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OfflineD_T.eonanacatl
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Re: Roars APE grow along/comparative 2021! [Re: Josex]
    #27167507 - 01/24/21 06:06 PM (1 month, 9 days ago)

I missed out on LAGM. This could be fun too.

Jan 24: Agar plates swabbed, last one cut small strand into.

Feb 2: First signs of germ on strands
Feb 4: Growth spreading!


Feb 5: First Transfer
Feb 13: T1 growing uneven

Uneven growth made for stalled out T2, time to backtrack to germ plate

Feb 17: another T1 plate made
Feb 22: looking better. Second Transfers cut.



Edited by D_T.eonanacatl (02/23/21 11:38 AM)


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OfflineDoctor MarioS
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Re: Roars APE grow along/comparative 2021! [Re: D_T.eonanacatl]
    #27167596 - 01/24/21 06:56 PM (1 month, 8 days ago)

No you didn't. LAGM is open all year. Just tell Gypsy what varieties you want to enter with.


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OfflineroarkellS
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Re: Roars APE grow along/comparative 2021! [Re: Doctor Mario]
    #27167635 - 01/24/21 07:14 PM (1 month, 8 days ago)

Why not do both? Lol


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OfflineInthepit
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Re: Roars APE grow along/comparative 2021! [Re: roarkell]
    #27167707 - 01/24/21 07:55 PM (1 month, 8 days ago)

Two plates for the APE swab: one contam and one a light circle of growth??
Next time I use a swab going for more plates for that swab.

14 January, 2021:
Well...
       

Progress is progress, no matter how small...  :lol:

20 January, 2021:
From five plates with four wedges ea. Transferred two plates, 4 wedges ea. of SW APE for T2.

Well I think I'm learning. I'm realizing it's a numbers game.
I've started putting four wedges per plate. So last one was 20 wedges,
so it's like 20 plates! But only five.
And out of that I managed to go T2, two plates for 8 chances at a winner!

Also I've started looking at the growth with a strong magnifying glass from
the back as the growth shows better. Learning to look for straighter, better organized.


21 January, 2021:
Ok, swabbed four plates, including the swab tip last one.
So, second try. Nothing to see yet...

28 January, 2021:
So has anyone else looked at their swab doing nothing in the plate and
ripped it out and shredded it to three other plates?  :lol:

                          7 days old
And just look at the contam coming from behind where I held the swab.

Then I had a second thought that I should have put a drop of water on each.
Anyway just a noob larnin 'bout swabbin!

(ya really lumpy green agar, more larnin!  )
10g 2% Agar, 7.5g 1.5% LME,  500ml 12/30 pretty hard
Split 2 jars grn & blu/red πŸ’ 325ml


28 January, 2021:


Edited by Inthepit (01/28/21 07:50 AM)


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OfflineDoctor MarioS
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Re: Roars APE grow along/comparative 2021! [Re: Inthepit]
    #27167803 - 01/24/21 08:41 PM (1 month, 8 days ago)

Still waiting for mine to germ 😬


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OfflineroarkellS
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Re: Roars APE grow along/comparative 2021! [Re: Doctor Mario]
    #27167892 - 01/24/21 09:41 PM (1 month, 8 days ago)

Being stubborn, eh? I haven't tested any of the most recent batch on agar yet... I should run a control plate or 2...


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OfflineDoctor MarioS
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Re: Roars APE grow along/comparative 2021! [Re: roarkell]
    #27167923 - 01/24/21 10:00 PM (1 month, 8 days ago)

Eh, it's only been 4 days and burying swabs seems to always be 3-5 days slower than loop streaks. I'll give it another 5 days and if I don't see anything, I'll put a drop of sterilized water over each part of the buried swab and see if that makes em pop.


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InvisibleScrimshaw
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Re: Roars APE grow along/comparative 2021! [Re: Doctor Mario]
    #27168934 - 01/25/21 01:36 PM (1 month, 8 days ago)

1/25/2021

Rolled one swab to two PastyPlates. Temps will be in the mid to upper 60s for germination. I will keep this thread updated once I have growth.

1/29/2021

I took Roar’s advice and tossed a space heater in my germination room to get my temps up. I noticed a tiny white spot on one of my plates yesterday which if it’s not a contam, would be 3 days to germinate. It’s slightly off center of where I swabbed.

Now that some of the condensation has cleared up in my plates, the exact center of the plate where I first made contact with the swab is starting to look like germination may be starting there. I will know for sure by the end of the weekend.

2/1/2021

The small dot on one of my plates hasn’t changed at all since my last post. No growth, no color change, no nothing. Still no idea if it’s a contam or spore germination. I will monitor. Nothing on the other plate.

In the meantime, I took the remaining swab and streaked a few more plates and buried the swab in one of them.

Hoping for some success on this round.

2/7/2021

We have liftoff! The only growth I see is in the plate where I buried the swab.



--------------------


Edited by Scrimshaw (02/07/21 11:58 AM)


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OfflineroarkellS
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Re: Roars APE grow along/comparative 2021! [Re: Scrimshaw]
    #27168951 - 01/25/21 01:42 PM (1 month, 8 days ago)

Quote:

Scrimshaw said:
1/25/2021

Rolled one swab to two PastyPlates. Temps will be in the mid to upper 60s for germination. I will keep this thread updated once I have growth.




You might wanna find a warmer spot to set them until you get some germination... APE  are notoriously slow and a lot of people have some difficulty getting germination from swabs in general... just my 2 cents tho... hopefully you get something to pop...


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Invisiblecapntrips7
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Re: Roars APE grow along/comparative 2021! [Re: roarkell]
    #27172049 - 01/27/21 02:08 AM (1 month, 6 days ago)

Thanks for the swabs, roar!

I put one of the swab tips in the bottom of measuring cup and repeatedly sprayed it with 30ml of distilled water from a syringe, an extreme bastardization of faht's tek. After a few cycles, I put a drop in an inoculation loop and streaked a pair of plates. Then I cut the tip in half and streaked another pair of plates. All 4 plates were 1% agar, 1.5% LME. I'll hold onto the other swab, in case this doesn't work out.

I'm also tracking this grow in LAGM2021.


--------------------
LAGM2021


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OfflineTankie_J
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Re: Roars APE grow along/comparative 2021! [Re: capntrips7]
    #27172072 - 01/27/21 02:37 AM (1 month, 6 days ago)

Just saw this pop up. I’m jumping in on this, my swabs are from Roar through a trade. Took some effort to germ but I have 8 colonizing grain jars now. All have been shook and should be ready for spawn in the next couple weeks depending.


Pre Shake (20JAN)


Recovery on 23JAN

I shook again yesterday to speed things up a bit. Recovery looks good, no pic yet.

All jars were noc’d with small agar wedges and took forever to colonize, I would recommend using larger or more pieces for faster colonization.


--------------------
LAGM2021
List of teks I use


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OfflineInthepit
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Re: Roars APE grow along/comparative 2021! [Re: roarkell]
    #27174051 - 01/28/21 07:45 AM (1 month, 5 days ago)

So has anyone else looked at their swab doing nothing in the plate and
ripped it out and shredded it to three other plates?  :lol:

                          7 days old
And just look at the contam coming from behind where I held the swab.

Then I had a second thought that I should have put a drop of water on each.
Anyway just a noob larnin 'bout swabbin!

(ya really lumpy green agar, more larnin!  )
10g 2% Agar, 7.5g 1.5% LME,  500ml 12/30 pretty hard
Split 2 jars grn & blu/red πŸ’ 325ml


--------------------
GLOSSARY        Getting Started      :chugbeer:        My LAGM21    Do you exist?


Edited by Inthepit (01/28/21 07:47 AM)


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Offlineanatomality
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Re: Roars APE grow along/comparative 2021! [Re: Inthepit]
    #27174064 - 01/28/21 08:06 AM (1 month, 5 days ago)

I'll hop in when I get my Roarin' swab.
I've been having troubles with germinating swabs, poured from softer agar yesterday... Will report back!


--------------------
Check out my LAGM2021 log here.
β€œThe strength of a person's spirit would then be measured by how much 'truth' he could tolerate, or more precisely, to what extent he needs to have it diluted, disguised, sweetened, muted, falsified.”


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Offlinestarbones
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Re: Roars APE grow along/comparative 2021! [Re: Inthepit] * 1
    #27174068 - 01/28/21 08:08 AM (1 month, 5 days ago)

Was frustrated at germing plates with swabs over the past couple months and got tired of just burying the whole swab in the plate so I started to use a fresh sterile 28g needle to rip a tuft of spore laden cotton off the swab which I then drag down into the middle of a thickly poured plate so it's sammiched around with agar like the tip of a buried swab would get.

Works like a hot damn.


--------------------
Calm as cookies and cream.
Unbottles and micro-mini-mono tubs all day everyday.



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OfflineDoctor MarioS
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Re: Roars APE grow along/comparative 2021! [Re: starbones]
    #27175231 - 01/28/21 10:30 PM (1 month, 4 days ago)

About how long until you guys are seeing germination? Mine still aren't growing anything except for a really small spot blue bacteria.


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OfflineTankie_J
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Re: Roars APE grow along/comparative 2021! [Re: Doctor Mario]
    #27175281 - 01/28/21 11:13 PM (1 month, 4 days ago)

I didn’t document a timeline on germination :facepalm:

I am having good germ luck with an 8g lme 8g agar to 400ml distilled water recipe in combination with dipping the swab in sterile water first.
Another good tek with swabs has been using this brf puck recipe and taking a few strands off the swab and shoving it in the puck.

Best of luck to you guys!


--------------------
LAGM2021
List of teks I use


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OfflineroarkellS
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Re: Roars APE grow along/comparative 2021! [Re: Tankie_J]
    #27175533 - 01/29/21 05:50 AM (1 month, 4 days ago)

I've been swimming in work the past few weeks... if I get some free time tonight, I'll swab another round of these just so you guys can have a look at how I do shit... hopefully that'll help!


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OfflineInthepit
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Re: Roars APE grow along/comparative 2021! [Re: roarkell]
    #27175551 - 01/29/21 06:14 AM (1 month, 4 days ago)

I haven't been getting anything, but I'm so noob.

Container label Date Comments
2 plates  swab 1 1/1 one plate brown glossy bacterial at swab  10g 2% Agar, 7.5g 1.5% LME, 500ml
4 plates swab 2 1/21 4 plates plus the tip, Roar
3 plates swab 2 1/28 Shredded swab2 to 3 plates

I'm tempted to add a drop of water to the swab tip shreds,
to give the spores a drink!


--------------------
GLOSSARY        Getting Started      :chugbeer:        My LAGM21    Do you exist?


Edited by Inthepit (01/29/21 06:17 AM)


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OfflineroarkellS
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Re: Roars APE grow along/comparative 2021! [Re: Inthepit]
    #27175639 - 01/29/21 08:26 AM (1 month, 4 days ago)

I dont usually have issues with spore hydration... I don't even alter my agar recipe for germ plates...


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Offlineanatomality
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Re: Roars APE grow along/comparative 2021! [Re: roarkell]
    #27176031 - 01/29/21 12:54 PM (1 month, 4 days ago)

Got my swabs! Going to pop them on agar next time I set up the SAB.
Stoked!


--------------------
Check out my LAGM2021 log here.
β€œThe strength of a person's spirit would then be measured by how much 'truth' he could tolerate, or more precisely, to what extent he needs to have it diluted, disguised, sweetened, muted, falsified.”


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