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ReverendMyc

Registered: 03/29/19
Posts: 1,580
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Hindsight] 1
#27184462 - 02/03/21 12:26 PM (3 years, 13 days ago) |
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Nice write up and info, Hindsight.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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Threads2theSoul
Inquirer


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27184698 - 02/03/21 02:59 PM (3 years, 13 days ago) |
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Quote:
Mr. Alien said:
Quote:
Threads2theSoul said:
... there is a TINY bit of sediment on the bottom of the HG ...
... Cook the LME ingredients using a pot, dissolve the malt extract in the water first, then add the agar agar... You should not have sediments if you do it that way.
I asked this in a different post but wanted to get your thoughts Alien...
I followed the above and this is what it looked like. You can see the cloudy sediment settled in the spoon and the pot is cloudy as well.
Should I try straining through a coffee filter? Would that decrease the nutritional value?
Or could it be the ingredients? I used Briess Dried Malt Extract (Pilsen Extra Light) and Telephone Agar Agar both from Amazon: https://www.amazon.com/gp/product/B00N0V289M/ref=ppx_yo_dt_b_asin_title_o06_s00?ie=UTF8&psc=1 https://www.amazon.com/gp/product/B085212JSH/ref=ppx_yo_dt_b_asin_title_o07_s00?ie=UTF8&psc=1

I've been using the agar anyway and seeing myc growth
I'm just trying for crystal clear agar...
I gave up on colors... first it disappeared after PCg, bought a different kind, that one kept the color after PCg but dissapeared when myc started to grow - guess the myc ate it up.
LOVE THIS TEK - Thank you Alien
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
#27189068 - 02/06/21 03:11 AM (3 years, 10 days ago) |
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Quote:
Threads2theSoul said: I've been using the agar anyway and seeing myc growth
I'm just trying for crystal clear agar...
I gave up on colors... first it disappeared after PCg, bought a different kind, that one kept the color after PCg but dissapeared when myc started to grow - guess the myc ate it up.
LOVE THIS TEK - Thank you Alien 
Myc always consumes colorant. Why you are on this quest to make crystal clear agar? as long as you don't have big chunks or big peaces of sediments, or also called "sharks", is fine. Agar with light malta extract will always have that light brown color, kind of like beer. Use more colorant, dark colorant like blue is better, because it lets you see the contrast of the white mycelium against the dark surface of the agar.
Quote:
karri0n said:
To be really fair, the clarity, size, and availability of three section dishes are absolutely advantages to petris over HG.
If I could get screw top petris that didn't need a wrap, I would. Needing Parafilm/plastic wrap is the real clincher for me.
If you could screw top real glass petris or disposable pre-sterilzied petris, but those solutions doesn't exist :P, they are not part of our reality we live in, so don't even torture yourself wishing they could exist. HG is the closest thing we have to that.
To be really fair in my opinion, people in this forum that still use disposable petri dishes instead of HG's because of the clarity or size in my opinion is just a whim attitude of... "i want to do it like the pro's" like if they were working in a lab, you don't need that much clarity to grow home mushrooms, or even to run a small gourmet/medicinal mushroom business. Seriously, using parafilm and pouring is a pain in the ass i don't want to go back, I'm lazy as fuck, and certainly i wouldn't go back to disposables because of that whim attitude I mentioned.
Quote:
cronicr said: considering you're already mixing pouring and pcing and fighting condensation
I do agree with that, don't fight the condensation, it defeats the lazy purpose of HG plates, just let time to de-condensate them. Honestly i get good results with the nutcracker container because i just put 10 HG's inside and sterilize them, and that's all i have to do. But then i see people in this thread using complicated methods to de-condensate them quick, some guy was even using a dehydrator, is just too much work, it defeats the purpose.
Edited by Mr. Alien (02/06/21 03:38 AM)
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cronicr



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27190091 - 02/06/21 05:11 PM (3 years, 10 days ago) |
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the price has sure sky rocketed hey they're on to us lol, it would cost me 30 bucks for 8 lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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AngioSporem
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: cronicr]
#27190198 - 02/06/21 06:24 PM (3 years, 10 days ago) |
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I tried using large Ball brand pickle jar (as suggested in some of the replies) and also Ball wide mouth quart jars, I had issues with the HG plates getting stuck and all being totally full of condensation (so much, with pools of liquid even after leaving lids cracked for a few weeks). Is my best bet to buy Kerr brand widemouth jars and try again? I tried leaving the HG plates cracked in front of my dehydrator while I was drying some fruits and all but two of the plates now show contam.
Edited by AngioSporem (02/06/21 06:28 PM)
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Mr. Alien
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Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: AngioSporem]
#27190271 - 02/06/21 07:05 PM (3 years, 10 days ago) |
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Quote:
cronicr said: the price has sure sky rocketed hey they're on to us lol, it would cost me 30 bucks for 8 lol
damn, haven't even seen the price, thankfully i foresee that and bought around 200 HGs when the thread was young.
Quote:
AngioSporem said: I tried using large Ball brand pickle jar (as suggested in some of the replies) and also Ball wide mouth quart jars, I had issues with the HG plates getting stuck and all being totally full of condensation (so much, with pools of liquid even after leaving lids cracked for a few weeks). Is my best bet to buy Kerr brand widemouth jars and try again? I tried leaving the HG plates cracked in front of my dehydrator while I was drying some fruits and all but two of the plates now show contam.
it could be bad sterilization, it could be that the lids were cracked way too much, it could be the dehydrator injected dirty air through the cracked lids. I would never use a dehydrator. Shouldn't get stuck on the wide mouth jars, at least not on mine. If they can fit easily inside the mason jar, they can come out easily as well, use the mason jar in the PC upside down, so they all come out easily.
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cronicr



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27190389 - 02/06/21 08:27 PM (3 years, 9 days ago) |
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dont try fight the condensation just prep ahead of time its really as simple as that even when i use petri dishes they are always waiting and ready i dont do shit right before i need it i just constantly do something
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Rhizomorph
Psychedelic Researcher



Registered: 04/24/20
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27190409 - 02/06/21 08:33 PM (3 years, 9 days ago) |
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Quote:
Mr. Alien said: If you could screw top real glass petris or disposable pre-sterilzied petris, but those solutions doesn't exist :P, they are not part of our reality we live in, so don't even torture yourself wishing they could exist. HG is the closest thing we have to that.
I'm gonna torture myself by wishing and say that somebody somewhere could definitely make glass petris lined with PP5 plastic ribs making them able to function the same way the HG plates do.
All the benefits of petris and HG plates combined. I'd kill to get my hands on something so cheap and simple... Any manufacturers looking to make some cash?
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Mr. Alien
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Registered: 01/14/14
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
#27190442 - 02/06/21 08:46 PM (3 years, 9 days ago) |
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Quote:
Rhizomorph said: Any manufacturers looking to make some cash? 
I'll even crowdfund this as a major investor, i want to make some good money, i know y'all buy the new design. PP6 is a good alternative, is much more clear than pp5 and it is also autoclavable.
Look at the clarity of this thick pp6 flask against my arm:

Honestly i would prefer pp6 than glass.
Edited by Mr. Alien (02/06/21 11:26 PM)
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cronicr



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27190568 - 02/06/21 09:54 PM (3 years, 9 days ago) |
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 no pour 10 days after sterilization
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: cronicr]
#27190731 - 02/06/21 11:30 PM (3 years, 9 days ago) |
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cronicr



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27191625 - 02/07/21 12:23 PM (3 years, 9 days ago) |
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the were at 18 plus 10 to ship to Canada, I'm still not paying 25 for 8 lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Josex
#cheat_code


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: cronicr]
#27191630 - 02/07/21 12:26 PM (3 years, 9 days ago) |
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Yea lol I've seen borosilicate petris cheaper than that in Alibaba from chinese vendors.
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cronicr



Registered: 08/07/11
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Josex]
#27191651 - 02/07/21 12:35 PM (3 years, 9 days ago) |
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I have polypropylene petris they're ok for the first few transfers then I switch to glass or disposables
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: cronicr]
#27192130 - 02/07/21 03:45 PM (3 years, 9 days ago) |
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Quote:
cronicr said: the were at 18 plus 10 to ship to Canada, I'm still not paying 25 for 8 lol
Yeah, same for Mexico ONE 8pack shipping is 10$, but for TEN 8pack's is about 37$ shipping to Mexico. So if you order 10 8packs it would be 3.7$ of shipping for each 8pack.
Bulk order is more cost efficient.
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Sankhara
Trump's lost child


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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27193138 - 02/08/21 06:41 AM (3 years, 8 days ago) |
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I wish i had containers like this in Argentina.
Shipping tends to cost a fortune
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Psicomb



Registered: 01/13/18
Posts: 4,662
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Sankhara]
#27193301 - 02/08/21 09:14 AM (3 years, 8 days ago) |
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Just ordered 24 of these. I've used them once before and they're really wonderful. Thanks for the tek, alien
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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something - nick sand
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kidcharlemagne
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Psicomb]
#27193600 - 02/08/21 11:52 AM (3 years, 8 days ago) |
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So rather than stack these high, why not just stack them wide all wrapped in some towels (or other insulator) and put an equivalent amount of water in a pan at the top? A glass jar is hardly an insulator. Take the PC off the heating element after it's done so there's no extra residual bottom heat. And you should have equal thermal masses above and below. What am having missing?
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karri0n
Mind Traveller



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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
#27194559 - 02/08/21 11:59 PM (3 years, 7 days ago) |
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Quote:
Mr. Alien said: If you could screw top real glass petris or disposable pre-sterilzied petris, but those solutions doesn't exist :P, they are not part of our reality we live in, so don't even torture yourself wishing they could exist. HG is the closest thing we have to that.
To be really fair in my opinion, people in this forum that still use disposable petri dishes instead of HG's because of the clarity or size in my opinion is just a whim attitude of... "i want to do it like the pro's" like if they were working in a lab, you don't need that much clarity to grow home mushrooms, or even to run a small gourmet/medicinal mushroom business. Seriously, using parafilm and pouring is a pain in the ass i don't want to go back, I'm lazy as fuck, and certainly i wouldn't go back to disposables because of that whim attitude I mentioned.
I do agree with that, don't fight the condensation, it defeats the lazy purpose of HG plates, just let time to de-condensate them. Honestly i get good results with the nutcracker container because i just put 10 HG's inside and sterilize them, and that's all i have to do. But then i see people in this thread using complicated methods to de-condensate them quick, some guy was even using a dehydrator, is just too much work, it defeats the purpose.
I don't want them because I want to be like the pros. I can see that what the pros are doing doesn't fit my needs, because theyre using a design that doesn't take advantage of a complex path like threads, necessitating extra costs and steps of using parafilm.
I don't have real petris and I want them because, while I can see through the HG's, my cell phone camera really can't very well, and I like posting pictures on mushroom forums.
I want the size because sometimes having that extra space is the difference between saving a wild clone culture and losing it, or transferring contam needing another transfer basically wasting your work.
There are plastic manufacturing companies, and psychedelics are both spiking in popularity and well on their way to being legal. It's not actually an impossibility to get true "holy grail" screw containers at 100mm made of pp6 produced.
We could even do something like make it a much more aggressive thread that only requires a single turn or half turn, to minimize movements in the SAB.
What was the cost for the pp6 flask?
Quote:
kidcharlemagne said: So rather than stack these high, why not just stack them wide all wrapped in some towels (or other insulator) and put an equivalent amount of water in a pan at the top? A glass jar is hardly an insulator. Take the PC off the heating element after it's done so there's no extra residual bottom heat. And you should have equal thermal masses above and below. What am having missing?
Sounds like the only thing missing is for you to try it and report back whether it worked.
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Panaeolus Bisporus
Edited by karri0n (02/09/21 12:01 AM)
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Mr. Alien
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Registered: 01/14/14
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
#27194646 - 02/09/21 01:06 AM (3 years, 7 days ago) |
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Quote:
karri0n said:
I don't want them because I want to be like the pros. I can see that what the pros are doing doesn't fit my needs, because theyre using a design that doesn't take advantage of a complex path like threads, necessitating extra costs and steps of using parafilm.
I don't have real petris and I want them because, while I can see through the HG's, my cell phone camera really can't very well, and I like posting pictures on mushroom forums.
I want the size because sometimes having that extra space is the difference between saving a wild clone culture and losing it, or transferring contam needing another transfer basically wasting your work.
There are plastic manufacturing companies, and psychedelics are both spiking in popularity and well on their way to being legal. It's not actually an impossibility to get true "holy grail" screw containers at 100mm made of pp6 produced.
We could even do something like make it a much more aggressive thread that only requires a single turn or half turn, to minimize movements in the SAB.
Quote:
I don't want them because I want to be like the pros. I can see that what the pros are doing doesn't fit my needs, because theyre using a design that doesn't take advantage of a complex path like threads, necessitating extra costs and steps of using parafilm.
WISE WORDS, also time is money, so pouring count as extra costs.
About the size, yeah, true, wider would be much better and it would make the difference for saving a wild clone culture.
About taking good picture, i mean is possible, eatu took some real good pictures with a nice lighting and nice camera. But that would be the best picture you could take.

Quote:
We could even do something like make it a much more aggressive thread that only requires a single turn or half turn, to minimize movements in the SAB.
Idk tho, it does need to have enough threads to be considered an obscure pathway to make it harder for contams to find its way in no? i really don't know, maybe is possible with less threads
I can't remember the price of the flask.But i think pp6 is more expensive than pp5.
Edited by Mr. Alien (02/09/21 01:34 AM)
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