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Offlinebubblegum
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few beginner mistakes with all in one grow bag * 1
    #27283400 - 04/27/21 03:18 AM (2 years, 8 months ago)

Hello, everyone...Today i decided to start my first grow bag, its a all in one grow bag so i can do the full grow in the bag. I forgot a few simple steps, i heated the syringe up and juiced the bag but i forgot to sterilize the spot i injected on the bag and i also used the full 12ml spore syringe....have i stuffed up completely or should i be all good?


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InvisibleGoatrider
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27283422 - 04/27/21 04:54 AM (2 years, 8 months ago)

Welcome :sporedrop:

First of all, you inoculated in a sab?
Further you can just sanitize the spot with some alcohol, not sterilize.
And yeah 12ml is way too much, unless you have liquid culture.
But that`s another story.

It`s just a waiting game now, as

-you don`t know about substrate preparation quality
-you don`t know about spore quality

Grow bags are great, but better done G2G in front of a flowhood, for example.
Just sit this out now, and give some pictures in about 10 days.

                      :cookiemonster:


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Goatrider]
    #27283424 - 04/27/21 05:04 AM (2 years, 8 months ago)

thanks mate.

no i inoculated on my desk...ok cool no problem.

Is it alrite to sit the grow bags next to each other?


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InvisibleGoatrider
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27283428 - 04/27/21 05:14 AM (2 years, 8 months ago)

No problem just let them sit.
But don`t expect too much :wink:

In the meantime do some research here, every single link there is full of valuable information,
there`s really everything in you need.

Best step next is to move away from premade stuff :rockon:

              :cookiemonster:


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OfflineDaddy4487
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Re: few beginner mistakes with all in one grow bag [Re: Goatrider] * 2
    #27283433 - 04/27/21 05:23 AM (2 years, 8 months ago)

Yea bud that’s WAYYYY too much... I would bet 10-1 you’re gonna get contamination in your bag and it won’t be from injecting outside of your SAB.... MSS gave contam in them all of
Them do, try looking up agar and take that path it’s almost a guarantee if you only use clean cultures


--------------------
Hope I can help
          &
Thanks for the help!


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Daddy4487]
    #27283487 - 04/27/21 06:18 AM (2 years, 8 months ago)

why am i going to have contamination in the bags? too much spore liquid?


Edited by bubblegum (04/27/21 07:07 AM)


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InvisibleGoatrider
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27283956 - 04/27/21 02:12 PM (2 years, 8 months ago)

Spore syringes are usually loaded with some bacteria,
that`s why we never recommend to inoculate grains with.

It`s brf (pf tek) that works, cause brf can handle bacteria quite good.
Seems like bacteria gets collected in a corner there, and gets shut down for a while.

But yeah, the more spore liquid you shoot, the higher the risk of competitors.
A mighty weapon is agar, a medium in a petri dish where we put our spores,
to see what we get, and transfer good mycelium from there for further cleanup.
Usually we can see all the nasties that came with spore liquid,
cause they can`t hide on a 2D surface.
Finally we put that clean agar mycelium to grains, cause we`re sure now that it`s healthy.

In the end i won`t say that it never works, of course it can.
But chances are very low.
Don`t forget pics next week :wink:

          :cookiemonster:


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Goatrider]
    #27284320 - 04/27/21 08:36 PM (2 years, 8 months ago)

cool thanks mate. i wont forget the pics! i think from now on ill go with the agar method, whats the best thread on here to learn agar?  i have more spore syringes and i also have liquid culture arriving tomorrow :smile:


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InvisibleAshtray161
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27284345 - 04/27/21 08:47 PM (2 years, 8 months ago)

EDIT: DELETED.


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312


Edited by Ashtray161 (04/28/21 02:38 AM)


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Ashtray161]
    #27284412 - 04/27/21 10:04 PM (2 years, 8 months ago)

Quote:

bubblegum said:
thanks mate.

no i inoculated on my desk...ok cool no problem.

Is it alrite to sit the grow bags next to each other?



Quote:

Goatrider said:
Welcome :sporedrop:

First of all, you inoculated in a sab?
Further you can just sanitize the spot with some alcohol, not sterilize.
And yeah 12ml is way too much, unless you have liquid culture.
But that`s another story.

It`s just a waiting game now, as

-you don`t know about substrate preparation quality
-you don`t know about spore quality

Grow bags are great, but better done G2G in front of a flowhood, for example.
Just sit this out now, and give some pictures in about 10 days.

                      :cookiemonster:





hey Goatriders, what do u mean unless i have liquid culture?

as im getting liquid culture so im just wondering..


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InvisibleMilk-away
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum] * 1
    #27284420 - 04/27/21 10:15 PM (2 years, 8 months ago)

Hey bubblegum,

Welcome to the community.
Liquid culture means mycelium (the living organism of fungi), but in liquid form, so you can inject it in places (PF jars, Grain jars, Grain bags etc)
It is basically just a bit of mycelium (one cubic milimeter of mushroom or agar) that is left to cultivate in a nutritious solution (like 4% honey water).
After a while, the liquid will form a web of living mycelium and expand in the liquid jar.
When using liquid culture, you are making sure that you are not introducing contaminants when innoculating, because the liquid culture is sterilized prior to inoculation with mycelium, so if you are doing everything according to common sterile procedure, the contam vector would be a minimum.
Not only that, but since liquid culture is already live (dikaryotic) mycelium, you won't have to wait a week or two for the spores to "breed".

Good luck on your adventures


--------------------
...Let go...


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Milk-away]
    #27284445 - 04/27/21 10:35 PM (2 years, 8 months ago)

thanks mate, sounds very good thanks for the info. so how long after injecting liquid culture into a bag will i have to wait entill i fruit?

Does liquid culture require only a small amount to be used just like spore syringe?

Can i make more liquid culture from liquid culture?

cheers


Edited by bubblegum (04/27/21 11:00 PM)


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InvisibleMilk-away
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27284529 - 04/28/21 12:44 AM (2 years, 8 months ago)

Hi,

The liquid culture will have visible mycelium forming on your grain in 1-3 days, depending on the vigor and genetics of your LC.
You can use a small amount , like 1cc, as long as you transfer visible mycelium to your medium of choice, it will colonize. the more LC you inject, the faster it will colonize (since the mycelium will cover a greater surface area from the get-go), but increasing the moisture by a lot can sometimes increase the risk of developing bacterial contamination.
Yeah, you can propagate liquid culture almost indefinitely. The mycelium will lose some vigor after too many transfers, especially if you always transfer to the same food source(honey, potato etc), the fungi can become "lazy".
It's recommended to change the nutrition type once in a while (from honey to malt, to grain water etc).
But transfering from LC to LC is also risky, since you cannot see the contaminants that well on liquid, it is recommended to go from LC to agar, clean up any contams then to as many batches of LC as you want to make. this will pretty much ensure you are always working clean.

Also, building a Still Air Box is the best thing I ever did, it reduced my contam rate by like 98%.

Good luck!


--------------------
...Let go...


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Milk-away] * 1
    #27284542 - 04/28/21 01:09 AM (2 years, 8 months ago)

cool as, is 1cc = 1ml?

i will have a look at the Still Air Box`s.

what would this be like? https://www.amazon.com/Rogue-Spore-Mycology-Mushroom-Cloning/dp/B0821S812P

thank you...


Edited by bubblegum (04/28/21 01:16 AM)


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InvisibleMilk-away
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27284602 - 04/28/21 03:11 AM (2 years, 8 months ago)

Yeah, 1cc=1ml of liquid.

I don't recommend "Glove Boxes", so boxes that have gloves glued to the arm-holes, because that creates negative pressure and moves the particles around when you move your hands.
A still air box is arguably better at that. Basically just a 60l or bigger transparent tub with two holes cut in it for your hands.

This method allows air pressure to escape through the arm holes, thus the particles lying on the bottom will not be mixed with the air inside the box, making the air more "still"


--------------------
...Let go...


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InvisibleGoatrider
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Re: few beginner mistakes with all in one grow bag [Re: Milk-away]
    #27284606 - 04/28/21 03:26 AM (2 years, 8 months ago)

Look here for getting an idea mate:
https://www.shroomery.org/forums/showflat.php/Number/23990888

Look out not to buy a flat one, since you want to place some petri sleeves upright there.
Shouldn`t be below 40cm.
Armholes not too small, since you force piston effect or scratching around,
if holes are narrow.

      :cookiemonster:


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Goatrider] * 1
    #27286180 - 04/29/21 06:30 AM (2 years, 8 months ago)

ok thanks mate.

update on some progress!

Today i received a liquid culture of APE, so i decided to inject my 3rd bag. Todo this i decided to try and do it the correct way and i made up a flow hood i think its called, just a clear acrylic box with 2 arm holes. I tried to fully sanitize everything as best as possible with 70% alcohol wipes, the only thing i could have slipped up on was not shaking the syringe before use, should i have? also i used just under 3ml of liquid culture.


Edited by bubblegum (04/29/21 06:46 AM)


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OfflineAlaxandar
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Re: few beginner mistakes with all in one grow bag [Re: bubblegum]
    #27286462 - 04/29/21 11:09 AM (2 years, 8 months ago)

You made a Still Air Box, not a Flow Hood. If you followed the teks and everything is clean you should have some growth within 2-3 weeks with MSS, and a week with an LC! Good luck


--------------------
I merely roleplay a mushroom cultivator, I actually don't know anything, its all fake. :smirk:


Edited by Alaxandar (04/29/21 11:10 AM)


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OfflineDaddy4487
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Re: few beginner mistakes with all in one grow bag [Re: Alaxandar]
    #27286773 - 04/29/21 03:39 PM (2 years, 8 months ago)

Yes you should have shaken the syringe thoroughly not a huge deal... as far as prepping your SAB, I’ve always cleaned it with soap and water first then wiped down with 70% ISO... try putting a towel down either over ur lid to the tote or on the surface ur working on and mist the hell out of it with alcohol as well before putting ur SAB on top.... the damp towel will act like a flytrap to the contams, let it sit still for 5 minutes before working in it and make sure to move quickly and smoothly as not to stir up contamination and you should be golden..


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Hope I can help
          &
Thanks for the help!


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Offlinebubblegum
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Re: few beginner mistakes with all in one grow bag [Re: Daddy4487]
    #27287050 - 04/29/21 07:28 PM (2 years, 8 months ago)

ok no probs thanks heaps.


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