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DJ Ed
Mushroom Engineer


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Agar vs Liquid Culture 1
#26661487 - 05/10/20 02:30 AM (3 years, 9 months ago) |
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Good morning ladies and gentlemen,
I’ve been growing successfully with multi-spore syringes for years now, but recently have started having some success using agar plates to try and grow out an isolated mushroom. But have also started to realise using agar is so much more effort and duration than liquid culture.
Now I am assuming we are not interested in cleaning up samples, rather I am assuming we only want to produce the best crops from already clean stock.......
So I’ve started thinking; isn’t the best way to generate lots of strong (and isolated) mycelium is to clone a vigorous healthy mushroom from your first multi-spore flush into liquid culture? My liquid culture jars are dense within two weeks, after which I can store them in the fridge for months; I have successfully stored LC in the fridge for over a year and it has still been viable.
So what am I missing people? Am I right in thinking there is no benefit to using agar over and above liquid culture?
Thanks DJ Ed
-------------------- “It’s like when you see a mountain lion,” he suggested. “If you run, it will chase you. So you must stand your ground.” Michael Pollan: How To Change Your Mind “The problem is not to find the answer, it’s to face the answer.” Terence McKenna

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ModularMind
M.P.F.



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Re: Agar vs Liquid Culture [Re: DJ Ed] 2
#26661511 - 05/10/20 02:51 AM (3 years, 9 months ago) |
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You cannot see contamination in liquid culture. You either put to agar or risk jars.
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poisoned
untitled



Registered: 04/17/13
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Taking a clone is way more contam prone than making a transfer agar to LC. Cloning directly to LC surely can work, but you're doing that to basically cut a few days from your grow. I don't feel like that's worth it for me.
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mushpunx
Fungus Punk



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Re: Agar vs Liquid Culture [Re: ModularMind] 1
#26661522 - 05/10/20 03:02 AM (3 years, 9 months ago) |
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Liquid culture should *only* be made from a clean culture on agar. It is a prerequisite.
Spore prints are taken from fruits which are grown in open air, they are never fully sterile. This means the spore syringes made from them are never fully clean either. By putting these spores directly into a liquid culture, you will be expanding any mold or bacteria along with it.
The point of agar is to obtain a clean culture. You germinate the spores on agar, and you transfer clean myc away from any bacteria or mold to a new plate and continue doing so until your culture is clean.
When cloning to agar you often have to transfer away from bacteria even though the biopsy was taken from virgin tissue.. it happens. You don't want that in your LC. You clone to agar first, clean up the culture, and use that to inoculate LC .. if you wish to make LC from a clone .
Edited by mushpunx (05/10/20 03:09 AM)
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ModularMind
M.P.F.



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Re: Agar vs Liquid Culture [Re: mushpunx] 1
#26661526 - 05/10/20 03:06 AM (3 years, 9 months ago) |
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Op is talking clone tissue to LC after a successful MS fruiting. Still easily contaminated and only verified clean on agar.
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DJ Ed
Mushroom Engineer


Registered: 09/04/16
Posts: 2,326
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Thank you all for your responses. There does seem to be a general consensus on the contamination, thank you. I have just started a grow with 7 grain jars for a monotub grow (BOD’s Easy AF Unmodified Monotub tek); from a Mazatapec spore syringe, I did a multi-spore grow (1.7kg) and injected a few spores onto agar plates. I grew these out selecting for vigorous growth (never got ANY rhizomorphic growth, just cottony) through 3 generations of agar. I then took a wedge and grew out into a liquid culture. I used this LC to inoculate the rye jars. Wow: the bottom of the jars were colonised in a day 
Thanks all for the feedback; I think these last 7 or 8 weeks in lockdown have turned me really lazy! Was just looking for an easier way
-------------------- “It’s like when you see a mountain lion,” he suggested. “If you run, it will chase you. So you must stand your ground.” Michael Pollan: How To Change Your Mind “The problem is not to find the answer, it’s to face the answer.” Terence McKenna

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poisoned
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Re: Agar vs Liquid Culture [Re: DJ Ed]
#26661714 - 05/10/20 06:22 AM (3 years, 9 months ago) |
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DJ Ed
Mushroom Engineer


Registered: 09/04/16
Posts: 2,326
Loc: UK
Last seen: 2 months, 15 days
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Re: Agar vs Liquid Culture [Re: poisoned]
#26661742 - 05/10/20 06:40 AM (3 years, 9 months ago) |
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Thank you
-------------------- “It’s like when you see a mountain lion,” he suggested. “If you run, it will chase you. So you must stand your ground.” Michael Pollan: How To Change Your Mind “The problem is not to find the answer, it’s to face the answer.” Terence McKenna

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A.k.a
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Re: Agar vs Liquid Culture [Re: DJ Ed] 2
#26661810 - 05/10/20 07:41 AM (3 years, 9 months ago) |
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Technically yeah lc is best for large scale grows but you better have your shit on point.
Also you’d need agar to start the lc initially.
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LAGM2020     
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Markamello
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Re: Agar vs Liquid Culture [Re: mushpunx]
#27239877 - 03/06/21 04:18 AM (2 years, 11 months ago) |
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Mushpunx,
Can you help me with this related question.
I have read in multiple threads that healthy, clean mycelium needs to be isolated on agar and used to make lc.
I have already done this successfully and now have multiple jars of clean lc.
My question is, why shouldn't I use my clean lc to start more lc. I mean, the mycelium is healthy and strong. I dont understand why I shouldn't use 10ml of my clean lc to a new broth using my sterile techniques and have success.
Am I missing something?
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