Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >
Offlineclockworkshroom
Stranger
 User Gallery

Registered: 02/20/20
Posts: 655
Last seen: 2 years, 9 months
Re: Clean spawn checklist for the new grower. [Re: Gan]
    #27191286 - 02/07/21 09:28 AM (3 years, 1 month ago)

I have plates that have sat for a month and are crystal clear still, I also have colonised plates that are 6 weeks old, overgrown and have shown no visible contamination so I don't think my agar work is the issue.

Do you think bacteria would have shown on my grain jars from the op if myc wasn't present or sometimes is it only visible if myc grows too? I like the suggestion of leaving a jar non inoculated, I'll definitely do that next time.


--------------------
p9hu7's clean spawn thread

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
    #27191287 - 02/07/21 09:30 AM (3 years, 1 month ago)

Try making transfers from those blank dishes into your sterilized jars to test your technique. You are covered in bacteria and mold, you're the likely the source of your contamination.

This is how your grain should look:


Edited by Stipe-n Cap (02/07/21 09:42 AM)

Extras: Filter Print Post Top
Offlineclockworkshroom
Stranger
 User Gallery

Registered: 02/20/20
Posts: 655
Last seen: 2 years, 9 months
Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27191319 - 02/07/21 09:54 AM (3 years, 1 month ago)

Yeah I definitely expect I am. I read Bod saying he moved from wide mouth jars to standard as less chance of contams. If someone with that much experience benefits from a small change like that or feels they need to it shows how easy it is to contam a jar when opening it.

I wipe the surface my SAB sits on with lots of iso, just a kitchen work top (no towel or anything), I wipe down the cookie rack I use with iso and also the jars, transfer plates or agar sleeve and place them on the cookie rack, same with any tools I need scalpels etc. Then I wipe the inside of the SAB with alot of iso and then place it over the rack with everything on it. Then I lysol bomb the room and leave it for a while. I wear gloves and a mask the whole time and always iso my gloves and arms if I go out the room to let the Lysol settle. I always flame the scalpel between plates.

I suspect my awkward technique and taking too long to get my agar in the jars mixed with the grain prep is what is causing my issues.


--------------------
p9hu7's clean spawn thread

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
    #27191333 - 02/07/21 09:59 AM (3 years, 1 month ago)

There's tons of info about SAB use and what not so I won't go over that here, but it could be as simple as moving too quickly and creating air currents that move contamination into your work.

Using soapy water in a spray bottle will help you trap airborne particles in your box. Make the swap, from iso in the box to soapy water and I know it will make a difference.

Extras: Filter Print Post Top
OfflineInthepit
Aum Mani Padme Hum
Male User Gallery


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 2 months, 10 days
Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
    #27191335 - 02/07/21 10:00 AM (3 years, 1 month ago)

When I made a video of working in the SAB, I learned a lot!
Peeps spotted stuff I never would have thought of.

Haha, just watched it again and it was p9 who caught me scooching down the scalpel!

Edited by Inthepit (02/07/21 10:29 AM)

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: Inthepit]
    #27191420 - 02/07/21 10:43 AM (3 years, 1 month ago)

; )

Extras: Filter Print Post Top
Offlineclockworkshroom
Stranger
 User Gallery

Registered: 02/20/20
Posts: 655
Last seen: 2 years, 9 months
Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27191517 - 02/07/21 11:32 AM (3 years, 1 month ago)

Thanks P9 I do usually spray warm water with a drop of dish soap in as well as iso before I begin, I forgot that bit. I don't think I did when I did the grains though as thought it is more likely to cause harm than good. Going to re-visit some SAB Tek threads now.


--------------------
p9hu7's clean spawn thread

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
    #27191526 - 02/07/21 11:34 AM (3 years, 1 month ago)

No need for both, soapy water in a spray bottle is plenty. Remember that you're not trying to sanitize, you're taking advantage of physics, namely gravity. It's called a "still air" box for a reason; )

I've updated the OP with some examples of clean mycelium running.

Extras: Filter Print Post Top
Offlineclockworkshroom
Stranger
 User Gallery

Registered: 02/20/20
Posts: 655
Last seen: 2 years, 9 months
Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
    #27192046 - 02/07/21 03:08 PM (3 years, 1 month ago)

Quote:

clockworkshroom said:
Ok, I'm braced and ready for the pain. First 2 photos were agar to grain and shook a couple of days ago at around 30-40%.

The other 2 rows were inoculated using a really half assed attempt at blenderless Tek with agar that was too hard and didn't break up well but may actually be healthier?

Same whole oats as the jars already shared in this thread that are bad. All prepped using bods Tek, boiled for like 40 mins and then dried for a few hours in strainers. They may be too moist.



I've bought 25kg really clean, pure, white millet and will try the no rinse Tek where you just add them and water to a jar and pc.




The taller qt jar in the last pic now has green grains and smells mouldy :smile:

I would have identified that one on my own eventually.


--------------------
p9hu7's clean spawn thread

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
    #27192051 - 02/07/21 03:10 PM (3 years, 1 month ago)

Yeah, they're all bacterial.

Edit*
Bacteria will lead to mold, now that you've seen it yourself you can make the connection between compromised jars and moldy substrates. Clean spawn is paramount.

Edited by Stipe-n Cap (02/07/21 03:18 PM)

Extras: Filter Print Post Top
Offlineclockworkshroom
Stranger
 User Gallery

Registered: 02/20/20
Posts: 655
Last seen: 2 years, 9 months
Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27192123 - 02/07/21 03:40 PM (3 years, 1 month ago)

Yes, connection made. After having no agar issues I got complacent, my next jar run is going to be planned and executed well, if I fail it will be due to a mistake that isn't sloppiness.

Do you think my plan to use millet and this Tek is a bad one?


https://www.shroomery.org/forums/showflat.php/Number/26578102/fpart/1/vc/1


--------------------
p9hu7's clean spawn thread

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
    #27192128 - 02/07/21 03:44 PM (3 years, 1 month ago)

Millet is excellent, all that's needed is to soak it for 6 - 18 hours, drain, load, pc.



No need for heat, unless that's what you like to do. I've never personally tried a tek with applied heat so I cannot comment.

Actually I'm not sure what that guy is doing in that thread, too long to read through.

Extras: Filter Print Post Top
Offlineclockworkshroom
Stranger
 User Gallery

Registered: 02/20/20
Posts: 655
Last seen: 2 years, 9 months
Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27192148 - 02/07/21 03:51 PM (3 years, 1 month ago)

That's alot of millet :smile:

There's a few very similar teks. 350ml white millet, 175ml water, pc for 90 mins, open immediately when pressure drops and bash on a tyre/shake. No rinse, no soak.

It just sounds fool proof when it comes to moisture content.

When you do yours do you literally just drain/strain? No drying?


--------------------
p9hu7's clean spawn thread

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
    #27192158 - 02/07/21 03:58 PM (3 years, 1 month ago)

I dump the grains into a brewers bag thats inside of a bucket that I drilled full of holes like a strainer. I pull the grain filled brewers bag out of the bucket and let it drain in the sink for 20 mins or so while I do some work in the lab. I load the millet while still wet to the touch.

I don't personally like the no prep approach where the water is added to the vessel and the millet hydrates in the pc. I find that it cooks together, I'm not a fan. I just soak overnight as described and it works great. Pretty much impossible to fuck up, the only way to fuck it up is if you let it sit so long that the seeds germinate.

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27192166 - 02/07/21 04:07 PM (3 years, 1 month ago)

You'll notice that some grains will looked darker than others, some will even burst, this is all fine. Things will look perfect once mixed.


Extras: Filter Print Post Top
Offlinetedoro
ToadStool Tender
Male


Registered: 02/06/15
Posts: 2,212
Last seen: 7 days, 1 hour
Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27192179 - 02/07/21 04:14 PM (3 years, 1 month ago)

I've been wondering if it might be a good idea to let my bags sit until entirely cool, to lessen the condensation after nocc'ing.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: tedoro]
    #27192184 - 02/07/21 04:18 PM (3 years, 1 month ago)

I cook during the day, say mid afternoon and then let the 75x cool overnight. Sometimes the bags are still a bit warm 24 hours later when I unload them. I just let them cool further in the flow. If you need to run multiple batches back to back you can unload and let coolnon a shelf or in a tote or something.

Definitely allow your bags to cool to room temp before inoculation, the same as you would with jars.

Extras: Filter Print Post Top
Offlineclockworkshroom
Stranger
 User Gallery

Registered: 02/20/20
Posts: 655
Last seen: 2 years, 9 months
Re: Clean spawn checklist for the new grower. [Re: tedoro]
    #27192194 - 02/07/21 04:23 PM (3 years, 1 month ago)

If I'm going to do it I'm going to do it the same way as the clean spawn warden. Bit confused though, if the bucket is full of holes how is it soaking? I assume you put it in another?


--------------------
p9hu7's clean spawn thread

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,658
Loc: Canada Flag
Trusted Cultivator
Re: Clean spawn checklist for the new grower. [Re: clockworkshroom]
    #27192201 - 02/07/21 04:28 PM (3 years, 1 month ago)

I have a bunch of normal buckets that I soak in, if I had more than a couple brewers bags I'd just put the bags in the soak buckets and yank the wet grain out.
So I just line the one with holes, dump grain x2. When those are drained I dump the drain grain back into the orange bucket, I scoop from those while I load.

If you zoom in on that pic with the buckets you'll see the blue bucket is full of holes.

Extras: Filter Print Post Top
Offlineclockworkshroom
Stranger
 User Gallery

Registered: 02/20/20
Posts: 655
Last seen: 2 years, 9 months
Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
    #27192217 - 02/07/21 04:37 PM (3 years, 1 month ago)

So do you end up draining it for over 20 mins if it sits in the holey bucket before the 20 mins in the sink. After this I'm not using any more of my credits to ask questions until my first millet run is complete and I ask for feedback :smile:


--------------------
p9hu7's clean spawn thread

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* CLEAN Spawn transfer help! Splat 1,044 2 02/26/02 03:23 AM
by Anno
* normal sour smell vs "sour rot" CrazedGoat 5,912 4 03/13/03 05:17 PM
by Apocolypse
* Sour Apple Like Smell MilhouseT1 1,413 1 03/02/02 07:26 PM
by bluhoney
* My house smells like horse shit.
( 1 2 all )
SubGen1us 3,403 20 08/31/04 02:07 AM
by NNY
* smell of straw xwave3000 590 2 03/23/04 11:13 PM
by snowspeed1
* Spawn? egolesss 2,095 18 05/30/01 03:11 PM
by BrownPastures
* Spawning Dung? Grower 853 1 06/02/01 12:11 PM
by Azure
* Blue's Poo (clean thread)
( 1 2 all )
mycofile 6,121 30 09/27/02 11:53 AM
by mycofile

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
45,441 topic views. 20 members, 191 guests and 103 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.011 seconds on 15 queries.