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OfflineOmnipositive
Dr. Stranger

Registered: 01/29/21
Posts: 51
Loc: MI
Last seen: 2 years, 2 months
Ultrasonic cold water extraction * 1
    #27175695 - 01/29/21 07:13 AM (3 years, 3 months ago)

I've done this twice now.

1st extracted 10g PE (failed)

2nd time extracted 1 oz fresh PE.
Got 200ml @ 26mg/ml is about a macro dose.
(I'm guessing the purity to be around 70%+ the crystals are a little brown but the crystalized well).

1 drop is 6mg microdose

260mg of crystallized psilocybin for microdoseing.

1. Grind up dry mushrooms(must use ultrasonic machine to break cells)
Or
Fresh mushrooms chopped up in cold water.

2. A drop of munaric acid

3. Optional a little alcohol since not 100% of the alkaloids are soluble in water.

4.I run my ultrasonic a couple times for 30min with the mushrooms in the jar in the
Water.

6.stir plate and magnetic bar helps increase surface area. I'd stir and put back in the ultrasonic.

4. Filter

5.repeat

6.collect all you water.

7. Evaporate in a flat bottom flask on the stove.

8. Don't evaporate all the way, it's ideal to go down to 10% by volume.

9.i evaporated 10ml in a pyrex, I got 260mg

(Next)
It seems the ideal way to extract would be cold ultrasonic extraction of dried mushrooms and then evaporate by pulling vacuum. If you can keep it under room temp you can keep the psilocybin from going through the cascading 💙 reaction. This will surely yealid a more potent product.


Edited by Omnipositive (01/29/21 07:39 AM)

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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Ultrasonic cold water extraction [Re: Omnipositive]
    #27175736 - 01/29/21 07:50 AM (3 years, 3 months ago)

Why did your first extract fail? How much are those ultrasonic things? And is it really necessary is people are getting full effects from regular tea...?



Did you use a vacuume for this? Have you tried the product? Yields seem about right, around 1%>.. what is the cascading reaction?

I've been trying to find the best extraction myself, so I can put the concentrate into honey and preserve it, I think this could be a thing

I don't really need a flat bottom flask for this?

I saw this on the internet somewhere already, actually just within the last couple days .

I'm intrigued.


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OfflineOmnipositive
Dr. Stranger

Registered: 01/29/21
Posts: 51
Loc: MI
Last seen: 2 years, 2 months
Re: Ultrasonic cold water extraction [Re: QM33]
    #27175752 - 01/29/21 08:07 AM (3 years, 3 months ago)

It failed because I went to remove the waxes with naptha and used toulne by mistake.(I don't think further purification is necessary especially with harsh solvents.)

There about $80-$200

I dunno, I just did it because I had some damaged fruits.

I've micro dosed it a few times. I haven't been in the right state of mind or had the time to macro dose.

No I didn't pull vaccum on this, and there is a possibility I lost some alkaloids while heating since now that I think about it, psilocybin has a high  boiling point but I'm not sure of the indego related molecules. I could have possibly cooked everything out.

So I would re-affirm the best way would be a 100% cold water/alcohol mix with munaric acid to a ph of 3. Break the cells with ultrasonic, on a stir plate.filter or vaccum filter, evaporate under vaccum under room temp.(the first extract went from brown water at 10degree to blue at 75degrees than when I crashed the crystals out, it went brown again. That is the cascading 💙 reaction. Psilocybin turns into a molecule close to the molecule indigo.


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OfflineOmnipositive
Dr. Stranger

Registered: 01/29/21
Posts: 51
Loc: MI
Last seen: 2 years, 2 months
Re: Ultrasonic cold water extraction [Re: QM33]
    #27175753 - 01/29/21 08:08 AM (3 years, 3 months ago)

You could then cover the crystals in honey to protect them from oxidizeing.


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OfflineOmnipositive
Dr. Stranger

Registered: 01/29/21
Posts: 51
Loc: MI
Last seen: 2 years, 2 months
Re: Ultrasonic cold water extraction [Re: Omnipositive]
    #27175988 - 01/29/21 10:33 AM (3 years, 3 months ago)

The more I think about this, it's probably a fail. I just extracted at 40-50 degrees everything oxidized.

This extraction has to be done really cold.


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InvisibleQM33
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Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Ultrasonic cold water extraction [Re: Omnipositive]
    #27176107 - 01/29/21 11:42 AM (3 years, 3 months ago)

Hmmmmmm....... I've used dry ice to super chill everclear for my bho. I'll blast directly into the super chilled mixture. I've made has extracts like this also.......
Can this be utilized


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Ultrasonic cold water extraction [Re: QM33]
    #27176111 - 01/29/21 11:44 AM (3 years, 3 months ago)

Celsius?


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
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OfflineOmnipositive
Dr. Stranger

Registered: 01/29/21
Posts: 51
Loc: MI
Last seen: 2 years, 2 months
Re: Ultrasonic cold water extraction [Re: QM33]
    #27176331 - 01/29/21 02:21 PM (3 years, 3 months ago)

Here's the reaction that happens to psilocybin when it oxidizes. We just need to keep the temperature cold. I did my first one right, I extracted at about 10defrees outside. The psilocybin extracted into the water without turning blue. You have to pull vaccum on the water while it's cold to keep it from oxidizeing. Dry ice can be used, but it's winter here, I'm just going to do mine outside or in a mini freezer I've got.

https://images.app.goo.gl/7GXyBDE9wTEoGn7D6


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Edited by Omnipositive (01/29/21 02:28 PM)

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Offlinefushoomushoo
Stranger
Registered: 03/07/12
Posts: 33
Last seen: 1 year, 10 months
Re: Ultrasonic cold water extraction [Re: Omnipositive]
    #27221052 - 02/22/21 08:26 PM (3 years, 2 months ago)

Did you defat in your last attempt?

would re-dissolving in 100% alcohol and storing in an amber bottle help to prevent oxidization?

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Offlinefushoomushoo
Stranger
Registered: 03/07/12
Posts: 33
Last seen: 1 year, 10 months
Re: Ultrasonic cold water extraction [Re: fushoomushoo]
    #27221080 - 02/22/21 08:51 PM (3 years, 2 months ago)

Let me know what you think of this as a procedure:

Quote:

1(A). Grind up 10g dry mushrooms. and add to 180ml of cold water.

Use ultrasonic cleaner to break cells.

If you don't have access to an ultrasonic cleaner, hydrate dried mushroom powder in room temperature water and leave until fully hydrated, freeze slurry, thaw. Repeat freeze & thaw several times.

1(B). Add 100g fresh mushrooms finely chopped to 110ml of cold water.



2. Add munaric acid to lower PH to 3.

3. Add 20ml ethanol since 100% of the alkaloids are not soluble in water.

4. Place vessel on stir plate and mix liquid for 30 minutes to help alkaloids migrate to the water/ethanol.

5. Filter under vacuum, store water/ethanol.

 

6. Repeat steps 1 to 4 with remaining mushroom powder from filter, the more times you complete this process, the more you will get out of your starting product (with diminishing returns).

7. Combine all water/ethanol from extractions and mix with 100ml of non-polar solvent (shellite). Allow fluid to mix on stir plate for 30 minutes.

8. Separate water/ethanol from solvent (separatory funnel or chemical-resistant pipette), discard solvent.

9. Boil away water/ethanol at room temperature by applying vacuum.





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OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,280
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Re: Ultrasonic cold water extraction [Re: fushoomushoo]
    #27230233 - 02/28/21 07:46 AM (3 years, 2 months ago)

I recall reading from a scholarly article somewhere that light is the biggest degrader of aqueous psilocybin solutions. Maybe thats whats ruining your attempts.

I'm no chemist but I saw some people discussing another psil extraction idea saying that acid will convert the psilocybin to psilocin which they claimed to be less stable than psilocybin.

Another thing that might work is alkaline water, kagan water. Never saw it for myself but my friends claimed you could brew a cup of cold tea with alkaline water and it acts as if it were hot water. that and vacuum  evaporation, all in a perfectly dark environment may work. Or perhaps there's something you could add to solution to somehow stabilize the goodies

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InvisibleSpaceCube
π

Registered: 06/02/21
Posts: 153
Re: Ultrasonic cold water extraction [Re: Psilosopherr]
    #27348431 - 06/14/21 08:33 PM (2 years, 10 months ago)

Bump, this is the type of post that needs enhanced research.

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Offliner35l
innoculatory engineer

Registered: 07/18/16
Posts: 34
Last seen: 1 year, 5 months
Re: Ultrasonic cold water extraction [Re: Omnipositive]
    #27398489 - 07/22/21 08:42 PM (2 years, 9 months ago)

Quote:

Omnipositive said:
.

So I would re-affirm the best way would be a 100% cold water/alcohol mix with munaric acid to a ph of 3. Break the cells with ultrasonic, on a stir plate.filter or vaccum filter, evaporate under vaccum under room temp.(the first extract went from brown water at 10degree to blue at 75degrees than when I crashed the crystals out, it went brown again. That is the cascading 💙 reaction. Psilocybin turns into a molecule close to the molecule indigo.






Are you meaning “fumaric acid” or “Muriatic acid” …I’ve never heard of munaric acid

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OfflineBobbit
Even Stranger


Registered: 05/30/18
Posts: 1,251
Loc: The outsideround
Last seen: 11 days, 2 minutes
Re: Ultrasonic cold water extraction [Re: Psilosopherr]
    #27713631 - 03/30/22 03:45 AM (2 years, 1 month ago)

Quote:

Psilosopherr said:
I recall reading from a scholarly article somewhere that light is the biggest degrader of aqueous psilocybin solutions. Maybe thats whats ruining your attempts.

I'm no chemist but I saw some people discussing another psil extraction idea saying that acid will convert the psilocybin to psilocin which they claimed to be less stable than psilocybin.

Another thing that might work is alkaline water, kagan water. Never saw it for myself but my friends claimed you could brew a cup of cold tea with alkaline water and it acts as if it were hot water. that and vacuum  evaporation, all in a perfectly dark environment may work. Or perhaps there's something you could add to solution to somehow stabilize the goodies




So. . .
I’ve ready a bunch of scholarly articles that suggest methanol as a solvent for psilocybin. Room temp for 12hr or 45°C for 1hr. Once dried, the resulting honey goo WILL NOT DISSOLVE in cold 93% ethanol (or cold dry acetone, or cold isopropanol), and requires water. I use 40% ethanol and it works well. Coffee filter out the wax once resolved.

The challenge with aqueous extractions seems to be the phosphatase enzymes present in all psilocybin containing mushrooms. So Ethanol, water or acidic extracts all pull the phosphatases, acids also dephosphorylate themselves. Psilocin is particularly susceptible to oxydation. The blueing you see in fresh mushrooms, is a byproduct of psilocin oxidation.

If you are a biochemist and can turn psilocin into a stable salt, acid extraction is your pathway, though you need to know what you are doing.

UV certainly has an impact on methanol extraction resolved in 40% Ethanol, which turns from urine coloured to dark brown with exposure to sunlight.

You can resolve the extract in methanol at 60°C or Ethanol  at 80°C. These temperatures fail to resolve in acetone or Isopropanol. And nothing freeze precipitates out?

Ongoing experiments will hopefully yield more data.


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OfflineRotnpins
🤮 Rotten-Pins 🍄
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Registered: 01/11/22
Posts: 4,738
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Re: Ultrasonic cold water extraction [Re: Bobbit]
    #27715408 - 03/31/22 01:04 PM (2 years, 1 month ago)

:takingnotes:

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OfflineIsztwan
Stranger
Registered: 03/22/20
Posts: 21
Last seen: 11 months, 22 days
Re: Ultrasonic cold water extraction [Re: Rotnpins]
    #27721904 - 04/05/22 09:24 AM (2 years, 1 month ago)

Sorry to say this but most of ultrasonic cleaners use 40kHz frequency.

Prolonged time of extraction using that freq cause many organic substances to disbond ( aka RIP ).

With customer grade Ultrasonics you need to find one that have dual freq. aka 28kHz and 40kHz and minimum 240W US power.

For extraction you want to use 28kHz.

And for f sake don't use here HCl to lower pH, USE Phosphoric acid instead.
(HCl will F phosphate groups that stabilizes psylocybin!)

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