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OfflineTwoOneFiveGold
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Registered: 03/04/21
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Recognizing and dealing with contamination [Re: Kizzle]
    #27237139 - 03/04/21 02:35 PM (3 years, 25 days ago)

https://https://https://https://Bare with me I'm new to forum's and mushrooms lol but I have a possible problem with my first go at fungus and not sure what's going wrong if anything. Everything went perfect up to fruiting. So trying to keep this short, I inoculated grain spawn with online syringe that took about two weeks but great smell went perfect with no contaminate. The I bulk spawned into coco/perlite/vermiculite substrate into 5 quart about 2-3 inch deep, then covered and let sit 10 day to two weeks. Opened two of them up (did 5 total atm) at 10 days and they looked perfect nothing weird other then what seemed to be a clear film over some parts and seemed a bit wet, after a day or two I noticed some discoloration and tossed both. Cleaned everything top to bottom them opened up then other three at day 15 and 3 days in I'm not sure if its contaminant or bruising after some research and still worried. And comments or help would be extremely appreciated. Thanks all I'm gonna try and put pics up but cam is iffy at best, and dry for long post if I should be somewhere else posting please help me please and thanks 


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OfflineProfessor X
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Registered: 04/18/19
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Re: Recognizing and dealing with contamination [Re: TwoOneFiveGold]
    #27237198 - 03/04/21 03:16 PM (3 years, 25 days ago)

You think those loom weird grow some scelortia.


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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OfflineProfessor X
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Re: Recognizing and dealing with contamination [Re: TwoOneFiveGold]
    #27237205 - 03/04/21 03:19 PM (3 years, 25 days ago)

Quote:

TwoOneFiveGold said:
https://https://https://https://Bare with me I'm new to forum's and mushrooms lol but I have a possible problem with my first go at fungus and not sure what's going wrong if anything. Everything went perfect up to fruiting. So trying to keep this short, I inoculated grain spawn with online syringe that took about two weeks but great smell went perfect with no contaminate. The I bulk spawned into coco/perlite/vermiculite substrate into 5 quart about 2-3 inch deep, then covered and let sit 10 day to two weeks. Opened two of them up (did 5 total atm) at 10 days and they looked perfect nothing weird other then what seemed to be a clear film over some parts and seemed a bit wet, after a day or two I noticed some discoloration and tossed both. Cleaned everything top to bottom them opened up then other three at day 15 and 3 days in I'm not sure if its contaminant or bruising after some research and still worried. And comments or help would be extremely appreciated. Thanks all I'm gonna try and put pics up but cam is iffy at best, and dry for long post if I should be somewhere else posting please help me please and thanks 





Hard to tell from those pics? Is that a UV light? They don't need UV. As far as I can tell they look bacterial. Post some better pics. As far as the source, they bacteria probably came from the syringe.


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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OfflineTwoOneFiveGold
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I'm a teapot

Registered: 03/04/21
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Re: Recognizing and dealing with contamination [Re: Professor X]
    #27237272 - 03/04/21 04:06 PM (3 years, 25 days ago)

Thanks for the quick response. They are led lights cool spectrum (almost no uv per company test) but I'm not sure if its my camera or the lights or both cause I thought the same thing. Ill try and get better pics when I check on them soon, I took those pics this morning. Curious what makes it look bacterial? Not sarcastic just looking to educate myself lol. Sadly I didn't label this batch but the next one's are labeled and are from different syringes, so if that's the issue it should be solved. Assuming its not the syringes what would be next on the list? Cause from rye to now I've had no smells or other colors and have been extremely careful. Last question lol if it is bacteria should I toss everything and clean and start over?

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OfflineProfessor X
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Re: Recognizing and dealing with contamination [Re: TwoOneFiveGold]
    #27237305 - 03/04/21 04:26 PM (3 years, 25 days ago)

The mycelium looks thick and matted. That usually happens when there isna bacterial presence.


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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OfflineTwoOneFiveGold
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I'm a teapot

Registered: 03/04/21
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Re: Recognizing and dealing with contamination [Re: Professor X]
    #27237386 - 03/04/21 05:06 PM (3 years, 25 days ago)

I didn't use a casing layer on these cause I hear mixed reviews but I'm gonna try casing the next batch. Here's more pics if it helps. Think I should just toss and clean up? Will they effect everything in the room?https://https://https://https://https://https://

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OfflineTwoOneFiveGold
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Re: Recognizing and dealing with contamination [Re: Professor X]
    #27237400 - 03/04/21 05:15 PM (3 years, 25 days ago)

Also been using h2o2 at about 2% to mist when dry I fan and mist if needed twice a day

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Offlinespitfire2598
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Registered: 03/06/21
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Re: Recognizing and dealing with contamination [Re: TwoOneFiveGold]
    #27240307 - 03/06/21 10:59 AM (3 years, 23 days ago)

I'm doing my first PF Tek Run and im wondering if this is just Mycelium or is this contamination. Would really appreciate some more experienced mycologists taking a look at it!


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OfflineRamJam
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Registered: 10/21/20
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Re: Recognizing and dealing with contamination [Re: spitfire2598]
    #27248618 - 03/11/21 05:15 PM (3 years, 18 days ago)

looks good:thumbup:


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OfflineRamJam
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Re: Recognizing and dealing with contamination [Re: RamJam]
    #27248626 - 03/11/21 05:20 PM (3 years, 18 days ago)

Can you really get worms from the air? I did these BRF jars of ATL 7. Was gonna grab some myc for agar and fruit from the jar, maybe save some for sclerotia idk.  But, I noticed 2 clear contam spots in one jar that weren't colonizing. Said fuck it let's see what happens. Few days later, whole thing is yellow and got worms everywhere.  How the fuck does 1 jar have worms and not the rest if they are all from the same batch noc'ed at the same time?



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InvisiblezMan
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Registered: 09/08/12
Posts: 2,191
Re: Recognizing and dealing with contamination [Re: RamJam]
    #27248643 - 03/11/21 05:35 PM (3 years, 18 days ago)

Quote:

RamJam said:
Can you really get worms from the air? I did these BRF jars of ATL 7. Was gonna grab some myc for agar and fruit from the jar, maybe save some for sclerotia idk.  But, I noticed 2 clear contam spots in one jar that weren't colonizing. Said fuck it let's see what happens. Few days later, whole thing is yellow and got worms everywhere.  How the fuck does 1 jar have worms and not the rest if they are all from the same batch noc'ed at the same time?






My guess is that's from fungus gnats.

Or maybe fruit flies but they like rotting fruit/alcohol.


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InvisibleSmartattack
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Re: Recognizing and dealing with contamination [Re: zMan]
    #27248677 - 03/11/21 05:49 PM (3 years, 18 days ago)

Jesus Christ. We can pen that one into the bad camp.


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OfflineRamJam
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Re: Recognizing and dealing with contamination [Re: zMan]
    #27248832 - 03/11/21 07:19 PM (3 years, 18 days ago)

Holy fuckin shit!! Dude I just got some bananas that I let get brown to make banana bread (which was fucking delicious) and got fruit flies for a few weeks now as a result! fuck me, didn't bother me much but my girl has been flipping on them. now I am too! FUCK THESE FRUIT FLIES!

Edit: just ordered 40 fruit fly traps, 4 different styles, if anyone has fruit fly extermination tek pls post lol


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Edited by RamJam (03/11/21 07:33 PM)

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InvisiblemushboyMDiscord
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Re: Recognizing and dealing with contamination [Re: RamJam]
    #27248847 - 03/11/21 07:39 PM (3 years, 18 days ago)

Quote:

RamJam said:
Can you really get worms from the air?




up until the 17th century people thought maggots spontaneously generated.

talk to starbones about bugs :minigun:

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OfflineRamJam
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Re: Recognizing and dealing with contamination [Re: mushboy]
    #27248882 - 03/11/21 07:57 PM (3 years, 18 days ago)

Quote:

mushboy said:
up until the 17th century people thought maggots spontaneously generated.





I have a feeling many still think that...

Quote:


talk to starbones about bugs :minigun:





cool ty, the gunman is inspirational


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Edited by mushboy (03/11/21 07:59 PM)

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InvisiblemushboyMDiscord
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Re: Recognizing and dealing with contamination [Re: RamJam]
    #27248885 - 03/11/21 07:59 PM (3 years, 18 days ago)

fixed that quote for ya i had too sorry:lol::awesomenod:

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InvisiblezMan
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Re: Recognizing and dealing with contamination [Re: RamJam]
    #27249033 - 03/11/21 09:26 PM (3 years, 18 days ago)

Quote:

RamJam said:
Holy fuckin shit!! Dude I just got some bananas that I let get brown to make banana bread (which was fucking delicious) and got fruit flies for a few weeks now as a result! fuck me, didn't bother me much but my girl has been flipping on them. now I am too! FUCK THESE FRUIT FLIES!

Edit: just ordered 40 fruit fly traps, 4 different styles, if anyone has fruit fly extermination tek pls post lol




if you have some old wine you can use that. You can get super fancy with how you make them but more or less just make sure there's not rotting fruit/veggies/fruits or sweet stuff out and they'll go away on their own.

However I'm not sure that these are actual fruit flies, especially if they've been there for weeks. My guess its they are fungus gnats, they look similar, especially if you don't catch one and looks at it closely. I suggest looking up both online. Also if they are hanging around any of your tubs, its fungus gnats.


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Information for everyone: http://www.mushroomvideos.com
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Edited by zMan (03/11/21 09:29 PM)

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OfflineRamJam
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Re: Recognizing and dealing with contamination [Re: mushboy]
    #27249036 - 03/11/21 09:29 PM (3 years, 18 days ago)

[quote.]mushboy said:
fixed that quote for ya i had too sorry:lol::awesomenod:[/quote.]

ty lol i saw that and was like how'd that happen :lol::awesomenod:

& PMed starbones

Edited by RamJam (03/11/21 09:38 PM)

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OfflineRamJam
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Re: Recognizing and dealing with contamination [Re: zMan]
    #27249040 - 03/11/21 09:31 PM (3 years, 18 days ago)

fuckbuckets, ok, ya, we have left 0 fruit or veggie out since then, and my tubs are as far from kitchen as possible... i'll be lookimg into this further ty for heads up


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InvisiblezMan
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Re: Recognizing and dealing with contamination [Re: RamJam]
    #27249043 - 03/11/21 09:35 PM (3 years, 18 days ago)

No problem. Fungus gnats can be a pain. Last time I had to get rid of all the tubs cause they would get in through the polyfil. The flying gnats aren't the issue other than laying more eggs. The larvae which feed on fungus are the issue.


--------------------
Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST

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