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OfflineGypsyBastard
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Registered: 11/30/20
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Re: Cultivation General Discussion [Re: The Dalcassian]
    #27236399 - 03/04/21 06:08 AM (2 years, 11 months ago)

@Lenz- nah, the ratio of plates to firewood is so small you really don't smell the plastic.


@tututotutu- seems I owe you an apology actually. I made a comment earlier that I thought I wasn't seeing your posts because you had blocked me and turns  out I had blocked you. Not sure how or recall when but my bad man.

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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,825
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27236459 - 03/04/21 07:27 AM (2 years, 11 months ago)

With clones there’s still a lot of genetics in there. Ime running a clone from tissue is real close to ms.

The tissue samples I’ve grown out have been like spore grows but slightly better and usually with a couple fruits that look like the parent.


You gotta transfer from sectors and run those to find what you’re looking for. In my mind I see it as starting out with 100 strains and then cloning gets you down to  30 but keeps the ones you want in the mix.


So there’s still some digging to do.


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LAGM2020

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Offlineclockworkshroom
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Registered: 02/20/20
Posts: 655
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Re: Cultivation General Discussion [Re: A.k.a]
    #27236462 - 03/04/21 07:29 AM (2 years, 11 months ago)

so the perfect canopy of identical beasts would require sectoring a clone plate usually and growing them out to see which is good?


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p9hu7's clean spawn thread

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OfflineCrackatoa
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Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house Flag
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Re: Cultivation General Discussion [Re: clockworkshroom] * 1
    #27236500 - 03/04/21 08:01 AM (2 years, 11 months ago)

I completely understand what your saying AKA, but I've ran a few clone tissues on agar that don't sector. Would you clone a fruit from a clone grow that has the traits you want to farther narrow the genetics? I haven't had good luck from cloning. I'm trying again year to get me some winners to up my output and be more efficient.


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Crack's Tek's and Shit


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Crackatoa]
    #27236555 - 03/04/21 08:38 AM (2 years, 11 months ago)

Well I guess a lot could depend on how big your tissue sample is too. The PE shoebox in my signature was I think t3 from a clone, and the same culture run off the tissue sample plate was like a spore grow.


I really don’t know any of this for sure, just what I’ve experienced personally.



Yeah lately I have been cloning clones but haven’t fruited any yet so we’ll see. The ufo revert I’ve got about to pin is a clone of a clone of a clone.


I’m working on a making a tamp culture with good caps and just cloned a clone too. Should be on grain soon.


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LAGM2020

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OfflineNootmuskaat
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Registered: 01/13/21
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Re: Cultivation General Discussion [Re: A.k.a] * 1
    #27236619 - 03/04/21 09:22 AM (2 years, 11 months ago)


What 'r thoooooose. Having some dark spots on a few pins.

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InvisibleLenz
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Registered: 08/17/20
Posts: 692
Loc: Fungistan
Re: Cultivation General Discussion [Re: Nootmuskaat] * 1
    #27236774 - 03/04/21 10:51 AM (2 years, 11 months ago)

Ahh that’s really interesting, I did start to see a lot of sectoring on my clone plates recently but it was around T3/T4.

In that case though how the hell do you even test all the potential sectors that a tissue transfer plate might put out? Do you have to keep transferring all the sectors until you don’t see any more sectors and then test? I’m just not sure how that works because what happens when you transfer a sector and then it continues to sector and how do you keep track of the all of it? I’m probably overthinking but how do most people treat clones to get a banging culture they can run for years?

When I first started cloning I did have some plates that didn’t sector at all after like T3 but I haven’t seen that since:


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:chems:

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Offlinetututotutut
You...! You're not me!
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Registered: 01/18/21
Posts: 2,218
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Re: Cultivation General Discussion [Re: Lenz] * 1
    #27236840 - 03/04/21 11:29 AM (2 years, 11 months ago)

Great work lenz!! Keep it up!!
@Gypsy lol please, don't worry about it. I owe the entire community right now.


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yeah keep telling yourself that's voluntary

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Offlinesahle
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Registered: 03/04/21
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Re: Cultivation General Discussion [Re: Inocuole] * 1
    #27236843 - 03/04/21 11:30 AM (2 years, 11 months ago)

So... I've moved into a new place and its pretty cramped and not ideal for growing anything. Its also cold as hell here, I'd prefer not to use space heaters or radiators or TiT methods or heat bombs. :tongue:

However! There is a small boiler room/space that houses a hot water cylinder tank, its a sweet 75-77f in there at all times which is perfect, and also has a convenient little shelf. I've thrown up a quick sketch and I'm just wondering... would this work...? The door would be shut of course.

The only problems I can think of are FAE. Its pretty much sealed once the door is shut, the only sort of breathing space for this place is a small plastic vent in the door. The vent gaps are small though, I doubt the space gets much air. Also its dark, dark as hell. No light gets into this thing. Also the humidity is 40% which might be a problem.



Now I know I know, KISS right? I've never had a problem with FAE or air circulation before so this is my first time working with filters and fans and the sort. I was thinking of leaving the little fan on a low speed at all times? So it intakes air. If I could get any input on this set-up at all I'd really appreciate it :tongue2:

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Offlinetututotutut
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Registered: 01/18/21
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Re: Cultivation General Discussion [Re: sahle]
    #27236847 - 03/04/21 11:31 AM (2 years, 11 months ago)

sahle have you read about growing truffles? jars just set it and forget it no misting no fae stuff


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yeah keep telling yourself that's voluntary

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Offlinesahle
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Re: Cultivation General Discussion [Re: tututotutut]
    #27236870 - 03/04/21 11:47 AM (2 years, 11 months ago)

I've never looked into it but that does sound interesting and convenient... might have a few of those on the side :laugh:
For this grow i'm just thinking about ordinary cubes

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Offlinetututotutut
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Re: Cultivation General Discussion [Re: sahle]
    #27236885 - 03/04/21 11:58 AM (2 years, 11 months ago)

Pfffftt cubes shmubes! Truffles are really cool! Lots of resources here on the site I think there's a truffle discussion too!

https://www.shroomery.org/forums/showflat.php/Number/17910649

Much like cubes, workflow possibilities are endless. You could even do the Pf tek, take spore prints to get x more spores from the initial syringe, maybe even clone the fruits or the cakes back to grains and you already got your first round of truffles going! They have to sit longer in fruiting than traditional mushrooms but again, zero maintenance just stash some jars some where :smile:


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yeah keep telling yourself that's voluntary

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Invisiblealaskappalachian
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Registered: 10/22/19
Posts: 1,702
Loc: The 49th Dimension
Re: Cultivation General Discussion [Re: sahle] * 2
    #27236890 - 03/04/21 11:59 AM (2 years, 11 months ago)

It'll get air without a fan.  Just throw up a LED strip or fluorescent and your'e good to go. :thumbup:

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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,633
Re: Cultivation General Discussion [Re: The Dalcassian] * 1
    #27236980 - 03/04/21 01:00 PM (2 years, 11 months ago)

Quote:

The Dalcassian said:
So this is the last tub I'm doing before I move house and it decides to be a funny fucker. My lagm grow too... typical.



Anyway, as you can see, its got this thick creamy ass growth on top. I knocked some condensation of the lid onto it and it bruised blue, good sign I suppose, and it smells good.

So its probably bacterial, just wondering if scraping a bit of that myc of the top might help get a fruit or two out of it, I have a T1 in the fridge I can work with but it'd be nice to have something to show for lagm before my hiatus





Hey Dalcassian
That looks a lot like one of my first PE tubs. I am not sure what was off about it, but it did eventually produce a decent few flushes. The myc was exhausted before it ever contamed out. So I would hold out hope for it.


I dont' have a picture of the later flushes in the tub. That is the first flush from mss.


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LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic



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OfflineThe Dalcassian
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Re: Cultivation General Discussion [Re: ReverendMyc] * 1
    #27236983 - 03/04/21 01:03 PM (2 years, 11 months ago)

Thats put my mind at ease a bit, your growth there is almost identical to what I've got atm. I'll hang tight with it


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Here Lies This Individual




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Offlinetututotutut
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Re: Cultivation General Discussion [Re: The Dalcassian]
    #27236987 - 03/04/21 01:05 PM (2 years, 11 months ago)

Don't forget you can still take spores if it gets contaminated but still fruits :smile: Might be a little extra work on the agar, but I think that's better than nothing


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yeah keep telling yourself that's voluntary

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InvisibleWall.E
Bacteria's Bitch
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Registered: 06/05/20
Posts: 2,860
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Re: Cultivation General Discussion [Re: The Dalcassian] * 1
    #27236991 - 03/04/21 01:07 PM (2 years, 11 months ago)

I had a tray that looked like this

I broke it up into more coir and got these little retarded fruits from it



I wouldn't say it's the same thing as yours though, mine was fucked


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Life’s shit, but I’m loving it


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OfflineNootmuskaat
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Registered: 01/13/21
Posts: 42
Last seen: 2 years, 10 months
Re: Cultivation General Discussion [Re: The Dalcassian]
    #27237008 - 03/04/21 01:16 PM (2 years, 11 months ago)

Quote:

The Dalcassian said:
So this is the last tub I'm doing before I move house and it decides to be a funny fucker. My lagm grow too... typical.



Anyway, as you can see, its got this thick creamy ass growth on top. I knocked some condensation of the lid onto it and it bruised blue, good sign I suppose, and it smells good.

So its probably bacterial, just wondering if scraping a bit of that myc of the top might help get a fruit or two out of it, I have a T1 in the fridge I can work with but it'd be nice to have something to show for lagm before my hiatus



Interesting. One of my shoeboxes is exactly the same. B+ MSS agar to grains.


Just like yours it smells great. But it doesn't look like it's interested in forming primordia anytime soon. While my Mckennaii clones are pinning, this one is chomping up the top layer.

Edited by Nootmuskaat (03/04/21 01:17 PM)

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Offlinesmalltalk_canceled
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Registered: 07/13/20
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Re: Cultivation General Discussion [Re: Nootmuskaat]
    #27237018 - 03/04/21 01:23 PM (2 years, 11 months ago)

Anyone else thinking bacteria?


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Willpower is the one true virtue


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OfflineNootmuskaat
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Registered: 01/13/21
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27237025 - 03/04/21 01:27 PM (2 years, 11 months ago)

Quote:

smalltalk_canceled said:
Anyone else thinking bacteria?



Could be. All my jars were slightly bacterial, but my 3 other shoeboxes are doing great. Those were clones though, this was from a MSS.

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