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clockworkshroom
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Registered: 02/20/20
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27195063 - 02/09/21 08:54 AM (2 years, 11 months ago) |
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Quote:
p9hu7 said: The reason that millet can be loaded wet is that its a small, hard seed. Any excess surface moisture is going to be absorbed during the cycle, it hasn't had water already boiled into it the same way larger grains like oats do. This will happen in the PC.
Large cereal grains have been plumped up and hydrated by boiling so they're basically at grain "field capacity ". If they don't dry you'll get excessive moisture. This excess moisture, as outlined in the OP, will exploit any shortcomings in sterile procedure.
I really should take basic science again before bothering you all with questions. Thanks as always
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
#27195077 - 02/09/21 09:04 AM (2 years, 11 months ago) |
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@tedoro No difference so far as I can tell.
@clockworkshrooms Thats what this thread is for, so that new growers can get a better understanding of what's going on with their spawn. The world benefits from us engaging with mushrooms. My hopes are that I'll help to bring more mushrooms into the world by helping you guy's troubleshoot your bacteria. Win win.
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tedoro
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27195496 - 02/09/21 12:48 PM (2 years, 11 months ago) |
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I've been thinking about something. Have you guys ever hydrated chia seed for a chia pet? You know that slime that occurs? I've wondered if at a much more subtle way, some of these grains have some slime and/or starch that could keep the outer kernel more damp than if the kernels were to be cleaned off just prior to drying. I'm not much of a grain rinser. But I'm going to start, just for giggles.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: tedoro]
#27195508 - 02/09/21 12:55 PM (2 years, 11 months ago) |
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Wheat is covered in bran which gets rinsed off. Oats are dirty af and I always washed those as well, I don't rinse millet. That being said: all grains have startch, your grains will be startchy on the exterior when overcooked and will have a solid white/starchy core when undercooked. Faht runs some starchy grains as mentioned in an earlier reply, the mycelium seems to dig it.
Like smart said, properly drying your grains will keep them from having excess moisture. Cleaning dirty grains before cooking them is probably a good idea.
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Smartattack
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27195699 - 02/09/21 02:51 PM (2 years, 11 months ago) |
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I need to make a confession here. I realised I have never shared this before as it has been standard op for me for some time. I actually wash my oats by adding about 3-4 drops of dish detergent, then agitating vigorously for a few minutes. Not only does it clean them fantastically but I quickly noticed that I'm guessing due to the surfactant breaking tension - the oats hydrate noticably faster and before bursting.
I do a wash, then 2 simple rinses. There has been zero negative effect on culture speed or health in doing this.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
Edited by Smartattack (02/09/21 02:58 PM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
#27195711 - 02/09/21 02:57 PM (2 years, 11 months ago) |
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Seems like a good idea. Its one of those simple things that just hadn't crossed my mind. Thanks for sharing.
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Lenz
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Registered: 08/17/20
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27195930 - 02/09/21 05:14 PM (2 years, 11 months ago) |
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Somewhat bacterial APE jar on the left vs cleaner APE jar on the right.
 
Don't know if the jar on the right is completely clean though, if you look at the top left there's some slight wetness. It doesn't really look like bacterial wetness but just some slight liquid or water between the glass and the colonized mass of grain.
Anyone know if this is actually indicative of bacteria or if it's just natural condensate? My jars also get a decent amount of sunlight here and there so I'm thinking that could result in more condensation as well.
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Stipe-n Cap


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Re: Clean spawn checklist for the new grower. [Re: Lenz]
#27195940 - 02/09/21 05:20 PM (2 years, 11 months ago) |
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There are other symptoms present in those jars other than moisture. Both jars have bacteria.
Can you spot the issues?
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Lenz
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Registered: 08/17/20
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Loc: Fungistan
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27195973 - 02/09/21 05:37 PM (2 years, 11 months ago) |
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I honestly can't really, at least for the right jar. Left jar has that classic creamy and spikey dense mycelium that'll be a bitch to break apart but on the right I can't see much besides the condensation.
I've usually considered jars that look like the one on the right to be pretty clean, they don't look as pretty as some of the pictures in the OP but they break really easily, uniform color, nice wispy growth.
Perhaps it's the density of the growth not being quite uniform?
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: Lenz]
#27196028 - 02/09/21 06:01 PM (2 years, 11 months ago) |
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With the second jar you can see how the mycelium is behaving differently in different areas of the jar. There are many grains that have thick white mycelium radiating out from individual grains. Sometimes these grains are grouped together densely while others are not.
The mycelium will have uniform growth throughout your grains when it has free reign. Where mycelium is fighting bacteria you'll see the mycelium thicken in response.
Notice the uniformity in density and colour in these following pics:
  
Variation in density and colour is abnormal behavior for healthy mycelium.
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Lenz
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27196065 - 02/09/21 06:20 PM (2 years, 11 months ago) |
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Ahhh, yeah I see exactly what you mean, even with the thicker growth radiating out from the individual grains. Damn that is a bummer but really helpful. No idea where to start with this, could be my filters or my sterile tek but regardless thanks yo.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: Lenz]
#27196067 - 02/09/21 06:21 PM (2 years, 11 months ago) |
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Have you looked at the updated OP? There is another prep checklist at the bottom.
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clockworkshroom
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27196106 - 02/09/21 06:36 PM (2 years, 11 months ago) |
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Hey P9, your middle jar kind of looks a bit irregular to me (with my extremely limited experience), is that due to the grain type? Or the fact it's non fully colonised yet? It looks like there are small sections the myc isn't touching and some grains have really thick growth centred on them which you said was a warning sign. Obviously not questioning whether you are right or not just want to understand as best possible what to look out for.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
#27196112 - 02/09/21 06:38 PM (2 years, 11 months ago) |
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That middle pic is just recovering from a shake and is doing so beautifully.
There is a portion near the top that looks like what you describe but its an illusion caused by the corner of the glass and the light.
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Sold Out Online
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27196802 - 02/10/21 05:45 AM (2 years, 11 months ago) |
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Using the checklist, I think I might have a clear jar here.

It isn’t thick and milky.
It isn’t spiky.
There’s no excess of moisture.
No uncolonized grains pressed up against the glass looking wet, sad, and alone.
It’s Whyte Gold. If the doctor gives the all clear I guess I’m going G2G and I know when my next grow will be!
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Threads that have been useful to me: Clean Spawn checklist for new growers
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Stipe-n Cap


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Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: Sold Out Online]
#27196892 - 02/10/21 07:19 AM (2 years, 11 months ago) |
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She looks clean. I'd give it another day though just to be sure it's ready. Oats always look weird to me.
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TheBoJim
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27197841 - 02/10/21 05:32 PM (2 years, 11 months ago) |
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So bad they won’t even top fruit
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Sockadin



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Re: Clean spawn checklist for the new grower. [Re: TheBoJim]
#27197861 - 02/10/21 05:41 PM (2 years, 11 months ago) |
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Im loving this write up P9hu7. This makes me want to go back and do more Safflower and Black Seed. I have to say they were some of the easiest grains to prep and also had a cool novelty aspect to the jar growth.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: TheBoJim]
#27197884 - 02/10/21 05:54 PM (2 years, 11 months ago) |
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Quote:
TheBoJim said: So bad they won’t even top fruit

Give it time, I see knots on the side.
@sock
Yeah they definitely look cool. I've never used it but the contrast makes the mycelium look pretty sweet.
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tedoro
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27198912 - 02/11/21 09:24 AM (2 years, 11 months ago) |
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P9,
Might I ask your thoughts on the cause and implications of uncolonized kernels on the very top surface of a bag or jar? I have read that someone on the forum had collected some of the kernels and placed them on agar and proved them to be sterile. But still, they are uncolonized and seem problematic.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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