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 Jason400
Stranger
Registered: 07/08/20
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Last seen: 1 day, 17 hours
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 Best way to start selections for cloning.   1
#27202322 - 02/12/21 08:40 PM (13 days, 17 hours ago) |
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I am new at mushroom cultivation but my background is in the Bacterial, Cell, and Virus production in the vaccine industry.
I just started some plates using PC B+ a few days ago. My plan was to transfer the polyclonal mix to grain spawn then to tubs. From there I would choose the fruits with the best phenotypes and start cloning from there. Or should I start with choosing areas from the plate and transferring that to new plates before inoculating grain?
Are there any draw backs to these procedures. Any advise is welcome.
Thanks in advance
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Hindsight
Mad Scientist
Registered: 01/24/21
Posts: 49
Last seen: 15 hours, 15 minutes
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Re: Best way to start selections for cloning. [Re: Jason400]
#27202405 - 02/12/21 09:17 PM (13 days, 16 hours ago) |
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Hoping someone with more experience replies but I've been researching this quite a bit lately and it seems there are two schools of thought (one for each of the options you laid out).
The issue with isolating is that you have no idea what you are isolating... no idea if the particular mycelium you isolated is any good. It could be great or it could be awful. Whereas if you just dump your plate that is growing out multiple mycelium breedings, all those will grow in your tub and you'll get to see the results of all of them. You then select the best fruits and clone and/or print them from there (depending on what you are trying to do). For something that is already really domesticated (like B+), you likely won't have that much genetic diversity so going with clones from that point forward is probably your best bet (as opposed to repeated breedings from spore).
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 Crackatoa
Stranger in a strange land
Registered: 04/01/19
Posts: 3,480
Loc: Over by your Mama's house 
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Re: Best way to start selections for cloning. [Re: Hindsight] 2
#27202592 - 02/12/21 10:34 PM (13 days, 15 hours ago) |
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You wanna pick the fastest strongest myc from your petri dishes till you get a clean culture and put that to grain. That being your master jar. You can expand G2G if you wanna go to a larger tub for a bigger selection or use the one to a shoebox. Once you get fruits, THEN you clone. I always go for a tissue sample of the first cluster to pop up and I get a sample from a fruit of choice, Biggest, prettiest. Starting from a multispore, taking tranfers you are not reducing genetics by enough to take out "promising" ones.
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MikeRodose
Stranger
Registered: 02/23/21
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Re: Best way to start selections for cloning. [Re: Crackatoa]
#27221516 - 02/23/21 06:21 AM (3 days, 7 hours ago) |
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Crackatoa - you just made my day!
I was looking for this advice and was taking a quick look before posting a question - I was stressing that I would "miss out" on something by going from MSS to Agar and selecting transfers
quote]Crackatoa said:
You wanna pick the fastest strongest myc from your petri dishes till you get a clean culture and put that to grain. That being your master jar. You can expand G2G if you wanna go to a larger tub for a bigger selection or use the one to a shoebox. Once you get fruits, THEN you clone. I always go for a tissue sample of the first cluster to pop up and I get a sample from a fruit of choice, Biggest, prettiest. Starting from a multispore, taking tranfers you are not reducing genetics by enough to take out "promising" ones.
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 bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 60,309
Loc: Milky way
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 Re: Best way to start selections for cloning. (moved) [Re: Jason400]
#27221531 - 02/23/21 06:47 AM (3 days, 7 hours ago) |
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 This thread was moved from Advanced Mycology.
Reason:
Regular part of cultivation
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