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Offlinehazyhorse
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contamination records * 1
    #27210398 - 02/17/21 02:42 AM (2 years, 11 months ago)

just making this journal to keep track of my spawn & what was/wasn't contaminated. i want to get better at spotting it, as i miss a lot of the non-obvious stuff. i thought it would be nice to have side by side pictures of the jars & the tubs they produced


PE6 clone:

these jars look sketchy (spiky myc, a few to many thick & milky points, those grains pressed against the glass etc) however the one jar i shook to test seems to have recovered completely to at least the same degree as the other jars, as i can't even tell which one i shook. broke apart pretty easily; didn't have to kill my hands breaking these grains. not expecting them to do well by any means, just spawning them for my own reference.



i should add that these jars were inoculated g2g with a 1/2 master jar i've had in the fridge since probably... like august or september? almost 8 months & clearly the cold nap didn't slow them down at all! i was planning on storing master cultures on grains & seeing how that goes, as i read an old post by RR mentioning storing on grains long term was a good way to keep cultures, but that jars generally took up too much room in the fridge for it to be viable. i feel like using 1/4 pint jars would be small enough to make it viable. if i could keep grains in there long term, i could take one grain out of the jar to inoculate a plate & then keep that like verum subsequentis talks about in this thread. i could get away with not using slants. i want to learn how to use them eventually but right now i'm really drawn to the idea of storing on grains for some reason. it just seems so much easier



update (3/4):

tub is finally starting to pin! seemed a little slow to colonize, though i forget if this clone was fast or not the last time i grew it out. it didn't seem to fully colonize the casing i put on at spawn, which happens sometimes even with healthy spawn i've had, but i'm assuming the potential bacteria wasn't helping anything. looks like it should pin solidly too!





update (3/10):

harvested some of the first fruits of this tub! forgot to take a before pic but there were a lot more shrooms than it looks like there were. the ones i harvested were some larger clusters & caps. leaving the rest to mature a bit, as there were a lot of smaller pins & i didn't wanna disturb the sub too much yet. it's side pinning like a motherfucker despite the liner, i think i'm gonna play around with tamping down my subs rather firmly in the future. another member said they did that with their clone that only ever wanted to side pin & saw results





update (3/12):

total dry grams for the first flush was 34.1g. not too shabby for 2 quarts of bacterial looking spawn. waiting on the second flush now, if it doesn’t contam first.



update(3/15):

second flush is coming in super solid!! really stoked on the pin set. all i did was give it a two or three solid mists over the past few days





update (3/17):

looks like trich is starting to break out. will upload pics when i'm a supporter. gonna let the flush ride since it looks like the fruits are about half way done now.



(got my supporter status (: )



update (3/19):


(i keep forgetting to take pics before i start harvesting LOL)

just dried the second flush, came out to 12.4g. total harvest between both flushes was 1.64oz for 2 quarts spawn. not the best return obviously but still more than decent. most of the trich is on the sides rn so i’m gonna see if i can squeeze a third flush out of it, i’ll probably end up tossing it before then though


update (3/23):

trich is breaking out on the surface, gonna toss the tub. she gave a solid 2 flushes, i can't really complain at all.


Edited by hazyhorse (06/06/23 07:42 PM)


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Offlinehazyhorse
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Re: contamination records [Re: hazyhorse] * 1
    #27221355 - 02/23/21 12:35 AM (2 years, 10 months ago)

mysteryberry:

spawned these random white clones i got from a normal cube monotub. they drop purple spores but are completely white it’s weird. it was mentioned that this spawn looked kinda bacterial, but i’m growing these out for my own records so i’m expecting them to do kinda poorly. spawned 1.5qt of oats to a shoebox. hoping the higher spawn ratio helps it produce OK. broke up easily, did not smell particularly off or anything. the inconsistency of the myc & some of the wet spots are why i’ve been told it’s bacterial. they were inoculated with agar wedges.

unshaken (had a tiny spot on top that wasn’t covered in myc but i didn’t have time to give it an extra day. i was gonna just pull that grain out but i forgot. oops)



the two on the left were shaken a few days before this pic myc looks like its recovered weakly & there are a lot of wet spots on the glass (the bottom right jar has weird glare on it but it was all white)


update (3/4):

this tub colonized fast! this clone is on the more aggressive side compared to some of my other cultures, but i think the extra pint of spawn helped things go a little faster too. looks like it's about to start pinning real soon



update (3/10):

got a pretty solid pinset coming in on this tub!! this clone fruits pretty nicely already but i think the addition of the liner kept things perfect in this case. i'm shocked; i was expecting this tub to do way worse. the mycelium was getting weird & thick & almost blobby in patches (i'll have to upload an image i took of this from a few days prior) so i thought for sure it was gonna go south soon, but it seems to be doing fine. there's still time for things to go wrong of course, but i think i'll get at least one flush from this one. i think the weird mycelium was due to bacteria that i'm assuming was in these jars



update (3/11):

i keep forgetting to take pics before i start harvesting lol. rlly happy w this tub, the clone has always been a solid producer for me



came out to 20.5g dry


update (3/15):

the pin set is looking decent for this second flush!





update (3/17):

small spot of trich on a mushroom stump. gonna let the flush ride & then trash the sub



update (3/19):



second flush put out 12g, totaling 1.16oz from 1.5qt of spawn. not too shabby. the potency on this clone is pretty good too so no complaints from me! the surface of the sub was starting to noticeably trich out so i just tossed it


Edited by hazyhorse (03/23/21 04:07 PM)


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Offlinehazyhorse
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Re: contamination records [Re: hazyhorse]
    #27238178 - 03/05/21 06:05 AM (2 years, 10 months ago)

golden halo clone (non-golden spore):

these jars look bacterial to me. as with the other jars, these ones have heavy condensation, wet spots & grain pressed against the glass. they weren't super hard to break up but were a bit more resistant than other jars i've had. first jar is unshaken, second jar was shaken a few days ago & seems to have recovered weakly. they didn't smell bad or anything, & it's kinda hard to smell things for me sometimes bc i have a bad sense of smell & i had smoked weed right before this (which always kills my sense of smell), but they smelled off. it wasn't bad or sweet or anything, they just didn't have that earthy scent mushroom mycelium usually has. spawned 4 pints (2 quarts) to a sweaterbox. i used a bit less coir bc i saw a post from AKA recommending to use less coir to let the myc colonize faster & since i'm about 99% certain these jars are bacterial i decided to give it a go. inoculated with agar wedges.




update (3/15):

lookin sus lol



it's kinda like that weird matting that the mysteryberry had but over way more of the surface. maybe i'll get a good pin set out of it?



update (3/20):

actually looking like a pretty solid pinset!






update (3/21):

pins are coming in solidly! not my best pin set but hey i'll take it for how gnarly shit was looking






update (3/23):



harvest time! (look, i remembered to take a picture before i started!!) drying the first flush now.

totally forgot to update this when i weighed the flush. i wanna say i only got like 12-14g dry off of this tub



update (3/30):



tossed after the second flush finished as trich set in


Edited by hazyhorse (05/05/21 05:35 PM)


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Offlinehazyhorse
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Re: contamination records [Re: hazyhorse]
    #27256613 - 03/17/21 02:54 AM (2 years, 10 months ago)

golden halo (spores):

these jars def look bacterial. weak myc, lots of condensation. will upload pics when i get my supporter status, but spawned these today. they are from a golden print, so hoping to get gold spores if i get mushrooms. these look bacterial to me. spawned 2qt to a shoebox just to get quick colonization & see if a high volume of spawn to a smaller volume of coir will give me a fast flush.




update (4/28):

shroomery was down during this time, only really got pics of the first flush. spores weren't golden, unfortunately /: but here was the result of the jars. had some absolutely beautiful spore swirling on the caps



contam'd during the second flush so i tossed it after the second flush finished


Edited by hazyhorse (04/28/21 03:03 AM)


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Offlinehazyhorse
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Re: contamination records [Re: hazyhorse]
    #27256619 - 03/17/21 03:00 AM (2 years, 10 months ago)

golden halo clone (non-golden spore):

same clone as the one a post above. spawned 2.5qt to a 27qt mono. pressed the fuck out of it & packed the coir top layer down firmly. these jars look bacterial, hoping the extra half quart & the smaller volume of coir helps it fruit before contams set in





update (3/25):

tub is fully colonized!! getting some yellowing on top, looks like metabolites. surface may be a little too moist, but since this spawn looked so bacterial i’m not surprised. going out of town but will be curious to see how the pinset comes in





update (4/28):

this tub did decently. forgot to take pics before harvest again since the shroomery was down, went out of town & came back to the spore explosion of a second flush you see in the pics lol. didn't get the final weight but performed decently well for being bacterial, i wanna say around 40g or so



got trich after the second flush so i tossed it


Edited by hazyhorse (04/28/21 03:04 AM)


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Offlinehazyhorse
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Re: contamination records [Re: hazyhorse] * 1
    #27295878 - 05/05/21 05:34 PM (2 years, 8 months ago)

golden halo clone (non-golden spore):

jars are definitely bacterial, didn't recover from a shake well at all. you can see the exposed grains & condensation, along with the weird stressed myc. i threw a few of these jars out (the one in the pic of the bottom of the jar i def tossed- it was looking way too gnarly). i think i had 6 or 7 of these jars & spawned 4 of them? i forgot how many i put to these shoeboxes



tub is colonized bc i was lazy in updating this thread, but there is some weird blobbing mycelium going on in two spots





update (5/10):

this pinset looks fucking amazing! honestly might end up being one of my best. i’m not using a liner in this tub & just pressed the fuck out of it at spawn instead. seems to have worked (: i still have some side pinning but holy god there are a lot on the surface. excited to see how this canopy comes in




update (5/12):

had a really good flush off this sub! didn’t use a liner & just pressed the ever living fuck out of it when i spawned & got minimal side pinning compared to what i usually get. still had a little on the sides & bottom but damn the top was so covered in pins i think it just HAD to fruit elsewhere. unfortunately a lot of those little pins didn’t come in with the first flush, but i’m excited to see flush 2 come in





update (5/18):

some stumps are starting to trich out. gonna let the second flush ride & then toss. may put some salt on the trich spots just to potentially stop some  sporulating?




update (5/21):

pulled 24.5g between the first two flushes. probably coulda squeezed out a bit more but didn’t wanna let the trich go much longer


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Edited by hazyhorse (05/22/21 03:47 AM)


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Offlinehazyhorse
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Re: contamination records [Re: hazyhorse]
    #27296656 - 05/06/21 07:40 AM (2 years, 8 months ago)

rustywhyte spores:

these jars were looking decent until they consolidated a bit more. the initial growth was really promising, & the jars i shook recovered pretty well. but towards the very end of colonization there were some spots where the myc thickened up, & i was slow to spawn them so they had extra time to consolidate. it’s also been a bit hotter in my room, so i wonder if that exposed the bacteria more. spawn is looking bacterial to me now with that creaminess & grains pressed against the glass




update (5/30):

oops, got lazy lol. harvested these about two days ago, here are pics of the tubs from a few days before harvest & then on harvest day. i think i mixed the plates/jars up LOL. i got a few white mushies but they were mostly normal looking. oh well. flush came in densely but i think my sub was too dry for the shrooms to really bulk up. will be dehydrating them soon



update (6/7):

harvested 2.95 between the two tubs! i think i used 4 quarts all together & didn’t bother harvesting side or bottom mushies on either flush so i’m pretty happy with it. will upload second flush pics later

update (way later):

realized i never finished updating this tub. p sure it trich'd out


Edited by hazyhorse (02/24/22 08:19 PM)


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Offlinehazyhorse
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oyster mushrooms [Re: hazyhorse]
    #27329601 - 05/30/21 08:03 PM (2 years, 7 months ago)

oysters:

trying my hand at gourmets! these are definitely bacterial, but i'm excited to get some fruits off of them. i'm growing phoenix & pink oysters.



update (8/26):

lol these didn't really go anywhere. i think i need a martha chamber, or at least need to keep them in bags longer before giving FAE


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Edited by hazyhorse (08/26/21 01:28 PM)


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Offlinehazyhorse
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??? [Re: hazyhorse]
    #27329603 - 05/30/21 08:06 PM (2 years, 7 months ago)

mysteryberry (clone):

spawned 3qt to a 30qt(ish) monotub. these jars looked decent to me, but the slight wet spots & condensation has me assuming they are bacterial. this culture is really aggressive, & looked like it was starting to pin in the jars because i had left them on the shelf for awhile. i should have shaken one to see recovery, but oh well.



update (6/21):

i fucked up & only got a horrible pic of the tub, but it had a huge patch of trich. it didn’t colonize at all &then started to trich out after about 20 or so days. i had it on the floor & my room got hella cold, like 59 degrees so i’m assuming it was just really slowed down by the floor temps. this is the first tub i’ve lost funny enough, but oh well. can’t win em all. i have a feeling this woulda at least gotten a first flush in warmer conditions but alas


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Edited by hazyhorse (06/24/21 11:48 PM)


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OfflineAspiringmycologist
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Re: ??? [Re: hazyhorse]
    #27341716 - 06/09/21 02:14 PM (2 years, 7 months ago)

This has been an interesting read. How many flushes would you say you average with a healthy spawn? Does trich set in eventually on those tubs as well? Awesome stuff! Did you notice any difference in the length of time from s2b to pinning from one of your test tubs vs healthy tubs? I have an unsupported hypothesis that contaminated tubs would fruit faster when the mycelium (recognizes) it’s imminent demise but again this is unsupported, untested, and I’m just a noob haha


Edited by Aspiringmycologist (06/09/21 02:22 PM)


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Offlinehazyhorse
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Re: ??? [Re: Aspiringmycologist]
    #27341814 - 06/09/21 03:27 PM (2 years, 7 months ago)

i’m glad you found it interesting! i wanted a resource like this just for my own edification & figures others might find it helpful too since bacterial spawn is so common

with my healthy subs in the past, i usually didn’t find it worth going past two. sometimes i was lazy & would leave it for a third flush, but you definitely get diminishing returns after the second. sometimes third flushes were neat with a few really large fruits, but idk that it would be worth it to go for four unless you didn’t have any other grain waiting to be spawned. maybe trich would have set in had i left them for longer, but in my experience, if spawn is unclean trich will set in between the first & second flush depending. from my understanding, if your spawn is truly clean it won’t ever contaminate, it’ll just stop producing mushrooms & dry up after it’s completely spent its nutrients

s2b? i’m not familiar with that acronym but i understand what you’re asking for sure. i didn’t really notice an increase or decrease in the time from spawn to pins, & i don’t think i could say with any sort of certainty that it would be because of the bacteria even if i did see a difference as i haven’t been controlling for any variables (temperature has been all over the place, different genetics on each tub, some of them i fucked up & was out of town & couldn’t mist etc.) & honestly i haven’t had many clean tubs to compare them to lately as for some reason i’ve been struggling with bacterial spawn lately, which is why i made this journal in the first place. i wanted to track down what it was & document things on the way.
i have had some bacterial tubs take foreeeeeever to fruit in the past tho, even in warm conditions so i think it depends on the severity of the bacterial infection. i’d recon you would always get better yield out of healthy spawn vs bacterial spawn, though, so i think any potential time save with bacterial spawn would be negated by the finished yield


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OfflineAspiringmycologist
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Re: ??? [Re: hazyhorse]
    #27341889 - 06/09/21 04:31 PM (2 years, 7 months ago)

S2b=spawn to bulk. I’ve heard of some grains being more difficult for some and not so much for others(eg oats and corn) but I don’t have any personal anecdotes to support that. Wish I could help but regardless I hope you figure it out the cause. Keep us posted!


Edited by Aspiringmycologist (06/09/21 04:33 PM)


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Offlinehazyhorse
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Re: ??? [Re: Aspiringmycologist] * 1
    #27341901 - 06/09/21 04:36 PM (2 years, 7 months ago)

just realized i never really posted what grains i was using lol. i’m using oats!! some people really fuckin hate oats, but i know they can work really well so i’m trying to dial them in (i also have 50lb left of them so... i don’t have much choice lol.) i might try wbs just for the fuck of it one of these days tho. don’t sweat it tho man! i appreciate it. i’ll get it figured out soon. i’m gonna try some new methods to help rule out what it might be. one thing i think may have been a vector for contamination is that i was working in a really small SAB. my agar plates look fine for the most part (though there may be hidden bacteria- i’m going to try BRF agar pucks to make sure the spawn is fully clean this next run i do) & my jars never seem to get bacterial on their own after a PC cycle, so i’m thinking it must be coming from the way i’m inoculating the jars (unless it’s the agar of course)

i’ll definitely be posting in this thread a lot, so you’ll get to see me troubleshoot in real time lol


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Offlinehazyhorse
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Re: ??? [Re: hazyhorse]
    #27350775 - 06/16/21 01:59 PM (2 years, 7 months ago)

mysteryberry (spores):

you know the drill by now, grains pressed up against the glass, a lot of wetness in the jar, inconsistent myc etc.




update (6/7):

oops, totally forgot to snag a pic. i think i mixed up the rustywhyte & mysteryberry spores at some point, as this tub was white with rust colored spores. the spores almost looked gold, very beautiful. i should have taken a clone, but i took some spore prints i'll def be putting these to agar.


update (7/18):

started to trich out right after the second flush. might upload the pic later but i keep forgetting. it's not very exciting anyway


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Edited by hazyhorse (07/18/21 02:19 AM)


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Offlinehazyhorse
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PFC spores [Re: hazyhorse]
    #27350790 - 06/16/21 02:07 PM (2 years, 7 months ago)

PF classic (spores):

these jars are definitely bacterial, the shaken one didn't recover super well & is showing classic signs of bacteria at the bottom of the jar. the unshaken one was getting kinda fuzzy on the top & looks to be starting to pin. i let these jars sit for quite awhile so it makes sense. it looked a lot better than the shaken jar for sure, but still very bacterial.

shaken:


unshaken:



update (6/30):

got a pretty solid canopy from this tub!! i didn’t get the short & fat pf classic genetics but oh well. put the tub into its second flush, so we’ll see when it starts to contam out.



update (6/7):

tub is showing no sign of pinning & some spots of trich popping up. gonna toss it


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Edited by hazyhorse (07/07/21 02:34 AM)


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??? clone [Re: hazyhorse]
    #27392088 - 07/18/21 02:28 AM (2 years, 6 months ago)

mysteryberry clone:

so, i got a bigger SAB, & tried using josex's PF puck tek to try & rid this culture of bacteria. one plate is from a brown rice flower transfer (which should theoretically rid it of bacteria), while the other is just from a normal transfer. both were from the same starting plate of a grain from a jar of oats i'm keeping the culture on in my fridge.

PF agar puck:


bacterial:



the growth is clearly a bit different. the BRF transfer is looking much more irregular, but the transfer was from a different medium & was a bit messy. the one i think is bacterial has a ring in some of the earlier growth. i will use these plates to nocc up some jars, & i'll take a transfer from each to a new plate of agar to see if they grow similar, then probably put those plates to grain as well.


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Re: ??? clone [Re: hazyhorse] * 1
    #27393187 - 07/19/21 03:00 AM (2 years, 6 months ago)

I’m a noob but I think both plates look pretty good. I’ve read those rings in the mycelium can be caused by changes in temperature/ light cycles. I too need to invest in another sab. I’m getting more satellite contamination on my agar than I’m comfortable with


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Re: ??? clone [Re: Aspiringmycologist]
    #27398391 - 07/22/21 07:06 PM (2 years, 6 months ago)

look is the operative word there. all my agar plates look like this more or less, but i’m still getting bacteria in my grains, hence the thread. i’ve read the rings can be caused by non contam related things too, that didn’t necessarily concern me, but all my plates are kept in the same conditions on a stack, so i’m kinda curious as to why there would be one on one plate & not the other. i’ve had the same thing happen with other plates in the past too. i’m hoping i’ll be able to figure out if my agar is clean or not after that post

i feel you on the satellite colonies, that shit can be super frustrating. SAB size is definitely important, but i’ve worked in much smaller SABs (my first SAB for a long while was a 30qt LOL)& avoided those satellite colonies. i think a big thing for that is to make sure your hands never pass over anything open, as well as making sure any source of airflow in the room is off. bumping the SAB while you work can cause that shit too


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
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OfflineAspiringmycologist
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Re: ??? clone [Re: hazyhorse]
    #27398690 - 07/23/21 01:17 AM (2 years, 6 months ago)

I’ve heard of mycelium “meshing” with contaminants although I’d be lying if I said I knew more about it. A personal anecdote I can share though is I dropped grain on agar before and had it germinate no issues until after around the third transferred a week or so later. Every subsequent transfer i made from the germ plate and transfers would fail(the things would cover in bacteria). Ended up being really disappointing tbh but it’s part of the hobby haha. What’s funny is my “proto” sab was a trash bag propped like a tent with a can of Lysol(in my defense RR  has mentioned bags as being legit portable SAB haha) and I had no contam using that method fast forward and I put my 64 qt to use and mold is beating me up left and right lol


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Offlinehazyhorse
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Re: ??? clone [Re: Aspiringmycologist]
    #27399283 - 07/23/21 02:25 PM (2 years, 6 months ago)

yeah, mold can become interlaced with myc for sure, i’ve had the same exact thing you describe happen to me. usually i don’t get other molds meshed in unless i’m lazy about transfers on plates with satellite colonies, but the bacteria can be a sneaky ass bitch. i’ve been wondering if it’s my agar plates, my grain prep, or my SAB usage that is causing the bacterial grains. my agar looks fine for the most part but i feel like it’s more likely that than bad grain prep? idk we’ll see. i’ve been using josex’s PF puck tek to see if it was meshed with bacteria. i think if i’m still getting bacterial grains after that, i’ll try switching grains & prep to see

lmfaooooo that’s actually so wild. i was definitely doing shit like that when i started bc i was looking at hella old threads. that’s super dope you weren’t getting contams with it before, you’d think with the plastic being so easy to move you’d stir up contams & i haven’t seen that recommended at all on the last 7 years but clearly it worked. how big are the arm holes in your 64qt? i think arm hole size can play a factor. you want them really big so that moving your hands doesn’t create air currents


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