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InvisibleSmartattack
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Re: Clean spawn checklist for the new grower. [Re: Atomsplit]
    #27308998 - 05/14/21 08:38 PM (3 years, 7 months ago)

Yeah those look nasty AF. I'd probably go WBS at that point.


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InvisibleStipe-n CapMDiscord
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Re: Clean spawn checklist for the new grower. [Re: Atomsplit]
    #27309003 - 05/14/21 08:41 PM (3 years, 7 months ago)

Quote:

Atomsplit said:
I agree! I've bought rye berries from 3 or 4 sources and only one source (unfortunately the most expensive) has barely any cut grains.

These cut grains make it very difficult to not have burst grains so don't rely on some burst grains that your grains are done





Well I have good news for you then, burst grains aren't how you determine when your grain is hydrated.

So when you look at the pictures above and see the white core that is how you tell if your grain is hydrated, by cutting it open and checking to see how translucent the core has become. When the white is gone, they're done. Some grains will burst but that's not what you're looking for anyways.

That's why I don't personally like the soak method for large grains, because it fails to completely hydrate the core of the grain.


Edited by Stipe-n Cap (05/14/21 09:01 PM)

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OfflineMrs. Doubtfire
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Re: Clean spawn checklist for the new grower. [Re: Mrs. Doubtfire]
    #27309074 - 05/14/21 09:36 PM (3 years, 7 months ago)

Quote:

Mrs. Doubtfire said:
How we looking? Agar to rye, nearly 7 days in.




Jar 1


Jar 2






Couldn't resist shaking these about ~4 days ago, they recovered 1-2 days later and here they are now...fuck if I know.


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InvisibleStipe-n CapMDiscord
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Re: Clean spawn checklist for the new grower. [Re: Mrs. Doubtfire]
    #27309084 - 05/14/21 09:43 PM (3 years, 7 months ago)

It's honestly hard to say, but it looks like there's mold on the grains top center  on the second jar.

Pictures aren't always easy to diagnose. The first pic looks better than the second  one.

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OfflineMarkamello
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27309536 - 05/15/21 08:38 AM (3 years, 7 months ago)

Great thread, this is going to have me go over all my technique with a fine toothed comb.

I have been having a lot of bad luck with contam and now think I can put it down to using bacterial spawn.

I use millet and or milo. I put 1300ml of millet in the bag add 700ml of water and load them straight into my 44 gallon drum steriliser. I run the bags for 14hrs at 101°c. I work in front of a flow hood and inoculate the bags using lc pre-tested on agar then expand the grain out to more bags. I have a lot of success but an equal amount of failure.

I suspect that all these are bacterial. Can someone please confirm?




I hope that this is one is okay. A have several bags that look like this (they all came from the same run) I will use these as master grain bags to expand out into 40 more bags. Again can someone please confirm?


I will save myself a lot of wasted hard work and time when I learn to ID bacteria early

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InvisibleSmartattack
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Re: Clean spawn checklist for the new grower. [Re: Markamello]
    #27309548 - 05/15/21 08:44 AM (3 years, 7 months ago)

Looks like the thread has taught you well.:dancer:


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OfflineMarkamello
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27309569 - 05/15/21 08:58 AM (3 years, 7 months ago)

Thanks for the confidence booster. To be clear though, the last pic looks the goods, yeah?

Now I just hope that finding the flaws in my sterile technique is as easy as identifying bacterial spawn.

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OfflineA.k.aM
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Re: Clean spawn checklist for the new grower. [Re: Markamello]
    #27309605 - 05/15/21 09:31 AM (3 years, 7 months ago)

Last one looks on point to me.


Honestly though I’d be running every one of those. I’d bet you get at least a solid flush from all of them.


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InvisibleSmartattack
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Re: Clean spawn checklist for the new grower. [Re: A.k.a]
    #27309626 - 05/15/21 09:42 AM (3 years, 7 months ago)

Me too. I like to look at spawn to check it's health, but unless really bad I just run most everything anyways lol. Bad stuff can surprise. I think it's most important to just know what not to use as master grain.


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* Smarts videos :teacher:
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InvisibleAtomsplit
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
    #27312072 - 05/17/21 03:18 AM (3 years, 7 months ago)

Quote:

Smartattack said:
Yeah those look nasty AF. I'd probably go WBS at that point.




Yes that bag of rye looks bad. What do you think about these ingredients for wbs? Milo, White Proso Millet, Wheat, Sunflower Seed?

I can get this wbs for a lot cheaper than rye but with all these different seeds I cant see the prep being too easy.

Thank u



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OfflineStonedCatRescue
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Re: Clean spawn checklist for the new grower. [Re: A.k.a]
    #27312162 - 05/17/21 06:00 AM (3 years, 7 months ago)

Quote:

A.k.a said:
Wbs and millet can get pretty chunky even with healthy myc.  I bet if you let that jar sit for another ten days you’d have to spoon it out. :lol:




This is a great thread. I've been sat here reading it for days now in between other jobs. It's totally opened my eyes to the whole grain spawn health with the attention to the nuances which aren't really mentioned elsewhere.

I do still have a few questions and this is one of them..

What about rice? Every tek video involving rice I've seen on youtube by presumably experienced growers, seems to be a solid block of white mass. Bacterial?

And there are a few points where P9 says to shake the jar to check the myc... but what if you have a jar or bag which is bacterial which when shook bounces back to white again? Can bacterial myc continuously bounce back after multiple shakes?

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OfflineA.k.aM
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Re: Clean spawn checklist for the new grower. [Re: StonedCatRescue]
    #27312192 - 05/17/21 06:37 AM (3 years, 7 months ago)

Yeah some bacteria won’t totally stop myc and a jar will recover, but most of the time if you shake a bacterial jar you’ll end up with a wet mess a week later.


I’ve never used rice but I know what you’re talking about. It’s so small and soft that it colonizes into a block from what I’ve seen. Anything you don’t shake will eventually do that though.


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InvisibleAtomsplit
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Re: Clean spawn checklist for the new grower. [Re: A.k.a]
    #27313936 - 05/18/21 03:15 PM (3 years, 7 months ago)

Thanks for the advice on the 30% shake AKA.

I guess my rye jars aren't doing well if they don't completely break apart at 30%.

Even after a bunch of good smacks :sasquatch: I'm left with a piece like this.



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OfflineStonedCatRescue
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Re: Clean spawn checklist for the new grower. [Re: A.k.a]
    #27314106 - 05/18/21 05:53 PM (3 years, 7 months ago)

Quote:

A.k.a said:
Yeah some bacteria won’t totally stop myc and a jar will recover, but most of the time if you shake a bacterial jar you’ll end up with a wet mess a week later.


I’ve never used rice but I know what you’re talking about. It’s so small and soft that it colonizes into a block from what I’ve seen. Anything you don’t shake will eventually do that though.





Cheers.

This is where this thread needs just a bit of padding out for the newbie like me. Some clarification about these fine points to really separate what's ok from what's not ok.

So let's say you had a jar/bag which you Id as possibly bacterial, but you break it up and it bounces back but it still has the same spikey growth showing, then you break it up again and it bounces back in 24hrs but with signs - based on the info in this thread - that it's possibly/likely bacterial, then what? 

To a newb, explain what you would take from that above scenario?

And also, regarding metabolites, would a perfectly healthy bag/jar produce a lot of liquid if left to sit for a couple of months after full colonisation?

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OfflineGan
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Re: Clean spawn checklist for the new grower. [Re: StonedCatRescue]
    #27314249 - 05/18/21 08:29 PM (3 years, 7 months ago)

Depends what you're using that jar/bag for. If it's just to spawn it, then sure I'd say it would still be good to spawn. Although I'd make sure to take pictures and document well to be able to go back and reference if it turns out to have issues.

Would I use that jar for g2g? Nope. I don't use jars I'm not 100% about to g2g. So even if they recover well, if they're looking off in the visual department I'm saying nope. To g2g I need it to pass all all of my clean spawn checks without question


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InvisibleSmartattack
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Re: Clean spawn checklist for the new grower. [Re: Gan]
    #27314346 - 05/18/21 10:22 PM (3 years, 7 months ago)

Quote:

Gan said:
Depends what you're using that jar/bag for. If it's just to spawn it, then sure I'd say it would still be good to spawn. Although I'd make sure to take pictures and document well to be able to go back and reference if it turns out to have issues.

Would I use that jar for g2g? Nope. I don't use jars I'm not 100% about to g2g. So even if they recover well, if they're looking off in the visual department I'm saying nope. To g2g I need it to pass all all of my clean spawn checks without question





:whathesaid:

I think we are really trying to stress not that you cant use questionable spawn but:

A: Do not use questionable spawn for expansion. G2G Etc.

B: Do not be surprised by less than stellar results if spawned.

In a nutshell, dont multiply anything not perfect, but spawn it anyways unless it is definitely bad.


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OfflineA.k.aM
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
    #27314644 - 05/19/21 06:17 AM (3 years, 7 months ago)

My general rule is I’ll spawn anything that finishes colonizing, unless it’s absolutely soaked at the bottom.


I’ve had surprisingly good flushes from bacterial, yeast, and twice jars with small spots of brownish mold.



Like smart said a lot of contam spawn will work, it’s just usually a one flush tub.


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InvisibleAtomsplit
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Re: Clean spawn checklist for the new grower. [Re: A.k.a]
    #27315147 - 05/19/21 01:36 PM (3 years, 7 months ago)

Thank you Gan Smartattack & AKA for your responses

Mush cult can't be something that the average person can "wire" in a few months.


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OfflineIrishmule169
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Re: Clean spawn checklist for the new grower. [Re: Atomsplit]
    #27316885 - 05/20/21 08:11 PM (3 years, 7 months ago)



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OfflineNunnamaker
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27316937 - 05/20/21 09:36 PM (3 years, 7 months ago)

Great write up,look forward to learning a lot from all of you!

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