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nalakava
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Registered: 11/18/20
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Help needed: Cloning to agar to grain
#27159669 - 01/20/21 02:08 PM (3 years, 2 months ago) |
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So I cloned some shrooms I grew a few weeks back following Frank's Proper Cloning Tek I had to leave the plate growing for two weeks while I went on a ski trip. When I came back, I transferred 8 sectors into new blank plates using D3's Perfect Transfers Tek This is the plate after I took out the circles:
Now it's been three days since I did that first transfer and this is how all plates look like:
Here's closer pics of each plate:
What are my next steps? Should I pick my best plate and work from there?
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mushhead
Potato Devourer
Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
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Re: Help needed: Cloning to agar to grain [Re: nalakava]
#27159675 - 01/20/21 02:12 PM (3 years, 2 months ago) |
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I would allow them a few days growth, discern by visual cues the "nice" looking mycelium. I say "nice" because its all subjective to the genetics you're working with. after you've selected which plates you want to xfer from I'd take the xfer but then also use the same plate for a jar. this way I have the transfer going on agar and also a jar in the colonization stage. The jar gets used in a shoebox where I then chose another clone from a cluster. rinse and repeat.
Quote:
nalakava said:
4 O'clock position on the growth within this plate looks great to transfer with.
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Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
Edited by mushhead (01/20/21 02:24 PM)
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tiptrippy
The Mechanic
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Re: Help needed: Cloning to agar to grain [Re: nalakava]
#27159678 - 01/20/21 02:13 PM (3 years, 2 months ago) |
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Let them grow out a bit then drop like 4 wedges in each quart size grain jar since you have so many plates. Use each jar as a master jar and g2g them to 10 other jars each. That will give you 50 quarts of spawn easily or you can take 10 of them and use them as masters to make another 100 quarts of spawn. All with the same genetics as that clone.
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mushhead
Potato Devourer
Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
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Re: Help needed: Cloning to agar to grain [Re: tiptrippy]
#27159687 - 01/20/21 02:18 PM (3 years, 2 months ago) |
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Quote:
tiptrippy said: Let them grow out a bit then drop like 4 wedges in each quart size grain jar since you have so many plates. Use each jar as a master jar and g2g them to 10 other jars each. That will give you 50 quarts of spawn easily or you can take 10 of them and use them as masters to make another 100 quarts of spawn. All with the same genetics as that clone.
before running through this process it would be advisable to narrow down a little. The clone obviously has over thousands of different genetics within it. removing a few of them by transfer and shoebox fruiting greatly increases chances of high yield and at least for me in many cases high potency.
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tiptrippy
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Posts: 1,131
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Last seen: 1 year, 8 months
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Re: Help needed: Cloning to agar to grain [Re: mushhead] 1
#27159692 - 01/20/21 02:20 PM (3 years, 2 months ago) |
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Quote:
mushhead said:
Quote:
tiptrippy said: Let them grow out a bit then drop like 4 wedges in each quart size grain jar since you have so many plates. Use each jar as a master jar and g2g them to 10 other jars each. That will give you 50 quarts of spawn easily or you can take 10 of them and use them as masters to make another 100 quarts of spawn. All with the same genetics as that clone.
before running through this process it would be advisable to narrow down a little. The clone obviously has over thousands of different genetics within it. removing a few of them by transfer and shoebox fruiting greatly increases chances of high yield and at least for me in many cases high potency.
Yeah this is probably a better idea haha
I get so excited when I see a bunch of clean myc on agar I just wanna go straight to grain
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nalakava
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Registered: 11/18/20
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Re: Help needed: Cloning to agar to grain [Re: tiptrippy] 1
#27159700 - 01/20/21 02:25 PM (3 years, 2 months ago) |
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Thank you all so much for the help. So I give plate 2 a few days. After I transfer from it, what would you do with the other plates?
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mushhead
Potato Devourer
Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
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Re: Help needed: Cloning to agar to grain [Re: tiptrippy]
#27159701 - 01/20/21 02:25 PM (3 years, 2 months ago) |
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Quote:
tiptrippy said:
Quote:
mushhead said:
Quote:
tiptrippy said: Let them grow out a bit then drop like 4 wedges in each quart size grain jar since you have so many plates. Use each jar as a master jar and g2g them to 10 other jars each. That will give you 50 quarts of spawn easily or you can take 10 of them and use them as masters to make another 100 quarts of spawn. All with the same genetics as that clone.
before running through this process it would be advisable to narrow down a little. The clone obviously has over thousands of different genetics within it. removing a few of them by transfer and shoebox fruiting greatly increases chances of high yield and at least for me in many cases high potency.
Yeah this is probably a better idea haha
I get so excited when I see a bunch of clean myc on agar I just wanna go straight to grain
me too and so do many others, no problem with it either, I just like to get uniform canopies.
Quote:
nalakava said: Thank you all so much for the help. So I give plate 2 a few days. After I transfer from it, what would you do with the other plates?
Give them the same chance as you would give plate 2. with plates I don't xfer from and they win out on the plate, meaning they've finished colonizing the entire plate, I usually send it to grain no matter how it looks as long as its clean mushroom mycelia. If they win the plate, they get to fruit.
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nalakava
Stranger
Registered: 11/18/20
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Last seen: 2 years, 14 days
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Re: Help needed: Cloning to agar to grain - Picture Updates [Re: mushhead]
#27167275 - 01/24/21 01:26 PM (3 years, 2 months ago) |
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It's been 4 days since the first post. I'm gonna do my transfers today, but I wanted to show yall how it's looking:
This is plate 1:
This is plate 2:
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tiptrippy
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Registered: 09/09/20
Posts: 1,131
Loc: United States
Last seen: 1 year, 8 months
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Re: Help needed: Cloning to agar to grain - Picture Updates [Re: nalakava]
#27167719 - 01/24/21 06:00 PM (3 years, 2 months ago) |
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Both of those look great to me.
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