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OfflineChemike
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Registered: 07/19/17
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Trying to grow; Tuber melanosporum and Tuber borchii and Tuber magnatum
    #27270447 - 03/26/21 05:13 PM (1 month, 9 days ago)

Hello,

I am doing an experiment. I believe, as with Amanita muscaria which I am also trying to grow, you need to add xylitol to the substrate for fruits to initiate. I did research and xylitol seems to be the common compound in the symbiotic relationship to the fungus and tree. Also muscimol and xylitol both have the five member carbon chain as if xylitol is used for production of those compounds. (In relation to Amanita muscaria). Unfortunately my Amanita m. isn't growing from spores on my petri dishes, but I have good news and my Tuber borchii is growing on all plates, from a tissue piece I placed on each. I am also doing the same with with my black truffles I got today. I have a bunch of pictures to show. The picture with the sprouts supposedly contains white truffle mycelium and the growth around the seed looks similar to the Tuber borchii mycelium. Very exciting !

I will be eventually inoculating rye grain with my 4% honey solution that contains the mycelium (that is what the picture shows-ready for sterilization in my autoclave/pressure cooker!) The rye grain contains and was prepared with 0.5% xylitol and a small amount of some yeast nutrient added. Hopefully the experiment goes well and my hypothesis is correct. I also added rockwood cubes in the centre of the rye grain bag to soak up the solution of 0.5%xylitol and can inject more sterile solution in the area to get sucked up by it if needed.





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Edited by Chemike (03/26/21 05:18 PM)


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OfflineAlan RockefellerM
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Re: Trying to grow; Tuber melanosporum and Tuber borchii and Tuber magnatum [Re: Chemike]
    #27273852 - 03/29/21 01:47 AM (1 month, 7 days ago)

Amanita muscaria spores don't germinate on agar - you need to clone a fresh fruit to get it into culture.

If it's really slow you likely have the right fungus growing.  I forget if it's 1 cm per week or 1 cm per month, but it's one of the two.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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