|
verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
|
Re: Cultivation General Discussion [Re: Crackatoa]
#27280396 - 04/24/21 12:31 PM (2 years, 10 months ago) |
|
|
Quote:
Crackatoa said: I would go with clone tissue over spore
Indeed.
|
LoadedFish
Psilocybin Enthusiast


Registered: 07/12/20
Posts: 107
|
|
They make it look so easy
|
starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 3 months, 18 days
|
Re: Cultivation General Discussion [Re: LoadedFish]
#27281254 - 04/25/21 08:30 AM (2 years, 10 months ago) |
|
|
Any of you got experience putting a single grain of colonized grain on agar? I got some mycelium I wasn't really expecting much of so of course I didn't keep a master culture.
To my brain there's no way it wouldn't work and any contams that hitch a ride can be transferred away from.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
|
hec
Stranger

Registered: 12/11/19
Posts: 4
Loc: St. Helena
Last seen: 2 years, 7 months
|
Re: Cultivation General Discussion [Re: starbones]
#27281262 - 04/25/21 08:37 AM (2 years, 10 months ago) |
|
|
this is a tub I currently have waiting to colonize, how do i know when its ready to be put into fruiting conditions?

|
Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 10 hours
|
Re: Cultivation General Discussion [Re: hec]
#27281265 - 04/25/21 08:41 AM (2 years, 10 months ago) |
|
|
Starbones, I've done that and it works quite well. I know others have as well. Just stab a candidate and stick it on a plate. Do beware loose grains will want to roll on firm agar. Either adjust or baby the plate for a couple days.
|
starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 3 months, 18 days
|
Re: Cultivation General Discussion [Re: Failboat]
#27281269 - 04/25/21 08:45 AM (2 years, 10 months ago) |
|
|
Bueno, thank you. I'll prepare some softer agar to get it buried nicely.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
|
Inthepit
Aum Mani Padme Hum


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 month, 14 days
|
Re: Cultivation General Discussion [Re: LoadedFish] 1
#27281348 - 04/25/21 09:52 AM (2 years, 10 months ago) |
|
|
Quote:
LoadedFish said: They make it look so easy 
Don't be misled, join LAGM next time and experience what really goes on behind the curtain!
|
verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
|
Re: Cultivation General Discussion [Re: Inthepit]
#27281380 - 04/25/21 10:20 AM (2 years, 10 months ago) |
|
|
HEC, Nice first post. That thing wants to breathe. Contrary to 1942's opinion, shit likes to breathe. You could come to any conclusion you want on when to apply "fruiting conditions" but I'd give her some air.
Starbones, Quirk is indeed correct. Just follow sterile procedure and everything will be fine. I'd just cut a little dig out in the middle as opposed to prepping a whole batch of soft agar. Or just dump some grain on and give no fucks cause you're just going to be transferring to a new plate any who.
|
smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,884
Last seen: 11 hours, 37 minutes
|
Re: Cultivation General Discussion [Re: starbones]
#27281422 - 04/25/21 11:07 AM (2 years, 10 months ago) |
|
|
Quote:
starbones said: Any of you got experience putting a single grain of colonized grain on agar? I got some mycelium I wasn't really expecting much of so of course I didn't keep a master culture.
To my brain there's no way it wouldn't work and any contams that hitch a ride can be transferred away from.
Done hundreds of times, works great
-------------------- Willpower is the one true virtue
  
|
Inthepit
Aum Mani Padme Hum


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 month, 14 days
|
|
Quote:
smalltalk_canceled said:
Quote:
starbones said: Any of you got experience putting a single grain of colonized grain on agar? I got some mycelium I wasn't really expecting much of so of course I didn't keep a master culture.
To my brain there's no way it wouldn't work and any contams that hitch a ride can be transferred away from.
Done hundreds of times, works great
Sorry, I'm confused, what does that accomplish? Wouldn't a clone be way more productive? It seems like you just end up with more myc, mebbe more isolated, but just myc.
|
smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,884
Last seen: 11 hours, 37 minutes
|
Re: Cultivation General Discussion [Re: Inthepit]
#27281484 - 04/25/21 12:08 PM (2 years, 10 months ago) |
|
|
Test jars? Save a culture you only have on grains?
-------------------- Willpower is the one true virtue
  
|
starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 3 months, 18 days
|
Re: Cultivation General Discussion [Re: Inthepit]
#27281491 - 04/25/21 12:15 PM (2 years, 10 months ago) |
|
|
Quote:
Inthepit said:
Quote:
smalltalk_canceled said:
Quote:
starbones said: Any of you got experience putting a single grain of colonized grain on agar? I got some mycelium I wasn't really expecting much of so of course I didn't keep a master culture.
To my brain there's no way it wouldn't work and any contams that hitch a ride can be transferred away from.
Done hundreds of times, works great
Sorry, I'm confused, what does that accomplish? Wouldn't a clone be way more productive? It seems like you just end up with more myc, mebbe more isolated, but just myc.
This is mycelium from a clone culture I neglected to save because I wasn't thinking the performance was going to be great so I was giving it a one and done. It colonizes like a motherfucker, faster than my Rustywhyte culture which is blazing fast already. Ergo I jumped the gun on not keeping the culture except for the grains it was put to. This will allow me to save the culture.
I could take a clone of the fruits but I never have good luck with clones of clones.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
|
Inthepit
Aum Mani Padme Hum


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 month, 14 days
|
Re: Cultivation General Discussion [Re: starbones]
#27281504 - 04/25/21 12:29 PM (2 years, 10 months ago) |
|
|
Thanks Starbones, that makes sense.
|
verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
|
Re: Cultivation General Discussion [Re: Inthepit]
#27281522 - 04/25/21 12:47 PM (2 years, 10 months ago) |
|
|
Taking a clone once it's grown out will only offer you an older culture. That's why it's good to save on agar in the first place. Definitely snatch a grain and get her save before all the cell division that happens while colonizing the sub.
If you are in a "fuck it" mood, you can literally just dump a few grains in like it's a g2g, wrap the sucker and throw her on the shelf. You'll have plenty of places to snatch up some myc and get her slanted or on a new plate.
|
BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 9 months, 7 days
|
Re: Cultivation General Discussion [Re: hec]
#27281633 - 04/25/21 03:00 PM (2 years, 10 months ago) |
|
|
Quote:
hec said: this is a tub I currently have waiting to colonize, how do i know when its ready to be put into fruiting conditions?
 
You can introduce fruiting conditions from day 1 of spawning.
|
Rebelutionsssss
Mdmazing



Registered: 07/23/14
Posts: 13,137
Loc: San Francisco
Last seen: 2 years, 3 months
|
Re: Cultivation General Discussion [Re: BrownBear]
#27281638 - 04/25/21 03:04 PM (2 years, 10 months ago) |
|
|
I usually leave my fae holes plugged up until it starts to pin so it doesn’t lose too much moister during colonization but a lot of people do it different. It’s up to you
-------------------- : To define is to confine.
|
verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
|
|
Both ways work but our enemies love stagnant moist conditions. Fresh air is a good thing. I spawn to tubs that are getting about 50% fresh air (compared to what I give them at "fruiting time") and then open it up to 100% once the pin set is in (for most cultures).
|
starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 3 months, 18 days
|
|
In my opinion fruiting conditions from spawning is the best method because as said we're dealing with shit like mold which wants warm, stagnant, moist air.
I noticed that employing fruiting at spawn combined with making sure grains are covered has let me reliably get to the third flush. Your mileage may vary. 1/4" or a little more of CVG and misting keeps the grains from dying out and becoming vulnerable in case your conditions are less than dialed. Dry winter was an absolute bastard for that and going back to covering my grains prevented a lot of bullshit when I couldn't reliably keep the surface conditions.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
|
Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 10 hours
|
Re: Cultivation General Discussion [Re: starbones]
#27281664 - 04/25/21 03:22 PM (2 years, 10 months ago) |
|
|
Unmodified tubs, dialed in, set n forget. I still peek tho
I might have to try modifying a couple tubs, but...
|
tututotutut
You...! You're not me!



Registered: 01/18/21
Posts: 2,218
Last seen: 2 years, 6 months
|
Re: Cultivation General Discussion [Re: Crackatoa]
#27281676 - 04/25/21 03:30 PM (2 years, 10 months ago) |
|
|
Quote:
Crackatoa said: I would go with clone tissue over spore
I had a lot of bad luck trying to do fruit-cloning, spread lots of bacteria! I feel more confident going spores to agar because then I feel like there's a higher chance of germination before contamination- also 1 spore print can be used more than one fruit for cloning. Maybe I should try different cloning teks
-------------------- yeah keep telling yourself that's voluntary
|
|