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Offlineriomar
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How fucked up are these jars grains?
    #27216743 - 02/20/21 01:26 PM (3 years, 10 months ago)

Hey guys...
Need some reality check here.
I see grain prep was not good. A lot of extra moisture.
I dunt see contamination, but the growth has being very slow lately.
3 different boxes (pp5) with a hole and stuffed polyfil.
They were inoculated from agar 22/1.
Are they done and should I feed them to the compost?
Temperature has been in a variable range between 70 to a max of 85.
Most of the time around 80.

Thanks to all answers


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InvisibleSmartattack
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Re: How fucked up are these jars grains? [Re: riomar] * 1
    #27216755 - 02/20/21 01:32 PM (3 years, 10 months ago)

Those go from bad to terrible unfortunately. Check out the official spawn thread that's going.

https://www.shroomery.org/forums/showflat.php/Number/27187543


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OfflineLand TroutM
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Re: How fucked up are these jars grains? [Re: Smartattack]
    #27216764 - 02/20/21 01:36 PM (3 years, 10 months ago)

I would expect those to go to mold pretty quick if you spawned them.  Looks like good food for the compost bin.

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OfflineLogicaL ChaosM
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Re: How fucked up are these jars grains? [Re: riomar]
    #27216887 - 02/20/21 02:49 PM (3 years, 10 months ago)

Looks like a bad bacteria problem from excessive moisture. Its not good :nonono: Im afraid you gotta compost it.


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OfflineJosborne
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Re: How fucked up are these jars grains? [Re: riomar]
    #27217350 - 02/20/21 07:47 PM (3 years, 10 months ago)

Quote:

riomar said:

I see grain prep was not good. A lot of extra moisture.
I dunt see contamination, but the growth has being very slow lately.





Looks like bacteria. Growth has stalled.

Quote:


Are they done and should I feed them to the compost?





The first one (or which ever is the most colonized) might actually be viable for spawning to bulk substrate. Mold contamination should always go straight to the trash; bacterially contaminated jars on the other hand can be spawned to bulk, with varying results (might stall, but you'll probably get a decent flush or two). Don't make a habit of it but if you have the extra substrate and tub then why not


Quote:


Temperature has been in a variable range between 70 to a max of 85.
Most of the time around 80.





80 is the upper limit. 85 and you're just asking for trouble. If you can, keep it around 70-75.

Edited by Josborne (02/20/21 07:48 PM)

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InvisiblezMan
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Re: How fucked up are these jars grains? [Re: Josborne]
    #27217432 - 02/20/21 08:51 PM (3 years, 10 months ago)

Bad. How long did you PC? Did you simmer and let them dry? If its not the PC time then the agar is bad.


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Are your grains or spawn clean? CLICK THIS CHECKLIST

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Offlineriomar
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Re: How fucked up are these jars grains? [Re: zMan]
    #27217761 - 02/21/21 02:41 AM (3 years, 10 months ago)

Guys, thanks for the answers.
I see the humidity is very bad, I certainly failed with the grain.
It wasn't dry enough, PC might also have not been enough.
For what I see and what I read, there might be multiple factors but seems like the humidity is the main trigger.
I prepared some more grain recently i think I got it better, I also went full 90 minutes on the pc, I was a bit careful with the pc going dry y cut between 60 to 90.

The new ones look better.

I'm also succeeding with a mono from the previous preparation so I'm cool with all this... The process of learning.

So my question now is, i toss away The two that look worst. And I can try to deal with the one that is almost fully colonized.
1. Should I remove the uncolonized grains? Like kind of wash it a bit?
2. I read @josborne advice to spawn it... Resources around here are not so easy/cheap... Should I be more to the safe side by treat it as a cake? And try to fruit it like that?
3. How would I take care of it? Like remove visible contamination when i see it? Or something else?

I read in the thread shared by @smartattack that contaminated spawn can be used but I'm not sure about the treatment, I'll check b on the searcher to find something about it. Advice is welcome.

Guys this is really helpful I'm very grateful...

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InvisiblezMan
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Re: How fucked up are these jars grains? [Re: riomar]
    #27218091 - 02/21/21 09:06 AM (3 years, 10 months ago)

If resources are scarce I wouldn't spend anymore on those jars.

If you really want to you can try spawning it all to a shoebox and see what happens, just rid of the uncolonized parts. BUUUTT seeing as you're new and likely wouldn't recognize trich until it was green, I would avoid doing it and possibly spreading billions of spores into your house.


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Are your grains or spawn clean? CLICK THIS CHECKLIST

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Offlineriomar
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Re: How fucked up are these jars grains? [Re: zMan]
    #27244838 - 03/09/21 11:04 AM (3 years, 10 months ago)

Hey guys!
Checking in with you again...
I did spawn it to a small mono. I didn't have much substrate but anyway, i didn't have much grains and I might have to interrupt the growing soon so... I gave it a go...

Now the small tub is colonized and pins are showing up. But I'm wondering if I'm also seeing some molds on the surface, or of it's the mycelium still growing. The strain is purple mystic, it's much slower than the golden teacher that i grew in parallel. And less successful...

So guys, could you take a look at this picture? And give me some feedback?
1. Green is a pin, and there's a nice overall pinning.
2. Blue is mycelium, everything ok there.
3. Red, what do you say? Is mycelium in the process of colonizing, or is a mold? And if it's a mold, how can I deal keep up with it too harvest something?


I read the answers and other posts, and I know in some sense i took the wrong decision, but I also want to see this unfolding, as zMan said, I don't think I can recognize those thrice until they come green 🤣 I would like to learn to 👍


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InvisibleJosex
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Re: How fucked up are these jars grains? [Re: riomar]
    #27244855 - 03/09/21 11:13 AM (3 years, 10 months ago)

1. Yes it's a pin, not the only one in the pic though.
2. No problems there, hyphal knots and stuff.
3. Looks like cube myc to me.

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OfflineLand TroutM
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Re: How fucked up are these jars grains? [Re: riomar]
    #27244858 - 03/09/21 11:14 AM (3 years, 10 months ago)

Cool.  I can’t tell if that’s mold or cube myc.  I’m actually glad you spawned those and kept up the post.  Those grains looked so bad, I am suprised they made it this far.  But looks like you might get enough fruits to make it worth while.
I would say the biggest thing to look for is bright white mycelium and that would be trich, the cubensis is white also, but early trich before it spores is brilliant white.

Edited by Land Trout (03/09/21 11:16 AM)

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Offlineriomar
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Re: How fucked up are these jars grains? [Re: Josex]
    #27245076 - 03/09/21 01:56 PM (3 years, 10 months ago)

Quote:

Josex said:
1. Yes it's a pin, not the only one in the pic though.
2. No problems there, hyphal knots and stuff.
3. Looks like cube myc to me.




Hey Josef! Are you spanish speaking?
You're right, not the only one, I'm seeing que a nice pinning and I'm hair about it!
I hope it is my right away, I'm guessing if it would be contam i would have seen more issues... I had to put out 2 of this boxes with this strain... More than anything I'm thinking it's about the grain prep and the sterilization.
Thanks!

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Offlineriomar
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Re: How fucked up are these jars grains? [Re: Land Trout]
    #27245088 - 03/09/21 02:02 PM (3 years, 10 months ago)

Quote:

Land Trout said:
Cool.  I can’t tell if that’s mold or cube myc.  I’m actually glad you spawned those and kept up the post.  Those grains looked so bad, I am suprised they made it this far.  But looks like you might get enough fruits to make it worth while.
I would say the biggest thing to look for is bright white mycelium and that would be trich, the cubensis is white also, but early trich before it spores is brilliant white.




Well, many thanks for the support!
I'm not seeing bright white..
I'm just seeing in what i pointed in the picture the my is not as dense, is more like how a bread mold would look like... kind oh more foamy. But that's what I'm unsure about, because the colonizing seems to be like that before it colonizes and gets more dense and solid like. Right? Is just that the consolidated myc seems easy for me to identify.. but this "in the process" gives me shivers🤣
I'm glad i did it as well, i guess i will be able to take a print and at least have some fruits.
I'll be happy to update.
Thx!

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Offlinezakzwijn
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Re: How fucked up are these jars grains? [Re: riomar]
    #27245248 - 03/09/21 03:21 PM (3 years, 10 months ago)

These are definitely too wet, too much moisture facilitates bacteria contamination as others have pointed out. Have you checked out this site:

https://www.mushroomvideos.com/

it contains useful instruction video's about substrate preparation, how to prevent excess moisture and much more.

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InvisiblezMan
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Re: How fucked up are these jars grains? [Re: zakzwijn]
    #27245475 - 03/09/21 06:15 PM (3 years, 10 months ago)

So far so good. Could be your grains were too wet and it colonized weird.

I wonder if you get more than 1 flush out of it.


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Are your grains or spawn clean? CLICK THIS CHECKLIST

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OfflineProfessor X
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Re: How fucked up are these jars grains? [Re: zakzwijn]
    #27245690 - 03/09/21 08:59 PM (3 years, 10 months ago)

Quote:

zakzwijn said:
These are definitely too wet, too much moisture facilitates bacteria contamination as others have pointed out. Have you checked out this site:

https://www.mushroomvideos.com/

it contains useful instruction video's about substrate preparation, how to prevent excess moisture and much more.



You can find every last bit of the information on that site and more right here. No need to look elsewhere.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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InvisiblezMan
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Re: How fucked up are these jars grains? [Re: Professor X]
    #27245776 - 03/09/21 09:51 PM (3 years, 10 months ago)

Quote:

Professor X said:
Quote:

zakzwijn said:
These are definitely too wet, too much moisture facilitates bacteria contamination as others have pointed out. Have you checked out this site:

https://www.mushroomvideos.com/

it contains useful instruction video's about substrate preparation, how to prevent excess moisture and much more.



You can find every last bit of the information on that site and more right here. No need to look elsewhere.




mushroomvideos is like $8 and made by RR who has contributed LOADS of information here, so its WELLLLLL worth it.

If any other legends offered something similar with videos and such that was easy for newbies to comprehend, I would gladly pay for it and tell them to do the same, just my 2 cents.


--------------------
Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST

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Offlinehongito33
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Re: How fucked up are these jars grains? [Re: riomar]
    #27245898 - 03/09/21 11:50 PM (3 years, 10 months ago)

Riomar,

Those grain are contaminated with bacteria likely caused by the high temps the grains reached while colonizing.Those grains are done for. you would be better of starting all over and make sure to keep your ambient temperature at 70 to 73F while monitor your grains temperature and keep between 76-80F.

Good luck

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InvisiblezMan
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Re: How fucked up are these jars grains? [Re: hongito33]
    #27246094 - 03/10/21 05:09 AM (3 years, 10 months ago)

Quote:

hongito33 said:
Riomar,

Those grain are contaminated with bacteria likely caused by the high temps the grains reached while colonizing.Those grains are done for. you would be better of starting all over and make sure to keep your ambient temperature at 70 to 73F while monitor your grains temperature and keep between 76-80F.

Good luck




Must've not really the part of the thread where he spawned them and the tub is fruiting eh?


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Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST

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OfflineProfessor X
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Re: How fucked up are these jars grains? [Re: zMan]
    #27246225 - 03/10/21 07:48 AM (3 years, 10 months ago)

Quote:

zMan said:
Quote:

Professor X said:
Quote:

zakzwijn said:
These are definitely too wet, too much moisture facilitates bacteria contamination as others have pointed out. Have you checked out this site:

https://www.mushroomvideos.com/

it contains useful instruction video's about substrate preparation, how to prevent excess moisture and much more.



You can find every last bit of the information on that site and more right here. No need to look elsewhere.




mushroomvideos is like $8 and made by RR who has contributed LOADS of information here, so its WELLLLLL worth it.

If any other legends offered something similar with videos and such that was easy for newbies to comprehend, I would gladly pay for it and tell them to do the same, just my 2 cents.


I watched those old videos years ago. He does some wrong things on there and teaches a few bad habits. It's mostly good stuff though.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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