PM)


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27303510 - 05/10/21 08:27 PM (2 years, 8 months ago)




does this look all good or?


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InvisibleMilk-away
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27303758 - 05/11/21 02:48 AM (2 years, 8 months ago)

Oh sorry. I totally missed that it was an all in one bag, Shaking the bag wouldn't be a good idea.
Yeah, the growth looks good to me so far. I guess you just have to wait.
for my grains it can take up to 12-14 days for LC to fully colonize. Any more than that and you'd have to think about what could have gone wrong..


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Milk-away]
    #27304894 - 05/11/21 08:43 PM (2 years, 8 months ago)

ok, to fully colonize do u mean the complete bag turn white basically?


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27304897 - 05/11/21 08:44 PM (2 years, 8 months ago)

heres the pics from yesterday:

how much growth should i see in a day?











here is the APE it is a Liquid Culture at 11 days:









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InvisibleMilk-away
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27305006 - 05/11/21 10:57 PM (2 years, 8 months ago)

Judging from your pictures I'd say that your bags are going well (if slowly, but no other CONTAM issue).
Well you have to wait for the grains to fully colonize, before opening the bag, otherwise the whole thing turns to mold in a few days.
After your grains are white, the casing layer will colonize, some people put it straight into fruiting, other wait for the casing to get colonized, your choice really, but if the casing is nutrient rich, then I suggest to wait for it to get colonized, otherwise you can get mold.
When all is nice and colonized, you can open the bag, inspect for humidity(The surface of the casing must be covered in tiny tiny droplets, that glisten like diamonds or shattered glass when you shine a flash into them), and make sure the surface gets enough FAE to evaporate some of the water beads, which can induce pinning.
After that, just mist the surface and fan as much as you can/want/is needed.

God luck on your myco-journey!


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...Let go...


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OfflineLieutenant Pan
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Re: few beginner mistakes with all in one grow bag [Re: Milk-away] * 1
    #27305017 - 05/11/21 11:09 PM (2 years, 8 months ago)

Looks kinda wet in the bag should use 1CC.  It'll probably take 4 months to grow and it'll stall from bacteria before then.  Stop buying premade bags.


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Lieutenant Pan]
    #27305071 - 05/12/21 01:06 AM (2 years, 8 months ago)

half of them pics were the APE liquid culture and i only used 3cc. i don`t think it looks wet to me, i used too much on my first bags and that is the bag in the first few pics. looks very similar to the APE bag and the first pics of the bags were not LC. so will just have to wait and see.


Edited by bubblegum (05/12/21 01:08 AM)


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InvisibleWyoMX
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum] * 1
    #27305100 - 05/12/21 02:01 AM (2 years, 8 months ago)

I agree that pre-made bags pretty much stink I too tried to use a few when I first started had about 20% success rate. I never used the all in 1 bags, seems better to just get the grain bags colonized then mix with substrate. Those bags look fine to me for now though. I keep seeing more n more new growers getting liquid culture are people on reddit n other websites selling it? Not asking for a source I'm all set on spores and do agar, it just seems crazy to me people are selling LC since spores are already a hush hush operation.


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: WyoMX]
    #27305139 - 05/12/21 03:15 AM (2 years, 8 months ago)

yes people are selling liquid culture on websites.
the grower i get liquid culture of doesnt advertise it as liquid culture they just advertise it as a more expensive strain however when you message them and ask why its more expensive they then told me because its LC.


Edited by bubblegum (05/12/21 03:20 AM)


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OfflineDERRAYLD
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27305144 - 05/12/21 03:19 AM (2 years, 8 months ago)

Lc is sold by lots of vendors now, problem is it can spoil in transport due to the heat.


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: DERRAYLD]
    #27305147 - 05/12/21 03:24 AM (2 years, 8 months ago)

what temp will start to destroy LC? how long will LC survive in the fridge for? iv been told this is the best way to store it? and should last 6 months is this correct?


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OfflineDERRAYLD
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27305152 - 05/12/21 03:27 AM (2 years, 8 months ago)

Lc in cold is fine, lc that sits at a high temp for a while will spoil.


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: DERRAYLD]
    #27306487 - 05/13/21 01:18 AM (2 years, 8 months ago)

ok, are these bags similar to the all in one bags? i presume i can also fruit in this bag?

https://www.aussimushroomsupplies.com/index.php?route=product/product&product_id=170&search=PF+tek+bag


Edited by bubblegum (05/13/21 01:19 AM)


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OfflineRJ Tubs 202
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Re: few beginner mistakes with all in one grow bag [Re: Goatrider]
    #27306506 - 05/13/21 02:02 AM (2 years, 8 months ago)

Quote:

Goatrider said:

Spore syringes are usually loaded with some bacteria,
that`s why we never recommend to inoculate grains with.




It took me a long time to understand why my spore syringe to grain projects always crapped out!


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: RJ Tubs 202]
    #27306512 - 05/13/21 02:14 AM (2 years, 8 months ago)

Quote:

RJ Tubs 202 said:
Quote:

Goatrider said:

Spore syringes are usually loaded with some bacteria,
that`s why we never recommend to inoculate grains with.




It took me a long time to understand why my spore syringe to grain projects always crapped out!




this is why i am now only using liquid cultures. less chance off contamination!


Edited by bubblegum (05/13/21 02:15 AM)


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InvisibleGoatrider
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27306515 - 05/13/21 02:15 AM (2 years, 8 months ago)

You test that liquid cultures on agar?

          :cookiemonster:


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Goatrider]
    #27306519 - 05/13/21 02:21 AM (2 years, 8 months ago)

Quote:

Goatrider said:
You test that liquid cultures on agar?

          :cookiemonster:




haha, no! im yet to learn agar!
can you please reply to my post about the PF tek bags? im wondering can i fruit in them?


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