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0001001
πΚβ’Μα΄₯β’ΜΚπ



Registered: 02/04/21
Posts: 882
Loc: Inside an opium den
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Agar advice
#27283204 - 04/26/21 09:17 PM (3 years, 8 months ago) |
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How do these look? I have no experience and really don't know what sections to transfer or why. These are the agar plates I thought looked okay. Alot of them just turned out thin and whispy or fuzzy. I just want to get stable genetics cause im sick of having small fruit bodies.
Also, how does the oyster one look? I took it from a tissue sample of a fruit at the grocery store. It looks healthy but much different from the mycelium im used to.

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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,822
Last seen: 2 months, 5 days
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Re: Agar advice [Re: 0001001]
#27283322 - 04/27/21 12:05 AM (3 years, 8 months ago) |
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itβs kinda hard to tell whatβs going on from the pics, it looks to me like the plates are completely grown out to the edges?? iβd suggest dying your agar in the future, i know i find it much easier to see things that way. one of your plates looks like it has some decent rhizo growth, most of the rest looks ambiguously tomentose. you wanna take transfers much earlier if you can help it, and take them from anywhere along the leading edge of mycelium that looks uniform/is away from contamination. i have VERY limited experience with oyster myc (growing my first plates out rn) but i do know it looks different than cube myc. that plate doesnβt look horrible to me but like i said, hard to tell from pics
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0001001
πΚβ’Μα΄₯β’ΜΚπ



Registered: 02/04/21
Posts: 882
Loc: Inside an opium den
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The weird thing is... all my plates were colored red or blue..I guess the mycelium just consumed it π
What does that mean if they are ambiguously tomentose? I've never heard of that & didnt know to only take transfers from the edges. I'll try that next time though π
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,822
Last seen: 2 months, 5 days
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Re: Agar advice [Re: 0001001]
#27284487 - 04/27/21 11:43 PM (3 years, 8 months ago) |
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oh, tomentose is just how mycelium can look. itβs usually fluffier growth & can look sorta like a little cotton ball on the agar when it grows enough. rhizomorphic mycelium is the thick veiny mycelium you often see pictures of when people are showing off their agar/grains. neither is better or worse, rhizomorphic growth is just more aesthetic (& maybe easier to isolate from?) but some strains tend to be more tomentose than others in my experience. quite a few of your agar plates looked kinda tomentose to me. i didnβt really need to mention it, so iβm sorry if that was a little confusing for you!
also that happens to me when my agar plates have been running for awhile. the green dye i use turns a light shade of yellow as the mycelium uses it up
you wanna take from the leading edge since itβs the youngest mycelium & can make sure there are no contaminates on your transfer wedge. you can take transfers from anywhere on the plate as long as itβs clean, so if you do have a plate that you let sit for to long itβs OK to just take from closer to the center but generally the leading edge is preferred
-------------------- you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================
 
π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
Edited by hazyhorse (04/28/21 01:39 AM)
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