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Crackatoa
Stranger in a strange land
Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house
Last seen: 24 days, 1 hour
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Gypsum hasn't shown to be beneficial. Unless you throw some in a grain for extra moisture control and nobody hardly does that.
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verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 101
Last seen: 3 years, 1 month
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Quote:
Crackatoa said: Gypsum hasn't shown to be beneficial. Unless you throw some in a grain for extra moisture control and nobody hardly does that.
Wow I swear it was considered essential like 5 years ago haha. Okay I'll knock that off until further notice!
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openmyc
Primordium
Registered: 10/26/20
Posts: 18
Last seen: 3 years, 1 month
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Quote:
verytastycheese said: A wild contam appears?]
I’m new to this; are you sure that’s contam and not bruising?
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Justweed
Bobby's short shorts
Registered: 04/01/20
Posts: 555
Last seen: 29 days, 48 minutes
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Quote:
Professor X said: If it's coir verm you can use cold tap water to prep. Yes the mold is in your jars, could be in your culture.
Not 100%. I get verm from a grow store and I've had it carry contams. I've also had gypsum lead to contaminants from that store (added the gypsum after the boiling water in the substrate mix had cooled, 8 tubs down the drain). Some vendors just have contaminated areas. I also once got a pack of stick on lights that I decided to try putting inside my tubs, each one tub that got a light got trich. I didn't think I'd need to wipe down a plastic surface straight out of the packaging but I should have! Trich can hide everywhere. If there's a chunk on the outside of the coir or verm packaging, and you handle that before putting water with it, you're likely to get trich.
-------------------- Keep 'em high and tight guys....
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openmyc
Primordium
Registered: 10/26/20
Posts: 18
Last seen: 3 years, 1 month
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Re: What happened? [Re: Justweed]
#27163229 - 01/22/21 10:24 AM (3 years, 2 months ago) |
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Damn. Sorry to hear about your vendor trich-Trojan horse. I’ve been anal about sanitizing everything that might even come near a clean environment, I guess that’s a carryover from homebrewing. I’ve seen so many people around here toss weeks or months of progress at the first sight of blue/green, at others’ recommendation—and then another will chime in saying it was just bruising or penicillium, and that all would have been fine.
I understand erring on the side of caution with a contam that has the potential to sporulate and quickly ruin an entire room or lab. I guess I need to invest in a microscope next.
Keep up the good work.
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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Re: What happened? [Re: Justweed]
#27163266 - 01/22/21 10:41 AM (3 years, 2 months ago) |
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Quote:
verytastycheese said: Still inconsequential, at least I know the genetics will pin. But back to the theory that if you get contams before first flush it must be in your spawn? No chance it was introduced by an imperfect spawning or pasteurization of substrate? What I'm learning here is I probably need to shake my jars more to potentially reveal contams?
Moreover, I should move to liquid. I'll be reading up on your tek Josex.
Here's the jars 3 days after I shook them. At what point would you call them fully colonized and ready to spawn?
Most likely it was your spawn, yes. The good thing about knowing that it's always likely your spawn is that it narrows down the number of plausible vectors so you don't waste time looking into far more unlikely causes (like the coir or "the air", for instance), although it's never smart to rule out anything completely however unlikely.
Coir doesn't need pasteurization at all. Just put it in a bucket with the right amount of boiling water and you're set, Some people use cold tap water too with success but I prefer to boil it first. Also, gypsum is not necessary at all.
I don't like how these jars recovered from the shake, not saying they're totally fucked but they look a bit bacterial to me. The curious thing about shaking at 100% (or nearly) is that it tells you the whole story, warts and all. Maybe the jar was looking good before a shake but it doesn't look so good after it. Bacterial spawn can weaken the colony and this in turn can give mold a chance to get a foothold when spawned, so this is something to consider, although I'm not saying those jars look so bad that they'll likely give ya mold nor am I saying bacterial spawn was the cause of your mold problems.
By all means shake the jars more often. You had some mold in your jars before that looked as if it was introduced via technique. Mold in your jars can easily go unnoticed sometimes but a shake can reveal it.
The things that can get your spawn looking bacterial are a bad prep (too wet or too dry) or an insufficient cycle, your inoculant and your technique. Your inoculant (agar) looks good to me. Introducing bacteria from technique in your jars is not very likely IMO but it can happen. How's your prep?
I wouldn't think of using LC until you fix your issues first.
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openmyc
Primordium
Registered: 10/26/20
Posts: 18
Last seen: 3 years, 1 month
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Re: What happened? [Re: Josex]
#27163301 - 01/22/21 10:54 AM (3 years, 2 months ago) |
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Quote:
Josex said:
I don't like how these jars recovered from the shake, not saying they're totally fucked but they look a bit bacterial to me.
Not OP, but curious because my own jars looked similar: can you help me understand what looks bacterial about these? Is it the amount of rhizomorphic/tomentose growth or something more subtle?
Thanks for your help.
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verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 101
Last seen: 3 years, 1 month
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Re: What happened? [Re: openmyc]
#27165970 - 01/23/21 06:32 PM (3 years, 2 months ago) |
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Quote:
openmyc said:
Quote:
Josex said:
I don't like how these jars recovered from the shake, not saying they're totally fucked but they look a bit bacterial to me.
Not OP, but curious because my own jars looked similar: can you help me understand what looks bacterial about these? Is it the amount of rhizomorphic/tomentose growth or something more subtle?
Thanks for your help.
I'm also curious if you could be more specific. Those jars looked the best I've ever seen personally, but I may not have ever seen the holy grail.
Here's a pic from today
Quote:
openmyc said:
Quote:
verytastycheese said: A wild contam appears?]
I’m new to this; are you sure that’s contam and not bruising?
Nope, it turned to classic trich shortly after. Still popping a few pins slowly, quarantined elsewhere, but dead.
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openmyc
Primordium
Registered: 10/26/20
Posts: 18
Last seen: 3 years, 1 month
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Sorry to hear it, verytasty. Thanks for updating and helping me learn.
For what it’s worth that jar looks happy and healthy to me. You should be a proud spawn parent. 👶
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verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 101
Last seen: 3 years, 1 month
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Re: What happened? [Re: openmyc]
#27166020 - 01/23/21 07:05 PM (3 years, 2 months ago) |
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Quote:
openmyc said: Sorry to hear it, verytasty. Thanks for updating and helping me learn.
For what it’s worth that jar looks happy and healthy to me. You should be a proud spawn parent. 👶
I still think it is. I'm hoping it was just the lighting I took the previous picture in, it was a different color temp and might have thrown him off or something. But it still has me head scratching.
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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Re: What happened? [Re: openmyc]
#27166029 - 01/23/21 07:11 PM (3 years, 2 months ago) |
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I may be a bit too picky. One thing is for sure, perfect spawn is the hardest thing to achieve in this hobby. I'm not sayin your jars look bad, I actually think they're spawn-able, it's just that I think they look a bit bacterial but not the end of the world.
Myc looks a bit stressed and I don't say that for those rhizos that is shooting out (this can be considered an indication of bacterial spawn in some cases) because your cultures have been throwing out some crazy rhizos on agar too so I don't know what to think about it tbh. They're covering the grains too lightly to my liking to the point they almost look uncolonized, that's the part I like less about those jars. Growth is also patchy and not uniform.
Edited by Josex (01/23/21 07:34 PM)
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