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InvisibleAtomsplit
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Registered: 01/16/21 Happy 4th Shroomiversary!
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
    #27587671 - 12/19/21 07:17 PM (3 years, 28 days ago)

Quote:

Smartattack said:
I like millet, but I also use the grey plastic ball lids and it drives me fucking bananas how so many seeds end up in all the nooks and crannies of the lid. 😡




I'm going to start using the standard 2 piece metal lids when working with millet.

That should help with the rogue :dwarf: millet grains that get stuck in the threads of the lid & jar.

Just curious cause those rogue grains never seemed to have caused any serious problems that I'm aware of.


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InvisibleStipe-n CapMDiscord
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Re: Clean spawn checklist for the new grower. [Re: Atomsplit]
    #27587676 - 12/19/21 07:19 PM (3 years, 28 days ago)

I never have an issue with them getting in the threads, and the ones that stick to the inside will fall after you shake to grains while it's colonizing.

I find pouring from bags to be awkward so I still use jars for masters.

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Invisiblebigfootscreepyuncl
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27587678 - 12/19/21 07:25 PM (3 years, 28 days ago)

I don't want to turn this thread into a preferred grain or prep TEK, but do you use millet as your spawn as well, or just for masters? In another millet prep thread I was reading people were saying it doesn't work well as spawn because it produces less fruit than other grains? If you're running bags of it I can't imagine that's the case, though.

Sorry for getting off topic!


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InvisibleStipe-n CapMDiscord
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Re: Clean spawn checklist for the new grower. [Re: bigfootscreepyuncl] * 3
    #27587681 - 12/19/21 07:28 PM (3 years, 28 days ago)

I use it for spawn, whoever said those things is a trick ass noob.

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InvisibleMr. Alien
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Registered: 01/14/14
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
    #27587693 - 12/19/21 07:43 PM (3 years, 28 days ago)

Quote:

p9hu7 said:

About to load into the pc:




Fresh out of the PC:








Damn man, you load them up 2/3, and they came out of the PC at 2/3 too? After soaking, when I do that, my millet expands.

Quote:

Smartattack said:
I like millet, but I also use the grey plastic ball lids and it drives me fucking bananas how so many seeds end up in all the nooks and crannies of the lid. 😡




Me to man, drives me crazy, I will use metal lids. Also, I was thinking of using some millet at the bottom of the mason jar and fill the rest with wheat, to avoid that issue, and still get the inoc points of millet.

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OfflineSpaceBaby
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Re: Clean spawn checklist for the new grower. [Re: Mr. Alien]
    #27587716 - 12/19/21 07:55 PM (3 years, 28 days ago)

i just got grey plastic lids. i like them SO much better than 2-pc metal. so far millet has been my most successful work. oats are kicking my ass, but tomorrow i am gonna wash the new premium race horse oats before i run them. i did run 16 jars of millet today. nocc'd 7 jars of millet with reishi LC.

oh boy IFLM.


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OfflineHindsight
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Re: Clean spawn checklist for the new grower. [Re: SpaceBaby]
    #27587780 - 12/19/21 08:55 PM (3 years, 28 days ago)

Alien, another millet prep method you can try (I didn't come up with it) is to 12 hour soak, then drain, then load into a pot, *slowly* bring to a simmer while stirring (7 grain jars worth should take around 20-30 mins to reach simmer) and as soon as it reaches a simmer, dump them in a strain bag and hang it up for 12 hours. Then load into jars and PC. It does take more time end-to-end and you have to be around to stir the simmer, neither of which I like, but I can't match the performance of the grain with any other prep methods, personally.

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InvisibleMr. Alien
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Re: Clean spawn checklist for the new grower. [Re: Hindsight]
    #27587834 - 12/19/21 10:43 PM (3 years, 28 days ago)

Quote:

Hindsight said:
Alien, another millet prep method you can try (I didn't come up with it) is to 12 hour soak, then drain, then load into a pot, *slowly* bring to a simmer while stirring (7 grain jars worth should take around 20-30 mins to reach simmer) and as soon as it reaches a simmer, dump them in a strain bag and hang it up for 12 hours. Then load into jars and PC. It does take more time end-to-end and you have to be around to stir the simmer, neither of which I like, but I can't match the performance of the grain with any other prep methods, personally.




Thank you for that method, I will try it, I prefer simmer, it gives me more peace of mind that the core of the millet is more cooked and therefore easier to sterilize, less possibility of bacteria I guess. Who came up with this method? Is there a TEK somewhere?

And you load the jars 2/3? Or they will expand more in the PC?

So as soon as I start to see bubbles on the boiling water of the pot, I turn off the stove and dump it into the strain bag? Not even 1 minute of boiling water?

I'm okay with more work, if the results are better.

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OfflineHindsight
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Re: Clean spawn checklist for the new grower. [Re: Mr. Alien]
    #27587983 - 12/20/21 06:40 AM (3 years, 27 days ago)

JakeOnCid came up with that method, specifically for Pans (which is 90% of what I’ve been growing for a while), but of course, it works great with any mushrooms. Pans are just much more finicky about grain hydration so you have to be 100% on point for them to work. He hasn’t posted a Tek for it, just outlined it to me in response to a post I made on the pan thread when I was having a lot of issues with stalled jars.

The method calls for 1 part rye to 3 parts millet. I have tried with that, and also with pure millet. Both work but adding rye does seem to help. I worried about uneven hydration of the rye given the size difference between rye and millet but all I can say is it works so I quit worrying.

The jars do expand a bit but not much. I load them about 2.5 cups each. As for boiling, I have found a minute or two either way doesn’t matter much. I wait until I see bubbles across most of the surface even after I stir them, dump into strain bag and hang up for 12 hours. Sometimes I soak overnight, simmer in morning, then PC at night and sometimes I soak all day, simmer at night and PC in morning.

One thing JOC said is, after drying ~12 hours in the strain bag, he will dump the grains and spread them out on a tray and if needed, allow to dry until a bunch of grains don’t stick to your hand when you run your hand through them. This is similar to what BOD uses as a dryness test for his oat prep Tek but whether I’m using BODs method or JOCs, if I get my grains that dry before PCing, my jars nearly always stall from the grains drying out too much. I believe your growing room humidity and your grain jar lid (and filter type) choice factors into how damp your jars need to be in order to avoid them losing too much moisture during the ~14 or so days of colonization so you’ll want to play with that part yourself to dial it into your own conditions and setup.

This method has a fair amount of variability in things you can change up so it’s definitely one you’ll want to play with a bit and start small on but it gives me the best success even when I change things up on purpose or accident. Knowing when to pull from simmer is the least scientific (and so most frustrating for me) part but I haven’t made a bad batch yet.

Edited by Hindsight (12/20/21 04:04 PM)

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InvisibleMr. Alien
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Re: Clean spawn checklist for the new grower. [Re: Hindsight]
    #27588502 - 12/20/21 04:03 PM (3 years, 27 days ago)

I will try this thanks you.

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OfflineHindsight
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Re: Clean spawn checklist for the new grower. [Re: Mr. Alien]
    #27588506 - 12/20/21 04:05 PM (3 years, 27 days ago)

No problem. Major typo I just corrected in my last post:

This: 1 part grain to 3 parts rye
Should be this: 1 part rye to 3 parts millet

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InvisibleInfinite Monkeys

Registered: 09/01/21
Posts: 276
Re: Clean spawn checklist for the new grower. [Re: Hindsight]
    #27588560 - 12/20/21 04:38 PM (3 years, 27 days ago)

This has probably been asked before but can anyone suggest some gloves to use? Preferably reusable/washable.


Using bare hands is a barrier to axenic cultures/spawn.

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InvisibleMysticMycologist
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Re: Clean spawn checklist for the new grower. [Re: Infinite Monkeys]
    #27588561 - 12/20/21 04:40 PM (3 years, 27 days ago)

I use yellow kitchen gloves, dishwasher after use (top shelf) and rub with ISO before use. They’ve lasted quite a while, and I like how they go kind of far up my arms. Get tight fitting ones for dexterity.


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InvisibleInfinite Monkeys

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Re: Clean spawn checklist for the new grower. [Re: MysticMycologist] * 1
    #27588855 - 12/20/21 09:31 PM (3 years, 27 days ago)

Quote:

MysticMycologist said:
I use yellow kitchen gloves, dishwasher after use (top shelf) and rub with ISO before use. They’ve lasted quite a while, and I like how they go kind of far up my arms. Get tight fitting ones for dexterity.




Thanks.

I just tried using kitchen gloves and the large side was way too big.

The medium size will be difficult to get my hands into but as you said, the tight fitting ones have the benefit of dexterity.

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InvisibleTedsDead
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Re: Clean spawn checklist for the new grower. [Re: Infinite Monkeys]
    #27589017 - 12/21/21 12:55 AM (3 years, 27 days ago)

You can reuse latex gloves over and over untill they rip


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Offlinetedoro
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27590368 - 12/22/21 08:15 AM (3 years, 25 days ago)

Quote:

p9hu7 said:

Do you rinse/clean your millet before soaking? If I soak millet without washing like other grains I'm guaranteed to get bacteria.






What do you suspect causes problems with no rinsing?


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InvisibleStipe-n CapMDiscord
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Re: Clean spawn checklist for the new grower. [Re: tedoro]
    #27590371 - 12/22/21 08:18 AM (3 years, 25 days ago)

Allowing the overburden to remain with your grains means that you are reducing the efficiency of your sterilizer/cycle to penetrate the load, resulting in  partial destruction of bacterial endospore. Higher likelihood of incomplete sterilization.

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InvisibleStipe-n CapMDiscord
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Re: Clean spawn checklist for the new grower. [Re: Anonymous]
    #27590404 - 12/22/21 08:39 AM (3 years, 25 days ago)

I borrowed the terminology from gold prospecting, gold lies on the basement granite bedrock covered in river stones, earth, sticks, etc: this is overburden.

Same as with grain, your grain surface is the target of sterilization but it's completely covered in the overburden of bran, dirt, sticks, etc which contains a bioburden. Bioburden is the biological burden that requires sterilization, that burden varies from grain type to grain type, and the amount of dirt etc thats present.

When running your autoclave it's important to consider what will act as a barrier to efficiency of the heat energy to penetrate the materials that you're working on. If there is an abundance of filth and dirt it will reduce the efficient penetration of all of those materials, so by washing it off you are removing barriers to sterility.

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InvisibleStipe-n CapMDiscord
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27590405 - 12/22/21 08:40 AM (3 years, 25 days ago)

That's weird, the comment I was responding to is now gone, lol.

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Offlinetedoro
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27590407 - 12/22/21 08:42 AM (3 years, 25 days ago)

Quote:

p9hu7 said:
That's weird, the comment I was responding to is now gone, lol.




I was embarrassed after googling it, felt like I was abusing my P9 access~

I asked what the term overburden meant.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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