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Hindsight
Mad Scientist


Registered: 01/24/21
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
#27568043 - 12/03/21 06:45 PM (3 years, 1 month ago) |
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It's tough to tell from my pic if that is grain that is pressed up against the jar or if it's a contaminated spot that the mycelium refuses to touch. From the pic, it looks like the latter. Good eyes. The big thing that stands out to me from that grain is how well-defined the edges of the mycelium are around the grain.
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Smartattack
C'mon man



Registered: 12/21/18
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Loc: A thought
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Re: Clean spawn checklist for the new grower. [Re: Hindsight] 1
#27568070 - 12/03/21 07:06 PM (3 years, 1 month ago) |
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Admittedly, I very rarely get a jar with absolutely zero of those. Just the Less, the better.
-------------------- * Smarts videos
* Planet of the APES
 
I'm a fungal white supremacist.
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,259
Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: Smartattack] 1
#27568135 - 12/03/21 07:52 PM (3 years, 1 month ago) |
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Quote:
Smartattack said: I have however learned to hate seeing these.

Yeah, fuck those. At closer inspection, your Jar early has one of these.
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Hindsight
Mad Scientist


Registered: 01/24/21
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27569018 - 12/04/21 12:10 PM (3 years, 1 month ago) |
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My second tub trich'd out today. This is so bizarre. Never had an issue with trich!
Does anyone know for sure if trich can ride along on agar and on grains without being detectable, only to manifest itself in the tub?
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Smartattack
C'mon man



Registered: 12/21/18
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Re: Clean spawn checklist for the new grower. [Re: Hindsight] 1
#27569034 - 12/04/21 12:16 PM (3 years, 1 month ago) |
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Quote:
Hindsight said: My second tub trich'd out today. This is so bizarre. Never had an issue with trich!
Does anyone know for sure if trich can ride along on agar and on grains without being detectable, only to manifest itself in the tub?
For sure IMO. Seen it a thousand damn times. If that isnt how it works, then its laying in wait in the coir and doesn't kick in until spawning which is currently still readily dismissed for many reasons.
-------------------- * Smarts videos
* Planet of the APES
 
I'm a fungal white supremacist.
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
#27569107 - 12/04/21 01:06 PM (3 years, 1 month ago) |
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Thanks. I've been pasteurizing the coir using the cooler method - pour boiling water over the dry coir into a cooler, close the cooler and let sit a few hours. I think for my next boxes, I will try the same method I do for my pan subs which is a 12-hour Instapot keep-warm cycle (163F exactly).
Kind of a bummer to learn that it might not be detectable on agar or grains though.
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milkboy
Child



Registered: 05/01/21
Posts: 2,524
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
#27569109 - 12/04/21 01:07 PM (3 years, 1 month ago) |
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Could it be that the jar was just really bacterial and that invited tric when he spawned it? Ive only gotten tric once so far and it was a jar that I spawned in a faht bag because it looked bacterial as fuck and I didnt have high hopes for it to begin with.
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
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Loc: Canada
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Re: Clean spawn checklist for the new grower. [Re: milkboy] 1
#27569145 - 12/04/21 01:22 PM (3 years, 1 month ago) |
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If your jars are inoculated with trich from contaminated plates it will likely reveal itself on the grains before you're able to spawn them, so if your tub is contaminating I'd suspect bacterial spawn.
Trich in your spawn will either be from a bad plate, or a faulty filter, I'd bet on a faulty filter before worrying about a bad plate though.
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Smartattack
C'mon man



Registered: 12/21/18
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Re: Clean spawn checklist for the new grower. [Re: Stipe-n Cap]
#27569186 - 12/04/21 01:59 PM (3 years, 1 month ago) |
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Yeah bad filter is def a possibility. I'm confident in some of.my past cultures that they had ride along trich that didn't show green till after spawning. The only solution was to bin everything and start from spore.
-------------------- * Smarts videos
* Planet of the APES
 
I'm a fungal white supremacist.
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Hindsight
Mad Scientist


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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
#27569248 - 12/04/21 02:39 PM (3 years, 1 month ago) |
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Thanks guys.
Milkboy: I didn’t take a pic of the jar before putting it to bulk but I don’t think it was bacterial. IIRC it looked like the jar I posted in this thread yesterday.
P9hu7: Thanks for the tip on the filter. I have been using these dime-sized SFDs from Amazon (0.22 micron I believe) that have adhesive around the bottom edge. You stick them onto a lid over your hole and they are PCable. I’m wondering about the quality of them now, especially since some have gone through 4+ PC cycles. I just cooked 9 grain jars, half with new filter discs and half with old. I’m going to track them to see if there is a difference between contam rates for old vs new discs.
What filter discs do you guys like running? I was going with full size ones but I hated metal lids and rings so I went plastic with SHIPS and these mini filters. I will say the mini filters are much thinner than the full size SFDs I used to use.
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Smartattack
C'mon man



Registered: 12/21/18
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Re: Clean spawn checklist for the new grower. [Re: Hindsight] 1
#27569254 - 12/04/21 02:42 PM (3 years, 1 month ago) |
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The other, easier, cheaper option.
-------------------- * Smarts videos
* Planet of the APES
 
I'm a fungal white supremacist.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,234
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
#27569271 - 12/04/21 02:51 PM (3 years, 1 month ago) |
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Quote:
Smartattack said:

The other, easier, cheaper option.
question about those stuffed holes...
1) My top two jars are on their sides and I sometimes get a bit of starch in the polyfill... what then?
2) Is all polyfill the same? I just grab a pillow and steal some filling. Is that okay?
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC
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TedsDead



Registered: 01/03/17
Posts: 5,024
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Re: Clean spawn checklist for the new grower. [Re: tedoro] 1
#27569295 - 12/04/21 03:06 PM (3 years, 1 month ago) |
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I havent had any issues with that. its going to get sterilized anyways.
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros
If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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tedoro
ToadStool Tender



Registered: 02/06/15
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Re: Clean spawn checklist for the new grower. [Re: TedsDead]
#27569309 - 12/04/21 03:14 PM (3 years, 1 month ago) |
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Quote:
TedsDead said: I havent had any issues with that. its going to get sterilized anyways.
I guess my concern was after getting noc'd... that the starch could cause a pathway to contamination... something growing through the filter... maybe thats not a thing.
-------------------- --------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC
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TedsDead



Registered: 01/03/17
Posts: 5,024
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Re: Clean spawn checklist for the new grower. [Re: tedoro]
#27569341 - 12/04/21 03:36 PM (3 years, 1 month ago) |
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it could be a thing. I just havent had it happen
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros
If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Smartattack
C'mon man



Registered: 12/21/18
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Loc: A thought
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Re: Clean spawn checklist for the new grower. [Re: TedsDead]
#27569397 - 12/04/21 04:19 PM (3 years, 1 month ago) |
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It's definitely a thing. I've seen it happen where mold grows in and sporulates all over the top of the grain from inside. Pretty rare though and I don't see how it would be impossible with any other filter anyways.
-------------------- * Smarts videos
* Planet of the APES
 
I'm a fungal white supremacist.
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tedoro
ToadStool Tender



Registered: 02/06/15
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Re: Clean spawn checklist for the new grower. [Re: Smartattack]
#27569418 - 12/04/21 04:37 PM (3 years, 1 month ago) |
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and is polyfill polyfill? or are there kinds of polyfill?
-------------------- --------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC
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Enjoil



Registered: 10/29/20
Posts: 2,722
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Re: Clean spawn checklist for the new grower. [Re: tedoro] 1
#27569471 - 12/04/21 05:44 PM (3 years, 1 month ago) |
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You guys be making filters with your pillows?
A large bag is about 7 dollars isn’t it?
I saw the my-pillow crackhead try to sell pillows on tv for 89 dollars a pop last night! And he wants us to think he ain’t smoking rocks anymore. Yeah right. Sorry off topic.
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TedsDead



Registered: 01/03/17
Posts: 5,024
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Re: Clean spawn checklist for the new grower. [Re: Enjoil]
#27569508 - 12/04/21 06:26 PM (3 years, 1 month ago) |
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ya and pillows at walmart are like 2 bucks. its cheaper to buy a pillow
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros
If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Enjoil



Registered: 10/29/20
Posts: 2,722
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Re: Clean spawn checklist for the new grower. [Re: TedsDead]
#27569549 - 12/04/21 07:00 PM (3 years, 1 month ago) |
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Genius!
I had no idea they sold pillows for 2 bucks…. And I was picturing you using pillows that you already had, since dude was like why not your gunna PC that thing.ha ha
my bad I’m trippin
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