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zensane
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Help with a Liquid Culture Tool Name
#27277957 - 04/22/21 09:47 AM (3 years, 8 months ago) |
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I have recently started using liquid culture (LC) and everything is working as expected. Currently, I just pour the LC from the LC jar into the grain jars.
I want a syringe tool that I can use to inoculate grains in bulk so that I don't have to open my LC jars. The one I have in mind is a glass syringe with a ring for my thumb so I can pull liquid inoculate up via a self-healing port and inoculate more efficiently.
Or, maybe I am thinking totally wrong. Is there a better way?
Thanks!
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Vinci
I'm You



Registered: 08/26/19
Posts: 649
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Re: Help with a Liquid Culture Tool Name [Re: zensane]
#27277973 - 04/22/21 10:00 AM (3 years, 8 months ago) |
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Dosys 173
-------------------- Vinci luh you
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MLPismyOPSEC
That One Ponyfucker


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Re: Help with a Liquid Culture Tool Name [Re: Vinci]
#27278259 - 04/22/21 03:16 PM (3 years, 8 months ago) |
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Socorex refilling syringe is the term you're looking for, another member (verum subsequentis i believe) said he used one for LCs for a while and it was handy.
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sandman420
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Re: Help with a Liquid Culture Tool Name [Re: MLPismyOPSEC]
#27278384 - 04/22/21 05:40 PM (3 years, 8 months ago) |
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I used to use a socorex syringe for LC but they clog up with mycelium in the springs of the 2 ball valves if the LC is very thick. Then it's a whole thing.
Pouring is a better option imo.
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verum subsequentis
seeker of truth



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Re: Help with a Liquid Culture Tool Name [Re: sandman420] 1
#27278550 - 04/22/21 08:17 PM (3 years, 8 months ago) |
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I quit using it because it was an extra unnecessary step. It was handy enough but certainly not needed. I just pulled mine back out of storage and thought it might be fun to use again. Then thought..... Fuck it. I'll hang it on the wall as art.
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zensane
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Thanks for all the replies. Based on the replies, it seems pouring is best.
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verum subsequentis
seeker of truth



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Re: Help with a Liquid Culture Tool Name [Re: zensane]
#27279764 - 04/23/21 08:28 PM (3 years, 8 months ago) |
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When I got mine, it was because I was terrified that I'd accidently poop in my LC when I dipped into it to streak on agar as a test. With it, I had hoped to be able to noc it up and never open it up again and risk pooping in it. Problem is I'd have to use it within a few days of having pumped some through to test or it'd jam up. I could avoid this issue by tilting the jar and pumping air into the unit but that was just another thing to do and I fell out of love with it. Plus, I got confident enough in working in the flow that I have almost no fear of accidental pooping.
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blackout


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Evans
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Re: Help with a Liquid Culture Tool Name [Re: zensane]
#27280114 - 04/24/21 06:29 AM (3 years, 8 months ago) |
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I would just pour, but check out this liquid media extractor -
There is a link in the video description to purchase it if you're interested.
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Professor X
School for the Gifted



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Re: Help with a Liquid Culture Tool Name [Re: Evans]
#27280236 - 04/24/21 09:38 AM (3 years, 8 months ago) |
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Pouring is best. The only problem I had with pouring was where to set the lid between pours so I devised a jar lid with four tiny gauge nails sticking out of it and screw it on a pint jar then I sterilize the nails after setting it in the flow, afterwards I can transfer the LC lid back and forth freely as many Tamils as I want given I don't place my hand directly above the "lid stand". I have never contaminated one of my cultures using this technique.
Moving from agar to LC inoculation was scary at first but sooo rewarding. I recently moved to bags and omg. I can fit 20 jars worth in the PC at once for a total of 40 jars per load! Saves so much time and space. I actually got my closet back (for the most part, I still have some LC and agar up there but it's soon to move to a grow closet.)
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sandman420
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Re: Help with a Liquid Culture Tool Name [Re: Evans]
#27280255 - 04/24/21 09:54 AM (3 years, 8 months ago) |
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Quote:
Evans said: I would just pour, but check out this liquid media extractor -
There is a link in the video description to purchase it if you're interested.
what an interesting overcomplicated vector fest!
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Evans
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Re: Help with a Liquid Culture Tool Name [Re: sandman420]
#27280287 - 04/24/21 10:21 AM (3 years, 8 months ago) |
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Haha. It's what he's looking for so I figured I'd link it. Would be cool for some master LC.
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verum subsequentis
seeker of truth



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Re: Help with a Liquid Culture Tool Name [Re: Evans]
#27280384 - 04/24/21 12:16 PM (3 years, 8 months ago) |
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Quote:
overcomplicated vector fest!
My thoughts exactly. My favorite (one of) sentence applies here though...
You do you boo.
I'd have a shitload of masters nocced up via free pour before he could get his pipette attached to that dangly deal that's just begging to pick up something nasty.
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sandman420
Saint PP



Registered: 06/17/04
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The dangly bit bouncing off his glove made my eye twitch lol
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verum subsequentis
seeker of truth



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Re: Help with a Liquid Culture Tool Name [Re: sandman420]
#27280520 - 04/24/21 03:15 PM (3 years, 8 months ago) |
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Made my butthole pucker but we all react differently.
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Vinci
I'm You



Registered: 08/26/19
Posts: 649
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I'm a big fan of the LC gun. I made mine just like verum's. I shoot the bags up then seal with hot glue. Sometimes in my 2x2 FH pouring and resealing is cramped. The socorex method could work in a SAB even. I've had much better success with my socorex than pouring, but if I had a less cramped flowhood I'd probably go back to pouring. The valves clog up a little but with a few pumps they unclog themselves. Can recommend.
-------------------- Vinci luh you
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verum subsequentis
seeker of truth



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Re: Help with a Liquid Culture Tool Name [Re: Vinci]
#27280999 - 04/25/21 01:36 AM (3 years, 8 months ago) |
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I've had them jammed up worse than a few pumps would clear. I've had them jammed to the point that nothing was possible but pausing the session and dumping to clean.
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sandman420
Saint PP



Registered: 06/17/04
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And then i've had the same happen but then lost the fucking microscopic spring when trying to clean it then have to order a $15 parts kit lmao. I think I got all mad and broken the glass barrel and then threw it away when I saw that it was $100 for a barrel.
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verum subsequentis
seeker of truth



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Re: Help with a Liquid Culture Tool Name [Re: sandman420]
#27281396 - 04/25/21 10:38 AM (3 years, 8 months ago) |
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Ha. I lost that spring once too. Learned to clean carefully after the first time. Luckily mine came with a spare.
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Vinci
I'm You



Registered: 08/26/19
Posts: 649
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-------------------- Vinci luh you
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